(Circular) (Six-base) MAPSORT of: pFN21AB8942.seq Check: 9183 from: 1 to: 5608 pFN21AB8942 5608 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2422 2695 2786 2422 Size: 273 91 5244 Fragments arranged by size: 5244 273 91 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3418 278 Size: 53 83 186 936 1882 2468 Fragments arranged by size: 2468 1882 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2951 1937 Size: 1014 4594 Acc65I G'GTAC_C Cuts at: 2469 2929 2469 Size: 460 5148 AclI AA'CG_TT Cuts at: 1563 3735 4108 1563 Size: 2172 373 3063 Fragments arranged by size: 3063 2172 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3671 5055 5323 1632 Size: 2039 1384 268 1917 Fragments arranged by size: 2039 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5411 AflIII A'CryG_T Cuts at: 1203 4507 5484 1203 Size: 3304 977 1327 Fragments arranged by size: 3304 1327 977 AgeI A'CCGG_T Cuts at: 4439 4439 Size: 5608 AhdI GACnn_n'nnGTC Cuts at: 4337 4337 Size: 5608 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5608 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5608 AlwNI CAG_nnn'CTG Cuts at: 2134 2437 2904 3351 4923 2134 Size: 303 467 447 1572 2819 Fragments arranged by size: 2819 1572 467 447 303 ApaI G_GGCC'C Cuts at: 2672 2679 2672 Size: 7 5601 ApaLI G'TGCA_C Cuts at: 3665 4821 3665 Size: 1156 4452 ApoI r'AATT_y Cuts at: 1493 2917 3143 3295 5404 1493 Size: 1424 226 152 2109 1697 Fragments arranged by size: 2109 1697 1424 226 152 AscI GG'CGCG_CC Cuts at: 2790 2790 Size: 5608 AseI AT'TA_AT Cuts at: 160 1032 4162 160 Size: 872 3130 1606 Fragments arranged by size: 3130 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5608 AvaI C'yCGr_G Cuts at: 1870 1943 2483 2672 2797 2933 1870 Size: 73 540 189 125 136 4545 Fragments arranged by size: 4545 540 189 136 125 73 BamHI G'GATC_C Cuts at: 2938 3305 2938 Size: 367 5241 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2323 2469 2774 2929 4384 Size: 325 217 627 536 146 305 155 1455 Cuts at: 4384 618 Size: 1842 Fragments arranged by size: 1842 1455 627 536 325 305 217 155 146 BanII G_rGCy'C Cuts at: 1391 2018 2117 2672 2679 2927 1391 Size: 627 99 555 7 248 4072 Fragments arranged by size: 4072 627 555 248 99 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4637 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2700 2734 3799 3833 1903 Size: 34 50 34 679 34 1065 34 3678 Fragments arranged by size: 3678 1065 679 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3500 4710 3500 Size: 1210 4398 BclI T'GATC_A Cuts at: 1548 1548 Size: 5608 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4219 136 Size: 107 122 71 631 605 2547 1525 Fragments arranged by size: 2547 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2535 5603 2535 Size: 3068 2540 BlpI GC'TnA_GC Cuts at: 2012 3025 2012 Size: 1013 4595 Bme1580I G_kGCm'C Cuts at: 1790 2637 2672 2679 3669 4825 1790 Size: 847 35 7 990 1156 2573 Fragments arranged by size: 2573 1156 990 847 35 7 BmgBI CAC'GTC Cuts at: 1318 2520 1318 Size: 1202 4406 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3339 4297 442 Size: 416 53 83 399 24 1922 958 1753 Fragments arranged by size: 1922 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5608 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2081 2465 4268 1239 Size: 842 384 1803 2579 Fragments arranged by size: 2579 1803 842 384 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3733 4598 4896 3733 Size: 865 298 4445 Fragments arranged by size: 4445 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4271 882 Size: 617 2772 2219 Fragments arranged by size: 2772 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3399 493 Size: 711 2195 2702 Fragments arranged by size: 2702 2195 711 BsaBI GATnn'nnATC Cuts at: 5602 5602 Size: 5608 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3415 3797 4432 275 Size: 53 83 186 936 1882 382 635 1451 Fragments arranged by size: 1882 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4041 4439 4713 4860 5271 4041 Size: 398 274 147 411 4378 Fragments arranged by size: 4378 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5383 Fragments arranged by size: 5383 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5608 BseYI C'CCAG_C Cuts at: 2696 4811 5307 2696 Size: 2115 496 2997 Fragments arranged by size: 2997 2115 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2730 1163 Size: 573 994 4041 Fragments arranged by size: 4041 994 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3058 3819 4847 1540 Size: 275 186 1057 761 1028 2301 Fragments arranged by size: 2301 1057 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2418 2496 2755 3669 3754 4825 2418 Size: 78 259 914 85 1071 3201 Fragments arranged by size: 3201 1071 914 259 85 78 BsmI GAATG_Cn' Cuts at: 2860 3119 3212 2860 Size: 259 93 5256 Fragments arranged by size: 5256 259 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2018 2117 2418 2496 2637 2672 2679 Size: 399 228 99 301 78 141 35 7 Cuts at: 2679 2755 2927 3669 3754 4825 1391 Size: 76 172 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 301 228 172 141 99 85 78 76 35 7 BspEI T'CCGG_A Cuts at: 5271 5271 Size: 5608 BspHI T'CATG_A Cuts at: 3497 5227 3497 Size: 1730 3878 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2422 2518 2695 2786 2963 844 Size: 1578 96 177 91 177 3489 Fragments arranged by size: 3489 1578 177 177 96 91 BsrBI CCG'CTC Cuts at: 1394 1823 2071 2373 2449 3495 1394 Size: 429 248 302 76 1046 3507 Fragments arranged by size: 3507 1046 429 302 248 76 BsrDI GCAATG_nn' Cuts at: 65 1248 4103 4277 65 Size: 1183 2855 174 1396 Fragments arranged by size: 2855 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4252 4439 1700 Size: 110 2442 187 2869 Fragments arranged by size: 2869 2442 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5608 BssHII G'CGCG_C Cuts at: 1925 2505 2790 1925 Size: 580 285 4743 Fragments arranged by size: 4743 580 285 BssSI C'ACGA_G Cuts at: 3668 4680 3668 Size: 1012 4596 BstAPI GCAn_nnn'nTGC Cuts at: 794 2661 794 Size: 1867 3741 BstBI TT'CG_AA Cuts at: 4412 4412 Size: 5608 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5608 BstYI r'GATC_y Cuts at: 1972 2535 2938 3305 3690 3707 5159 5245 5257 Size: 563 403 367 385 17 1452 86 12 Cuts at: 5257 5603 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 563 403 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2082 2099 513 Size: 552 1017 17 4022 Fragments arranged by size: 4022 1017 552 17 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2664 487 Size: 683 56 1438 3431 Fragments arranged by size: 3431 1438 683 56 BtsI GCAGTG_nn' Cuts at: 2721 3124 3918 3938 2721 Size: 403 794 20 4391 Fragments arranged by size: 4391 794 403 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5608 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2161 2196 529 Size: 35 1597 35 3941 Fragments arranged by size: 3941 1597 35 35 DraI TTT'AAA Cuts at: 2912 2912 Size: 5608 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4615 809 Size: 507 3299 1802 Fragments arranged by size: 3299 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2368 2499 2602 Size: 54 1425 50 275 18 538 131 103 Cuts at: 2602 2823 2848 3055 3944 5573 8 Size: 221 25 207 889 1629 43 Fragments arranged by size: 1629 1425 889 538 275 221 207 131 103 54 50 43 25 18 EagI C'GGCC_G Cuts at: 1537 1812 3055 1537 Size: 275 1243 4090 Fragments arranged by size: 4090 1243 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2105 3538 1353 Size: 752 1433 3423 Fragments arranged by size: 3423 1433 752 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4195 4569 4715 387 Size: 1064 138 2606 374 146 1280 Fragments arranged by size: 2606 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2081 2465 3671 4268 5055 5323 1239 Size: 393 449 384 1206 597 787 268 1524 Fragments arranged by size: 1524 1206 787 597 449 393 384 268 EcoO109I rG'GnC_Cy Cuts at: 2228 2669 2801 2901 2228 Size: 441 132 100 4935 Fragments arranged by size: 4935 441 132 100 EcoRI G'AATT_C Cuts at: 2917 3295 2917 Size: 378 5230 FspI TGC'GCA Cuts at: 4114 4114 Size: 5608 HaeII r_GCGC'y Cuts at: 1678 2455 2483 2578 4755 1678 Size: 777 28 95 2177 2531 Fragments arranged by size: 2531 2177 777 95 28 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4249 4281 4323 4355 1373 Size: 32 135 32 2677 32 42 32 2626 Fragments arranged by size: 2677 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2952 3198 669 Size: 988 281 1014 246 3079 Fragments arranged by size: 3079 1014 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5608 HpaI GTT'AAC Cuts at: 3198 3198 Size: 5608 KpnI G_GTAC'C Cuts at: 2473 2933 2473 Size: 460 5148 MfeI C'AATT_G Cuts at: 3207 3207 Size: 5608 MluI A'CGCG_T Cuts at: 5484 5484 Size: 5608 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4722 4906 1305 Size: 3417 184 2007 Fragments arranged by size: 3417 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2501 2604 2825 2850 10 Size: 54 1425 343 669 103 221 25 2768 Fragments arranged by size: 2768 1425 669 343 221 103 54 25 MslI CAynn'nnrTG Cuts at: 518 1166 3566 3925 4084 518 Size: 648 2400 359 159 2042 Fragments arranged by size: 2400 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2084 2101 2349 2467 2748 3024 3701 4849 Size: 478 17 248 118 281 276 677 1148 Cuts at: 4849 5094 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 478 281 276 248 245 118 17 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5056 NdeI CA'TA_TG Cuts at: 387 387 Size: 5608 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5608 NotI GC'GGCC_GC Cuts at: 3055 3055 Size: 5608 NspI r_CATG'y Cuts at: 1132 1229 2966 4511 1132 Size: 97 1737 1545 2229 Fragments arranged by size: 2229 1737 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5608 PciI A'CATG_T Cuts at: 4507 4507 Size: 5608 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5608 PmeI GTTT'AAAC Cuts at: 2912 2912 Size: 5608 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2282 2314 3677 3709 5190 5222 2282 Size: 32 1363 32 1481 32 2668 Fragments arranged by size: 2668 1481 1363 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2228 2801 2228 Size: 573 5035 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5608 PsiI TTA'TAA Cuts at: 3178 3178 Size: 5608 PspOMI G'GGCC_C Cuts at: 2668 2675 2668 Size: 7 5601 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2540 2572 2540 Size: 32 5576 PstI C_TGCA'G Cuts at: 830 2514 2892 2960 830 Size: 1684 378 68 3478 Fragments arranged by size: 3478 1684 378 68 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5608 SacII CC_GC'GG Cuts at: 2085 2102 2085 Size: 17 5591 SalI G'TCGA_C Cuts at: 1936 2950 1936 Size: 1014 4594 SanDI GG'GwC_CC Cuts at: 2228 2228 Size: 5608 SapI GCTCTTCn'nnn_ Cuts at: 2105 2105 Size: 5608 SbfI CC_TGCA'GG Cuts at: 2960 2960 Size: 5608 ScaI AGT'ACT Cuts at: 3856 5568 3856 Size: 1712 3896 SfcI C'TryA_G Cuts at: 826 1045 2510 2704 2743 2888 2956 4091 4772 Size: 219 1465 194 39 145 68 1135 681 Cuts at: 4772 4963 826 Size: 191 1471 Fragments arranged by size: 1471 1465 1135 681 219 194 191 145 68 39 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5608 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5608 SmaI CCC'GGG Cuts at: 1872 2674 2935 1872 Size: 802 261 4545 Fragments arranged by size: 4545 802 261 SmlI C'TyrA_G Cuts at: 820 1017 1943 3712 4613 4875 820 Size: 197 926 1769 901 262 1553 Fragments arranged by size: 1769 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3399 493 Size: 2906 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5608 SphI G_CATG'C Cuts at: 1132 2966 1132 Size: 1834 3774 SspI AAT'ATT Cuts at: 5 52 3532 5 Size: 47 3480 2081 Fragments arranged by size: 3480 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5608 StyI C'CwwG_G Cuts at: 513 1065 2385 3047 513 Size: 552 1320 662 3074 Fragments arranged by size: 3074 1320 662 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2029 3635 3652 3805 3990 4435 325 Size: 1255 449 1606 17 153 185 445 1498 Fragments arranged by size: 1606 1498 1255 449 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3854 5566 96 Size: 274 80 33 51 1443 1877 1712 138 Fragments arranged by size: 1877 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2676 2708 5568 5600 2676 Size: 32 2860 32 2684 Fragments arranged by size: 2860 2684 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5608 XbaI T'CTAG_A Cuts at: 2944 2944 Size: 5608 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5608 XmaI C'CCGG_G Cuts at: 1870 2672 2933 1870 Size: 802 261 4545 Fragments arranged by size: 4545 802 261 XmnI GAAnn'nnTTC Cuts at: 2124 3737 2124 Size: 1613 3995 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3416 276 Size: 53 83 186 936 1882 2468 Fragments arranged by size: 2468 1882 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI SacII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PvuII RsrII SacI SexAI SfiI SfoI SrfI SwaI XcmI