Restriction map for
pFN21AB8941
(Circular) (Six-base) MAPSORT of: pFN21AB8941.seq Check: 1803 from: 1 to: 5608
pFN21AB8941 5608 bp
With 182 enzymes: SgfI *
December 8, 2014 13:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2381 2381
Size: 5608
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3418 278
Size: 53 83 186 936 1882 2468
Fragments arranged by size:
2468 1882 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2864 2951 1937
Size: 927 87 4594
Fragments arranged by size:
4594 927 87
Acc65I G'GTAC_C
Cuts at: 2929 2929
Size: 5608
AclI AA'CG_TT
Cuts at: 1563 3735 4108 1563
Size: 2172 373 3063
Fragments arranged by size:
3063 2172 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2496 3671 5055 5323 1632
Size: 864 1175 1384 268 1917
Fragments arranged by size:
1917 1384 1175 864 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5411
AflIII A'CryG_T
Cuts at: 1203 4507 5484 1203
Size: 3304 977 1327
Fragments arranged by size:
3304 1327 977
AgeI A'CCGG_T
Cuts at: 4439 4439
Size: 5608
AhdI GACnn_n'nnGTC
Cuts at: 4337 4337
Size: 5608
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5608
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5608
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2651 2683 2651
Size: 32 5576
AlwNI CAG_nnn'CTG
Cuts at: 3351 4923 3351
Size: 1572 4036
ApaI G_GGCC'C
Cuts at: 2359 2698 2359
Size: 339 5269
ApaLI G'TGCA_C
Cuts at: 3665 4821 3665
Size: 1156 4452
ApoI r'AATT_y
Cuts at: 1493 2917 3143 3295 5404 1493
Size: 1424 226 152 2109 1697
Fragments arranged by size:
2109 1697 1424 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4162 160
Size: 872 3130 1606
Fragments arranged by size:
3130 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5608
AvaI C'yCGr_G
Cuts at: 1870 1943 2248 2344 2933 1870
Size: 73 305 96 589 4545
Fragments arranged by size:
4545 589 305 96 73
BamHI G'GATC_C
Cuts at: 2938 3305 2938
Size: 367 5241
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2224 2929 4384 618
Size: 325 217 627 437 705 1455 1842
Fragments arranged by size:
1842 1455 705 627 437 325 217
BanII G_rGCy'C
Cuts at: 1391 2359 2609 2698 2730 2927 1391
Size: 968 250 89 32 197 4072
Fragments arranged by size:
4072 968 250 197 89 32
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4637
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3799 3833 1903
Size: 34 1862 34 3678
Fragments arranged by size:
3678 1862 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3500 4710 3500
Size: 1210 4398
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5608
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4219 136
Size: 107 122 71 631 605 2547 1525
Fragments arranged by size:
2547 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5603 5603
Size: 5608
BlpI GC'TnA_GC
Cuts at: 3025 3025
Size: 5608
Bme1580I G_kGCm'C
Cuts at: 1790 2229 2359 2698 3669 4825 1790
Size: 439 130 339 971 1156 2573
Fragments arranged by size:
2573 1156 971 439 339 130
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5608
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2607 3339 4297
Size: 416 53 83 399 24 1190 732 958
Cuts at: 4297 442
Size: 1753
Fragments arranged by size:
1753 1190 958 732 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5608
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2744 2865 4268 1239
Size: 1505 121 1403 2579
Fragments arranged by size:
2579 1505 1403 121
Bpu10I CC'TnA_GC
Cuts at: 2112 2687 2112
Size: 575 5033
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3733 4598 4896 3733
Size: 865 298 4445
Fragments arranged by size:
4445 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4271 882
Size: 617 2772 2219
Fragments arranged by size:
2772 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3399 493
Size: 711 2195 2702
Fragments arranged by size:
2702 2195 711
BsaBI GATnn'nnATC
Cuts at: 5602 5602
Size: 5608
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3415 3797 4432 275
Size: 53 83 186 936 1882 382 635 1451
Fragments arranged by size:
1882 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4041 4439 4713 4860 5271 4041
Size: 398 274 147 411 4378
Fragments arranged by size:
4378 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2790 2820 1178
Size: 30 165 30 1387 30 3966
Fragments arranged by size:
3966 1387 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2471 1186
Size: 1285 4323
BseYI C'CCAG_C
Cuts at: 2060 2199 2234 2276 2358 2812 2891 4811 5307
Size: 139 35 42 82 454 79 1920 496
Cuts at: 5307 2060
Size: 2361
Fragments arranged by size:
2361 1920 496 454 139 82 79 42
35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5035
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3058 3819 4847 1540
Size: 275 186 1057 761 1028 2301
Fragments arranged by size:
2301 1057 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2730 3669 3754 4825 2730
Size: 939 85 1071 3513
Fragments arranged by size:
3513 1071 939 85
BsmI GAATG_Cn'
Cuts at: 3119 3212 3119
Size: 93 5515
BsmBI CGTCTCn'nnnn_
Cuts at: 2316 2316
Size: 5608
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2229 2359 2609 2698 2730 2927 3669
Size: 399 439 130 250 89 32 197 742
Cuts at: 3669 3754 4825 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 439 399 250 197 130
89 85 32
BspEI T'CCGG_A
Cuts at: 5271 5271
Size: 5608
BspHI T'CATG_A
Cuts at: 3497 5227 3497
Size: 1730 3878
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2381 2963 844
Size: 1537 582 3489
Fragments arranged by size:
3489 1537 582
BsrBI CCG'CTC
Cuts at: 1394 1823 3495 1394
Size: 429 1672 3507
Fragments arranged by size:
3507 1672 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4103 4277 65
Size: 1183 2855 174 1396
Fragments arranged by size:
2855 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2399 2464 4252 4439 1700
Size: 110 589 65 1788 187 2869
Fragments arranged by size:
2869 1788 589 187 110 65
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5608
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5608
BssSI C'ACGA_G
Cuts at: 3668 4680 3668
Size: 1012 4596
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5608
BstBI TT'CG_AA
Cuts at: 4412 4412
Size: 5608
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5608
BstYI r'GATC_y
Cuts at: 1972 2938 3305 3690 3707 5159 5245 5257 5603
Size: 966 367 385 17 1452 86 12 346
Cuts at: 5603 1972
Size: 1977
Fragments arranged by size:
1977 1452 966 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2529 513
Size: 552 939 525 3592
Fragments arranged by size:
3592 939 552 525
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4869
Fragments arranged by size:
4869 683 56
BtsI GCAGTG_nn'
Cuts at: 3124 3918 3938 3124
Size: 794 20 4794
Fragments arranged by size:
4794 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5608
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5573
DraI TTT'AAA
Cuts at: 2912 2912
Size: 5608
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4615 809
Size: 507 3299 1802
Fragments arranged by size:
3299 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3055 3944 5573
Size: 54 1425 50 275 18 1225 889 1629
Cuts at: 5573 8
Size: 43
Fragments arranged by size:
1629 1425 1225 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3055 1537
Size: 275 1243 4090
Fragments arranged by size:
4090 1243 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3538 1353
Size: 2185 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4195 4569 4715 387
Size: 1064 138 2606 374 146 1280
Fragments arranged by size:
2606 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2728 2728
Size: 5608
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2496 2744 2865 3671 4268 5055 5323
Size: 393 864 248 121 806 597 787 268
Cuts at: 5323 1239
Size: 1524
Fragments arranged by size:
1524 864 806 787 597 393 268 248
121
EcoNI CCTnn'n_nnAGG
Cuts at: 2486 2486
Size: 5608
EcoO109I rG'GnC_Cy
Cuts at: 2057 2355 2806 2057
Size: 298 451 4859
Fragments arranged by size:
4859 451 298
EcoRI G'AATT_C
Cuts at: 2917 3295 2917
Size: 378 5230
FspI TGC'GCA
Cuts at: 4114 4114
Size: 5608
HaeII r_GCGC'y
Cuts at: 1678 2067 2076 2085 4755 1678
Size: 389 9 9 2670 2531
Fragments arranged by size:
2670 2531 389 9 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2468 2500 2790 2822 4249
Size: 32 135 32 896 32 290 32 1427
Cuts at: 4249 4281 4323 4355 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1427 896 290 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2122 2865 2952 3198 669
Size: 988 281 184 743 87 246 3079
Fragments arranged by size:
3079 988 743 281 246 184 87
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5608
HpaI GTT'AAC
Cuts at: 3198 3198
Size: 5608
KpnI G_GTAC'C
Cuts at: 2933 2933
Size: 5608
MfeI C'AATT_G
Cuts at: 3207 3207
Size: 5608
MluI A'CGCG_T
Cuts at: 5484 5484
Size: 5608
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4722 4906 1305
Size: 3417 184 2007
Fragments arranged by size:
3417 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3786
Fragments arranged by size:
3786 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3566 3925 4084 518
Size: 648 2400 359 159 2042
Fragments arranged by size:
2400 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2647 2843 3024 3701 4849 5094 1606
Size: 1041 196 181 677 1148 245 2120
Fragments arranged by size:
2120 1148 1041 677 245 196 181
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4117
Fragments arranged by size:
4117 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5608
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5608
NotI GC'GGCC_GC
Cuts at: 3055 3055
Size: 5608
NspI r_CATG'y
Cuts at: 1132 1229 2871 2966 4511 1132
Size: 97 1642 95 1545 2229
Fragments arranged by size:
2229 1642 1545 97 95
PasI CC'CwG_GG
Cuts at: 1348 2218 1348
Size: 870 4738
PciI A'CATG_T
Cuts at: 4507 4507
Size: 5608
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5608
PmeI GTTT'AAAC
Cuts at: 2912 2912
Size: 5608
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3677 3709 5190 5222 3677
Size: 32 1481 32 4063
Fragments arranged by size:
4063 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2057 2806 2057
Size: 749 4859
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5608
PsiI TTA'TAA
Cuts at: 3178 3178
Size: 5608
PspOMI G'GGCC_C
Cuts at: 2355 2694 2355
Size: 339 5269
PspXI vC'TCGA_Gb
Cuts at: 2344 2344
Size: 5608
PstI C_TGCA'G
Cuts at: 830 2273 2960 830
Size: 1443 687 3478
Fragments arranged by size:
3478 1443 687
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5608
PvuII CAG'CTG
Cuts at: 2647 2843 2647
Size: 196 5412
RsrII CG'GwC_CG
Cuts at: 2402 2402
Size: 5608
SacI G_AGCT'C
Cuts at: 2730 2730
Size: 5608
SalI G'TCGA_C
Cuts at: 1936 2863 2950 1936
Size: 927 87 4594
Fragments arranged by size:
4594 927 87
SanDI GG'GwC_CC
Cuts at: 2057 2806 2057
Size: 749 4859
SbfI CC_TGCA'GG
Cuts at: 2960 2960
Size: 5608
ScaI AGT'ACT
Cuts at: 2297 3856 5568 2297
Size: 1559 1712 2337
Fragments arranged by size:
2337 1712 1559
SfcI C'TryA_G
Cuts at: 826 1045 2068 2269 2956 4091 4772 4963 826
Size: 219 1023 201 687 1135 681 191 1471
Fragments arranged by size:
1471 1135 1023 687 681 219 201 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5608
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5608
SmaI CCC'GGG
Cuts at: 1872 2935 1872
Size: 1063 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2344 3712 4613 4875 820
Size: 197 926 401 1368 901 262 1553
Fragments arranged by size:
1553 1368 926 901 401 262 197
SnaBI TAC'GTA
Cuts at: 493 3399 493
Size: 2906 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5608
SphI G_CATG'C
Cuts at: 1132 2966 1132
Size: 1834 3774
SspI AAT'ATT
Cuts at: 5 52 3532 5
Size: 47 3480 2081
Fragments arranged by size:
3480 2081 47
StuI AGG'CCT
Cuts at: 1517 2601 1517
Size: 1084 4524
StyI C'CwwG_G
Cuts at: 513 1065 2004 2832 3047 513
Size: 552 939 828 215 3074
Fragments arranged by size:
3074 939 828 552 215
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2587 2905 3635 3652 3805 3990 4435
Size: 1255 1007 318 730 17 153 185 445
Cuts at: 4435 325
Size: 1498
Fragments arranged by size:
1498 1255 1007 730 445 318 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2295 3854 5566
Size: 274 80 33 51 1443 318 1559 1712
Cuts at: 5566 96
Size: 138
Fragments arranged by size:
1712 1559 1443 318 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5568 5600 5568
Size: 32 5576
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5608
XbaI T'CTAG_A
Cuts at: 2944 2944
Size: 5608
XhoI C'TCGA_G
Cuts at: 1943 2344 1943
Size: 401 5207
XmaI C'CCGG_G
Cuts at: 1870 2933 1870
Size: 1063 4545
XmnI GAAnn'nnTTC
Cuts at: 2027 3737 2027
Size: 1710 3898
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3416 276
Size: 53 83 186 936 1882 2468
Fragments arranged by size:
2468 1882 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PsrI SacII SapI SexAI SfiI SfoI
SrfI SwaI XcmI