Restriction map for
pFN21AB8940
(Circular) (Six-base) MAPSORT of: pFN21AB8940.seq Check: 7926 from: 1 to: 5596
pFN21AB8940 5596 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2620 2620
Size: 5596
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3406 278
Size: 53 83 186 936 1870 2468
Fragments arranged by size:
2468 1870 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2939 1937
Size: 1002 4594
Acc65I G'GTAC_C
Cuts at: 2917 2917
Size: 5596
AclI AA'CG_TT
Cuts at: 1563 3723 4096 1563
Size: 2160 373 3063
Fragments arranged by size:
3063 2160 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2152 2720 3659 5043 5311 1632
Size: 520 568 939 1384 268 1917
Fragments arranged by size:
1917 1384 939 568 520 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5399
AflIII A'CryG_T
Cuts at: 1203 4495 5472 1203
Size: 3292 977 1327
Fragments arranged by size:
3292 1327 977
AgeI A'CCGG_T
Cuts at: 4427 4427
Size: 5596
AhdI GACnn_n'nnGTC
Cuts at: 4325 4325
Size: 5596
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5596
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5596
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2540 2572 2540
Size: 32 5564
AlwNI CAG_nnn'CTG
Cuts at: 2272 2539 2575 3339 4911 2272
Size: 267 36 764 1572 2957
Fragments arranged by size:
2957 1572 764 267 36
ApaLI G'TGCA_C
Cuts at: 3653 4809 3653
Size: 1156 4440
ApoI r'AATT_y
Cuts at: 1493 2905 3131 3283 5392 1493
Size: 1412 226 152 2109 1697
Fragments arranged by size:
2109 1697 1412 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4150 160
Size: 872 3118 1606
Fragments arranged by size:
3118 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5596
AvaI C'yCGr_G
Cuts at: 1870 1943 2396 2405 2921 1870
Size: 73 453 9 516 4545
Fragments arranged by size:
4545 516 453 73 9
BamHI G'GATC_C
Cuts at: 2926 3293 2926
Size: 367 5229
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2227 2789 2804 2842 2917
Size: 325 217 627 440 562 15 38 75
Cuts at: 2917 4372 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 562 440 325 217 75
38 15
BanII G_rGCy'C
Cuts at: 1391 2302 2396 2432 2468 2915 1391
Size: 911 94 36 36 447 4072
Fragments arranged by size:
4072 911 447 94 36 36
BbeI G_GCGC'C
Cuts at: 2231 2846 2231
Size: 615 4981
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4625
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3787 3821 1903
Size: 34 1850 34 3678
Fragments arranged by size:
3678 1850 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3488 4698 3488
Size: 1210 4386
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5596
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2096 2455 4207
Size: 107 122 71 631 605 424 359 1752
Cuts at: 4207 136
Size: 1525
Fragments arranged by size:
1752 1525 631 605 424 359 122 107
71
BglII A'GATC_T
Cuts at: 5591 5591
Size: 5596
BlpI GC'TnA_GC
Cuts at: 2462 2612 3013 2462
Size: 150 401 5045
Fragments arranged by size:
5045 401 150
Bme1580I G_kGCm'C
Cuts at: 1790 3657 4813 1790
Size: 1867 1156 2573
Fragments arranged by size:
2573 1867 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5596
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2282 3327 4285
Size: 416 53 83 399 24 865 1045 958
Cuts at: 4285 442
Size: 1753
Fragments arranged by size:
1753 1045 958 865 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5596
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2567 2587 2711 4256 1239
Size: 1328 20 124 1545 2579
Fragments arranged by size:
2579 1545 1328 124 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2342 3721 4586 4884 2342
Size: 1379 865 298 3054
Fragments arranged by size:
3054 1379 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4259 882
Size: 617 2760 2219
Fragments arranged by size:
2760 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3387 493
Size: 711 2183 2702
Fragments arranged by size:
2702 2183 711
BsaBI GATnn'nnATC
Cuts at: 5590 5590
Size: 5596
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2228 2277 2516 2843
Size: 53 83 186 936 695 49 239 327
Cuts at: 2843 3403 3785 4420 275
Size: 560 382 635 1451
Fragments arranged by size:
1451 936 695 635 560 382 327 239
186 83 53 49
BsaWI w'CCGG_w
Cuts at: 4029 4427 4701 4848 5259 4029
Size: 398 274 147 411 4366
Fragments arranged by size:
4366 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2540 2570 1178
Size: 30 165 30 1137 30 4204
Fragments arranged by size:
4204 1137 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2141 2345 1186
Size: 955 204 4437
Fragments arranged by size:
4437 955 204
BseYI C'CCAG_C
Cuts at: 2084 2293 2470 4799 5295 2084
Size: 209 177 2329 496 2385
Fragments arranged by size:
2385 2329 496 209 177
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2264 2461 1163
Size: 573 528 197 4298
Fragments arranged by size:
4298 573 528 197
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2327 2781 3046 3807 4835 1540
Size: 275 186 326 454 265 761 1028 2301
Fragments arranged by size:
2301 1028 761 454 326 275 265 186
BsiHKAI G_wGCw'C
Cuts at: 3657 3742 4813 3657
Size: 85 1071 4440
Fragments arranged by size:
4440 1071 85
BsmI GAATG_Cn'
Cuts at: 3107 3200 3107
Size: 93 5503
BsmBI CGTCTCn'nnnn_
Cuts at: 2618 2618
Size: 5596
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2302 2396 2432 2468 2915 3657 3742
Size: 399 512 94 36 36 447 742 85
Cuts at: 3742 4813 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 512 447 399 94 85
36 36
BspEI T'CCGG_A
Cuts at: 5259 5259
Size: 5596
BspHI T'CATG_A
Cuts at: 3485 5215 3485
Size: 1730 3866
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2620 2951 844
Size: 1776 331 3489
Fragments arranged by size:
3489 1776 331
BsrBI CCG'CTC
Cuts at: 1394 1823 2329 3483 1394
Size: 429 506 1154 3507
Fragments arranged by size:
3507 1154 506 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4091 4265 65
Size: 1183 2843 174 1396
Fragments arranged by size:
2843 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2056 2449 2728 4240 4427 1700
Size: 110 246 393 279 1512 187 2869
Fragments arranged by size:
2869 1512 393 279 246 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5596
BssHII G'CGCG_C
Cuts at: 1925 2590 2824 1925
Size: 665 234 4697
Fragments arranged by size:
4697 665 234
BssSI C'ACGA_G
Cuts at: 3656 4668 3656
Size: 1012 4584
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5596
BstBI TT'CG_AA
Cuts at: 4400 4400
Size: 5596
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5596
BstYI r'GATC_y
Cuts at: 1972 2926 3293 3678 3695 5147 5233 5245 5591
Size: 954 367 385 17 1452 86 12 346
Cuts at: 5591 1972
Size: 1977
Fragments arranged by size:
1977 1452 954 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2094 2100 2112 2321 2417 2510 2781
Size: 552 1029 6 12 209 96 93 271
Cuts at: 2781 2847 513
Size: 66 3262
Fragments arranged by size:
3262 1029 552 271 209 96 93 66
12 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2566 487
Size: 683 56 1340 3517
Fragments arranged by size:
3517 1340 683 56
BtsI GCAGTG_nn'
Cuts at: 3112 3906 3926 3112
Size: 794 20 4782
Fragments arranged by size:
4782 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5596
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5561
DraI TTT'AAA
Cuts at: 2900 2900
Size: 5596
DraIII CAC_nnn'GTG
Cuts at: 2024 2024
Size: 5596
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4603 809
Size: 507 3287 1802
Fragments arranged by size:
3287 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2097 2187 2324
Size: 54 1425 50 275 18 267 90 137
Cuts at: 2324 2332 2451 2706 2778 3043 3932 5561 8
Size: 8 119 255 72 265 889 1629 43
Fragments arranged by size:
1629 1425 889 275 267 265 255 137
119 90 72 54 50 43 18 8
EagI C'GGCC_G
Cuts at: 1537 1812 2324 2778 3043 1537
Size: 275 512 454 265 4090
Fragments arranged by size:
4090 512 454 275 265
EarI CTCTTCn'nnn_
Cuts at: 1353 2320 3526 1353
Size: 967 1206 3423
Fragments arranged by size:
3423 1206 967
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2032 2549 4183 4557 4703 387
Size: 1064 138 443 517 1634 374 146 1280
Fragments arranged by size:
1634 1280 1064 517 443 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2152 2567 2587 2711 2720 3659 4256
Size: 393 520 415 20 124 9 939 597
Cuts at: 4256 5043 5311 1239
Size: 787 268 1524
Fragments arranged by size:
1524 939 787 597 520 415 393 268
124 20 9
EcoO109I rG'GnC_Cy
Cuts at: 2080 2412 2080
Size: 332 5264
EcoRI G'AATT_C
Cuts at: 2905 3283 2905
Size: 378 5218
FspI TGC'GCA
Cuts at: 4102 4102
Size: 5596
HaeII r_GCGC'y
Cuts at: 1678 2091 2183 2223 2231 2546 2846 4743 1678
Size: 413 92 40 8 315 300 1897 2531
Fragments arranged by size:
2531 1897 413 315 300 92 40 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2297 2329 4237 4269 4311
Size: 32 135 32 725 32 1908 32 42
Cuts at: 4311 4343 1373
Size: 32 2626
Fragments arranged by size:
2626 1908 725 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2940 3186 669
Size: 988 281 1002 246 3079
Fragments arranged by size:
3079 1002 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5596
HpaI GTT'AAC
Cuts at: 3186 3186
Size: 5596
KasI G'GCGC_C
Cuts at: 2227 2842 2227
Size: 615 4981
KpnI G_GTAC'C
Cuts at: 2921 2921
Size: 5596
MfeI C'AATT_G
Cuts at: 3195 3195
Size: 5596
MluI A'CGCG_T
Cuts at: 5472 5472
Size: 5596
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4710 4894 1305
Size: 3405 184 2007
Fragments arranged by size:
3405 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3774
Fragments arranged by size:
3774 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3554 3913 4072 518
Size: 648 2388 359 159 2042
Fragments arranged by size:
2388 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2096 2323 2512 2536 2771 2783 3012 3689
Size: 490 227 189 24 235 12 229 677
Cuts at: 3689 4837 5082 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 490 245 235 229 227
189 24 12
NaeI GCC'GGC
Cuts at: 2058 2451 2058
Size: 393 5203
NarI GG'CG_CC
Cuts at: 2228 2843 2228
Size: 615 4981
NcoI C'CATG_G
Cuts at: 513 1065 2112 513
Size: 552 1047 3997
Fragments arranged by size:
3997 1047 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5596
NgoMIV G'CCGG_C
Cuts at: 2056 2449 2056
Size: 393 5203
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5596
NotI GC'GGCC_GC
Cuts at: 2324 2778 3043 2324
Size: 454 265 4877
Fragments arranged by size:
4877 454 265
NspI r_CATG'y
Cuts at: 1132 1229 2884 2954 4499 1132
Size: 97 1655 70 1545 2229
Fragments arranged by size:
2229 1655 1545 97 70
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5596
PciI A'CATG_T
Cuts at: 4495 4495
Size: 5596
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5596
PmeI GTTT'AAAC
Cuts at: 2900 2900
Size: 5596
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2540 2572 3665 3697 5178 5210 2540
Size: 32 1093 32 1481 32 2926
Fragments arranged by size:
2926 1481 1093 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2412 2412
Size: 5596
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5596
PsiI TTA'TAA
Cuts at: 3166 3166
Size: 5596
PstI C_TGCA'G
Cuts at: 830 2948 830
Size: 2118 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5596
PvuII CAG'CTG
Cuts at: 2536 2771 2536
Size: 235 5361
SacII CC_GC'GG
Cuts at: 2097 2324 2513 2784 2097
Size: 227 189 271 4909
Fragments arranged by size:
4909 271 227 189
SalI G'TCGA_C
Cuts at: 1936 2938 1936
Size: 1002 4594
SbfI CC_TGCA'GG
Cuts at: 2948 2948
Size: 5596
ScaI AGT'ACT
Cuts at: 3844 5556 3844
Size: 1712 3884
SfcI C'TryA_G
Cuts at: 826 1045 2772 2875 2944 4079 4760 4951 826
Size: 219 1727 103 69 1135 681 191 1471
Fragments arranged by size:
1727 1471 1135 681 219 191 103 69
SfoI GGC'GCC
Cuts at: 2229 2844 2229
Size: 615 4981
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5596
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5596
SmaI CCC'GGG
Cuts at: 1872 2923 1872
Size: 1051 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2357 3700 4601 4863 820
Size: 197 926 414 1343 901 262 1553
Fragments arranged by size:
1553 1343 926 901 414 262 197
SnaBI TAC'GTA
Cuts at: 493 3387 493
Size: 2894 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5596
SphI G_CATG'C
Cuts at: 1132 2954 1132
Size: 1822 3774
SspI AAT'ATT
Cuts at: 5 52 3520 5
Size: 47 3468 2081
Fragments arranged by size:
3468 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5596
StyI C'CwwG_G
Cuts at: 513 1065 2112 2211 2661 3035 513
Size: 552 1047 99 450 374 3074
Fragments arranged by size:
3074 1047 552 450 374 99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3623 3640 3793 3978 4423 325
Size: 1255 2043 17 153 185 445 1498
Fragments arranged by size:
2043 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3842 5554 96
Size: 274 80 33 51 1443 1865 1712 138
Fragments arranged by size:
1865 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2262 2294 5556 5588 2262
Size: 32 3262 32 2270
Fragments arranged by size:
3262 2270 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5596
XbaI T'CTAG_A
Cuts at: 2932 2932
Size: 5596
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5596
XmaI C'CCGG_G
Cuts at: 1870 2921 1870
Size: 1051 4545
XmnI GAAnn'nnTTC
Cuts at: 3725 3725
Size: 5596
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3404 276
Size: 53 83 186 936 1870 2468
Fragments arranged by size:
2468 1870 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV
FalI FseI FspAI NruI NsiI PacI PfoI PmlI
PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI
SfiI SrfI SwaI XcmI