Restriction map for
pFN21AB8939
(Circular) (Six-base) MAPSORT of: pFN21AB8939.seq Check: 4058 from: 1 to: 5578
pFN21AB8939 5578 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3388 278
Size: 53 83 186 936 1852 2468
Fragments arranged by size:
2468 1852 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2921 1937
Size: 984 4594
Acc65I G'GTAC_C
Cuts at: 2899 2899
Size: 5578
AclI AA'CG_TT
Cuts at: 1563 3705 4078 1563
Size: 2142 373 3063
Fragments arranged by size:
3063 2142 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3641 5025 5293 1632
Size: 2009 1384 268 1917
Fragments arranged by size:
2009 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5381
AflIII A'CryG_T
Cuts at: 1203 4477 5454 1203
Size: 3274 977 1327
Fragments arranged by size:
3274 1327 977
AgeI A'CCGG_T
Cuts at: 4409 4409
Size: 5578
AhdI GACnn_n'nnGTC
Cuts at: 4307 4307
Size: 5578
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5578
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2435 1184
Size: 1251 4327
AlwNI CAG_nnn'CTG
Cuts at: 2260 2391 2514 3321 4893 2260
Size: 131 123 807 1572 2945
Fragments arranged by size:
2945 1572 807 131 123
ApaI G_GGCC'C
Cuts at: 2759 2759
Size: 5578
ApaLI G'TGCA_C
Cuts at: 3635 4791 3635
Size: 1156 4422
ApoI r'AATT_y
Cuts at: 1493 2345 2887 3113 3265 5374 1493
Size: 852 542 226 152 2109 1697
Fragments arranged by size:
2109 1697 852 542 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4132 160
Size: 872 3100 1606
Fragments arranged by size:
3100 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5578
AvaI C'yCGr_G
Cuts at: 1870 1943 2903 1870
Size: 73 960 4545
Fragments arranged by size:
4545 960 73
BamHI G'GATC_C
Cuts at: 2908 3275 2908
Size: 367 5211
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2315 2779 2899 4354 618
Size: 325 217 627 528 464 120 1455 1842
Fragments arranged by size:
1842 1455 627 528 464 325 217 120
BanII G_rGCy'C
Cuts at: 1391 2202 2759 2876 2897 1391
Size: 811 557 117 21 4072
Fragments arranged by size:
4072 811 557 117 21
BbeI G_GCGC'C
Cuts at: 2319 2319
Size: 5578
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2657 928
Size: 971 758 3849
Fragments arranged by size:
3849 971 758
BbvCI CC'TCA_GC
Cuts at: 2702 2702
Size: 5578
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3769 3803 1903
Size: 34 1832 34 3678
Fragments arranged by size:
3678 1832 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3470 4680 3470
Size: 1210 4368
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5578
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2171 2719 4189
Size: 107 122 71 631 605 499 548 1470
Cuts at: 4189 136
Size: 1525
Fragments arranged by size:
1525 1470 631 605 548 499 122 107
71
BglII A'GATC_T
Cuts at: 5573 5573
Size: 5578
BlpI GC'TnA_GC
Cuts at: 2995 2995
Size: 5578
Bme1580I G_kGCm'C
Cuts at: 1790 2759 3639 4795 1790
Size: 969 880 1156 2573
Fragments arranged by size:
2573 1156 969 880
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5578
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3309 4267 442
Size: 416 53 83 399 24 1892 958 1753
Fragments arranged by size:
1892 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5578
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2234 4238 1239
Size: 995 2004 2579
Fragments arranged by size:
2579 2004 995
Bpu10I CC'TnA_GC
Cuts at: 2702 2702
Size: 5578
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2138 2252 3703 4568 4866 2138
Size: 114 1451 865 298 2850
Fragments arranged by size:
2850 1451 865 298 114
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2375 4241 882
Size: 617 876 1866 2219
Fragments arranged by size:
2219 1866 876 617
BsaAI yAC'GTr
Cuts at: 493 1204 3369 493
Size: 711 2165 2702
Fragments arranged by size:
2702 2165 711
BsaBI GATnn'nnATC
Cuts at: 5572 5572
Size: 5578
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2316 2666 3385 3767
Size: 53 83 186 936 783 350 719 382
Cuts at: 3767 4402 275
Size: 635 1451
Fragments arranged by size:
1451 936 783 719 635 382 350 186
83 53
BsaWI w'CCGG_w
Cuts at: 4011 4409 4683 4830 5241 4011
Size: 398 274 147 411 4348
Fragments arranged by size:
4348 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2564 2594 2858 2888 1178
Size: 30 165 30 1161 30 264 30 3868
Fragments arranged by size:
3868 1161 264 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2393 2690 1186
Size: 1207 297 4074
Fragments arranged by size:
4074 1207 297
BseYI C'CCAG_C
Cuts at: 2092 2759 4781 5277 2092
Size: 667 2022 496 2393
Fragments arranged by size:
2393 2022 667 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2717 1163
Size: 573 981 4024
Fragments arranged by size:
4024 981 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2305 2542 2550 3028 3789 4817
Size: 275 186 304 237 8 478 761 1028
Cuts at: 4817 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 478 304 275 237 186
8
BsiHKAI G_wGCw'C
Cuts at: 2130 2154 2202 3639 3724 4795 2130
Size: 24 48 1437 85 1071 2913
Fragments arranged by size:
2913 1437 1071 85 48 24
BsmI GAATG_Cn'
Cuts at: 3089 3182 3089
Size: 93 5485
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2130 2154 2202 2759 2876 2897 3639
Size: 399 340 24 48 557 117 21 742
Cuts at: 3639 3724 4795 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 557 399 340 117 85
48 24 21
BspEI T'CCGG_A
Cuts at: 5241 5241
Size: 5578
BspHI T'CATG_A
Cuts at: 3467 5197 3467
Size: 1730 3848
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2933 844
Size: 2089 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2089 2798 3465 1394
Size: 429 266 709 667 3507
Fragments arranged by size:
3507 709 667 429 266
BsrDI GCAATG_nn'
Cuts at: 65 1248 4073 4247 65
Size: 1183 2825 174 1396
Fragments arranged by size:
2825 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2526 2776 4222 4409 1700
Size: 110 716 250 1446 187 2869
Fragments arranged by size:
2869 1446 716 250 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5578
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5578
BssSI C'ACGA_G
Cuts at: 2861 3638 4650 2861
Size: 777 1012 3789
Fragments arranged by size:
3789 1012 777
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5578
BstBI TT'CG_AA
Cuts at: 4382 4382
Size: 5578
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5578
BstXI CCAn_nnnn'nTGG
Cuts at: 2167 2863 2167
Size: 696 4882
BstYI r'GATC_y
Cuts at: 1972 2208 2908 3275 3660 3677 5129 5215 5227
Size: 236 700 367 385 17 1452 86 12
Cuts at: 5227 5573 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 700 385 367 346 236 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2166 2182 2319 2484 2818 513
Size: 552 1101 16 137 165 334 3273
Fragments arranged by size:
3273 1101 552 334 165 137 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4839
Fragments arranged by size:
4839 683 56
BtsI GCAGTG_nn'
Cuts at: 2693 2772 2804 3094 3888 3908 2693
Size: 79 32 290 794 20 4363
Fragments arranged by size:
4363 794 290 79 32 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5578
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5543
DraI TTT'AAA
Cuts at: 2882 2882
Size: 5578
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4585 809
Size: 507 3269 1802
Fragments arranged by size:
3269 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2302 2547 3025
Size: 54 1425 50 275 18 472 245 478
Cuts at: 3025 3914 5543 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 478 472 275 245 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2302 2547 3025 1537
Size: 275 490 245 478 4090
Fragments arranged by size:
4090 490 478 275 245
EarI CTCTTCn'nnn_
Cuts at: 1353 3508 1353
Size: 2155 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2303 2492 2802 4165 4539 4685
Size: 1064 138 714 189 310 1363 374 146
Cuts at: 4685 387
Size: 1280
Fragments arranged by size:
1363 1280 1064 714 374 310 189 146
138
EcoICRI GAG'CTC
Cuts at: 2200 2200
Size: 5578
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2234 3641 4238 5025 5293 1239
Size: 393 602 1407 597 787 268 1524
Fragments arranged by size:
1524 1407 787 602 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2756 2756
Size: 5578
EcoRI G'AATT_C
Cuts at: 2887 3265 2887
Size: 378 5200
FspI TGC'GCA
Cuts at: 4084 4084
Size: 5578
HaeII r_GCGC'y
Cuts at: 1678 2319 4725 1678
Size: 641 2406 2531
Fragments arranged by size:
2531 2406 641
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2663 2695 2858 2890 4219
Size: 32 135 32 1091 32 163 32 1329
Cuts at: 4219 4251 4293 4325 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1329 1091 163 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2922 3168 669
Size: 988 281 984 246 3079
Fragments arranged by size:
3079 988 984 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5578
HpaI GTT'AAC
Cuts at: 3168 3168
Size: 5578
KasI G'GCGC_C
Cuts at: 2315 2315
Size: 5578
KpnI G_GTAC'C
Cuts at: 2903 2903
Size: 5578
MfeI C'AATT_G
Cuts at: 3177 3177
Size: 5578
MluI A'CGCG_T
Cuts at: 5454 5454
Size: 5578
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4692 4876 1305
Size: 3387 184 2007
Fragments arranged by size:
3387 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3756
Fragments arranged by size:
3756 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2139 2817 3536 3895 4054 518
Size: 648 843 130 678 719 359 159 2042
Fragments arranged by size:
2042 843 719 678 648 359 159 130
MspA1I CmG'CkG
Cuts at: 1606 2184 2260 2353 2605 2616 2994 3671 4819
Size: 578 76 93 252 11 378 677 1148
Cuts at: 4819 5064 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 578 378 252 245 93
76 11
NarI GG'CG_CC
Cuts at: 2316 2316
Size: 5578
NcoI C'CATG_G
Cuts at: 513 1065 2818 513
Size: 552 1753 3273
Fragments arranged by size:
3273 1753 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5578
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5578
NotI GC'GGCC_GC
Cuts at: 3025 3025
Size: 5578
NspI r_CATG'y
Cuts at: 1132 1229 2936 4481 1132
Size: 97 1707 1545 2229
Fragments arranged by size:
2229 1707 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2311 2717 1348
Size: 963 406 4209
Fragments arranged by size:
4209 963 406
PciI A'CATG_T
Cuts at: 4477 4477
Size: 5578
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5578
PfoI T'CCnGG_A
Cuts at: 2211 2211
Size: 5578
PmeI GTTT'AAAC
Cuts at: 2882 2882
Size: 5578
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3647 3679 5160 5192 3647
Size: 32 1481 32 4033
Fragments arranged by size:
4033 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5578
PsiI TTA'TAA
Cuts at: 3148 3148
Size: 5578
PspOMI G'GGCC_C
Cuts at: 2755 2755
Size: 5578
PstI C_TGCA'G
Cuts at: 830 2070 2256 2829 2930 830
Size: 1240 186 573 101 3478
Fragments arranged by size:
3478 1240 573 186 101
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5578
PvuII CAG'CTG
Cuts at: 2260 2260
Size: 5578
SacI G_AGCT'C
Cuts at: 2202 2202
Size: 5578
SacII CC_GC'GG
Cuts at: 2185 2185
Size: 5578
SalI G'TCGA_C
Cuts at: 1936 2920 1936
Size: 984 4594
SbfI CC_TGCA'GG
Cuts at: 2829 2930 2829
Size: 101 5477
ScaI AGT'ACT
Cuts at: 3826 5538 3826
Size: 1712 3866
SfcI C'TryA_G
Cuts at: 826 1045 2066 2252 2825 2926 4061 4742 4933
Size: 219 1021 186 573 101 1135 681 191
Cuts at: 4933 826
Size: 1471
Fragments arranged by size:
1471 1135 1021 681 573 219 191 186
101
SfiI GGCCn_nnn'nGGCC
Cuts at: 2171 2171
Size: 5578
SfoI GGC'GCC
Cuts at: 2317 2317
Size: 5578
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5578
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5578
SmaI CCC'GGG
Cuts at: 1872 2905 1872
Size: 1033 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2153 2267 3682 4583 4845 820
Size: 197 926 210 114 1415 901 262 1553
Fragments arranged by size:
1553 1415 926 901 262 210 197 114
SnaBI TAC'GTA
Cuts at: 493 3369 493
Size: 2876 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5578
SphI G_CATG'C
Cuts at: 1132 2936 1132
Size: 1804 3774
SspI AAT'ATT
Cuts at: 5 52 3502 5
Size: 47 3450 2081
Fragments arranged by size:
3450 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5578
StyI C'CwwG_G
Cuts at: 513 1065 2160 2556 2818 3017 513
Size: 552 1095 396 262 199 3074
Fragments arranged by size:
3074 1095 552 396 262 199
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3605 3622 3775 3960 4405 325
Size: 1255 2025 17 153 185 445 1498
Fragments arranged by size:
2025 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3824 5536 96
Size: 274 80 33 51 1443 1847 1712 138
Fragments arranged by size:
1847 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2666 2698 5538 5570 2666
Size: 32 2840 32 2674
Fragments arranged by size:
2840 2674 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5578
XbaI T'CTAG_A
Cuts at: 2914 2914
Size: 5578
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2167 2167
Size: 5578
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5578
XmaI C'CCGG_G
Cuts at: 1870 2903 1870
Size: 1033 4545
XmnI GAAnn'nnTTC
Cuts at: 3707 3707
Size: 5578
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3386 276
Size: 53 83 186 936 1852 2468
Fragments arranged by size:
2468 1852 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI
PpuMI PspXI PsrI RsrII SanDI SapI SexAI SrfI
SwaI