Restriction map for
pFN21AB8933
(Circular) (Six-base) MAPSORT of: pFN21AB8933.seq Check: 6554 from: 1 to: 5557
pFN21AB8933 5557 bp
With 182 enzymes: SgfI *
March 28, 2012 14:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2139 2139
Size: 5557
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3367 278
Size: 53 83 186 936 1831 2468
Fragments arranged by size:
2468 1831 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2900 1937
Size: 963 4594
Acc65I G'GTAC_C
Cuts at: 2878 2878
Size: 5557
AclI AA'CG_TT
Cuts at: 1563 3684 4057 1563
Size: 2121 373 3063
Fragments arranged by size:
3063 2121 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2288 3620 5004 5272 1632
Size: 656 1332 1384 268 1917
Fragments arranged by size:
1917 1384 1332 656 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5360
AflIII A'CryG_T
Cuts at: 1203 4456 5433 1203
Size: 3253 977 1327
Fragments arranged by size:
3253 1327 977
AgeI A'CCGG_T
Cuts at: 4388 4388
Size: 5557
AhdI GACnn_n'nnGTC
Cuts at: 2851 4286 2851
Size: 1435 4122
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5557
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5557
AlwNI CAG_nnn'CTG
Cuts at: 2260 2391 2514 3300 4872 2260
Size: 131 123 786 1572 2945
Fragments arranged by size:
2945 1572 786 131 123
ApaLI G'TGCA_C
Cuts at: 3614 4770 3614
Size: 1156 4401
ApoI r'AATT_y
Cuts at: 1493 2345 2660 2866 3092 3244 5353 1493
Size: 852 315 206 226 152 2109 1697
Fragments arranged by size:
2109 1697 852 315 226 206 152
AseI AT'TA_AT
Cuts at: 160 1032 4111 160
Size: 872 3079 1606
Fragments arranged by size:
3079 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5557
AvaI C'yCGr_G
Cuts at: 1870 1943 2749 2882 1870
Size: 73 806 133 4545
Fragments arranged by size:
4545 806 133 73
BamHI G'GATC_C
Cuts at: 2887 3254 2887
Size: 367 5190
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2315 2878 4333 618
Size: 325 217 627 528 563 1455 1842
Fragments arranged by size:
1842 1455 627 563 528 325 217
BanII G_rGCy'C
Cuts at: 1391 2202 2735 2876 1391
Size: 811 533 141 4072
Fragments arranged by size:
4072 811 533 141
BbeI G_GCGC'C
Cuts at: 2319 2319
Size: 5557
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4586
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3748 3782 1903
Size: 34 1811 34 3678
Fragments arranged by size:
3678 1811 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3449 4659 3449
Size: 1210 4347
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5557
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2552 4168 136
Size: 107 122 71 631 605 880 1616 1525
Fragments arranged by size:
1616 1525 880 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5552 5552
Size: 5557
BlpI GC'TnA_GC
Cuts at: 2974 2974
Size: 5557
Bme1580I G_kGCm'C
Cuts at: 1790 3618 4774 1790
Size: 1828 1156 2573
Fragments arranged by size:
2573 1828 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5557
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2766 3288 4246
Size: 416 53 83 399 24 1349 522 958
Cuts at: 4246 442
Size: 1753
Fragments arranged by size:
1753 1349 958 522 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5557
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2680 2680
Size: 5557
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2105 2234 2818 4217 1239
Size: 866 129 584 1399 2579
Fragments arranged by size:
2579 1399 866 584 129
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2138 3682 4547 4845 2138
Size: 1544 865 298 2850
Fragments arranged by size:
2850 1544 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2375 2532 4220 882
Size: 617 876 157 1688 2219
Fragments arranged by size:
2219 1688 876 617 157
BsaAI yAC'GTr
Cuts at: 493 1204 2281 3348 493
Size: 711 1077 1067 2702
Fragments arranged by size:
2702 1077 1067 711
BsaBI GATnn'nnATC
Cuts at: 5551 5551
Size: 5557
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2316 3364 3746 4381
Size: 53 83 186 936 783 1048 382 635
Cuts at: 4381 275
Size: 1451
Fragments arranged by size:
1451 1048 936 783 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2526 3990 4388 4662 4809 5220 2526
Size: 1464 398 274 147 411 2863
Fragments arranged by size:
2863 1464 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2570 2576 2600 2606 1178
Size: 30 165 30 1167 6 24 6 4129
Fragments arranged by size:
4129 1167 165 30 30 24 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2393 2578 2581 2607 1186
Size: 1207 185 3 26 4136
Fragments arranged by size:
4136 1207 185 26 3
BseYI C'CCAG_C
Cuts at: 4760 5256 4760
Size: 496 5061
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2117 1163
Size: 573 381 4603
Fragments arranged by size:
4603 573 381
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2305 3007 3768 4796 1540
Size: 275 186 304 702 761 1028 2301
Fragments arranged by size:
2301 1028 761 702 304 275 186
BsiHKAI G_wGCw'C
Cuts at: 2202 2735 3618 3703 4774 2202
Size: 533 883 85 1071 2985
Fragments arranged by size:
2985 1071 883 533 85
BsmI GAATG_Cn'
Cuts at: 3068 3161 3068
Size: 93 5464
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2202 2735 2876 3618 3703 4774 1391
Size: 399 412 533 141 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 533 412 399 141 85
BspEI T'CCGG_A
Cuts at: 5220 5220
Size: 5557
BspHI T'CATG_A
Cuts at: 3446 5176 3446
Size: 1730 3827
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2088 2139 2912 844
Size: 1244 51 773 3489
Fragments arranged by size:
3489 1244 773 51
BsrBI CCG'CTC
Cuts at: 1394 1823 2638 3444 1394
Size: 429 815 806 3507
Fragments arranged by size:
3507 815 806 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4052 4226 65
Size: 1183 2804 174 1396
Fragments arranged by size:
2804 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2486 4201 4388 1700
Size: 110 676 1715 187 2869
Fragments arranged by size:
2869 1715 676 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5557
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5557
BssSI C'ACGA_G
Cuts at: 3617 4629 3617
Size: 1012 4545
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5557
BstBI TT'CG_AA
Cuts at: 4361 4361
Size: 5557
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5557
BstXI CCAn_nnnn'nTGG
Cuts at: 2167 2842 2167
Size: 675 4882
BstYI r'GATC_y
Cuts at: 1972 2208 2887 3254 3639 3656 5108 5194 5206
Size: 236 679 367 385 17 1452 86 12
Cuts at: 5206 5552 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 679 385 367 346 236 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2182 2319 2547 513
Size: 552 1117 137 228 3523
Fragments arranged by size:
3523 1117 552 228 137
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2436 487
Size: 683 56 1210 3608
Fragments arranged by size:
3608 1210 683 56
BtsI GCAGTG_nn'
Cuts at: 2111 2351 3073 3867 3887 2111
Size: 240 722 794 20 3781
Fragments arranged by size:
3781 794 722 240 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5557
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5522
DraI TTT'AAA
Cuts at: 2861 2861
Size: 5557
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4564 809
Size: 507 3248 1802
Fragments arranged by size:
3248 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2302 2475 3004
Size: 54 1425 50 275 18 472 173 529
Cuts at: 3004 3893 5522 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 529 472 275 173 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2302 3004 1537
Size: 275 490 702 4090
Fragments arranged by size:
4090 702 490 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2649 3487 1353
Size: 1296 838 3423
Fragments arranged by size:
3423 1296 838
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2091 4144 4518 4664 387
Size: 1064 138 502 2053 374 146 1280
Fragments arranged by size:
2053 1280 1064 502 374 146 138
EcoICRI GAG'CTC
Cuts at: 2200 2733 2200
Size: 533 5024
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2105 2234 2288 2818 3620 4217 5004
Size: 393 473 129 54 530 802 597 787
Cuts at: 5004 5272 1239
Size: 268 1524
Fragments arranged by size:
1524 802 787 597 530 473 393 268
129 54
EcoNI CCTnn'n_nnAGG
Cuts at: 2622 2622
Size: 5557
EcoRI G'AATT_C
Cuts at: 2660 2866 3244 2660
Size: 206 378 4973
Fragments arranged by size:
4973 378 206
FspI TGC'GCA
Cuts at: 4063 4063
Size: 5557
HaeII r_GCGC'y
Cuts at: 1678 2092 2319 4704 1678
Size: 414 227 2385 2531
Fragments arranged by size:
2531 2385 414 227
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2675 2707 2837 2869 4198
Size: 32 135 32 1103 32 130 32 1329
Cuts at: 4198 4230 4272 4304 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1329 1103 135 130 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2901 3147 669
Size: 988 281 963 246 3079
Fragments arranged by size:
3079 988 963 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5557
HpaI GTT'AAC
Cuts at: 3147 3147
Size: 5557
KasI G'GCGC_C
Cuts at: 2315 2315
Size: 5557
KpnI G_GTAC'C
Cuts at: 2882 2882
Size: 5557
MfeI C'AATT_G
Cuts at: 3156 3156
Size: 5557
MluI A'CGCG_T
Cuts at: 5433 5433
Size: 5557
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2619 4671 4855 1305
Size: 1314 2052 184 2007
Fragments arranged by size:
2052 2007 1314 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3735
Fragments arranged by size:
3735 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3515 3874 4033 518
Size: 648 843 1506 359 159 2042
Fragments arranged by size:
2042 1506 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2184 2549 2973 3650 4798 5043 1606
Size: 578 365 424 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 578 424 365 245
NarI GG'CG_CC
Cuts at: 2316 2316
Size: 5557
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5005
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5557
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5557
NotI GC'GGCC_GC
Cuts at: 3004 3004
Size: 5557
NspI r_CATG'y
Cuts at: 1132 1229 2915 4460 1132
Size: 97 1686 1545 2229
Fragments arranged by size:
2229 1686 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2311 1348
Size: 963 4594
PciI A'CATG_T
Cuts at: 4456 4456
Size: 5557
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5557
PfoI T'CCnGG_A
Cuts at: 2211 2211
Size: 5557
PmeI GTTT'AAAC
Cuts at: 2861 2861
Size: 5557
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2597 2629 3626 3658 5139 5171 2597
Size: 32 997 32 1481 32 2983
Fragments arranged by size:
2983 1481 997 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5557
PsiI TTA'TAA
Cuts at: 3127 3127
Size: 5557
PstI C_TGCA'G
Cuts at: 830 2070 2256 2709 2787 2909 830
Size: 1240 186 453 78 122 3478
Fragments arranged by size:
3478 1240 453 186 122 78
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5557
SacI G_AGCT'C
Cuts at: 2202 2735 2202
Size: 533 5024
SacII CC_GC'GG
Cuts at: 2185 2550 2185
Size: 365 5192
SalI G'TCGA_C
Cuts at: 1936 2899 1936
Size: 963 4594
SbfI CC_TGCA'GG
Cuts at: 2909 2909
Size: 5557
ScaI AGT'ACT
Cuts at: 3805 5517 3805
Size: 1712 3845
SfcI C'TryA_G
Cuts at: 826 1045 2066 2252 2705 2783 2905 4040 4721
Size: 219 1021 186 453 78 122 1135 681
Cuts at: 4721 4912 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1021 681 453 219 191 186
122 78
SfiI GGCCn_nnn'nGGCC
Cuts at: 2552 2552
Size: 5557
SfoI GGC'GCC
Cuts at: 2317 2317
Size: 5557
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5557
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5557
SmaI CCC'GGG
Cuts at: 1872 2751 2884 1872
Size: 879 133 4545
Fragments arranged by size:
4545 879 133
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2153 3661 4562 4824 820
Size: 197 926 210 1508 901 262 1553
Fragments arranged by size:
1553 1508 926 901 262 210 197
SnaBI TAC'GTA
Cuts at: 493 3348 493
Size: 2855 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5557
SphI G_CATG'C
Cuts at: 1132 2915 1132
Size: 1783 3774
SspI AAT'ATT
Cuts at: 5 52 3481 5
Size: 47 3429 2081
Fragments arranged by size:
3429 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5557
StyI C'CwwG_G
Cuts at: 513 1065 2160 2556 2996 513
Size: 552 1095 396 440 3074
Fragments arranged by size:
3074 1095 552 440 396
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2516 2683 3584 3601 3754 3939 4384
Size: 1255 936 167 901 17 153 185 445
Cuts at: 4384 325
Size: 1498
Fragments arranged by size:
1498 1255 936 901 445 185 167 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3803 5515 96
Size: 274 80 33 51 1443 1826 1712 138
Fragments arranged by size:
1826 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5517 5549 5517
Size: 32 5525
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5557
XbaI T'CTAG_A
Cuts at: 2893 2893
Size: 5557
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5557
XmaI C'CCGG_G
Cuts at: 1870 2749 2882 1870
Size: 879 133 4545
Fragments arranged by size:
4545 879 133
XmnI GAAnn'nnTTC
Cuts at: 3686 3686
Size: 5557
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3365 276
Size: 53 83 186 936 1831 2468
Fragments arranged by size:
2468 1831 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI SacI SacII SalI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoO109I EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SanDI
SapI SexAI SrfI SwaI XcmI