Restriction map for
pFN21AB8932
(Circular) (Six-base) MAPSORT of: pFN21AB8932.seq Check: 4198 from: 1 to: 5554
pFN21AB8932 5554 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3364 278
Size: 53 83 186 936 1828 2468
Fragments arranged by size:
2468 1828 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2375 2897 1937
Size: 438 522 4594
Fragments arranged by size:
4594 522 438
Acc65I G'GTAC_C
Cuts at: 2875 2875
Size: 5554
AclI AA'CG_TT
Cuts at: 1563 3681 4054 1563
Size: 2118 373 3063
Fragments arranged by size:
3063 2118 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3617 5001 5269 1632
Size: 1985 1384 268 1917
Fragments arranged by size:
1985 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5357
AflIII A'CryG_T
Cuts at: 1203 2388 4453 5430 1203
Size: 1185 2065 977 1327
Fragments arranged by size:
2065 1327 1185 977
AgeI A'CCGG_T
Cuts at: 4385 4385
Size: 5554
AhdI GACnn_n'nnGTC
Cuts at: 4283 4283
Size: 5554
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5554
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5554
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2480 2512 2480
Size: 32 5522
AlwNI CAG_nnn'CTG
Cuts at: 3297 4869 3297
Size: 1572 3982
ApaI G_GGCC'C
Cuts at: 2301 2301
Size: 5554
ApaLI G'TGCA_C
Cuts at: 3611 4767 3611
Size: 1156 4398
ApoI r'AATT_y
Cuts at: 1493 2863 3089 3241 5350 1493
Size: 1370 226 152 2109 1697
Fragments arranged by size:
2109 1697 1370 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4108 160
Size: 872 3076 1606
Fragments arranged by size:
3076 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5554
AvaI C'yCGr_G
Cuts at: 1870 1943 2772 2879 1870
Size: 73 829 107 4545
Fragments arranged by size:
4545 829 107 73
BamHI G'GATC_C
Cuts at: 2884 3251 2884
Size: 367 5187
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2077 2159 2212 2414 2875
Size: 325 217 627 290 82 53 202 461
Cuts at: 2875 4330 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 461 325 290 217 202
82 53
BanII G_rGCy'C
Cuts at: 1391 2301 2340 2873 1391
Size: 910 39 533 4072
Fragments arranged by size:
4072 910 533 39
BbeI G_GCGC'C
Cuts at: 2081 2163 2418 2081
Size: 82 255 5217
Fragments arranged by size:
5217 255 82
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4583
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3745 3779 1903
Size: 34 1808 34 3678
Fragments arranged by size:
3678 1808 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3446 4656 3446
Size: 1210 4344
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5554
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2296 2836 4165
Size: 107 122 71 631 605 624 540 1329
Cuts at: 4165 136
Size: 1525
Fragments arranged by size:
1525 1329 631 624 605 540 122 107
71
BglII A'GATC_T
Cuts at: 2577 5549 2577
Size: 2972 2582
BlpI GC'TnA_GC
Cuts at: 2268 2971 2268
Size: 703 4851
Bme1580I G_kGCm'C
Cuts at: 1790 2217 2301 3615 4771 1790
Size: 427 84 1314 1156 2573
Fragments arranged by size:
2573 1314 1156 427 84
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5554
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3285 4243 442
Size: 416 53 83 399 24 1868 958 1753
Fragments arranged by size:
1868 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5554
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2195 2351 2507 2803 4214 1239
Size: 956 156 156 296 1411 2579
Fragments arranged by size:
2579 1411 956 296 156 156
Bpu10I CC'TnA_GC
Cuts at: 2673 2798 2673
Size: 125 5429
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3679 4544 4842 3679
Size: 865 298 4391
Fragments arranged by size:
4391 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4217 882
Size: 617 2718 2219
Fragments arranged by size:
2718 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3345 493
Size: 711 2141 2702
Fragments arranged by size:
2702 2141 711
BsaBI GATnn'nnATC
Cuts at: 5548 5548
Size: 5554
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2078 2160 2325 2415
Size: 53 83 186 936 545 82 165 90
Cuts at: 2415 3361 3743 4378 275
Size: 946 382 635 1451
Fragments arranged by size:
1451 946 936 635 545 382 186 165
90 83 82 53
BsaWI w'CCGG_w
Cuts at: 3987 4385 4659 4806 5217 3987
Size: 398 274 147 411 4324
Fragments arranged by size:
4324 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2480 2510 2730 2760 1178
Size: 30 165 30 1077 30 220 30 3972
Fragments arranged by size:
3972 1077 220 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2030 2348 2450 2630 2717 1186
Size: 844 318 102 180 87 4023
Fragments arranged by size:
4023 844 318 180 102 87
BseYI C'CCAG_C
Cuts at: 4757 5253 4757
Size: 496 5058
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4981
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2525 2840 3004 3765 4793 1540
Size: 275 186 524 315 164 761 1028 2301
Fragments arranged by size:
2301 1028 761 524 315 275 186 164
BsiHKAI G_wGCw'C
Cuts at: 3615 3700 4771 3615
Size: 85 1071 4398
Fragments arranged by size:
4398 1071 85
BsiWI C'GTAC_G
Cuts at: 2205 2205
Size: 5554
BsmI GAATG_Cn'
Cuts at: 3065 3158 3065
Size: 93 5461
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2217 2301 2340 2873 3615 3700 4771
Size: 399 427 84 39 533 742 85 1071
Cuts at: 4771 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 533 427 399 85 84
39
BspEI T'CCGG_A
Cuts at: 5217 5217
Size: 5554
BspHI T'CATG_A
Cuts at: 3443 5173 3443
Size: 1730 3824
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2909 844
Size: 2065 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3441 1394
Size: 429 1618 3507
Fragments arranged by size:
3507 1618 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4049 4223 65
Size: 1183 2801 174 1396
Fragments arranged by size:
2801 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4198 4385 1700
Size: 110 2388 187 2869
Fragments arranged by size:
2869 2388 187 110
BsrGI T'GTAC_A
Cuts at: 96 2112 96
Size: 2016 3538
BssHII G'CGCG_C
Cuts at: 1925 2169 2467 1925
Size: 244 298 5012
Fragments arranged by size:
5012 298 244
BssSI C'ACGA_G
Cuts at: 2850 3614 4626 2850
Size: 764 1012 3778
Fragments arranged by size:
3778 1012 764
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5554
BstBI TT'CG_AA
Cuts at: 4358 4358
Size: 5554
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5554
BstYI r'GATC_y
Cuts at: 1972 2577 2884 3251 3636 3653 5105 5191 5203
Size: 605 307 367 385 17 1452 86 12
Cuts at: 5203 5549 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 605 385 367 346 307 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2235 2363 513
Size: 552 1170 128 3704
Fragments arranged by size:
3704 1170 552 128
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4815
Fragments arranged by size:
4815 683 56
BtsI GCAGTG_nn'
Cuts at: 3070 3864 3884 3070
Size: 794 20 4740
Fragments arranged by size:
4740 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5554
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5519
DraI TTT'AAA
Cuts at: 2858 2858
Size: 5554
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4561 809
Size: 507 3245 1802
Fragments arranged by size:
3245 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2031 2522 2837
Size: 54 1425 50 275 18 201 491 315
Cuts at: 2837 3001 3890 5519 8
Size: 164 889 1629 43
Fragments arranged by size:
1629 1425 889 491 315 275 201 164
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2522 2837 3001 1537
Size: 275 710 315 164 4090
Fragments arranged by size:
4090 710 315 275 164
EarI CTCTTCn'nnn_
Cuts at: 1353 3484 1353
Size: 2131 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4141 4515 4661 387
Size: 1064 138 2552 374 146 1280
Fragments arranged by size:
2552 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2195 2351 2507 2803 3617 4214 5001
Size: 393 563 156 156 296 814 597 787
Cuts at: 5001 5269 1239
Size: 268 1524
Fragments arranged by size:
1524 814 787 597 563 393 296 268
156 156
EcoO109I rG'GnC_Cy
Cuts at: 2050 2298 2050
Size: 248 5306
EcoRI G'AATT_C
Cuts at: 2863 3241 2863
Size: 378 5176
FspI TGC'GCA
Cuts at: 4060 4060
Size: 5554
HaeII r_GCGC'y
Cuts at: 1678 2081 2094 2163 2418 2717 4701 1678
Size: 403 13 69 255 299 1984 2531
Fragments arranged by size:
2531 1984 403 299 255 69 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4195 4227 4269 4301 1373
Size: 32 135 32 2623 32 42 32 2626
Fragments arranged by size:
2626 2623 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2898 3144 669
Size: 988 281 960 246 3079
Fragments arranged by size:
3079 988 960 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5554
HpaI GTT'AAC
Cuts at: 3144 3144
Size: 5554
KasI G'GCGC_C
Cuts at: 2077 2159 2414 2077
Size: 82 255 5217
Fragments arranged by size:
5217 255 82
KpnI G_GTAC'C
Cuts at: 2879 2879
Size: 5554
MfeI C'AATT_G
Cuts at: 3153 3153
Size: 5554
MluI A'CGCG_T
Cuts at: 2388 5430 2388
Size: 3042 2512
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4668 4852 1305
Size: 3363 184 2007
Fragments arranged by size:
3363 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3732
Fragments arranged by size:
3732 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3512 3871 4030 518
Size: 648 2346 359 159 2042
Fragments arranged by size:
2346 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2237 2353 2476 2645 2756 2970 3647 4795
Size: 631 116 123 169 111 214 677 1148
Cuts at: 4795 5040 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 631 245 214 169 123
116 111
NarI GG'CG_CC
Cuts at: 2078 2160 2415 2078
Size: 82 255 5217
Fragments arranged by size:
5217 255 82
NcoI C'CATG_G
Cuts at: 513 1065 2363 513
Size: 552 1298 3704
Fragments arranged by size:
3704 1298 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5554
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5554
NotI GC'GGCC_GC
Cuts at: 2837 3001 2837
Size: 164 5390
NspI r_CATG'y
Cuts at: 1132 1229 2912 4457 1132
Size: 97 1683 1545 2229
Fragments arranged by size:
2229 1683 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2164 1348
Size: 816 4738
PciI A'CATG_T
Cuts at: 4453 4453
Size: 5554
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5554
PfoI T'CCnGG_A
Cuts at: 2304 2328 2304
Size: 24 5530
PmeI GTTT'AAAC
Cuts at: 2858 2858
Size: 5554
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2480 2512 3623 3655 5136 5168 2480
Size: 32 1111 32 1481 32 2866
Fragments arranged by size:
2866 1481 1111 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2050 2050
Size: 5554
PshAI GACnn'nnGTC
Cuts at: 1613 2645 1613
Size: 1032 4522
PsiI TTA'TAA
Cuts at: 3124 3124
Size: 5554
PspOMI G'GGCC_C
Cuts at: 2297 2297
Size: 5554
PstI C_TGCA'G
Cuts at: 830 2906 830
Size: 2076 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5554
PvuII CAG'CTG
Cuts at: 2353 2476 2645 2353
Size: 123 169 5262
Fragments arranged by size:
5262 169 123
SacII CC_GC'GG
Cuts at: 2238 2238
Size: 5554
SalI G'TCGA_C
Cuts at: 1936 2896 1936
Size: 960 4594
SbfI CC_TGCA'GG
Cuts at: 2906 2906
Size: 5554
ScaI AGT'ACT
Cuts at: 2024 3802 5514 2024
Size: 1778 1712 2064
Fragments arranged by size:
2064 1778 1712
SfcI C'TryA_G
Cuts at: 826 1045 2902 4037 4718 4909 826
Size: 219 1857 1135 681 191 1471
Fragments arranged by size:
1857 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2079 2161 2416 2079
Size: 82 255 5217
Fragments arranged by size:
5217 255 82
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5554
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5554
SmaI CCC'GGG
Cuts at: 1872 2881 1872
Size: 1009 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3658 4559 4821 820
Size: 197 926 1715 901 262 1553
Fragments arranged by size:
1715 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3345 493
Size: 2852 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5554
SphI G_CATG'C
Cuts at: 1132 2912 1132
Size: 1780 3774
SspI AAT'ATT
Cuts at: 5 52 3478 5
Size: 47 3426 2081
Fragments arranged by size:
3426 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5554
StyI C'CwwG_G
Cuts at: 513 1065 2363 2993 513
Size: 552 1298 630 3074
Fragments arranged by size:
3074 1298 630 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2576 3581 3598 3751 3936 4381 325
Size: 1255 996 1005 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1005 996 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2022 2112 2511
Size: 274 80 33 51 1443 45 90 399
Cuts at: 2511 3800 5512 96
Size: 1289 1712 138
Fragments arranged by size:
1712 1443 1289 399 274 138 90 80
51 45 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2730 2762 5514 5546 2730
Size: 32 2752 32 2738
Fragments arranged by size:
2752 2738 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5554
XbaI T'CTAG_A
Cuts at: 2890 2890
Size: 5554
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5554
XmaI C'CCGG_G
Cuts at: 1870 2879 1870
Size: 1009 4545
XmnI GAAnn'nnTTC
Cuts at: 2495 3683 2495
Size: 1188 4366
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3362 276
Size: 53 83 186 936 1828 2468
Fragments arranged by size:
2468 1828 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbvCI BfrBI BplI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI RsrII SacI SanDI SapI SexAI
SfiI SrfI SwaI XcmI