Restriction map for
pFN21AB8931
(Circular) (Six-base) MAPSORT of: pFN21AB8931.seq Check: 3095 from: 1 to: 5551
pFN21AB8931 5551 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3361 278
Size: 53 83 186 936 1825 2468
Fragments arranged by size:
2468 1825 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2894 1937
Size: 957 4594
Acc65I G'GTAC_C
Cuts at: 2625 2872 2625
Size: 247 5304
AclI AA'CG_TT
Cuts at: 1563 3678 4051 1563
Size: 2115 373 3063
Fragments arranged by size:
3063 2115 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2345 3614 4998 5266 1632
Size: 713 1269 1384 268 1917
Fragments arranged by size:
1917 1384 1269 713 268
AfeI AGC'GCT
Cuts at: 2373 2373
Size: 5551
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5354
AflIII A'CryG_T
Cuts at: 1203 2151 4450 5427 1203
Size: 948 2299 977 1327
Fragments arranged by size:
2299 1327 977 948
AgeI A'CCGG_T
Cuts at: 4382 4382
Size: 5551
AhdI GACnn_n'nnGTC
Cuts at: 4280 4280
Size: 5551
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5551
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5551
AlwNI CAG_nnn'CTG
Cuts at: 2500 3294 4866 2500
Size: 794 1572 3185
Fragments arranged by size:
3185 1572 794
ApaI G_GGCC'C
Cuts at: 2364 2364
Size: 5551
ApaLI G'TGCA_C
Cuts at: 3608 4764 3608
Size: 1156 4395
ApoI r'AATT_y
Cuts at: 1493 2860 3086 3238 5347 1493
Size: 1367 226 152 2109 1697
Fragments arranged by size:
2109 1697 1367 226 152
AscI GG'CGCG_CC
Cuts at: 2616 2616
Size: 5551
AseI AT'TA_AT
Cuts at: 160 1032 4105 160
Size: 872 3073 1606
Fragments arranged by size:
3073 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5551
AvaI C'yCGr_G
Cuts at: 1870 1943 2030 2114 2350 2363 2876 1870
Size: 73 87 84 236 13 513 4545
Fragments arranged by size:
4545 513 236 87 84 73 13
BamHI G'GATC_C
Cuts at: 2881 3248 2881
Size: 367 5184
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2546 2625 2872 4327 618
Size: 325 217 627 759 79 247 1455 1842
Fragments arranged by size:
1842 1455 759 627 325 247 217 79
BanII G_rGCy'C
Cuts at: 1391 2364 2604 2870 1391
Size: 973 240 266 4072
Fragments arranged by size:
4072 973 266 240
BbeI G_GCGC'C
Cuts at: 2550 2550
Size: 5551
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4580
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3742 3776 1903
Size: 34 1805 34 3678
Fragments arranged by size:
3678 1805 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3443 4653 3443
Size: 1210 4341
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5551
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2395 4162 136
Size: 107 122 71 631 605 723 1767 1525
Fragments arranged by size:
1767 1525 723 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2781 5546 2781
Size: 2765 2786
BlpI GC'TnA_GC
Cuts at: 2968 2968
Size: 5551
Bme1580I G_kGCm'C
Cuts at: 1790 2364 3612 4768 1790
Size: 574 1248 1156 2573
Fragments arranged by size:
2573 1248 1156 574
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5551
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2213 3282 4240
Size: 416 53 83 399 24 796 1069 958
Cuts at: 4240 442
Size: 1753
Fragments arranged by size:
1753 1069 958 796 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5551
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2405 2497 2711 4211 1239
Size: 1166 92 214 1500 2579
Fragments arranged by size:
2579 1500 1166 214 92
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3676 4541 4839 3676
Size: 865 298 4388
Fragments arranged by size:
4388 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4214 882
Size: 617 2715 2219
Fragments arranged by size:
2715 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2250 3342 493
Size: 711 1046 1092 2702
Fragments arranged by size:
2702 1092 1046 711
BsaBI GATnn'nnATC
Cuts at: 5545 5545
Size: 5551
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2547 3358 3740 4375
Size: 53 83 186 936 1014 811 382 635
Cuts at: 4375 275
Size: 1451
Fragments arranged by size:
1451 1014 936 811 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2796 3984 4382 4656 4803 5214 2796
Size: 1188 398 274 147 411 3133
Fragments arranged by size:
3133 1188 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2141 2171 2471 2501 2684
Size: 30 165 30 738 30 300 30 183
Cuts at: 2684 2714 1178
Size: 30 4015
Fragments arranged by size:
4015 738 300 183 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2348 1186
Size: 1162 4389
BseYI C'CCAG_C
Cuts at: 4754 5250 4754
Size: 496 5055
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4978
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2245 3001 3762 4790 1540
Size: 275 186 244 756 761 1028 2301
Fragments arranged by size:
2301 1028 761 756 275 244 186
BsiHKAI G_wGCw'C
Cuts at: 2159 3612 3697 4768 2159
Size: 1453 85 1071 2942
Fragments arranged by size:
2942 1453 1071 85
BsmI GAATG_Cn'
Cuts at: 3062 3155 3062
Size: 93 5458
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2159 2364 2604 2870 3612 3697 4768
Size: 399 369 205 240 266 742 85 1071
Cuts at: 4768 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 369 266 240 205
85
BspEI T'CCGG_A
Cuts at: 5214 5214
Size: 5551
BspHI T'CATG_A
Cuts at: 3440 5170 3440
Size: 1730 3821
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2764 2906 844
Size: 1920 142 3489
Fragments arranged by size:
3489 1920 142
BsrBI CCG'CTC
Cuts at: 1394 1823 2643 3438 1394
Size: 429 820 795 3507
Fragments arranged by size:
3507 820 795 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2194 4046 4220 65
Size: 1183 946 1852 174 1396
Fragments arranged by size:
1852 1396 1183 946 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2549 4195 4382 1700
Size: 110 739 1646 187 2869
Fragments arranged by size:
2869 1646 739 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5551
BssHII G'CGCG_C
Cuts at: 1925 2261 2616 1925
Size: 336 355 4860
Fragments arranged by size:
4860 355 336
BssSI C'ACGA_G
Cuts at: 3611 4623 3611
Size: 1012 4539
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5551
BstBI TT'CG_AA
Cuts at: 2669 4355 2669
Size: 1686 3865
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5551
BstYI r'GATC_y
Cuts at: 1972 2781 2881 3248 3633 3650 5102 5188 5200
Size: 809 100 367 385 17 1452 86 12
Cuts at: 5200 5546 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 809 385 367 346 100 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4060
Fragments arranged by size:
4060 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4812
Fragments arranged by size:
4812 683 56
BtsI GCAGTG_nn'
Cuts at: 3067 3861 3881 3067
Size: 794 20 4737
Fragments arranged by size:
4737 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5551
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5516
DraI TTT'AAA
Cuts at: 2855 2855
Size: 5551
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2774 4558 809
Size: 507 1458 1784 1802
Fragments arranged by size:
1802 1784 1458 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2168 2242 2607
Size: 54 1425 50 275 18 338 74 365
Cuts at: 2607 2998 3887 5516 8
Size: 391 889 1629 43
Fragments arranged by size:
1629 1425 889 391 365 338 275 74
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2242 2998 1537
Size: 275 430 756 4090
Fragments arranged by size:
4090 756 430 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3481 1353
Size: 2128 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2192 4138 4512 4658 387
Size: 1064 138 603 1946 374 146 1280
Fragments arranged by size:
1946 1280 1064 603 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2345 2405 2497 2711 3614 4211 4998
Size: 393 713 60 92 214 903 597 787
Cuts at: 4998 5266 1239
Size: 268 1524
Fragments arranged by size:
1524 903 787 713 597 393 268 214
92 60
EcoO109I rG'GnC_Cy
Cuts at: 2320 2468 2320
Size: 148 5403
EcoRI G'AATT_C
Cuts at: 2860 3238 2860
Size: 378 5173
FspI TGC'GCA
Cuts at: 4057 4057
Size: 5551
HaeII r_GCGC'y
Cuts at: 1678 2375 2550 2734 4698 1678
Size: 697 175 184 1964 2531
Fragments arranged by size:
2531 1964 697 184 175
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2471 2503 4192 4224 4266
Size: 32 135 32 899 32 1689 32 42
Cuts at: 4266 4298 1373
Size: 32 2626
Fragments arranged by size:
2626 1689 899 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2710 2895 3141 669
Size: 988 281 772 185 246 3079
Fragments arranged by size:
3079 988 772 281 246 185
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5551
HpaI GTT'AAC
Cuts at: 3141 3141
Size: 5551
KasI G'GCGC_C
Cuts at: 2546 2546
Size: 5551
KpnI G_GTAC'C
Cuts at: 2629 2876 2629
Size: 247 5304
MfeI C'AATT_G
Cuts at: 3150 3150
Size: 5551
MluI A'CGCG_T
Cuts at: 5427 5427
Size: 5551
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2532 4665 4849 1305
Size: 1227 2133 184 2007
Fragments arranged by size:
2133 2007 1227 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3729
Fragments arranged by size:
3729 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3509 3868 4027 518
Size: 648 2343 359 159 2042
Fragments arranged by size:
2343 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2047 2383 2613 2967 3644 4792 5037 1606
Size: 441 336 230 354 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 441 354 336 245 230
NaeI GCC'GGC
Cuts at: 2551 2551
Size: 5551
NarI GG'CG_CC
Cuts at: 2547 2547
Size: 5551
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4060
Fragments arranged by size:
4060 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5551
NgoMIV G'CCGG_C
Cuts at: 2549 2549
Size: 5551
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5551
NotI GC'GGCC_GC
Cuts at: 2998 2998
Size: 5551
NspI r_CATG'y
Cuts at: 1132 1229 2909 4454 1132
Size: 97 1680 1545 2229
Fragments arranged by size:
2229 1680 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5551
PciI A'CATG_T
Cuts at: 4450 4450
Size: 5551
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2500 1108
Size: 1392 4159
PmeI GTTT'AAAC
Cuts at: 2855 2855
Size: 5551
PmlI CAC'GTG
Cuts at: 2250 2250
Size: 5551
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3620 3652 5133 5165 3620
Size: 32 1481 32 4006
Fragments arranged by size:
4006 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2468 2468
Size: 5551
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5551
PsiI TTA'TAA
Cuts at: 3121 3121
Size: 5551
PspOMI G'GGCC_C
Cuts at: 2360 2360
Size: 5551
PstI C_TGCA'G
Cuts at: 830 2903 830
Size: 2073 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5551
SalI G'TCGA_C
Cuts at: 1936 2893 1936
Size: 957 4594
SbfI CC_TGCA'GG
Cuts at: 2903 2903
Size: 5551
ScaI AGT'ACT
Cuts at: 3799 5511 3799
Size: 1712 3839
SfcI C'TryA_G
Cuts at: 826 1045 2899 4034 4715 4906 826
Size: 219 1854 1135 681 191 1471
Fragments arranged by size:
1854 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2548 2548
Size: 5551
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5551
SgrAI Cr'CCGG_yG
Cuts at: 1700 2549 1700
Size: 849 4702
SmaI CCC'GGG
Cuts at: 1872 2032 2116 2352 2365 2878 1872
Size: 160 84 236 13 513 4545
Fragments arranged by size:
4545 513 236 160 84 13
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3655 4556 4818 820
Size: 197 926 1712 901 262 1553
Fragments arranged by size:
1712 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3342 493
Size: 2849 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5551
SphI G_CATG'C
Cuts at: 1132 2909 1132
Size: 1777 3774
SspI AAT'ATT
Cuts at: 5 52 3475 5
Size: 47 3423 2081
Fragments arranged by size:
3423 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5551
StyI C'CwwG_G
Cuts at: 513 1065 2004 2990 513
Size: 552 939 986 3074
Fragments arranged by size:
3074 986 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3578 3595 3748 3933 4378 325
Size: 1255 1998 17 153 185 445 1498
Fragments arranged by size:
1998 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3797 5509 96
Size: 274 80 33 51 1443 1820 1712 138
Fragments arranged by size:
1820 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5511 5543 5511
Size: 32 5519
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5551
XbaI T'CTAG_A
Cuts at: 2887 2887
Size: 5551
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5551
XmaI C'CCGG_G
Cuts at: 1870 2030 2114 2350 2363 2876 1870
Size: 160 84 236 13 513 4545
Fragments arranged by size:
4545 513 236 160 84 13
XmnI GAAnn'nnTTC
Cuts at: 3680 3680
Size: 5551
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3359 276
Size: 53 83 186 936 1825 2468
Fragments arranged by size:
2468 1825 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AvrII BaeI BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI NruI NsiI PacI PfoI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SrfI SwaI XcmI