Restriction map for
pFN21AB8927
(Circular) (Six-base) MAPSORT of: pFN21AB8927.seq Check: 1813 from: 1 to: 5524
pFN21AB8927 5524 bp
With 182 enzymes: SgfI *
March 28, 2012 12:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3334 278
Size: 53 83 186 936 1798 2468
Fragments arranged by size:
2468 1798 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2867 1937
Size: 930 4594
Acc65I G'GTAC_C
Cuts at: 2273 2845 2273
Size: 572 4952
AclI AA'CG_TT
Cuts at: 1563 3651 4024 1563
Size: 2088 373 3063
Fragments arranged by size:
3063 2088 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3587 4971 5239 1632
Size: 1955 1384 268 1917
Fragments arranged by size:
1955 1917 1384 268
AfeI AGC'GCT
Cuts at: 2519 2519
Size: 5524
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5327
AflIII A'CryG_T
Cuts at: 1203 2661 4423 5400 1203
Size: 1458 1762 977 1327
Fragments arranged by size:
1762 1458 1327 977
AgeI A'CCGG_T
Cuts at: 4355 4355
Size: 5524
AhdI GACnn_n'nnGTC
Cuts at: 4253 4253
Size: 5524
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5524
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5524
AlwNI CAG_nnn'CTG
Cuts at: 2677 3267 4839 2677
Size: 590 1572 3362
Fragments arranged by size:
3362 1572 590
ApaI G_GGCC'C
Cuts at: 2093 2237 2303 2093
Size: 144 66 5314
Fragments arranged by size:
5314 144 66
ApaLI G'TGCA_C
Cuts at: 3581 4737 3581
Size: 1156 4368
ApoI r'AATT_y
Cuts at: 1493 2833 3059 3211 5320 1493
Size: 1340 226 152 2109 1697
Fragments arranged by size:
2109 1697 1340 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4078 160
Size: 872 3046 1606
Fragments arranged by size:
3046 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5524
AvaI C'yCGr_G
Cuts at: 1870 1943 2296 2302 2495 2767 2849 1870
Size: 73 353 6 193 272 82 4545
Fragments arranged by size:
4545 353 272 193 82 73 6
BamHI G'GATC_C
Cuts at: 2854 3221 2854
Size: 367 5157
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2101 2188 2273 2728 2845
Size: 325 217 627 314 87 85 455 117
Cuts at: 2845 4300 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 455 325 314 217 117
87 85
BanII G_rGCy'C
Cuts at: 1391 2093 2115 2237 2303 2387 2843 1391
Size: 702 22 122 66 84 456 4072
Fragments arranged by size:
4072 702 456 122 84 66 22
BbeI G_GCGC'C
Cuts at: 2192 2192
Size: 5524
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2462 928
Size: 971 563 3990
Fragments arranged by size:
3990 971 563
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3715 3749 1903
Size: 34 1778 34 3678
Fragments arranged by size:
3678 1778 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3416 4626 3416
Size: 1210 4314
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5524
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2307 2444 2654
Size: 107 122 71 631 605 635 137 210
Cuts at: 2654 4135 136
Size: 1481 1525
Fragments arranged by size:
1525 1481 635 631 605 210 137 122
107 71
BglII A'GATC_T
Cuts at: 5519 5519
Size: 5524
BlpI GC'TnA_GC
Cuts at: 2427 2634 2941 2427
Size: 207 307 5010
Fragments arranged by size:
5010 307 207
Bme1580I G_kGCm'C
Cuts at: 1790 2093 2237 2303 3585 4741 1790
Size: 303 144 66 1282 1156 2573
Fragments arranged by size:
2573 1282 1156 303 144 66
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5524
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3255 4213 442
Size: 416 53 83 399 24 1838 958 1753
Fragments arranged by size:
1838 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5524
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2041 2539 2660 4184 1239
Size: 802 498 121 1524 2579
Fragments arranged by size:
2579 1524 802 498 121
Bpu10I CC'TnA_GC
Cuts at: 2525 2525
Size: 5524
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3649 4514 4812 3649
Size: 865 298 4361
Fragments arranged by size:
4361 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2567 4187 882
Size: 617 1068 1620 2219
Fragments arranged by size:
2219 1620 1068 617
BsaAI yAC'GTr
Cuts at: 493 1204 3315 493
Size: 711 2111 2702
Fragments arranged by size:
2702 2111 711
BsaBI GATnn'nnATC
Cuts at: 5518 5518
Size: 5524
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2052 2189 3331 3713
Size: 53 83 186 936 519 137 1142 382
Cuts at: 3713 4348 275
Size: 635 1451
Fragments arranged by size:
1451 1142 936 635 519 382 186 137
83 53
BsaWI w'CCGG_w
Cuts at: 3957 4355 4629 4776 5187 3957
Size: 398 274 147 411 4294
Fragments arranged by size:
4294 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5299
Fragments arranged by size:
5299 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2174 2177 2180 1186
Size: 988 3 3 4530
Fragments arranged by size:
4530 988 3 3
BseYI C'CCAG_C
Cuts at: 4727 5223 4727
Size: 496 5028
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4951
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2046 2411 2974 3735 4763 1540
Size: 275 186 45 365 563 761 1028 2301
Fragments arranged by size:
2301 1028 761 563 365 275 186 45
BsiHKAI G_wGCw'C
Cuts at: 3585 3670 4741 3585
Size: 85 1071 4368
Fragments arranged by size:
4368 1071 85
BsmI GAATG_Cn'
Cuts at: 3035 3128 3035
Size: 93 5431
BsmBI CGTCTCn'nnnn_
Cuts at: 2059 2059
Size: 5524
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2093 2115 2237 2303 2387 2843 3585
Size: 399 303 22 122 66 84 456 742
Cuts at: 3585 3670 4741 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 456 399 303 122 85
84 66 22
BspEI T'CCGG_A
Cuts at: 5187 5187
Size: 5524
BspHI T'CATG_A
Cuts at: 3413 5143 3413
Size: 1730 3794
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2879 844
Size: 2035 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2407 2431 2758 3411 1394
Size: 429 584 24 327 653 3507
Fragments arranged by size:
3507 653 584 429 327 24
BsrDI GCAATG_nn'
Cuts at: 65 1248 4019 4193 65
Size: 1183 2771 174 1396
Fragments arranged by size:
2771 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2045 2438 2725 4168 4355 1700
Size: 110 235 393 287 1443 187 2869
Fragments arranged by size:
2869 1443 393 287 235 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5524
BssHII G'CGCG_C
Cuts at: 1925 2539 1925
Size: 614 4910
BssSI C'ACGA_G
Cuts at: 3584 4596 3584
Size: 1012 4512
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5524
BstBI TT'CG_AA
Cuts at: 4328 4328
Size: 5524
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5524
BstYI r'GATC_y
Cuts at: 1972 2854 3221 3606 3623 5075 5161 5173 5519
Size: 882 367 385 17 1452 86 12 346
Cuts at: 5519 1972
Size: 1977
Fragments arranged by size:
1977 1452 882 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2223 2223
Size: 5524
BtgI C'CryG_G
Cuts at: 513 1065 2004 2105 2379 2411 513
Size: 552 939 101 274 32 3626
Fragments arranged by size:
3626 939 552 274 101 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4785
Fragments arranged by size:
4785 683 56
BtsI GCAGTG_nn'
Cuts at: 3040 3834 3854 3040
Size: 794 20 4710
Fragments arranged by size:
4710 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5524
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5489
DraI TTT'AAA
Cuts at: 2353 2828 2353
Size: 475 5049
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4531 809
Size: 507 3215 1802
Fragments arranged by size:
3215 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2043 2123 2408
Size: 54 1425 50 275 18 213 80 285
Cuts at: 2408 2445 2646 2971 3860 5489 8
Size: 37 201 325 889 1629 43
Fragments arranged by size:
1629 1425 889 325 285 275 213 201
80 54 50 43 37 18
EagI C'GGCC_G
Cuts at: 1537 1812 2043 2408 2971 1537
Size: 275 231 365 563 4090
Fragments arranged by size:
4090 563 365 275 231
EarI CTCTTCn'nnn_
Cuts at: 1353 2352 3454 1353
Size: 999 1102 3423
Fragments arranged by size:
3423 1102 999
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4111 4485 4631 387
Size: 1064 138 2522 374 146 1280
Fragments arranged by size:
2522 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2041 2539 2660 3587 4184 4971 5239
Size: 393 409 498 121 927 597 787 268
Cuts at: 5239 1239
Size: 1524
Fragments arranged by size:
1524 927 787 597 498 409 393 268
121
EcoO109I rG'GnC_Cy
Cuts at: 2089 2090 2233 2234 2089
Size: 1 143 1 5379
Fragments arranged by size:
5379 143 1 1
EcoRI G'AATT_C
Cuts at: 2833 3211 2833
Size: 378 5146
FspI TGC'GCA
Cuts at: 4030 4030
Size: 5524
HaeII r_GCGC'y
Cuts at: 1678 2192 2427 2521 4671 1678
Size: 514 235 94 2150 2531
Fragments arranged by size:
2531 2150 514 235 94
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4165 4197 4239 4271 1373
Size: 32 135 32 2593 32 42 32 2626
Fragments arranged by size:
2626 2593 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2868 3114 669
Size: 988 281 930 246 3079
Fragments arranged by size:
3079 988 930 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5524
HpaI GTT'AAC
Cuts at: 3114 3114
Size: 5524
KasI G'GCGC_C
Cuts at: 2188 2188
Size: 5524
KpnI G_GTAC'C
Cuts at: 2277 2849 2277
Size: 572 4952
MfeI C'AATT_G
Cuts at: 3123 3123
Size: 5524
MluI A'CGCG_T
Cuts at: 2661 5400 2661
Size: 2739 2785
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4638 4822 1305
Size: 3333 184 2007
Fragments arranged by size:
3333 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2125 10
Size: 54 1425 343 293 3409
Fragments arranged by size:
3409 1425 343 293 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3482 3841 4000 518
Size: 648 2316 359 159 2042
Fragments arranged by size:
2316 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2107 2289 2381 2413 2692 2940 3617 4765
Size: 501 182 92 32 279 248 677 1148
Cuts at: 4765 5010 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 501 279 248 245 182
92 32
NaeI GCC'GGC
Cuts at: 2440 2440
Size: 5524
NarI GG'CG_CC
Cuts at: 2189 2189
Size: 5524
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4033
Fragments arranged by size:
4033 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5524
NgoMIV G'CCGG_C
Cuts at: 2438 2438
Size: 5524
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5524
NotI GC'GGCC_GC
Cuts at: 2408 2971 2408
Size: 563 4961
NspI r_CATG'y
Cuts at: 1132 1229 2689 2882 4427 1132
Size: 97 1460 193 1545 2229
Fragments arranged by size:
2229 1545 1460 193 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5524
PciI A'CATG_T
Cuts at: 4423 4423
Size: 5524
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5524
PfoI T'CCnGG_A
Cuts at: 2700 2700
Size: 5524
PmeI GTTT'AAAC
Cuts at: 2828 2828
Size: 5524
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3593 3625 5106 5138 3593
Size: 32 1481 32 3979
Fragments arranged by size:
3979 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5524
PsiI TTA'TAA
Cuts at: 3094 3094
Size: 5524
PspOMI G'GGCC_C
Cuts at: 2089 2233 2299 2089
Size: 144 66 5314
Fragments arranged by size:
5314 144 66
PspXI vC'TCGA_Gb
Cuts at: 2495 2495
Size: 5524
PstI C_TGCA'G
Cuts at: 830 2565 2876 830
Size: 1735 311 3478
Fragments arranged by size:
3478 1735 311
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5524
SacII CC_GC'GG
Cuts at: 2108 2382 2414 2108
Size: 274 32 5218
Fragments arranged by size:
5218 274 32
SalI G'TCGA_C
Cuts at: 1936 2866 1936
Size: 930 4594
SbfI CC_TGCA'GG
Cuts at: 2876 2876
Size: 5524
ScaI AGT'ACT
Cuts at: 3772 5484 3772
Size: 1712 3812
SfcI C'TryA_G
Cuts at: 826 1045 2561 2872 4007 4688 4879 826
Size: 219 1516 311 1135 681 191 1471
Fragments arranged by size:
1516 1471 1135 681 311 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2444 2444
Size: 5524
SfoI GGC'GCC
Cuts at: 2190 2190
Size: 5524
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5524
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5524
SmaI CCC'GGG
Cuts at: 1872 2304 2769 2851 1872
Size: 432 465 82 4545
Fragments arranged by size:
4545 465 432 82
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2495 3628 4529 4791 820
Size: 197 926 552 1133 901 262 1553
Fragments arranged by size:
1553 1133 926 901 552 262 197
SnaBI TAC'GTA
Cuts at: 493 3315 493
Size: 2822 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5524
SphI G_CATG'C
Cuts at: 1132 2689 2882 1132
Size: 1557 193 3774
Fragments arranged by size:
3774 1557 193
SrfI GCCC'GGGC
Cuts at: 2304 2304
Size: 5524
SspI AAT'ATT
Cuts at: 5 52 3448 5
Size: 47 3396 2081
Fragments arranged by size:
3396 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5524
StyI C'CwwG_G
Cuts at: 513 1065 2004 2280 2963 513
Size: 552 939 276 683 3074
Fragments arranged by size:
3074 939 683 552 276
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3551 3568 3721 3906 4351 325
Size: 1255 1971 17 153 185 445 1498
Fragments arranged by size:
1971 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3770 5482 96
Size: 274 80 33 51 1443 1793 1712 138
Fragments arranged by size:
1793 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5484 5516 5484
Size: 32 5492
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5524
XbaI T'CTAG_A
Cuts at: 2860 2860
Size: 5524
XhoI C'TCGA_G
Cuts at: 1943 2495 1943
Size: 552 4972
XmaI C'CCGG_G
Cuts at: 1870 2302 2767 2849 1870
Size: 432 465 82 4545
Fragments arranged by size:
4545 465 432 82
XmnI GAAnn'nnTTC
Cuts at: 3653 3653
Size: 5524
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3332 276
Size: 53 83 186 936 1798 2468
Fragments arranged by size:
2468 1798 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PstI
PvuI SacII SalI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI NruI NsiI PacI PmlI PpuMI PsrI
PvuII RsrII SacI SanDI SapI SexAI SwaI XcmI