Restriction map for
pFN21AB8926
(Circular) (Six-base) MAPSORT of: pFN21AB8926.seq Check: 859 from: 1 to: 5524
pFN21AB8926 5524 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3334 278
Size: 53 83 186 936 1798 2468
Fragments arranged by size:
2468 1798 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2867 1937
Size: 930 4594
Acc65I G'GTAC_C
Cuts at: 2845 2845
Size: 5524
AclI AA'CG_TT
Cuts at: 1563 2282 3651 4024 1563
Size: 719 1369 373 3063
Fragments arranged by size:
3063 1369 719 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2106 2329 2413 2497 2581 2666 3587 4971
Size: 474 223 84 84 84 85 921 1384
Cuts at: 4971 5239 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 921 474 268 223 85 84
84 84
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5327
AflIII A'CryG_T
Cuts at: 1203 4423 5400 1203
Size: 3220 977 1327
Fragments arranged by size:
3220 1327 977
AgeI A'CCGG_T
Cuts at: 4355 4355
Size: 5524
AhdI GACnn_n'nnGTC
Cuts at: 2228 4253 2228
Size: 2025 3499
AleI CACnn'nnGTG
Cuts at: 1166 2556 1166
Size: 1390 4134
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5524
AlwNI CAG_nnn'CTG
Cuts at: 3267 4839 3267
Size: 1572 3952
ApaLI G'TGCA_C
Cuts at: 2323 2789 3581 4737 2323
Size: 466 792 1156 3110
Fragments arranged by size:
3110 1156 792 466
ApoI r'AATT_y
Cuts at: 1493 2146 2833 3059 3211 5320 1493
Size: 653 687 226 152 2109 1697
Fragments arranged by size:
2109 1697 687 653 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4078 160
Size: 872 3046 1606
Fragments arranged by size:
3046 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5524
AvaI C'yCGr_G
Cuts at: 1870 1943 2849 1870
Size: 73 906 4545
Fragments arranged by size:
4545 906 73
BamHI G'GATC_C
Cuts at: 2123 2854 3221 2123
Size: 731 367 4426
Fragments arranged by size:
4426 731 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2845 4300 618
Size: 325 217 627 1058 1455 1842
Fragments arranged by size:
1842 1455 1058 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2843 1391
Size: 1452 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2198 928
Size: 971 299 4254
Fragments arranged by size:
4254 971 299
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3715 3749 1903
Size: 34 1778 34 3678
Fragments arranged by size:
3678 1778 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2373 3416 4626 2373
Size: 1043 1210 3271
Fragments arranged by size:
3271 1210 1043
BclI T'GATC_A
Cuts at: 1548 2578 1548
Size: 1030 4494
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4135 136
Size: 107 122 71 631 605 2463 1525
Fragments arranged by size:
2463 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5519 5519
Size: 5524
BlpI GC'TnA_GC
Cuts at: 2941 2941
Size: 5524
Bme1580I G_kGCm'C
Cuts at: 1790 2327 2793 3585 4741 1790
Size: 537 466 792 1156 2573
Fragments arranged by size:
2573 1156 792 537 466
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5524
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2659 3255 4213
Size: 416 53 83 399 24 1242 596 958
Cuts at: 4213 442
Size: 1753
Fragments arranged by size:
1753 1242 958 596 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5524
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2462 2462
Size: 5524
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2235 4184 1239
Size: 996 1949 2579
Fragments arranged by size:
2579 1949 996
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2113 3649 4514 4812 2113
Size: 1536 865 298 2825
Fragments arranged by size:
2825 1536 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4187 882
Size: 617 2688 2219
Fragments arranged by size:
2688 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3315 493
Size: 711 2111 2702
Fragments arranged by size:
2702 2111 711
BsaBI GATnn'nnATC
Cuts at: 5518 5518
Size: 5524
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3331 3713 4348 275
Size: 53 83 186 936 1798 382 635 1451
Fragments arranged by size:
1798 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3957 4355 4629 4776 5187 3957
Size: 398 274 147 411 4294
Fragments arranged by size:
4294 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5299
Fragments arranged by size:
5299 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5524
BseYI C'CCAG_C
Cuts at: 2133 4727 5223 2133
Size: 2594 496 2434
Fragments arranged by size:
2594 2434 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2689 1163
Size: 573 953 3998
Fragments arranged by size:
3998 953 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2974 3735 4763 1540
Size: 275 186 973 761 1028 2301
Fragments arranged by size:
2301 1028 973 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2327 2793 3585 3670 4741 2327
Size: 466 792 85 1071 3110
Fragments arranged by size:
3110 1071 792 466 85
BsmI GAATG_Cn'
Cuts at: 3035 3128 3035
Size: 93 5431
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2327 2793 2843 3585 3670 4741 1391
Size: 399 537 466 50 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 537 466 399 85 50
BspEI T'CCGG_A
Cuts at: 5187 5187
Size: 5524
BspHI T'CATG_A
Cuts at: 3413 5143 3413
Size: 1730 3794
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2879 844
Size: 2035 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3411 1394
Size: 429 1588 3507
Fragments arranged by size:
3507 1588 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4019 4193 65
Size: 1183 2771 174 1396
Fragments arranged by size:
2771 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4168 4355 1700
Size: 110 2358 187 2869
Fragments arranged by size:
2869 2358 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5524
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5524
BssSI C'ACGA_G
Cuts at: 2456 3584 4596 2456
Size: 1128 1012 3384
Fragments arranged by size:
3384 1128 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5524
BstBI TT'CG_AA
Cuts at: 2189 4328 2189
Size: 2139 3385
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5524
BstXI CCAn_nnnn'nTGG
Cuts at: 2249 2249
Size: 5524
BstYI r'GATC_y
Cuts at: 1972 2123 2854 3221 3606 3623 5075 5161 5173
Size: 151 731 367 385 17 1452 86 12
Cuts at: 5173 5519 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 731 385 367 346 151 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4972
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4785
Fragments arranged by size:
4785 683 56
BtsI GCAGTG_nn'
Cuts at: 3040 3834 3854 3040
Size: 794 20 4710
Fragments arranged by size:
4710 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5524
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2398 2433 529
Size: 35 1834 35 3620
Fragments arranged by size:
3620 1834 35 35
DraI TTT'AAA
Cuts at: 2828 2828
Size: 5524
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4531 809
Size: 507 3215 1802
Fragments arranged by size:
3215 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2746 2971 3860
Size: 54 1425 50 275 18 916 225 889
Cuts at: 3860 5489 8
Size: 1629 43
Fragments arranged by size:
1629 1425 916 889 275 225 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2971 1537
Size: 275 1159 4090
Fragments arranged by size:
4090 1159 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2152 2669 3454 1353
Size: 799 517 785 3423
Fragments arranged by size:
3423 799 785 517
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4111 4485 4631 387
Size: 1064 138 2522 374 146 1280
Fragments arranged by size:
2522 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2106 2235 2329 2413 2497 2581 2666
Size: 393 474 129 94 84 84 84 85
Cuts at: 2666 3587 4184 4971 5239 1239
Size: 921 597 787 268 1524
Fragments arranged by size:
1524 921 787 597 474 393 268 129
94 85 84 84 84
EcoRI G'AATT_C
Cuts at: 2833 3211 2833
Size: 378 5146
EcoRV GAT'ATC
Cuts at: 2140 2140
Size: 5524
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2378 2378
Size: 5524
FspI TGC'GCA
Cuts at: 4030 4030
Size: 5524
HaeII r_GCGC'y
Cuts at: 1678 4671 1678
Size: 2993 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2214 2246 2722 2754 2764
Size: 32 135 32 642 32 476 32 10
Cuts at: 2764 2796 4165 4197 4239 4271 1373
Size: 32 1369 32 42 32 2626
Fragments arranged by size:
2626 1369 642 476 135 42 32 32
32 32 32 32 32 10
HincII GTy'rAC
Cuts at: 669 1657 1938 2435 2868 3114 669
Size: 988 281 497 433 246 3079
Fragments arranged by size:
3079 988 497 433 281 246
HindIII A'AGCT_T
Cuts at: 748 2341 2425 2509 2611 2761 748
Size: 1593 84 84 102 150 3511
Fragments arranged by size:
3511 1593 150 102 84 84
HpaI GTT'AAC
Cuts at: 3114 3114
Size: 5524
KpnI G_GTAC'C
Cuts at: 2849 2849
Size: 5524
MfeI C'AATT_G
Cuts at: 3123 3123
Size: 5524
MluI A'CGCG_T
Cuts at: 5400 5400
Size: 5524
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2042 4638 4822 1305
Size: 737 2596 184 2007
Fragments arranged by size:
2596 2007 737 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3702
Fragments arranged by size:
3702 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2100 2556 3482 3841 4000 518
Size: 648 934 456 926 359 159 2042
Fragments arranged by size:
2042 934 926 648 456 359 159
MspA1I CmG'CkG
Cuts at: 1606 2940 3617 4765 5010 1606
Size: 1334 677 1148 245 2120
Fragments arranged by size:
2120 1334 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4972
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5524
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5524
NotI GC'GGCC_GC
Cuts at: 2971 2971
Size: 5524
NspI r_CATG'y
Cuts at: 1132 1229 2882 4427 1132
Size: 97 1653 1545 2229
Fragments arranged by size:
2229 1653 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5524
PciI A'CATG_T
Cuts at: 4423 4423
Size: 5524
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5524
PmeI GTTT'AAAC
Cuts at: 2828 2828
Size: 5524
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3593 3625 5106 5138 3593
Size: 32 1481 32 3979
Fragments arranged by size:
3979 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5524
PsiI TTA'TAA
Cuts at: 3094 3094
Size: 5524
PstI C_TGCA'G
Cuts at: 830 2876 830
Size: 2046 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5524
SalI G'TCGA_C
Cuts at: 1936 2866 1936
Size: 930 4594
SbfI CC_TGCA'GG
Cuts at: 2876 2876
Size: 5524
ScaI AGT'ACT
Cuts at: 3772 5484 3772
Size: 1712 3812
SfcI C'TryA_G
Cuts at: 826 1045 2118 2198 2569 2872 4007 4688 4879
Size: 219 1073 80 371 303 1135 681 191
Cuts at: 4879 826
Size: 1471
Fragments arranged by size:
1471 1135 1073 681 371 303 219 191
80
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5524
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5524
SmaI CCC'GGG
Cuts at: 1872 2851 1872
Size: 979 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2128 3628 4529 4791 820
Size: 197 926 185 1500 901 262 1553
Fragments arranged by size:
1553 1500 926 901 262 197 185
SnaBI TAC'GTA
Cuts at: 493 3315 493
Size: 2822 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5524
SphI G_CATG'C
Cuts at: 1132 2882 1132
Size: 1750 3774
SspI AAT'ATT
Cuts at: 5 52 3448 5
Size: 47 3396 2081
Fragments arranged by size:
3396 2081 47
StuI AGG'CCT
Cuts at: 1517 2643 1517
Size: 1126 4398
StyI C'CwwG_G
Cuts at: 513 1065 2963 513
Size: 552 1898 3074
Fragments arranged by size:
3074 1898 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2342 2783 3551 3568 3721 3906 4351
Size: 1255 762 441 768 17 153 185 445
Cuts at: 4351 325
Size: 1498
Fragments arranged by size:
1498 1255 768 762 445 441 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3770 5482 96
Size: 274 80 33 51 1443 1793 1712 138
Fragments arranged by size:
1793 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5484 5516 5484
Size: 32 5492
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5524
XbaI T'CTAG_A
Cuts at: 2860 2860
Size: 5524
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5524
XmaI C'CCGG_G
Cuts at: 1870 2849 1870
Size: 979 4545
XmnI GAAnn'nnTTC
Cuts at: 2426 2678 3653 2426
Size: 252 975 4297
Fragments arranged by size:
4297 975 252
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3332 276
Size: 53 83 186 936 1798 2468
Fragments arranged by size:
2468 1798 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI