Restriction map for
pFN21AB8925
(Circular) (Six-base) MAPSORT of: pFN21AB8925.seq Check: 9610 from: 1 to: 5524
pFN21AB8925 5524 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3334 278
Size: 53 83 186 936 1798 2468
Fragments arranged by size:
2468 1798 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2867 1937
Size: 930 4594
Acc65I G'GTAC_C
Cuts at: 2457 2845 2457
Size: 388 5136
AclI AA'CG_TT
Cuts at: 1563 3651 4024 1563
Size: 2088 373 3063
Fragments arranged by size:
3063 2088 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2207 2237 3587 4971 5239 1632
Size: 575 30 1350 1384 268 1917
Fragments arranged by size:
1917 1384 1350 575 268 30
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5327
AflIII A'CryG_T
Cuts at: 1203 4423 5400 1203
Size: 3220 977 1327
Fragments arranged by size:
3220 1327 977
AgeI A'CCGG_T
Cuts at: 4355 4355
Size: 5524
AhdI GACnn_n'nnGTC
Cuts at: 4253 4253
Size: 5524
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5524
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5524
AlwNI CAG_nnn'CTG
Cuts at: 2179 2671 2728 3267 4839 2179
Size: 492 57 539 1572 2864
Fragments arranged by size:
2864 1572 539 492 57
ApaI G_GGCC'C
Cuts at: 2121 2121
Size: 5524
ApaLI G'TGCA_C
Cuts at: 3581 4737 3581
Size: 1156 4368
ApoI r'AATT_y
Cuts at: 1493 2833 3059 3211 5320 1493
Size: 1340 226 152 2109 1697
Fragments arranged by size:
2109 1697 1340 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4078 160
Size: 872 3046 1606
Fragments arranged by size:
3046 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5524
AvaI C'yCGr_G
Cuts at: 1870 1943 2120 2359 2471 2562 2849 1870
Size: 73 177 239 112 91 287 4545
Fragments arranged by size:
4545 287 239 177 112 91 73
BamHI G'GATC_C
Cuts at: 2854 3221 2854
Size: 367 5157
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2156 2284 2457 2467 2845
Size: 325 217 627 369 128 173 10 378
Cuts at: 2845 4300 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 378 369 325 217 173
128 10
BanII G_rGCy'C
Cuts at: 1391 2084 2121 2322 2843 1391
Size: 693 37 201 521 4072
Fragments arranged by size:
4072 693 521 201 37
BbeI G_GCGC'C
Cuts at: 2160 2288 2471 2160
Size: 128 183 5213
Fragments arranged by size:
5213 183 128
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4553
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3715 3749 1903
Size: 34 1778 34 3678
Fragments arranged by size:
3678 1778 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3416 4626 3416
Size: 1210 4314
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5524
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2135 2149 4135
Size: 107 122 71 631 605 463 14 1986
Cuts at: 4135 136
Size: 1525
Fragments arranged by size:
1986 1525 631 605 463 122 107 71
14
BglII A'GATC_T
Cuts at: 2523 5519 2523
Size: 2996 2528
BlpI GC'TnA_GC
Cuts at: 2941 2941
Size: 5524
Bme1580I G_kGCm'C
Cuts at: 1790 2121 2622 3585 4741 1790
Size: 331 501 963 1156 2573
Fragments arranged by size:
2573 1156 963 501 331
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5524
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2746 2770 3255
Size: 416 53 83 399 24 1329 24 485
Cuts at: 3255 4213 442
Size: 958 1753
Fragments arranged by size:
1753 1329 958 485 416 399 83 53
24 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5524
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2098 2098
Size: 5524
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2453 4184 1239
Size: 1214 1731 2579
Fragments arranged by size:
2579 1731 1214
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2284 3649 4514 4812 2284
Size: 1365 865 298 2996
Fragments arranged by size:
2996 1365 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2348 4187 882
Size: 617 849 1839 2219
Fragments arranged by size:
2219 1839 849 617
BsaAI yAC'GTr
Cuts at: 493 1204 3315 493
Size: 711 2111 2702
Fragments arranged by size:
2702 2111 711
BsaBI GATnn'nnATC
Cuts at: 5518 5518
Size: 5524
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2157 2285 2376 2468
Size: 53 83 186 936 624 128 91 92
Cuts at: 2468 2588 3331 3713 4348 275
Size: 120 743 382 635 1451
Fragments arranged by size:
1451 936 743 635 624 382 186 128
120 92 91 83 53
BsaWI w'CCGG_w
Cuts at: 2098 2338 3957 4355 4629 4776 5187 2098
Size: 240 1619 398 274 147 411 2435
Fragments arranged by size:
2435 1619 411 398 274 240 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2288 2318 1178
Size: 30 165 30 885 30 4384
Fragments arranged by size:
4384 885 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2090 1186
Size: 904 4620
BseYI C'CCAG_C
Cuts at: 2146 2808 4727 5223 2146
Size: 662 1919 496 2447
Fragments arranged by size:
2447 1919 662 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2192 2249 1163
Size: 573 456 57 4438
Fragments arranged by size:
4438 573 456 57
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2328 2974 3735 4763 1540
Size: 275 186 327 646 761 1028 2301
Fragments arranged by size:
2301 1028 761 646 327 275 186
BsiHKAI G_wGCw'C
Cuts at: 3585 3670 4741 3585
Size: 85 1071 4368
Fragments arranged by size:
4368 1071 85
BsmI GAATG_Cn'
Cuts at: 2697 3035 3128 2697
Size: 338 93 5093
Fragments arranged by size:
5093 338 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2084 2121 2322 2622 2843 3585 3670
Size: 399 294 37 201 300 221 742 85
Cuts at: 3670 4741 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 300 294 221 201
85 37
BspEI T'CCGG_A
Cuts at: 2098 5187 2098
Size: 3089 2435
BspHI T'CATG_A
Cuts at: 3413 5143 3413
Size: 1730 3794
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2064 2506 2879 844
Size: 1220 442 373 3489
Fragments arranged by size:
3489 1220 442 373
BsrBI CCG'CTC
Cuts at: 1394 1823 2437 3411 1394
Size: 429 614 974 3507
Fragments arranged by size:
3507 974 614 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4019 4193 65
Size: 1183 2771 174 1396
Fragments arranged by size:
2771 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2378 4168 4355 1700
Size: 110 568 1790 187 2869
Fragments arranged by size:
2869 1790 568 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5524
BssHII G'CGCG_C
Cuts at: 1925 2557 2718 1925
Size: 632 161 4731
Fragments arranged by size:
4731 632 161
BssSI C'ACGA_G
Cuts at: 3584 4596 3584
Size: 1012 4512
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2179 2728 2737 794
Size: 1385 549 9 3581
Fragments arranged by size:
3581 1385 549 9
BstBI TT'CG_AA
Cuts at: 4328 4328
Size: 5524
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5524
BstYI r'GATC_y
Cuts at: 1972 2523 2854 3221 3606 3623 5075 5161 5173
Size: 551 331 367 385 17 1452 86 12
Cuts at: 5173 5519 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 551 385 367 346 331 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2292 2292
Size: 5524
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4972
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4785
Fragments arranged by size:
4785 683 56
BtsI GCAGTG_nn'
Cuts at: 2819 3040 3834 3854 2819
Size: 221 794 20 4489
Fragments arranged by size:
4489 794 221 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5524
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5489
DraI TTT'AAA
Cuts at: 2828 2828
Size: 5524
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4531 809
Size: 507 3215 1802
Fragments arranged by size:
3215 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2088 2235 2325
Size: 54 1425 50 275 18 258 147 90
Cuts at: 2325 2487 2613 2763 2971 3860 5489 8
Size: 162 126 150 208 889 1629 43
Fragments arranged by size:
1629 1425 889 275 258 208 162 150
147 126 90 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2325 2971 1537
Size: 275 513 646 4090
Fragments arranged by size:
4090 646 513 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2100 3454 1353
Size: 747 1354 3423
Fragments arranged by size:
3423 1354 747
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2489 4111 4485 4631 387
Size: 1064 138 900 1622 374 146 1280
Fragments arranged by size:
1622 1280 1064 900 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2207 2237 2453 3587 4184 4971 5239
Size: 393 575 30 216 1134 597 787 268
Cuts at: 5239 1239
Size: 1524
Fragments arranged by size:
1524 1134 787 597 575 393 268 216
30
EcoNI CCTnn'n_nnAGG
Cuts at: 2801 2801
Size: 5524
EcoO109I rG'GnC_Cy
Cuts at: 2117 2248 2668 2117
Size: 131 420 4973
Fragments arranged by size:
4973 420 131
EcoRI G'AATT_C
Cuts at: 2833 3211 2833
Size: 378 5146
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2065 2212 2065
Size: 147 5377
FspI TGC'GCA
Cuts at: 4030 4030
Size: 5524
HaeII r_GCGC'y
Cuts at: 1678 2160 2288 2443 2471 4671 1678
Size: 482 128 155 28 2200 2531
Fragments arranged by size:
2531 2200 482 155 128 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2288 2320 4165 4197 4239
Size: 32 135 32 716 32 1845 32 42
Cuts at: 4239 4271 1373
Size: 32 2626
Fragments arranged by size:
2626 1845 716 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2868 3114 669
Size: 988 281 930 246 3079
Fragments arranged by size:
3079 988 930 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5524
HpaI GTT'AAC
Cuts at: 3114 3114
Size: 5524
KasI G'GCGC_C
Cuts at: 2156 2284 2467 2156
Size: 128 183 5213
Fragments arranged by size:
5213 183 128
KpnI G_GTAC'C
Cuts at: 2461 2849 2461
Size: 388 5136
MfeI C'AATT_G
Cuts at: 3123 3123
Size: 5524
MluI A'CGCG_T
Cuts at: 5400 5400
Size: 5524
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4638 4822 1305
Size: 3333 184 2007
Fragments arranged by size:
3333 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2237 2489 10
Size: 54 1425 343 405 252 3045
Fragments arranged by size:
3045 1425 405 343 252 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3482 3841 4000 518
Size: 648 2316 359 159 2042
Fragments arranged by size:
2316 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2146 2455 2940 3617 4765 5010 1606
Size: 540 309 485 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 540 485 309 245
NaeI GCC'GGC
Cuts at: 2380 2380
Size: 5524
NarI GG'CG_CC
Cuts at: 2157 2285 2468 2157
Size: 128 183 5213
Fragments arranged by size:
5213 183 128
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4972
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5524
NgoMIV G'CCGG_C
Cuts at: 2378 2378
Size: 5524
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5524
NotI GC'GGCC_GC
Cuts at: 2971 2971
Size: 5524
NspI r_CATG'y
Cuts at: 1132 1229 2740 2882 4427 1132
Size: 97 1511 142 1545 2229
Fragments arranged by size:
2229 1545 1511 142 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5524
PciI A'CATG_T
Cuts at: 4423 4423
Size: 5524
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2671 1108
Size: 1563 3961
PmeI GTTT'AAAC
Cuts at: 2828 2828
Size: 5524
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3593 3625 5106 5138 3593
Size: 32 1481 32 3979
Fragments arranged by size:
3979 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2668 2668
Size: 5524
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5524
PsiI TTA'TAA
Cuts at: 3094 3094
Size: 5524
PspOMI G'GGCC_C
Cuts at: 2117 2117
Size: 5524
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2183 2215 2528 2560 2183
Size: 32 313 32 5147
Fragments arranged by size:
5147 313 32 32
PstI C_TGCA'G
Cuts at: 830 2733 2876 830
Size: 1903 143 3478
Fragments arranged by size:
3478 1903 143
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5524
SalI G'TCGA_C
Cuts at: 1936 2866 1936
Size: 930 4594
SbfI CC_TGCA'GG
Cuts at: 2876 2876
Size: 5524
ScaI AGT'ACT
Cuts at: 2258 3772 5484 2258
Size: 1514 1712 2298
Fragments arranged by size:
2298 1712 1514
SfcI C'TryA_G
Cuts at: 826 1045 2713 2729 2872 4007 4688 4879 826
Size: 219 1668 16 143 1135 681 191 1471
Fragments arranged by size:
1668 1471 1135 681 219 191 143 16
SfoI GGC'GCC
Cuts at: 2158 2286 2469 2158
Size: 128 183 5213
Fragments arranged by size:
5213 183 128
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5524
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5524
SmaI CCC'GGG
Cuts at: 1872 2361 2564 2851 1872
Size: 489 203 287 4545
Fragments arranged by size:
4545 489 287 203
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2299 3628 4529 4791 820
Size: 197 926 356 1329 901 262 1553
Fragments arranged by size:
1553 1329 926 901 356 262 197
SnaBI TAC'GTA
Cuts at: 493 3315 493
Size: 2822 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5524
SphI G_CATG'C
Cuts at: 1132 2882 1132
Size: 1750 3774
SrfI GCCC'GGGC
Cuts at: 2564 2564
Size: 5524
SspI AAT'ATT
Cuts at: 5 52 3448 5
Size: 47 3396 2081
Fragments arranged by size:
3396 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5524
StyI C'CwwG_G
Cuts at: 513 1065 2412 2963 513
Size: 552 1347 551 3074
Fragments arranged by size:
3074 1347 552 551
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2026 3551 3568 3721 3906 4351 325
Size: 1255 446 1525 17 153 185 445 1498
Fragments arranged by size:
1525 1498 1255 446 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2256 2742 2754
Size: 274 80 33 51 1443 279 486 12
Cuts at: 2754 3770 5482 96
Size: 1016 1712 138
Fragments arranged by size:
1712 1443 1016 486 279 274 138 80
51 33 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5484 5516 5484
Size: 32 5492
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5524
XbaI T'CTAG_A
Cuts at: 2860 2860
Size: 5524
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5524
XmaI C'CCGG_G
Cuts at: 1870 2359 2562 2849 1870
Size: 489 203 287 4545
Fragments arranged by size:
4545 489 287 203
XmnI GAAnn'nnTTC
Cuts at: 2195 3653 2195
Size: 1458 4066
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3332 276
Size: 53 83 186 936 1798 2468
Fragments arranged by size:
2468 1798 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV
FseI FspAI NruI NsiI PacI PfoI PmlI PspXI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SwaI XcmI