Restriction map for
pFN21AB8923
(Circular) (Six-base) MAPSORT of: pFN21AB8923.seq Check: 6931 from: 1 to: 5515
pFN21AB8923 5515 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3325 278
Size: 53 83 186 936 1789 2468
Fragments arranged by size:
2468 1789 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2858 1937
Size: 921 4594
Acc65I G'GTAC_C
Cuts at: 2836 2836
Size: 5515
AclI AA'CG_TT
Cuts at: 1563 3642 4015 1563
Size: 2079 373 3063
Fragments arranged by size:
3063 2079 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3578 4962 5230 1632
Size: 1946 1384 268 1917
Fragments arranged by size:
1946 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5318
AflIII A'CryG_T
Cuts at: 1203 4414 5391 1203
Size: 3211 977 1327
Fragments arranged by size:
3211 1327 977
AgeI A'CCGG_T
Cuts at: 4346 4346
Size: 5515
AhdI GACnn_n'nnGTC
Cuts at: 4244 4244
Size: 5515
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5515
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2502 1184
Size: 1318 4197
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2627 2659 2627
Size: 32 5483
AlwNI CAG_nnn'CTG
Cuts at: 2626 3258 4830 2626
Size: 632 1572 3311
Fragments arranged by size:
3311 1572 632
ApaI G_GGCC'C
Cuts at: 2599 2599
Size: 5515
ApaLI G'TGCA_C
Cuts at: 3572 4728 3572
Size: 1156 4359
ApoI r'AATT_y
Cuts at: 1493 2824 3050 3202 5311 1493
Size: 1331 226 152 2109 1697
Fragments arranged by size:
2109 1697 1331 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4069 160
Size: 872 3037 1606
Fragments arranged by size:
3037 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5515
AvaI C'yCGr_G
Cuts at: 1870 1943 2179 2840 1870
Size: 73 236 661 4545
Fragments arranged by size:
4545 661 236 73
BamHI G'GATC_C
Cuts at: 2845 3212 2845
Size: 367 5148
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2242 2384 2509 2836 4291
Size: 325 217 627 455 142 125 327 1455
Cuts at: 4291 618
Size: 1842
Fragments arranged by size:
1842 1455 627 455 327 325 217 142
125
BanII G_rGCy'C
Cuts at: 1391 2012 2186 2256 2507 2599 2834 1391
Size: 621 174 70 251 92 235 4072
Fragments arranged by size:
4072 621 251 235 174 92 70
BbeI G_GCGC'C
Cuts at: 2246 2388 2513 2246
Size: 142 125 5248
Fragments arranged by size:
5248 142 125
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4544
BbvCI CC'TCA_GC
Cuts at: 2292 2292
Size: 5515
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3706 3740 1903
Size: 34 1769 34 3678
Fragments arranged by size:
3678 1769 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3407 4617 3407
Size: 1210 4305
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5515
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2181 2196 2240
Size: 107 122 71 631 605 509 15 44
Cuts at: 2240 2352 2586 2603 4126 136
Size: 112 234 17 1523 1525
Fragments arranged by size:
1525 1523 631 605 509 234 122 112
107 71 44 17 15
BglII A'GATC_T
Cuts at: 2529 5510 2529
Size: 2981 2534
BlpI GC'TnA_GC
Cuts at: 2932 2932
Size: 5515
Bme1580I G_kGCm'C
Cuts at: 1790 2599 3576 4732 1790
Size: 809 977 1156 2573
Fragments arranged by size:
2573 1156 977 809
BmgBI CAC'GTC
Cuts at: 1318 2277 1318
Size: 959 4556
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3246 4204 442
Size: 416 53 83 399 24 1829 958 1753
Fragments arranged by size:
1829 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5515
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2362 2648 2654 4175 1239
Size: 1123 286 6 1521 2579
Fragments arranged by size:
2579 1521 1123 286 6
Bpu10I CC'TnA_GC
Cuts at: 2292 2369 2292
Size: 77 5438
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3640 4505 4803 3640
Size: 865 298 4352
Fragments arranged by size:
4352 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4178 882
Size: 617 2679 2219
Fragments arranged by size:
2679 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3306 493
Size: 711 2102 2702
Fragments arranged by size:
2702 2102 711
BsaBI GATnn'nnATC
Cuts at: 5509 5509
Size: 5515
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2243 2385 2397 2441
Size: 53 83 186 936 710 142 12 44
Cuts at: 2441 2460 2510 3322 3704 4339 275
Size: 19 50 812 382 635 1451
Fragments arranged by size:
1451 936 812 710 635 382 186 142
83 53 50 44 19 12
BsaWI w'CCGG_w
Cuts at: 2201 2444 2739 3948 4346 4620 4767 5178 2201
Size: 243 295 1209 398 274 147 411 2538
Fragments arranged by size:
2538 1209 411 398 295 274 243 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2183 2213 2272 2302 2627
Size: 30 165 30 780 30 59 30 325
Cuts at: 2627 2657 1178
Size: 30 4036
Fragments arranged by size:
4036 780 325 165 59 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2280 2468 1186
Size: 1094 188 4233
Fragments arranged by size:
4233 1094 188
BseYI C'CCAG_C
Cuts at: 4718 5214 4718
Size: 496 5019
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2537 1163
Size: 573 801 4141
Fragments arranged by size:
4141 801 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2038 2176 2394 2965 3726 4754
Size: 275 186 37 138 218 571 761 1028
Cuts at: 4754 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 571 275 218 186 138
37
BsiHKAI G_wGCw'C
Cuts at: 2012 3576 3661 4732 2012
Size: 1564 85 1071 2795
Fragments arranged by size:
2795 1564 1071 85
BsmI GAATG_Cn'
Cuts at: 3026 3119 3026
Size: 93 5422
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2186 2256 2507 2599 2834 3576
Size: 399 222 174 70 251 92 235 742
Cuts at: 3576 3661 4732 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 251 235 222 174
92 85 70
BspEI T'CCGG_A
Cuts at: 2201 2444 5178 2201
Size: 243 2734 2538
Fragments arranged by size:
2734 2538 243
BspHI T'CATG_A
Cuts at: 2563 3404 5134 2563
Size: 841 1730 2944
Fragments arranged by size:
2944 1730 841
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2124 2870 844
Size: 1280 746 3489
Fragments arranged by size:
3489 1280 746
BsrBI CCG'CTC
Cuts at: 1394 1823 2178 3402 1394
Size: 429 355 1224 3507
Fragments arranged by size:
3507 1224 429 355
BsrDI GCAATG_nn'
Cuts at: 65 1248 4010 4184 65
Size: 1183 2762 174 1396
Fragments arranged by size:
2762 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2269 2399 4159 4346 1700
Size: 110 459 130 1760 187 2869
Fragments arranged by size:
2869 1760 459 187 130 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5515
BssHII G'CGCG_C
Cuts at: 1925 2209 1925
Size: 284 5231
BssSI C'ACGA_G
Cuts at: 3575 4587 3575
Size: 1012 4503
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5515
BstBI TT'CG_AA
Cuts at: 4319 4319
Size: 5515
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5515
BstYI r'GATC_y
Cuts at: 1972 2161 2529 2845 3212 3597 3614 5066 5152
Size: 189 368 316 367 385 17 1452 86
Cuts at: 5152 5164 5510 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 368 367 346 316 189
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2319 2350 2790 513
Size: 552 1254 31 440 3238
Fragments arranged by size:
3238 1254 552 440 31
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4776
Fragments arranged by size:
4776 683 56
BtsI GCAGTG_nn'
Cuts at: 3031 3825 3845 3031
Size: 794 20 4701
Fragments arranged by size:
4701 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5515
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5480
DraI TTT'AAA
Cuts at: 2819 2819
Size: 5515
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4522 809
Size: 507 3206 1802
Fragments arranged by size:
3206 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2173 2188 2271
Size: 54 1425 50 275 18 343 15 83
Cuts at: 2271 2391 2787 2962 3851 5480 8
Size: 120 396 175 889 1629 43
Fragments arranged by size:
1629 1425 889 396 343 275 175 120
83 54 50 43 18 15
EagI C'GGCC_G
Cuts at: 1537 1812 2173 2391 2962 1537
Size: 275 361 218 571 4090
Fragments arranged by size:
4090 571 361 275 218
EarI CTCTTCn'nnn_
Cuts at: 1353 3445 1353
Size: 2092 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2305 4102 4476 4622 387
Size: 1064 138 716 1797 374 146 1280
Fragments arranged by size:
1797 1280 1064 716 374 146 138
EcoICRI GAG'CTC
Cuts at: 2010 2010
Size: 5515
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2362 2648 2654 3578 4175 4962 5230
Size: 393 730 286 6 924 597 787 268
Cuts at: 5230 1239
Size: 1524
Fragments arranged by size:
1524 924 787 730 597 393 286 268
6
EcoO109I rG'GnC_Cy
Cuts at: 2595 2802 2595
Size: 207 5308
EcoRI G'AATT_C
Cuts at: 2824 3202 2824
Size: 378 5137
FspI TGC'GCA
Cuts at: 2490 2546 4021 2490
Size: 56 1475 3984
Fragments arranged by size:
3984 1475 56
HaeII r_GCGC'y
Cuts at: 1678 2235 2246 2388 2513 4662 1678
Size: 557 11 142 125 2149 2531
Fragments arranged by size:
2531 2149 557 142 125 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4156 4188 4230 4262 1373
Size: 32 135 32 2584 32 42 32 2626
Fragments arranged by size:
2626 2584 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2859 3105 669
Size: 988 281 921 246 3079
Fragments arranged by size:
3079 988 921 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5515
HpaI GTT'AAC
Cuts at: 3105 3105
Size: 5515
KasI G'GCGC_C
Cuts at: 2242 2384 2509 2242
Size: 142 125 5248
Fragments arranged by size:
5248 142 125
KpnI G_GTAC'C
Cuts at: 2840 2840
Size: 5515
MfeI C'AATT_G
Cuts at: 3114 3114
Size: 5515
MluI A'CGCG_T
Cuts at: 5391 5391
Size: 5515
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4629 4813 1305
Size: 3324 184 2007
Fragments arranged by size:
3324 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3693
Fragments arranged by size:
3693 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2073 2783 3473 3832 3991 518
Size: 648 907 710 690 359 159 2042
Fragments arranged by size:
2042 907 710 690 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2305 2382 2792 2931 3608 4756 5001 1606
Size: 699 77 410 139 677 1148 245 2120
Fragments arranged by size:
2120 1148 699 677 410 245 139 77
NaeI GCC'GGC
Cuts at: 2271 2401 2271
Size: 130 5385
NarI GG'CG_CC
Cuts at: 2243 2385 2510 2243
Size: 142 125 5248
Fragments arranged by size:
5248 142 125
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4963
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5515
NgoMIV G'CCGG_C
Cuts at: 2269 2399 2269
Size: 130 5385
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5515
NotI GC'GGCC_GC
Cuts at: 2962 2962
Size: 5515
NspI r_CATG'y
Cuts at: 1132 1229 2125 2696 2873 4418 1132
Size: 97 896 571 177 1545 2229
Fragments arranged by size:
2229 1545 896 571 177 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5515
PciI A'CATG_T
Cuts at: 4414 4414
Size: 5515
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2626 1108
Size: 1518 3997
PmeI GTTT'AAAC
Cuts at: 2819 2819
Size: 5515
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2627 2659 3584 3616 5097 5129 2627
Size: 32 925 32 1481 32 3013
Fragments arranged by size:
3013 1481 925 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5515
PsiI TTA'TAA
Cuts at: 3085 3085
Size: 5515
PspOMI G'GGCC_C
Cuts at: 2595 2595
Size: 5515
PstI C_TGCA'G
Cuts at: 830 2867 830
Size: 2037 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5515
SacI G_AGCT'C
Cuts at: 2012 2012
Size: 5515
SacII CC_GC'GG
Cuts at: 2793 2793
Size: 5515
SalI G'TCGA_C
Cuts at: 1936 2857 1936
Size: 921 4594
SbfI CC_TGCA'GG
Cuts at: 2867 2867
Size: 5515
ScaI AGT'ACT
Cuts at: 3763 5475 3763
Size: 1712 3803
SfcI C'TryA_G
Cuts at: 826 1045 2863 3998 4679 4870 826
Size: 219 1818 1135 681 191 1471
Fragments arranged by size:
1818 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2603 2603
Size: 5515
SfoI GGC'GCC
Cuts at: 2244 2386 2511 2244
Size: 142 125 5248
Fragments arranged by size:
5248 142 125
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5515
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5515
SmaI CCC'GGG
Cuts at: 1872 2842 1872
Size: 970 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3619 4520 4782 820
Size: 197 926 1676 901 262 1553
Fragments arranged by size:
1676 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3306 493
Size: 2813 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5515
SphI G_CATG'C
Cuts at: 1132 2125 2873 1132
Size: 993 748 3774
Fragments arranged by size:
3774 993 748
SspI AAT'ATT
Cuts at: 5 52 3439 5
Size: 47 3387 2081
Fragments arranged by size:
3387 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5515
StyI C'CwwG_G
Cuts at: 513 1065 2954 513
Size: 552 1889 3074
Fragments arranged by size:
3074 1889 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2423 3542 3559 3712 3897 4342 325
Size: 1255 843 1119 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1119 843 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3761 5473 96
Size: 274 80 33 51 1443 1784 1712 138
Fragments arranged by size:
1784 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2183 2215 2272 2304 5475 5507 2183
Size: 32 57 32 3171 32 2191
Fragments arranged by size:
3171 2191 57 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5515
XbaI T'CTAG_A
Cuts at: 2851 2851
Size: 5515
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5515
XmaI C'CCGG_G
Cuts at: 1870 2840 1870
Size: 970 4545
XmnI GAAnn'nnTTC
Cuts at: 2642 3644 2642
Size: 1002 4513
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3323 276
Size: 53 83 186 936 1789 2468
Fragments arranged by size:
2468 1789 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI
SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI
FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI
PspXI PsrI PvuII RsrII SanDI SapI SexAI SrfI
SwaI XcmI