Restriction map for
pFN21AB8921
(Circular) (Six-base) MAPSORT of: pFN21AB8921.seq Check: 2741 from: 1 to: 5506
pFN21AB8921 5506 bp
With 182 enzymes: SgfI *
August 16, 2010 14:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2411 2411
Size: 5506
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3316 278
Size: 53 83 186 936 1780 2468
Fragments arranged by size:
2468 1780 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2849 1937
Size: 912 4594
Acc65I G'GTAC_C
Cuts at: 2827 2827
Size: 5506
AclI AA'CG_TT
Cuts at: 1563 3633 4006 1563
Size: 2070 373 3063
Fragments arranged by size:
3063 2070 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2128 2647 3569 4953 5221 1632
Size: 496 519 922 1384 268 1917
Fragments arranged by size:
1917 1384 922 519 496 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5309
AflIII A'CryG_T
Cuts at: 1203 4405 5382 1203
Size: 3202 977 1327
Fragments arranged by size:
3202 1327 977
AgeI A'CCGG_T
Cuts at: 4337 4337
Size: 5506
AhdI GACnn_n'nnGTC
Cuts at: 4235 4235
Size: 5506
AleI CACnn'nnGTG
Cuts at: 1166 2777 1166
Size: 1611 3895
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5506
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2468 2500 2468
Size: 32 5474
AlwNI CAG_nnn'CTG
Cuts at: 3249 4821 3249
Size: 1572 3934
ApaI G_GGCC'C
Cuts at: 2057 2057
Size: 5506
ApaLI G'TGCA_C
Cuts at: 3563 4719 3563
Size: 1156 4350
ApoI r'AATT_y
Cuts at: 1493 2326 2815 3041 3193 5302 1493
Size: 833 489 226 152 2109 1697
Fragments arranged by size:
2109 1697 833 489 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4060 160
Size: 872 3028 1606
Fragments arranged by size:
3028 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5506
AvaI C'yCGr_G
Cuts at: 1870 1943 2080 2262 2279 2831 1870
Size: 73 137 182 17 552 4545
Fragments arranged by size:
4545 552 182 137 73 17
BamHI G'GATC_C
Cuts at: 2836 3203 2836
Size: 367 5139
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2093 2827 4282 618
Size: 325 217 627 306 734 1455 1842
Fragments arranged by size:
1842 1455 734 627 325 306 217
BanII G_rGCy'C
Cuts at: 1391 2057 2396 2825 1391
Size: 666 339 429 4072
Fragments arranged by size:
4072 666 429 339
BbeI G_GCGC'C
Cuts at: 2097 2097
Size: 5506
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4535
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2243 2277 2584 2618 3697 3731 1903
Size: 34 306 34 307 34 1079 34 3678
Fragments arranged by size:
3678 1079 307 306 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3398 4608 3398
Size: 1210 4296
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5506
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2102 4117 136
Size: 107 122 71 631 605 430 2015 1525
Fragments arranged by size:
2015 1525 631 605 430 122 107 71
BglII A'GATC_T
Cuts at: 5501 5501
Size: 5506
BlpI GC'TnA_GC
Cuts at: 2923 2923
Size: 5506
Bme1580I G_kGCm'C
Cuts at: 1790 2057 2256 3567 4723 1790
Size: 267 199 1311 1156 2573
Fragments arranged by size:
2573 1311 1156 267 199
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5506
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3237 4195 442
Size: 416 53 83 399 24 1820 958 1753
Fragments arranged by size:
1820 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5506
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2326 2326
Size: 5506
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2116 2495 2515 2641 4166 1239
Size: 877 379 20 126 1525 2579
Fragments arranged by size:
2579 1525 877 379 126 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3631 4496 4794 3631
Size: 865 298 4343
Fragments arranged by size:
4343 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2134 2297 2546 4169 882
Size: 617 635 163 249 1623 2219
Fragments arranged by size:
2219 1623 635 617 249 163
BsaAI yAC'GTr
Cuts at: 493 1204 3297 493
Size: 711 2093 2702
Fragments arranged by size:
2702 2093 711
BsaBI GATnn'nnATC
Cuts at: 5500 5500
Size: 5506
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2094 2189 3313 3695
Size: 53 83 186 936 561 95 1124 382
Cuts at: 3695 4330 275
Size: 635 1451
Fragments arranged by size:
1451 1124 936 635 561 382 186 95
83 53
BsaWI w'CCGG_w
Cuts at: 3939 4337 4611 4758 5169 3939
Size: 398 274 147 411 4276
Fragments arranged by size:
4276 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2468 2498 1178
Size: 30 165 30 1065 30 4186
Fragments arranged by size:
4186 1065 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2063 2126 2456 1186
Size: 877 63 330 4236
Fragments arranged by size:
4236 877 330 63
BseYI C'CCAG_C
Cuts at: 2056 2460 4709 5205 2056
Size: 404 2249 496 2357
Fragments arranged by size:
2357 2249 496 404
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2389 2541 1163
Size: 573 653 152 4128
Fragments arranged by size:
4128 653 573 152
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2205 2220 2365 2956 3717 4745
Size: 275 186 204 15 145 591 761 1028
Cuts at: 4745 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 591 275 204 186 145
15
BsiHKAI G_wGCw'C
Cuts at: 3567 3652 4723 3567
Size: 85 1071 4350
Fragments arranged by size:
4350 1071 85
BsmI GAATG_Cn'
Cuts at: 3017 3110 3017
Size: 93 5413
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2057 2256 2396 2825 3567 3652 4723
Size: 399 267 199 140 429 742 85 1071
Cuts at: 4723 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 429 399 267 199 140
85
BspEI T'CCGG_A
Cuts at: 5169 5169
Size: 5506
BspHI T'CATG_A
Cuts at: 3395 5125 3395
Size: 1730 3776
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2411 2861 844
Size: 1567 450 3489
Fragments arranged by size:
3489 1567 450
BsrBI CCG'CTC
Cuts at: 1394 1823 2342 3393 1394
Size: 429 519 1051 3507
Fragments arranged by size:
3507 1051 519 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2032 2508 4001 4175 65
Size: 1183 784 476 1493 174 1396
Fragments arranged by size:
1493 1396 1183 784 476 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2200 4150 4337 1700
Size: 110 390 1950 187 2869
Fragments arranged by size:
2869 1950 390 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5506
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5506
BssSI C'ACGA_G
Cuts at: 3566 4578 3566
Size: 1012 4494
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2519 794
Size: 1725 3781
BstBI TT'CG_AA
Cuts at: 4310 4310
Size: 5506
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5506
BstXI CCAn_nnnn'nTGG
Cuts at: 2779 2779
Size: 5506
BstYI r'GATC_y
Cuts at: 1972 2836 3203 3588 3605 5057 5143 5155 5501
Size: 864 367 385 17 1452 86 12 346
Cuts at: 5501 1972
Size: 1977
Fragments arranged by size:
1977 1452 864 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2411 2411
Size: 5506
BtgI C'CryG_G
Cuts at: 513 1065 2004 2106 513
Size: 552 939 102 3913
Fragments arranged by size:
3913 939 552 102
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4767
Fragments arranged by size:
4767 683 56
BtsI GCAGTG_nn'
Cuts at: 2067 2237 3022 3816 3836 2067
Size: 170 785 794 20 3737
Fragments arranged by size:
3737 794 785 170 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5506
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5471
DraI TTT'AAA
Cuts at: 2810 2810
Size: 5506
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4513 809
Size: 507 3197 1802
Fragments arranged by size:
3197 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2067 2202 2217
Size: 54 1425 50 275 18 237 135 15
Cuts at: 2217 2234 2362 2953 3842 5471 8
Size: 17 128 591 889 1629 43
Fragments arranged by size:
1629 1425 889 591 275 237 135 128
54 50 43 18 17 15
EagI C'GGCC_G
Cuts at: 1537 1812 2202 2217 2362 2953 1537
Size: 275 390 15 145 591 4090
Fragments arranged by size:
4090 591 390 275 145 15
EarI CTCTTCn'nnn_
Cuts at: 1353 3436 1353
Size: 2083 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4093 4467 4613 387
Size: 1064 138 2504 374 146 1280
Fragments arranged by size:
2504 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2116 2128 2495 2515 2641 2647 3569
Size: 393 484 12 367 20 126 6 922
Cuts at: 3569 4166 4953 5221 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 922 787 597 484 393 367 268
126 20 12 6
EcoO109I rG'GnC_Cy
Cuts at: 2053 2084 2053
Size: 31 5475
EcoRI G'AATT_C
Cuts at: 2815 3193 2815
Size: 378 5128
FspI TGC'GCA
Cuts at: 4012 4012
Size: 5506
HaeII r_GCGC'y
Cuts at: 1678 2097 2119 2159 2214 2262 4653 1678
Size: 419 22 40 55 48 2391 2531
Fragments arranged by size:
2531 2391 419 55 48 40 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4147 4179 4221 4253 1373
Size: 32 135 32 2575 32 42 32 2626
Fragments arranged by size:
2626 2575 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2850 3096 669
Size: 988 281 912 246 3079
Fragments arranged by size:
3079 988 912 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5506
HpaI GTT'AAC
Cuts at: 3096 3096
Size: 5506
KasI G'GCGC_C
Cuts at: 2093 2093
Size: 5506
KpnI G_GTAC'C
Cuts at: 2831 2831
Size: 5506
MfeI C'AATT_G
Cuts at: 3105 3105
Size: 5506
MluI A'CGCG_T
Cuts at: 5382 5382
Size: 5506
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2190 2301 4620 4804 1305
Size: 885 111 2319 184 2007
Fragments arranged by size:
2319 2007 885 184 111
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3684
Fragments arranged by size:
3684 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2777 3464 3823 3982 518
Size: 648 1611 687 359 159 2042
Fragments arranged by size:
2042 1611 687 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2108 2197 2287 2922 3599 4747 4992 1606
Size: 502 89 90 635 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 635 502 245 90 89
NaeI GCC'GGC
Cuts at: 2202 2202
Size: 5506
NarI GG'CG_CC
Cuts at: 2094 2094
Size: 5506
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4015
Fragments arranged by size:
4015 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5506
NgoMIV G'CCGG_C
Cuts at: 2200 2200
Size: 5506
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5506
NotI GC'GGCC_GC
Cuts at: 2953 2953
Size: 5506
NspI r_CATG'y
Cuts at: 1132 1229 2794 2864 4409 1132
Size: 97 1565 70 1545 2229
Fragments arranged by size:
2229 1565 1545 97 70
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5506
PciI A'CATG_T
Cuts at: 4405 4405
Size: 5506
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5506
PmeI GTTT'AAAC
Cuts at: 2810 2810
Size: 5506
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2468 2500 3575 3607 5088 5120 2468
Size: 32 1075 32 1481 32 2854
Fragments arranged by size:
2854 1481 1075 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2558 1613
Size: 945 4561
PsiI TTA'TAA
Cuts at: 3076 3076
Size: 5506
PspOMI G'GGCC_C
Cuts at: 2053 2053
Size: 5506
PstI C_TGCA'G
Cuts at: 830 2607 2697 2858 830
Size: 1777 90 161 3478
Fragments arranged by size:
3478 1777 161 90
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5506
SacII CC_GC'GG
Cuts at: 2109 2109
Size: 5506
SalI G'TCGA_C
Cuts at: 1936 2848 1936
Size: 912 4594
SbfI CC_TGCA'GG
Cuts at: 2697 2858 2697
Size: 161 5345
ScaI AGT'ACT
Cuts at: 3754 5466 3754
Size: 1712 3794
SfcI C'TryA_G
Cuts at: 826 1045 2603 2693 2854 3989 4670 4861 826
Size: 219 1558 90 161 1135 681 191 1471
Fragments arranged by size:
1558 1471 1135 681 219 191 161 90
SfoI GGC'GCC
Cuts at: 2095 2095
Size: 5506
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5506
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5506
SmaI CCC'GGG
Cuts at: 1872 2082 2281 2833 1872
Size: 210 199 552 4545
Fragments arranged by size:
4545 552 210 199
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3610 4511 4773 820
Size: 197 926 1667 901 262 1553
Fragments arranged by size:
1667 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3297 493
Size: 2804 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5506
SphI G_CATG'C
Cuts at: 1132 2864 1132
Size: 1732 3774
SspI AAT'ATT
Cuts at: 5 52 3430 5
Size: 47 3378 2081
Fragments arranged by size:
3378 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5506
StyI C'CwwG_G
Cuts at: 513 1065 2004 2181 2589 2945 513
Size: 552 939 177 408 356 3074
Fragments arranged by size:
3074 939 552 408 356 177
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2302 3533 3550 3703 3888 4333 325
Size: 1255 722 1231 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1231 722 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3752 5464 96
Size: 274 80 33 51 1443 1775 1712 138
Fragments arranged by size:
1775 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5466 5498 5466
Size: 32 5474
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5506
XbaI T'CTAG_A
Cuts at: 2842 2842
Size: 5506
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2755 2755
Size: 5506
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5506
XmaI C'CCGG_G
Cuts at: 1870 2080 2279 2831 1870
Size: 210 199 552 4545
Fragments arranged by size:
4545 552 210 199
XmnI GAAnn'nnTTC
Cuts at: 2297 3635 2297
Size: 1338 4168
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3314 276
Size: 53 83 186 936 1780 2468
Fragments arranged by size:
2468 1780 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI
BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI
FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI
PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI
SrfI SwaI