Restriction map for
pFN21AB8920
(Circular) (Six-base) MAPSORT of: pFN21AB8920.seq Check: 6689 from: 1 to: 5506
pFN21AB8920 5506 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2325 3316 278
Size: 53 83 186 936 789 991 2468
Fragments arranged by size:
2468 991 936 789 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2849 1937
Size: 912 4594
Acc65I G'GTAC_C
Cuts at: 2827 2827
Size: 5506
AclI AA'CG_TT
Cuts at: 1563 3633 4006 1563
Size: 2070 373 3063
Fragments arranged by size:
3063 2070 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2042 3569 4953 5221 1632
Size: 410 1527 1384 268 1917
Fragments arranged by size:
1917 1527 1384 410 268
AfeI AGC'GCT
Cuts at: 2414 2711 2414
Size: 297 5209
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5309
AflIII A'CryG_T
Cuts at: 1203 4405 5382 1203
Size: 3202 977 1327
Fragments arranged by size:
3202 1327 977
AgeI A'CCGG_T
Cuts at: 4337 4337
Size: 5506
AhdI GACnn_n'nnGTC
Cuts at: 4235 4235
Size: 5506
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5506
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5506
AlwNI CAG_nnn'CTG
Cuts at: 2212 3249 4821 2212
Size: 1037 1572 2897
Fragments arranged by size:
2897 1572 1037
ApaI G_GGCC'C
Cuts at: 2038 2399 2608 2663 2038
Size: 361 209 55 4881
Fragments arranged by size:
4881 361 209 55
ApaLI G'TGCA_C
Cuts at: 3563 4719 3563
Size: 1156 4350
ApoI r'AATT_y
Cuts at: 1493 2815 3041 3193 5302 1493
Size: 1322 226 152 2109 1697
Fragments arranged by size:
2109 1697 1322 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4060 160
Size: 872 3028 1606
Fragments arranged by size:
3028 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5506
AvaI C'yCGr_G
Cuts at: 1870 1943 2029 2188 2366 2831 1870
Size: 73 86 159 178 465 4545
Fragments arranged by size:
4545 465 178 159 86 73
BamHI G'GATC_C
Cuts at: 2836 3203 2836
Size: 367 5139
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2085 2384 2466 2492 2827
Size: 325 217 627 298 299 82 26 335
Cuts at: 2827 4282 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 335 325 299 298 217
82 26
BanII G_rGCy'C
Cuts at: 1391 2038 2373 2399 2608 2663 2825 1391
Size: 647 335 26 209 55 162 4072
Fragments arranged by size:
4072 647 335 209 162 55 26
BbeI G_GCGC'C
Cuts at: 2470 2496 2470
Size: 26 5480
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2343 928
Size: 971 444 4091
Fragments arranged by size:
4091 971 444
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2567 2601 3697 3731 1903
Size: 34 50 34 546 34 1096 34 3678
Fragments arranged by size:
3678 1096 546 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3398 4608 3398
Size: 1210 4296
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5506
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2580 4117 136
Size: 107 122 71 631 605 908 1537 1525
Fragments arranged by size:
1537 1525 908 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5501 5501
Size: 5506
BlpI GC'TnA_GC
Cuts at: 2923 2923
Size: 5506
Bme1580I G_kGCm'C
Cuts at: 1790 2038 2399 2608 2663 3567 4723 1790
Size: 248 361 209 55 904 1156 2573
Fragments arranged by size:
2573 1156 904 361 248 209 55
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5506
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2465 3237 4195
Size: 416 53 83 399 24 1048 772 958
Cuts at: 4195 442
Size: 1753
Fragments arranged by size:
1753 1048 958 772 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5506
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2341 2341
Size: 5506
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2111 2156 2546 2639 4166 1239
Size: 872 45 390 93 1527 2579
Fragments arranged by size:
2579 1527 872 390 93 45
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3631 4496 4794 3631
Size: 865 298 4343
Fragments arranged by size:
4343 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4169 882
Size: 617 2670 2219
Fragments arranged by size:
2670 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3297 493
Size: 711 2093 2702
Fragments arranged by size:
2702 2093 711
BsaBI GATnn'nnATC
Cuts at: 5500 5500
Size: 5506
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2322 2467 2493 2747
Size: 53 83 186 936 789 145 26 254
Cuts at: 2747 3313 3695 4330 275
Size: 566 382 635 1451
Fragments arranged by size:
1451 936 789 635 566 382 254 186
145 83 53 26
BsaWI w'CCGG_w
Cuts at: 3939 4337 4611 4758 5169 3939
Size: 398 274 147 411 4276
Fragments arranged by size:
4276 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5281
Fragments arranged by size:
5281 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5506
BseYI C'CCAG_C
Cuts at: 2253 2439 2685 4709 5205 2253
Size: 186 246 2024 496 2554
Fragments arranged by size:
2554 2024 496 246 186
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4933
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2172 2269 2511 2643 2956 3717
Size: 275 186 171 97 242 132 313 761
Cuts at: 3717 4745 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 761 313 275 242 186 171
132 97
BsiHKAI G_wGCw'C
Cuts at: 2433 2625 2679 3567 3652 4723 2433
Size: 192 54 888 85 1071 3216
Fragments arranged by size:
3216 1071 888 192 85 54
BsmI GAATG_Cn'
Cuts at: 3017 3110 3017
Size: 93 5413
BsmBI CGTCTCn'nnnn_
Cuts at: 2329 2329
Size: 5506
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2038 2373 2399 2433 2608 2625 2663
Size: 399 248 335 26 34 175 17 38
Cuts at: 2663 2679 2825 3567 3652 4723 1391
Size: 16 146 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 335 248 175 146
85 38 34 26 17 16
BspEI T'CCGG_A
Cuts at: 5169 5169
Size: 5506
BspHI T'CATG_A
Cuts at: 2724 3395 5125 2724
Size: 671 1730 3105
Fragments arranged by size:
3105 1730 671
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2139 2861 844
Size: 1295 722 3489
Fragments arranged by size:
3489 1295 722
BsrBI CCG'CTC
Cuts at: 1394 1823 2028 3393 1394
Size: 429 205 1365 3507
Fragments arranged by size:
3507 1365 429 205
BsrDI GCAATG_nn'
Cuts at: 65 1248 4001 4175 65
Size: 1183 2753 174 1396
Fragments arranged by size:
2753 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2449 4150 4337 1700
Size: 110 639 1701 187 2869
Fragments arranged by size:
2869 1701 639 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5506
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5506
BssSI C'ACGA_G
Cuts at: 3566 4578 3566
Size: 1012 4494
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2629 794
Size: 1835 3671
BstBI TT'CG_AA
Cuts at: 4310 4310
Size: 5506
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5506
BstYI r'GATC_y
Cuts at: 1972 2836 3203 3588 3605 5057 5143 5155 5501
Size: 864 367 385 17 1452 86 12 346
Cuts at: 5501 1972
Size: 1977
Fragments arranged by size:
1977 1452 864 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2355 2355
Size: 5506
BtgI C'CryG_G
Cuts at: 513 1065 2067 2118 2424 513
Size: 552 1002 51 306 3595
Fragments arranged by size:
3595 1002 552 306 51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2394 487
Size: 683 56 1168 3599
Fragments arranged by size:
3599 1168 683 56
BtsI GCAGTG_nn'
Cuts at: 3022 3816 3836 3022
Size: 794 20 4692
Fragments arranged by size:
4692 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5506
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5471
DraI TTT'AAA
Cuts at: 2810 2810
Size: 5506
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4513 809
Size: 507 3197 1802
Fragments arranged by size:
3197 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2169 2266 2508
Size: 54 1425 50 275 18 339 97 242
Cuts at: 2508 2548 2640 2953 3842 5471 8
Size: 40 92 313 889 1629 43
Fragments arranged by size:
1629 1425 889 339 313 275 242 97
92 54 50 43 40 18
EagI C'GGCC_G
Cuts at: 1537 1812 2169 2266 2508 2640 2953 1537
Size: 275 357 97 242 132 313 4090
Fragments arranged by size:
4090 357 313 275 242 132 97
EarI CTCTTCn'nnn_
Cuts at: 1353 2732 3436 1353
Size: 1379 704 3423
Fragments arranged by size:
3423 1379 704
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4093 4467 4613 387
Size: 1064 138 2504 374 146 1280
Fragments arranged by size:
2504 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2042 2111 2156 2546 2639 3569 4166
Size: 393 410 69 45 390 93 930 597
Cuts at: 4166 4953 5221 1239
Size: 787 268 1524
Fragments arranged by size:
1524 930 787 597 410 393 390 268
93 69 45
EcoO109I rG'GnC_Cy
Cuts at: 2034 2035 2110 2604 2659 2660 2034
Size: 1 75 494 55 1 4880
Fragments arranged by size:
4880 494 75 55 1 1
EcoRI G'AATT_C
Cuts at: 2815 3193 2815
Size: 378 5128
FspI TGC'GCA
Cuts at: 4012 4012
Size: 5506
HaeII r_GCGC'y
Cuts at: 1678 2416 2470 2496 2713 4653 1678
Size: 738 54 26 217 1940 2531
Fragments arranged by size:
2531 1940 738 217 54 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2489 2521 4147 4179 4221
Size: 32 135 32 917 32 1626 32 42
Cuts at: 4221 4253 1373
Size: 32 2626
Fragments arranged by size:
2626 1626 917 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2850 3096 669
Size: 988 281 912 246 3079
Fragments arranged by size:
3079 988 912 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5506
HpaI GTT'AAC
Cuts at: 3096 3096
Size: 5506
KasI G'GCGC_C
Cuts at: 2466 2492 2466
Size: 26 5480
KpnI G_GTAC'C
Cuts at: 2831 2831
Size: 5506
MfeI C'AATT_G
Cuts at: 3105 3105
Size: 5506
MluI A'CGCG_T
Cuts at: 5382 5382
Size: 5506
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4620 4804 1305
Size: 3315 184 2007
Fragments arranged by size:
3315 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2550 10
Size: 54 1425 343 718 2966
Fragments arranged by size:
2966 1425 718 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2072 3464 3823 3982 518
Size: 648 906 1392 359 159 2042
Fragments arranged by size:
2042 1392 906 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2253 2289 2298 2330 2350 2439 2685 2922
Size: 647 36 9 32 20 89 246 237
Cuts at: 2922 3599 4747 4992 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 647 246 245 237 89
36 32 20 9
NarI GG'CG_CC
Cuts at: 2467 2493 2467
Size: 26 5480
NcoI C'CATG_G
Cuts at: 513 1065 2067 2424 513
Size: 552 1002 357 3595
Fragments arranged by size:
3595 1002 552 357
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5506
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5506
NotI GC'GGCC_GC
Cuts at: 2953 2953
Size: 5506
NspI r_CATG'y
Cuts at: 1132 1229 2864 4409 1132
Size: 97 1635 1545 2229
Fragments arranged by size:
2229 1635 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5506
PciI A'CATG_T
Cuts at: 4405 4405
Size: 5506
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5506
PfoI T'CCnGG_A
Cuts at: 2559 2559
Size: 5506
PmeI GTTT'AAAC
Cuts at: 2810 2810
Size: 5506
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3575 3607 5088 5120 3575
Size: 32 1481 32 3961
Fragments arranged by size:
3961 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2110 2110
Size: 5506
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5506
PsiI TTA'TAA
Cuts at: 3076 3076
Size: 5506
PspOMI G'GGCC_C
Cuts at: 2034 2395 2604 2659 2034
Size: 361 209 55 4881
Fragments arranged by size:
4881 361 209 55
PstI C_TGCA'G
Cuts at: 830 2024 2858 830
Size: 1194 834 3478
Fragments arranged by size:
3478 1194 834
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5506
SalI G'TCGA_C
Cuts at: 1936 2848 1936
Size: 912 4594
SapI GCTCTTCn'nnn_
Cuts at: 2732 2732
Size: 5506
SbfI CC_TGCA'GG
Cuts at: 2858 2858
Size: 5506
ScaI AGT'ACT
Cuts at: 3754 5466 3754
Size: 1712 3794
SfcI C'TryA_G
Cuts at: 826 1045 2020 2665 2854 3989 4670 4861 826
Size: 219 975 645 189 1135 681 191 1471
Fragments arranged by size:
1471 1135 975 681 645 219 191 189
SfiI GGCCn_nnn'nGGCC
Cuts at: 2580 2580
Size: 5506
SfoI GGC'GCC
Cuts at: 2468 2494 2468
Size: 26 5480
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5506
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5506
SmaI CCC'GGG
Cuts at: 1872 2190 2833 1872
Size: 318 643 4545
Fragments arranged by size:
4545 643 318
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3610 4511 4773 820
Size: 197 926 1667 901 262 1553
Fragments arranged by size:
1667 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3297 493
Size: 2804 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5506
SphI G_CATG'C
Cuts at: 1132 2864 1132
Size: 1732 3774
SspI AAT'ATT
Cuts at: 5 52 3430 5
Size: 47 3378 2081
Fragments arranged by size:
3378 2081 47
StuI AGG'CCT
Cuts at: 1517 2063 2516 1517
Size: 546 453 4507
Fragments arranged by size:
4507 546 453
StyI C'CwwG_G
Cuts at: 513 1065 2067 2424 2700 2945 513
Size: 552 1002 357 276 245 3074
Fragments arranged by size:
3074 1002 552 357 276 245
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2231 2274 3533 3550 3703 3888 4333
Size: 1255 651 43 1259 17 153 185 445
Cuts at: 4333 325
Size: 1498
Fragments arranged by size:
1498 1259 1255 651 445 185 153 43
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3752 5464 96
Size: 274 80 33 51 1443 1775 1712 138
Fragments arranged by size:
1775 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5466 5498 5466
Size: 32 5474
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5506
XbaI T'CTAG_A
Cuts at: 2842 2842
Size: 5506
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5506
XmaI C'CCGG_G
Cuts at: 1870 2188 2831 1870
Size: 318 643 4545
Fragments arranged by size:
4545 643 318
XmnI GAAnn'nnTTC
Cuts at: 3635 3635
Size: 5506
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2323 3314 276
Size: 53 83 186 936 789 991 2468
Fragments arranged by size:
2468 991 936 789 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI
PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI
SrfI SwaI XcmI