Restriction map for
pFN21AB8919
(Circular) (Six-base) MAPSORT of: pFN21AB8919.seq Check: 2897 from: 1 to: 5497
pFN21AB8919 5497 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3307 278
Size: 53 83 186 936 1771 2468
Fragments arranged by size:
2468 1771 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2409 2840 1937
Size: 472 431 4594
Fragments arranged by size:
4594 472 431
Acc65I G'GTAC_C
Cuts at: 2818 2818
Size: 5497
AclI AA'CG_TT
Cuts at: 1563 3624 3997 1563
Size: 2061 373 3063
Fragments arranged by size:
3063 2061 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2754 3560 4944 5212 1632
Size: 1122 806 1384 268 1917
Fragments arranged by size:
1917 1384 1122 806 268
AfeI AGC'GCT
Cuts at: 2477 2477
Size: 5497
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5300
AflIII A'CryG_T
Cuts at: 1203 4396 5373 1203
Size: 3193 977 1327
Fragments arranged by size:
3193 1327 977
AgeI A'CCGG_T
Cuts at: 4328 4328
Size: 5497
AhdI GACnn_n'nnGTC
Cuts at: 4226 4226
Size: 5497
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5497
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5497
AlwNI CAG_nnn'CTG
Cuts at: 2158 3240 4812 2158
Size: 1082 1572 2843
Fragments arranged by size:
2843 1572 1082
ApaI G_GGCC'C
Cuts at: 2229 2229
Size: 5497
ApaLI G'TGCA_C
Cuts at: 3554 4710 3554
Size: 1156 4341
ApoI r'AATT_y
Cuts at: 1493 2051 2427 2806 3032 3184 5293 1493
Size: 558 376 379 226 152 2109 1697
Fragments arranged by size:
2109 1697 558 379 376 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4051 160
Size: 872 3019 1606
Fragments arranged by size:
3019 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5497
AvaI C'yCGr_G
Cuts at: 1870 1943 2276 2479 2722 2822 1870
Size: 73 333 203 243 100 4545
Fragments arranged by size:
4545 333 243 203 100 73
BamHI G'GATC_C
Cuts at: 2827 3194 2827
Size: 367 5130
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2305 2318 2818 4273 618
Size: 325 217 627 518 13 500 1455 1842
Fragments arranged by size:
1842 1455 627 518 500 325 217 13
BanII G_rGCy'C
Cuts at: 1391 2018 2229 2816 1391
Size: 627 211 587 4072
Fragments arranged by size:
4072 627 587 211
BbeI G_GCGC'C
Cuts at: 2309 2322 2309
Size: 13 5484
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2742 928
Size: 971 843 3683
Fragments arranged by size:
3683 971 843
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3688 3722 1903
Size: 34 1751 34 3678
Fragments arranged by size:
3678 1751 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3389 4599 3389
Size: 1210 4287
BclI T'GATC_A
Cuts at: 1548 2007 1548
Size: 459 5038
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4108 136
Size: 107 122 71 631 605 2436 1525
Fragments arranged by size:
2436 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5492 5492
Size: 5497
BlpI GC'TnA_GC
Cuts at: 2914 2914
Size: 5497
Bme1580I G_kGCm'C
Cuts at: 1790 2229 3558 4714 1790
Size: 439 1329 1156 2573
Fragments arranged by size:
2573 1329 1156 439
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5497
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3228 4186 442
Size: 416 53 83 399 24 1811 958 1753
Fragments arranged by size:
1811 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5497
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2204 2365 4157 1239
Size: 965 161 1792 2579
Fragments arranged by size:
2579 1792 965 161
Bpu10I CC'TnA_GC
Cuts at: 2193 2193
Size: 5497
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3622 4487 4785 3622
Size: 865 298 4334
Fragments arranged by size:
4334 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4160 882
Size: 617 2661 2219
Fragments arranged by size:
2661 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3288 493
Size: 711 2084 2702
Fragments arranged by size:
2702 2084 711
BsaBI GATnn'nnATC
Cuts at: 2567 2606 5491 2567
Size: 39 2885 2573
Fragments arranged by size:
2885 2573 39
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2306 2319 3304 3686
Size: 53 83 186 936 773 13 985 382
Cuts at: 3686 4321 275
Size: 635 1451
Fragments arranged by size:
1451 985 936 773 635 382 186 83
53 13
BsaWI w'CCGG_w
Cuts at: 2104 3930 4328 4602 4749 5160 2104
Size: 1826 398 274 147 411 2441
Fragments arranged by size:
2441 1826 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2363 2393 1178
Size: 30 165 30 960 30 4282
Fragments arranged by size:
4282 960 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5497
BseYI C'CCAG_C
Cuts at: 2338 2699 4700 5196 2338
Size: 361 2001 496 2639
Fragments arranged by size:
2639 2001 496 361
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2194 1163
Size: 573 458 4466
Fragments arranged by size:
4466 573 458
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2119 2688 2947 3708 4736 1540
Size: 275 186 118 569 259 761 1028 2301
Fragments arranged by size:
2301 1028 761 569 275 259 186 118
BsiHKAI G_wGCw'C
Cuts at: 2018 3558 3643 4714 2018
Size: 1540 85 1071 2801
Fragments arranged by size:
2801 1540 1071 85
BsmI GAATG_Cn'
Cuts at: 3008 3101 3008
Size: 93 5404
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2018 2229 2816 3558 3643 4714 1391
Size: 399 228 211 587 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 587 399 228 211 85
BspEI T'CCGG_A
Cuts at: 2104 5160 2104
Size: 3056 2441
BspHI T'CATG_A
Cuts at: 2010 3386 5116 2010
Size: 1376 1730 2391
Fragments arranged by size:
2391 1730 1376
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2852 844
Size: 2008 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3384 1394
Size: 429 1561 3507
Fragments arranged by size:
3507 1561 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3992 4166 65
Size: 1183 2744 174 1396
Fragments arranged by size:
2744 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2251 2302 2687 4141 4328 1700
Size: 110 441 51 385 1454 187 2869
Fragments arranged by size:
2869 1454 441 385 187 110 51
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5497
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5497
BssSI C'ACGA_G
Cuts at: 3557 4569 3557
Size: 1012 4485
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5497
BstBI TT'CG_AA
Cuts at: 4301 4301
Size: 5497
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5497
BstYI r'GATC_y
Cuts at: 1972 2827 3194 3579 3596 5048 5134 5146 5492
Size: 855 367 385 17 1452 86 12 346
Cuts at: 5492 1972
Size: 1977
Fragments arranged by size:
1977 1452 855 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2245 2414 513
Size: 552 1180 169 3596
Fragments arranged by size:
3596 1180 552 169
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4758
Fragments arranged by size:
4758 683 56
BtsI GCAGTG_nn'
Cuts at: 3013 3807 3827 3013
Size: 794 20 4683
Fragments arranged by size:
4683 794 20
ClaI AT'CG_AT
Cuts at: 1554 2043 1554
Size: 489 5008
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5462
DraI TTT'AAA
Cuts at: 2801 2801
Size: 5497
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4504 809
Size: 507 3188 1802
Fragments arranged by size:
3188 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2116 2685 2944
Size: 54 1425 50 275 18 286 569 259
Cuts at: 2944 3833 5462 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 569 286 275 259 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2116 2685 2944 1537
Size: 275 304 569 259 4090
Fragments arranged by size:
4090 569 304 275 259
EarI CTCTTCn'nnn_
Cuts at: 1353 3427 1353
Size: 2074 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2314 4084 4458 4604 387
Size: 1064 138 725 1770 374 146 1280
Fragments arranged by size:
1770 1280 1064 725 374 146 138
EcoICRI GAG'CTC
Cuts at: 2016 2016
Size: 5497
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2204 2365 2754 3560 4157 4944 5212
Size: 393 572 161 389 806 597 787 268
Cuts at: 5212 1239
Size: 1524
Fragments arranged by size:
1524 806 787 597 572 393 389 268
161
EcoNI CCTnn'n_nnAGG
Cuts at: 2313 2313
Size: 5497
EcoO109I rG'GnC_Cy
Cuts at: 2225 2225
Size: 5497
EcoRI G'AATT_C
Cuts at: 2806 3184 2806
Size: 378 5119
FseI GG_CCGG'CC
Cuts at: 2255 2255
Size: 5497
FspI TGC'GCA
Cuts at: 4003 4003
Size: 5497
HaeII r_GCGC'y
Cuts at: 1678 2200 2309 2322 2388 2479 2694 2754 4644
Size: 522 109 13 66 91 215 60 1890
Cuts at: 4644 1678
Size: 2531
Fragments arranged by size:
2531 1890 522 215 109 91 66 60
13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2363 2395 4138 4170 4212
Size: 32 135 32 791 32 1743 32 42
Cuts at: 4212 4244 1373
Size: 32 2626
Fragments arranged by size:
2626 1743 791 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2841 3087 669
Size: 988 281 903 246 3079
Fragments arranged by size:
3079 988 903 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5497
HpaI GTT'AAC
Cuts at: 3087 3087
Size: 5497
KasI G'GCGC_C
Cuts at: 2305 2318 2305
Size: 13 5484
KpnI G_GTAC'C
Cuts at: 2822 2822
Size: 5497
MfeI C'AATT_G
Cuts at: 3096 3096
Size: 5497
MluI A'CGCG_T
Cuts at: 5373 5373
Size: 5497
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4611 4795 1305
Size: 3306 184 2007
Fragments arranged by size:
3306 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3675
Fragments arranged by size:
3675 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3455 3814 3973 518
Size: 648 843 1446 359 159 2042
Fragments arranged by size:
2042 1446 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2913 3590 4738 4983 1606
Size: 1307 677 1148 245 2120
Fragments arranged by size:
2120 1307 1148 677 245
NaeI GCC'GGC
Cuts at: 2253 2304 2689 2253
Size: 51 385 5061
Fragments arranged by size:
5061 385 51
NarI GG'CG_CC
Cuts at: 2306 2319 2306
Size: 13 5484
NcoI C'CATG_G
Cuts at: 513 1065 2414 513
Size: 552 1349 3596
Fragments arranged by size:
3596 1349 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5497
NgoMIV G'CCGG_C
Cuts at: 2251 2302 2687 2251
Size: 51 385 5061
Fragments arranged by size:
5061 385 51
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5497
NotI GC'GGCC_GC
Cuts at: 2944 2944
Size: 5497
NruI TCG'CGA
Cuts at: 2239 2239
Size: 5497
NspI r_CATG'y
Cuts at: 1132 1229 2855 4400 1132
Size: 97 1626 1545 2229
Fragments arranged by size:
2229 1626 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5497
PciI A'CATG_T
Cuts at: 4396 4396
Size: 5497
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5497
PfoI T'CCnGG_A
Cuts at: 2508 2508
Size: 5497
PmeI GTTT'AAAC
Cuts at: 2801 2801
Size: 5497
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3566 3598 5079 5111 3566
Size: 32 1481 32 3952
Fragments arranged by size:
3952 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5497
PsiI TTA'TAA
Cuts at: 2457 3067 2457
Size: 610 4887
PspOMI G'GGCC_C
Cuts at: 2225 2225
Size: 5497
PspXI vC'TCGA_Gb
Cuts at: 2479 2479
Size: 5497
PstI C_TGCA'G
Cuts at: 830 2357 2849 830
Size: 1527 492 3478
Fragments arranged by size:
3478 1527 492
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5497
SacI G_AGCT'C
Cuts at: 2018 2018
Size: 5497
SalI G'TCGA_C
Cuts at: 1936 2839 1936
Size: 903 4594
SbfI CC_TGCA'GG
Cuts at: 2849 2849
Size: 5497
ScaI AGT'ACT
Cuts at: 3745 5457 3745
Size: 1712 3785
SexAI A'CCwGG_T
Cuts at: 2789 2789
Size: 5497
SfcI C'TryA_G
Cuts at: 826 1045 2353 2845 3980 4661 4852 826
Size: 219 1308 492 1135 681 191 1471
Fragments arranged by size:
1471 1308 1135 681 492 219 191
SfoI GGC'GCC
Cuts at: 2307 2320 2307
Size: 13 5484
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5497
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5497
SmaI CCC'GGG
Cuts at: 1872 2724 2824 1872
Size: 852 100 4545
Fragments arranged by size:
4545 852 100
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2479 3601 4502 4764 820
Size: 197 926 536 1122 901 262 1553
Fragments arranged by size:
1553 1122 926 901 536 262 197
SnaBI TAC'GTA
Cuts at: 493 3288 493
Size: 2795 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5497
SphI G_CATG'C
Cuts at: 1132 2855 1132
Size: 1723 3774
SspI AAT'ATT
Cuts at: 5 52 2072 2111 3421 5
Size: 47 2020 39 1310 2081
Fragments arranged by size:
2081 2020 1310 47 39
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5497
StyI C'CwwG_G
Cuts at: 513 1065 2414 2936 513
Size: 552 1349 522 3074
Fragments arranged by size:
3074 1349 552 522
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3524 3541 3694 3879 4324 325
Size: 1255 1944 17 153 185 445 1498
Fragments arranged by size:
1944 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3743 5455 96
Size: 274 80 33 51 1443 1766 1712 138
Fragments arranged by size:
1766 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5457 5489 5457
Size: 32 5465
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5497
XbaI T'CTAG_A
Cuts at: 2833 2833
Size: 5497
XhoI C'TCGA_G
Cuts at: 1943 2479 1943
Size: 536 4961
XmaI C'CCGG_G
Cuts at: 1870 2722 2822 1870
Size: 852 100 4545
Fragments arranged by size:
4545 852 100
XmnI GAAnn'nnTTC
Cuts at: 3626 3626
Size: 5497
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3305 276
Size: 53 83 186 936 1771 2468
Fragments arranged by size:
2468 1771 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PspXI PstI PvuI SacI SalI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FalI
FspAI NsiI PacI PmlI PpuMI PsrI PvuII RsrII
SacII SanDI SapI SfiI SrfI SwaI XcmI