Restriction map for
pFN21AB8915
(Circular) (Six-base) MAPSORT of: pFN21AB8915.seq Check: 2397 from: 1 to: 5476
pFN21AB8915 5476 bp
With 182 enzymes: SgfI *
March 28, 2012 14:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2177 2583 2177
Size: 406 5070
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3286 278
Size: 53 83 186 936 1750 2468
Fragments arranged by size:
2468 1750 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2819 1937
Size: 882 4594
Acc65I G'GTAC_C
Cuts at: 2797 2797
Size: 5476
AclI AA'CG_TT
Cuts at: 1563 3603 3976 1563
Size: 2040 373 3063
Fragments arranged by size:
3063 2040 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3539 4923 5191 1632
Size: 1907 1384 268 1917
Fragments arranged by size:
1917 1907 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5279
AflIII A'CryG_T
Cuts at: 1203 4375 5352 1203
Size: 3172 977 1327
Fragments arranged by size:
3172 1327 977
AgeI A'CCGG_T
Cuts at: 4307 4307
Size: 5476
AhdI GACnn_n'nnGTC
Cuts at: 4205 4205
Size: 5476
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5476
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5476
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2210 2242 2210
Size: 32 5444
AlwNI CAG_nnn'CTG
Cuts at: 3219 4791 3219
Size: 1572 3904
ApaI G_GGCC'C
Cuts at: 2285 2285
Size: 5476
ApaLI G'TGCA_C
Cuts at: 2154 3533 4689 2154
Size: 1379 1156 2941
Fragments arranged by size:
2941 1379 1156
ApoI r'AATT_y
Cuts at: 1493 2785 3011 3163 5272 1493
Size: 1292 226 152 2109 1697
Fragments arranged by size:
2109 1697 1292 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4030 160
Size: 872 2998 1606
Fragments arranged by size:
2998 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5476
AvaI C'yCGr_G
Cuts at: 1870 1943 2801 1870
Size: 73 858 4545
Fragments arranged by size:
4545 858 73
BamHI G'GATC_C
Cuts at: 2806 3173 2806
Size: 367 5109
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2344 2571 2622 2797 4252
Size: 325 217 627 557 227 51 175 1455
Cuts at: 4252 618
Size: 1842
Fragments arranged by size:
1842 1455 627 557 325 227 217 175
51
BanII G_rGCy'C
Cuts at: 1391 2084 2285 2382 2795 1391
Size: 693 201 97 413 4072
Fragments arranged by size:
4072 693 413 201 97
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4505
BbvCI CC'TCA_GC
Cuts at: 2334 2334
Size: 5476
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2677 2711 3667 3701 1903
Size: 34 740 34 956 34 3678
Fragments arranged by size:
3678 956 740 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3368 4578 3368
Size: 1210 4266
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5476
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4087 136
Size: 107 122 71 631 605 2415 1525
Fragments arranged by size:
2415 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5471 5471
Size: 5476
BlpI GC'TnA_GC
Cuts at: 2486 2893 2486
Size: 407 5069
Bme1580I G_kGCm'C
Cuts at: 1790 2158 2285 2349 2574 2627 3537 4693 1790
Size: 368 127 64 225 53 910 1156 2573
Fragments arranged by size:
2573 1156 910 368 225 127 64 53
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5476
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2602 3207 4165
Size: 416 53 83 399 24 1185 605 958
Cuts at: 4165 442
Size: 1753
Fragments arranged by size:
1753 1185 958 605 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5476
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2068 2393 2483 2494 2501 4136 1239
Size: 829 325 90 11 7 1635 2579
Fragments arranged by size:
2579 1635 829 325 90 11 7
Bpu10I CC'TnA_GC
Cuts at: 2334 2771 2334
Size: 437 5039
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2075 2342 2660 3601 4466 4764 2075
Size: 267 318 941 865 298 2787
Fragments arranged by size:
2787 941 865 318 298 267
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4139 882
Size: 617 2640 2219
Fragments arranged by size:
2640 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3267 493
Size: 711 2063 2702
Fragments arranged by size:
2702 2063 711
BsaBI GATnn'nnATC
Cuts at: 5470 5470
Size: 5476
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3283 3665 4300 275
Size: 53 83 186 936 1750 382 635 1451
Fragments arranged by size:
1750 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3909 4307 4581 4728 5139 3909
Size: 398 274 147 411 4246
Fragments arranged by size:
4246 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5251
Fragments arranged by size:
5251 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2390 2648 1186
Size: 1204 258 4014
Fragments arranged by size:
4014 1204 258
BseYI C'CCAG_C
Cuts at: 2277 2348 2381 2421 2626 4679 5175 2277
Size: 71 33 40 205 2053 496 2578
Fragments arranged by size:
2578 2053 496 205 71 40 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2462 1163
Size: 573 726 4177
Fragments arranged by size:
4177 726 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2926 3687 4715 1540
Size: 275 186 925 761 1028 2301
Fragments arranged by size:
2301 1028 925 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2012 2084 2158 3537 3622 4693 2012
Size: 72 74 1379 85 1071 2795
Fragments arranged by size:
2795 1379 1071 85 74 72
BsmI GAATG_Cn'
Cuts at: 2987 3080 2987
Size: 93 5383
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2084 2158 2285 2349 2382 2574
Size: 399 222 72 74 127 64 33 192
Cuts at: 2574 2627 2795 3537 3622 4693 1391
Size: 53 168 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 222 192 168 127
85 74 72 64 53 33
BspEI T'CCGG_A
Cuts at: 5139 5139
Size: 5476
BspHI T'CATG_A
Cuts at: 3365 5095 3365
Size: 1730 3746
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2177 2229 2583 2831 844
Size: 1333 52 354 248 3489
Fragments arranged by size:
3489 1333 354 248 52
BsrBI CCG'CTC
Cuts at: 1394 1823 3363 1394
Size: 429 1540 3507
Fragments arranged by size:
3507 1540 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3971 4145 65
Size: 1183 2723 174 1396
Fragments arranged by size:
2723 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2136 4120 4307 1700
Size: 110 326 1984 187 2869
Fragments arranged by size:
2869 1984 326 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5476
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5476
BssSI C'ACGA_G
Cuts at: 3536 4548 3536
Size: 1012 4464
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5476
BstBI TT'CG_AA
Cuts at: 4280 4280
Size: 5476
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5476
BstXI CCAn_nnnn'nTGG
Cuts at: 2203 2232 2203
Size: 29 5447
BstYI r'GATC_y
Cuts at: 1972 2806 3173 3558 3575 5027 5113 5125 5471
Size: 834 367 385 17 1452 86 12 346
Cuts at: 5471 1972
Size: 1977
Fragments arranged by size:
1977 1452 834 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2213 2213
Size: 5476
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4924
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2147 2725 487
Size: 683 56 921 578 3238
Fragments arranged by size:
3238 921 683 578 56
BtsI GCAGTG_nn'
Cuts at: 2395 2404 2450 2992 3786 3806 2395
Size: 9 46 542 794 20 4065
Fragments arranged by size:
4065 794 542 46 20 9
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5476
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5441
DraI TTT'AAA
Cuts at: 2780 2780
Size: 5476
DraIII CAC_nnn'GTG
Cuts at: 2596 2596
Size: 5476
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4483 809
Size: 507 3167 1802
Fragments arranged by size:
3167 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2193 2401 2923
Size: 54 1425 50 275 18 363 208 522
Cuts at: 2923 3812 5441 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 522 363 275 208 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2923 1537
Size: 275 1111 4090
Fragments arranged by size:
4090 1111 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2294 2531 2714 3406 1353
Size: 941 237 183 692 3423
Fragments arranged by size:
3423 941 692 237 183
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4063 4437 4583 387
Size: 1064 138 2474 374 146 1280
Fragments arranged by size:
2474 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2082 2082
Size: 5476
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2068 2393 2483 2494 2501 3539 4136
Size: 393 436 325 90 11 7 1038 597
Cuts at: 4136 4923 5191 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1038 787 597 436 393 325 268
90 11 7
EcoNI CCTnn'n_nnAGG
Cuts at: 2212 2579 2212
Size: 367 5109
EcoO109I rG'GnC_Cy
Cuts at: 2025 2281 2282 2338 2704 2025
Size: 256 1 56 366 4797
Fragments arranged by size:
4797 366 256 56 1
EcoRI G'AATT_C
Cuts at: 2785 3163 2785
Size: 378 5098
FspI TGC'GCA
Cuts at: 3982 3982
Size: 5476
HaeII r_GCGC'y
Cuts at: 1678 4623 1678
Size: 2945 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4117 4149 4191 4223 1373
Size: 32 135 32 2545 32 42 32 2626
Fragments arranged by size:
2626 2545 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2737 2820 3066 669
Size: 988 281 799 83 246 3079
Fragments arranged by size:
3079 988 799 281 246 83
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5476
HpaI GTT'AAC
Cuts at: 3066 3066
Size: 5476
KpnI G_GTAC'C
Cuts at: 2801 2801
Size: 5476
MfeI C'AATT_G
Cuts at: 3075 3075
Size: 5476
MluI A'CGCG_T
Cuts at: 5352 5352
Size: 5476
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2711 4590 4774 1305
Size: 1406 1879 184 2007
Fragments arranged by size:
2007 1879 1406 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2195 2403 10
Size: 54 1425 343 363 208 3083
Fragments arranged by size:
3083 1425 363 343 208 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3434 3793 3952 518
Size: 648 2268 359 159 2042
Fragments arranged by size:
2268 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2118 2239 2257 2892 3569 4717 4962 1606
Size: 512 121 18 635 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 635 512 245 121 18
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4924
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5476
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5476
NotI GC'GGCC_GC
Cuts at: 2923 2923
Size: 5476
NspI r_CATG'y
Cuts at: 1132 1229 2764 2834 4379 1132
Size: 97 1535 70 1545 2229
Fragments arranged by size:
2229 1545 1535 97 70
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5476
PciI A'CATG_T
Cuts at: 4375 4375
Size: 5476
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5476
PfoI T'CCnGG_A
Cuts at: 2610 2610
Size: 5476
PmeI GTTT'AAAC
Cuts at: 2780 2780
Size: 5476
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3545 3577 5058 5090 3545
Size: 32 1481 32 3931
Fragments arranged by size:
3931 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2704 2704
Size: 5476
PshAI GACnn'nnGTC
Cuts at: 1613 2651 1613
Size: 1038 4438
PsiI TTA'TAA
Cuts at: 3046 3046
Size: 5476
PspOMI G'GGCC_C
Cuts at: 2281 2281
Size: 5476
PstI C_TGCA'G
Cuts at: 830 2271 2580 2828 830
Size: 1441 309 248 3478
Fragments arranged by size:
3478 1441 309 248
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5476
PvuII CAG'CTG
Cuts at: 2239 2239
Size: 5476
SacI G_AGCT'C
Cuts at: 2084 2084
Size: 5476
SalI G'TCGA_C
Cuts at: 1936 2818 1936
Size: 882 4594
SbfI CC_TGCA'GG
Cuts at: 2828 2828
Size: 5476
ScaI AGT'ACT
Cuts at: 3724 5436 3724
Size: 1712 3764
SfcI C'TryA_G
Cuts at: 826 1045 2267 2576 2824 3959 4640 4831 826
Size: 219 1222 309 248 1135 681 191 1471
Fragments arranged by size:
1471 1222 1135 681 309 248 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5476
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5476
SmaI CCC'GGG
Cuts at: 1872 2803 1872
Size: 931 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2090 2357 2675 3580 4481 4743
Size: 197 926 147 267 318 905 901 262
Cuts at: 4743 820
Size: 1553
Fragments arranged by size:
1553 926 905 901 318 267 262 197
147
SnaBI TAC'GTA
Cuts at: 493 3267 493
Size: 2774 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5476
SphI G_CATG'C
Cuts at: 1132 2834 1132
Size: 1702 3774
SspI AAT'ATT
Cuts at: 5 52 3400 5
Size: 47 3348 2081
Fragments arranged by size:
3348 2081 47
StuI AGG'CCT
Cuts at: 1517 2180 2547 1517
Size: 663 367 4446
Fragments arranged by size:
4446 663 367
StyI C'CwwG_G
Cuts at: 513 1065 2181 2493 2635 2915 513
Size: 552 1116 312 142 280 3074
Fragments arranged by size:
3074 1116 552 312 280 142
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2584 3503 3520 3673 3858 4303 325
Size: 1255 1004 919 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1004 919 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3722 5434 96
Size: 274 80 33 51 1443 1745 1712 138
Fragments arranged by size:
1745 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2606 2638 5436 5468 2606
Size: 32 2798 32 2614
Fragments arranged by size:
2798 2614 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5476
XbaI T'CTAG_A
Cuts at: 2812 2812
Size: 5476
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5476
XmaI C'CCGG_G
Cuts at: 1870 2801 1870
Size: 931 4545
XmnI GAAnn'nnTTC
Cuts at: 3605 3605
Size: 5476
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3284 276
Size: 53 83 186 936 1750 2468
Fragments arranged by size:
2468 1750 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BsmBI BstZ17I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI