Restriction map for
pFN21AB8914
(Circular) (Six-base) MAPSORT of: pFN21AB8914.seq Check: 1045 from: 1 to: 5470
pFN21AB8914 5470 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3280 278
Size: 53 83 186 936 1744 2468
Fragments arranged by size:
2468 1744 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2403 2813 1937
Size: 466 410 4594
Fragments arranged by size:
4594 466 410
Acc65I G'GTAC_C
Cuts at: 2791 2791
Size: 5470
AclI AA'CG_TT
Cuts at: 1563 3597 3970 1563
Size: 2034 373 3063
Fragments arranged by size:
3063 2034 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3533 4917 5185 1632
Size: 1901 1384 268 1917
Fragments arranged by size:
1917 1901 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5273
AflIII A'CryG_T
Cuts at: 1203 4369 5346 1203
Size: 3166 977 1327
Fragments arranged by size:
3166 1327 977
AgeI A'CCGG_T
Cuts at: 2453 4301 2453
Size: 1848 3622
AhdI GACnn_n'nnGTC
Cuts at: 4199 4199
Size: 5470
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5470
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5470
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2036 2068 2036
Size: 32 5438
AlwNI CAG_nnn'CTG
Cuts at: 3213 4785 3213
Size: 1572 3898
ApaLI G'TGCA_C
Cuts at: 2423 3527 4683 2423
Size: 1104 1156 3210
Fragments arranged by size:
3210 1156 1104
ApoI r'AATT_y
Cuts at: 1493 2431 2519 2779 3005 3157 5266 1493
Size: 938 88 260 226 152 2109 1697
Fragments arranged by size:
2109 1697 938 260 226 152 88
AseI AT'TA_AT
Cuts at: 160 1032 4024 160
Size: 872 2992 1606
Fragments arranged by size:
2992 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5470
AvaI C'yCGr_G
Cuts at: 1870 1943 2149 2204 2334 2372 2795 1870
Size: 73 206 55 130 38 423 4545
Fragments arranged by size:
4545 423 206 130 73 55 38
AvrII C'CTAG_G
Cuts at: 2089 2089
Size: 5470
BamHI G'GATC_C
Cuts at: 2800 3167 2800
Size: 367 5103
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2102 2305 2317 2330 2547
Size: 325 217 627 315 203 12 13 217
Cuts at: 2547 2791 4246 618
Size: 244 1455 1842
Fragments arranged by size:
1842 1455 627 325 315 244 217 217
203 13 12
BanII G_rGCy'C
Cuts at: 1391 2157 2789 1391
Size: 766 632 4072
Fragments arranged by size:
4072 766 632
BbeI G_GCGC'C
Cuts at: 2106 2309 2321 2334 2551 2106
Size: 203 12 13 217 5025
Fragments arranged by size:
5025 217 203 13 12
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4499
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3661 3695 1903
Size: 34 1724 34 3678
Fragments arranged by size:
3678 1724 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3362 4572 3362
Size: 1210 4260
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5470
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2152 4081 136
Size: 107 122 71 631 605 480 1929 1525
Fragments arranged by size:
1929 1525 631 605 480 122 107 71
BglII A'GATC_T
Cuts at: 2601 5465 2601
Size: 2864 2606
BlpI GC'TnA_GC
Cuts at: 2887 2887
Size: 5470
Bme1580I G_kGCm'C
Cuts at: 1790 2427 3531 4687 1790
Size: 637 1104 1156 2573
Fragments arranged by size:
2573 1156 1104 637
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5470
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3201 4159 442
Size: 416 53 83 399 24 1784 958 1753
Fragments arranged by size:
1784 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5470
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2262 4130 1239
Size: 1023 1868 2579
Fragments arranged by size:
2579 1868 1023
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2369 3595 4460 4758 2369
Size: 1226 865 298 3081
Fragments arranged by size:
3081 1226 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4133 882
Size: 617 2634 2219
Fragments arranged by size:
2634 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2428 3261 493
Size: 711 1224 833 2702
Fragments arranged by size:
2702 1224 833 711
BsaBI GATnn'nnATC
Cuts at: 2561 2600 5464 2561
Size: 39 2864 2567
Fragments arranged by size:
2864 2567 39
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2103 2306 2318 2331
Size: 53 83 186 936 570 203 12 13
Cuts at: 2331 2548 3277 3659 4294 275
Size: 217 729 382 635 1451
Fragments arranged by size:
1451 936 729 635 570 382 217 203
186 83 53 13 12
BsaWI w'CCGG_w
Cuts at: 2453 2616 3903 4301 4575 4722 5133 2453
Size: 163 1287 398 274 147 411 2790
Fragments arranged by size:
2790 1287 411 398 274 163 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5245
Fragments arranged by size:
5245 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2081 2677 2689 2692 1186
Size: 895 596 12 3 3964
Fragments arranged by size:
3964 895 596 12 3
BseYI C'CCAG_C
Cuts at: 2293 2714 4673 5169 2293
Size: 421 1959 496 2594
Fragments arranged by size:
2594 1959 496 421
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4897
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2397 2920 3681 4709 1540
Size: 275 186 396 523 761 1028 2301
Fragments arranged by size:
2301 1028 761 523 396 275 186
BsiHKAI G_wGCw'C
Cuts at: 2427 3531 3616 4687 2427
Size: 1104 85 1071 3210
Fragments arranged by size:
3210 1104 1071 85
BsmI GAATG_Cn'
Cuts at: 2981 3074 2981
Size: 93 5377
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2157 2427 2789 3531 3616 4687 1391
Size: 399 367 270 362 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 367 362 270 85
BspEI T'CCGG_A
Cuts at: 5133 5133
Size: 5470
BspHI T'CATG_A
Cuts at: 2010 3359 5089 2010
Size: 1349 1730 2391
Fragments arranged by size:
2391 1730 1349
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2655 2825 844
Size: 1811 170 3489
Fragments arranged by size:
3489 1811 170
BsrBI CCG'CTC
Cuts at: 1394 1823 2276 3357 1394
Size: 429 453 1081 3507
Fragments arranged by size:
3507 1081 453 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3965 4139 65
Size: 1183 2717 174 1396
Fragments arranged by size:
2717 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2314 2392 2453 4114 4301 1700
Size: 110 504 78 61 1661 187 2869
Fragments arranged by size:
2869 1661 504 187 110 78 61
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5470
BssHII G'CGCG_C
Cuts at: 1925 2124 1925
Size: 199 5271
BssSI C'ACGA_G
Cuts at: 3530 4542 3530
Size: 1012 4458
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5470
BstBI TT'CG_AA
Cuts at: 4274 4274
Size: 5470
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5470
BstXI CCAn_nnnn'nTGG
Cuts at: 2041 2041
Size: 5470
BstYI r'GATC_y
Cuts at: 1972 2601 2800 3167 3552 3569 5021 5107 5119
Size: 629 199 367 385 17 1452 86 12
Cuts at: 5119 5465 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 629 385 367 346 199 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2162 2397 513
Size: 552 939 158 235 3586
Fragments arranged by size:
3586 939 552 235 158
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4731
Fragments arranged by size:
4731 683 56
BtsI GCAGTG_nn'
Cuts at: 2986 3780 3800 2986
Size: 794 20 4656
Fragments arranged by size:
4656 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5470
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5435
DraI TTT'AAA
Cuts at: 2774 2774
Size: 5470
DraIII CAC_nnn'GTG
Cuts at: 2577 2577
Size: 5470
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4477 809
Size: 507 3161 1802
Fragments arranged by size:
3161 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2394 2736 2917
Size: 54 1425 50 275 18 564 342 181
Cuts at: 2917 3806 5435 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 564 342 275 181 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2394 2917 1537
Size: 275 582 523 4090
Fragments arranged by size:
4090 582 523 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3400 1353
Size: 2047 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2301 4057 4431 4577 387
Size: 1064 138 712 1756 374 146 1280
Fragments arranged by size:
1756 1280 1064 712 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2262 3533 4130 4917 5185 1239
Size: 393 630 1271 597 787 268 1524
Fragments arranged by size:
1524 1271 787 630 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2190 2190
Size: 5470
EcoRI G'AATT_C
Cuts at: 2431 2779 3157 2431
Size: 348 378 4744
Fragments arranged by size:
4744 378 348
FspI TGC'GCA
Cuts at: 3976 3976
Size: 5470
HaeII r_GCGC'y
Cuts at: 1678 2106 2222 2309 2321 2334 2551 2593 4617
Size: 428 116 87 12 13 217 42 2024
Cuts at: 4617 1678
Size: 2531
Fragments arranged by size:
2531 2024 428 217 116 87 42 13
12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4111 4143 4185 4217 1373
Size: 32 135 32 2539 32 42 32 2626
Fragments arranged by size:
2626 2539 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2814 3060 669
Size: 988 281 876 246 3079
Fragments arranged by size:
3079 988 876 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5470
HpaI GTT'AAC
Cuts at: 3060 3060
Size: 5470
KasI G'GCGC_C
Cuts at: 2102 2305 2317 2330 2547 2102
Size: 203 12 13 217 5025
Fragments arranged by size:
5025 217 203 13 12
KpnI G_GTAC'C
Cuts at: 2795 2795
Size: 5470
MfeI C'AATT_G
Cuts at: 3069 3069
Size: 5470
MluI A'CGCG_T
Cuts at: 5346 5346
Size: 5470
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2376 2534 2565 4584 4768 1305
Size: 1071 158 31 2019 184 2007
Fragments arranged by size:
2019 2007 1071 184 158 31
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2738 10
Size: 54 1425 343 906 2742
Fragments arranged by size:
2742 1425 906 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3428 3787 3946 518
Size: 648 843 1419 359 159 2042
Fragments arranged by size:
2042 1419 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2199 2886 3563 4711 4956 1606
Size: 593 687 677 1148 245 2120
Fragments arranged by size:
2120 1148 687 677 593 245
NaeI GCC'GGC
Cuts at: 2316 2394 2316
Size: 78 5392
NarI GG'CG_CC
Cuts at: 2103 2306 2318 2331 2548 2103
Size: 203 12 13 217 5025
Fragments arranged by size:
5025 217 203 13 12
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3979
Fragments arranged by size:
3979 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5470
NgoMIV G'CCGG_C
Cuts at: 2314 2392 2314
Size: 78 5392
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5470
NotI GC'GGCC_GC
Cuts at: 2917 2917
Size: 5470
NspI r_CATG'y
Cuts at: 1132 1229 2828 4373 1132
Size: 97 1599 1545 2229
Fragments arranged by size:
2229 1599 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5470
PciI A'CATG_T
Cuts at: 4369 4369
Size: 5470
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5470
PfoI T'CCnGG_A
Cuts at: 2278 2278
Size: 5470
PmeI GTTT'AAAC
Cuts at: 2774 2774
Size: 5470
PmlI CAC'GTG
Cuts at: 2428 2428
Size: 5470
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3539 3571 5052 5084 3539
Size: 32 1481 32 3925
Fragments arranged by size:
3925 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5470
PsiI TTA'TAA
Cuts at: 3040 3040
Size: 5470
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2432 2464 2432
Size: 32 5438
PstI C_TGCA'G
Cuts at: 830 2822 830
Size: 1992 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5470
SalI G'TCGA_C
Cuts at: 1936 2812 1936
Size: 876 4594
SbfI CC_TGCA'GG
Cuts at: 2822 2822
Size: 5470
ScaI AGT'ACT
Cuts at: 3718 5430 3718
Size: 1712 3758
SfcI C'TryA_G
Cuts at: 826 1045 2341 2818 3953 4634 4825 826
Size: 219 1296 477 1135 681 191 1471
Fragments arranged by size:
1471 1296 1135 681 477 219 191
SfoI GGC'GCC
Cuts at: 2104 2307 2319 2332 2549 2104
Size: 203 12 13 217 5025
Fragments arranged by size:
5025 217 203 13 12
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5470
SgrAI Cr'CCGG_yG
Cuts at: 1700 2314 2453 1700
Size: 614 139 4717
Fragments arranged by size:
4717 614 139
SmaI CCC'GGG
Cuts at: 1872 2151 2206 2336 2374 2797 1872
Size: 279 55 130 38 423 4545
Fragments arranged by size:
4545 423 279 130 55 38
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2384 3574 4475 4737 820
Size: 197 926 441 1190 901 262 1553
Fragments arranged by size:
1553 1190 926 901 441 262 197
SnaBI TAC'GTA
Cuts at: 493 3261 493
Size: 2768 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5470
SphI G_CATG'C
Cuts at: 1132 2828 1132
Size: 1696 3774
SrfI GCCC'GGGC
Cuts at: 2336 2336
Size: 5470
SspI AAT'ATT
Cuts at: 5 52 3394 5
Size: 47 3342 2081
Fragments arranged by size:
3342 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5470
StyI C'CwwG_G
Cuts at: 513 1065 2004 2089 2909 513
Size: 552 939 85 820 3074
Fragments arranged by size:
3074 939 820 552 85
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3497 3514 3667 3852 4297 325
Size: 1255 1917 17 153 185 445 1498
Fragments arranged by size:
1917 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3716 5428 96
Size: 274 80 33 51 1443 1739 1712 138
Fragments arranged by size:
1739 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5430 5462 5430
Size: 32 5438
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5470
XbaI T'CTAG_A
Cuts at: 2806 2806
Size: 5470
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5470
XmaI C'CCGG_G
Cuts at: 1870 2149 2204 2334 2372 2795 1870
Size: 279 55 130 38 423 4545
Fragments arranged by size:
4545 423 279 130 55 38
XmnI GAAnn'nnTTC
Cuts at: 3599 3599
Size: 5470
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3278 276
Size: 53 83 186 936 1744 2468
Fragments arranged by size:
2468 1744 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PsrI PstI PvuI
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoO109I EcoRV
FalI FseI FspAI NruI NsiI PacI PpuMI PspOMI
PspXI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SwaI XcmI