Restriction map for
pFN21AB8913
(Circular) (Six-base) MAPSORT of: pFN21AB8913.seq Check: 4319 from: 1 to: 5464
pFN21AB8913 5464 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3274 278
Size: 53 83 186 936 1738 2468
Fragments arranged by size:
2468 1738 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2598 2807 1937
Size: 661 209 4594
Fragments arranged by size:
4594 661 209
Acc65I G'GTAC_C
Cuts at: 2785 2785
Size: 5464
AclI AA'CG_TT
Cuts at: 1563 3591 3964 1563
Size: 2028 373 3063
Fragments arranged by size:
3063 2028 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2690 3527 4911 5179 1632
Size: 1058 837 1384 268 1917
Fragments arranged by size:
1917 1384 1058 837 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5267
AflIII A'CryG_T
Cuts at: 1203 4363 5340 1203
Size: 3160 977 1327
Fragments arranged by size:
3160 1327 977
AgeI A'CCGG_T
Cuts at: 4295 4295
Size: 5464
AhdI GACnn_n'nnGTC
Cuts at: 4193 4193
Size: 5464
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5464
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5464
AlwNI CAG_nnn'CTG
Cuts at: 2366 3207 4779 2366
Size: 841 1572 3051
Fragments arranged by size:
3051 1572 841
ApaLI G'TGCA_C
Cuts at: 3521 4677 3521
Size: 1156 4308
ApoI r'AATT_y
Cuts at: 1493 2773 2999 3151 5260 1493
Size: 1280 226 152 2109 1697
Fragments arranged by size:
2109 1697 1280 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4018 160
Size: 872 2986 1606
Fragments arranged by size:
2986 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5464
AvaI C'yCGr_G
Cuts at: 1870 1943 2079 2259 2789 1870
Size: 73 136 180 530 4545
Fragments arranged by size:
4545 530 180 136 73
BamHI G'GATC_C
Cuts at: 2794 3161 2794
Size: 367 5097
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2129 2208 2785 4240 618
Size: 325 217 627 342 79 577 1455 1842
Fragments arranged by size:
1842 1455 627 577 342 325 217 79
BanII G_rGCy'C
Cuts at: 1391 2049 2783 1391
Size: 658 734 4072
Fragments arranged by size:
4072 734 658
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2478 928
Size: 971 579 3914
Fragments arranged by size:
3914 971 579
BbvCI CC'TCA_GC
Cuts at: 2061 2061
Size: 5464
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3655 3689 1903
Size: 34 1718 34 3678
Fragments arranged by size:
3678 1718 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3356 4566 3356
Size: 1210 4254
BclI T'GATC_A
Cuts at: 1548 2178 1548
Size: 630 4834
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2057 4075
Size: 107 122 71 631 605 337 48 2018
Cuts at: 4075 136
Size: 1525
Fragments arranged by size:
2018 1525 631 605 337 122 107 71
48
BglII A'GATC_T
Cuts at: 5459 5459
Size: 5464
BlpI GC'TnA_GC
Cuts at: 2881 2881
Size: 5464
Bme1580I G_kGCm'C
Cuts at: 1790 3525 4681 1790
Size: 1735 1156 2573
Fragments arranged by size:
2573 1735 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5464
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2294 3195 4153
Size: 416 53 83 399 24 877 901 958
Cuts at: 4153 442
Size: 1753
Fragments arranged by size:
1753 958 901 877 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5464
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2618 2618
Size: 5464
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2132 2240 2633 4124 1239
Size: 893 108 393 1491 2579
Fragments arranged by size:
2579 1491 893 393 108
Bpu10I CC'TnA_GC
Cuts at: 2061 2061
Size: 5464
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2122 3589 4454 4752 2122
Size: 1467 865 298 2834
Fragments arranged by size:
2834 1467 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2423 4127 882
Size: 617 924 1704 2219
Fragments arranged by size:
2219 1704 924 617
BsaAI yAC'GTr
Cuts at: 493 1204 3255 493
Size: 711 2051 2702
Fragments arranged by size:
2702 2051 711
BsaBI GATnn'nnATC
Cuts at: 5458 5458
Size: 5464
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3271 3653 4288 275
Size: 53 83 186 936 1738 382 635 1451
Fragments arranged by size:
1738 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3897 4295 4569 4716 5127 3897
Size: 398 274 147 411 4234
Fragments arranged by size:
4234 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2669 2699 1178
Size: 30 165 30 1266 30 3943
Fragments arranged by size:
3943 1266 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2057 2258 2677 1186
Size: 871 201 419 3973
Fragments arranged by size:
3973 871 419 201
BseYI C'CCAG_C
Cuts at: 4667 5163 4667
Size: 496 4968
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2270 1163
Size: 573 534 4357
Fragments arranged by size:
4357 573 534
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2034 2914 3675 4703 1540
Size: 275 186 33 880 761 1028 2301
Fragments arranged by size:
2301 1028 880 761 275 186 33
BsiHKAI G_wGCw'C
Cuts at: 2547 3525 3610 4681 2547
Size: 978 85 1071 3330
Fragments arranged by size:
3330 1071 978 85
BsmI GAATG_Cn'
Cuts at: 2466 2975 3068 2466
Size: 509 93 4862
Fragments arranged by size:
4862 509 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2049 2547 2783 3525 3610 4681 1391
Size: 399 259 498 236 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 498 399 259 236 85
BspEI T'CCGG_A
Cuts at: 5127 5127
Size: 5464
BspHI T'CATG_A
Cuts at: 3353 5083 3353
Size: 1730 3734
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2437 2819 844
Size: 1593 382 3489
Fragments arranged by size:
3489 1593 382
BsrBI CCG'CTC
Cuts at: 1394 1823 2086 2106 3351 1394
Size: 429 263 20 1245 3507
Fragments arranged by size:
3507 1245 429 263 20
BsrDI GCAATG_nn'
Cuts at: 65 1248 3959 4133 65
Size: 1183 2711 174 1396
Fragments arranged by size:
2711 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2029 2205 4108 4295 1700
Size: 110 219 176 1903 187 2869
Fragments arranged by size:
2869 1903 219 187 176 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5464
BssHII G'CGCG_C
Cuts at: 1925 2138 1925
Size: 213 5251
BssSI C'ACGA_G
Cuts at: 3524 4536 3524
Size: 1012 4452
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5464
BstBI TT'CG_AA
Cuts at: 4268 4268
Size: 5464
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5464
BstYI r'GATC_y
Cuts at: 1972 2794 3161 3546 3563 5015 5101 5113 5459
Size: 822 367 385 17 1452 86 12 346
Cuts at: 5459 1972
Size: 1977
Fragments arranged by size:
1977 1452 822 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2149 2754 513
Size: 552 939 145 605 3223
Fragments arranged by size:
3223 939 605 552 145
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4725
Fragments arranged by size:
4725 683 56
BtsI GCAGTG_nn'
Cuts at: 2980 3774 3794 2980
Size: 794 20 4650
Fragments arranged by size:
4650 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5464
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5429
DraI TTT'AAA
Cuts at: 2768 2768
Size: 5464
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2606 4471 809
Size: 507 1290 1865 1802
Fragments arranged by size:
1865 1802 1290 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2013 2031 2070
Size: 54 1425 50 275 18 183 18 39
Cuts at: 2070 2152 2911 3800 5429 8
Size: 82 759 889 1629 43
Fragments arranged by size:
1629 1425 889 759 275 183 82 54
50 43 39 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 2031 2911 1537
Size: 275 219 880 4090
Fragments arranged by size:
4090 880 275 219
EarI CTCTTCn'nnn_
Cuts at: 1353 2666 3394 1353
Size: 1313 728 3423
Fragments arranged by size:
3423 1313 728
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2257 4051 4425 4571 387
Size: 1064 138 668 1794 374 146 1280
Fragments arranged by size:
1794 1280 1064 668 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2132 2240 2633 2690 3527 4124 4911
Size: 393 500 108 393 57 837 597 787
Cuts at: 4911 5179 1239
Size: 268 1524
Fragments arranged by size:
1524 837 787 597 500 393 393 268
108 57
EcoNI CCTnn'n_nnAGG
Cuts at: 2615 2615
Size: 5464
EcoRI G'AATT_C
Cuts at: 2773 3151 2773
Size: 378 5086
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2206 2206
Size: 5464
FspI TGC'GCA
Cuts at: 3970 3970
Size: 5464
HaeII r_GCGC'y
Cuts at: 1678 2219 2267 4611 1678
Size: 541 48 2344 2531
Fragments arranged by size:
2531 2344 541 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4105 4137 4179 4211 1373
Size: 32 135 32 2533 32 42 32 2626
Fragments arranged by size:
2626 2533 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2808 3054 669
Size: 988 281 870 246 3079
Fragments arranged by size:
3079 988 870 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5464
HpaI GTT'AAC
Cuts at: 3054 3054
Size: 5464
KpnI G_GTAC'C
Cuts at: 2789 2789
Size: 5464
MfeI C'AATT_G
Cuts at: 2439 3063 2439
Size: 624 4840
MluI A'CGCG_T
Cuts at: 5340 5340
Size: 5464
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2352 2448 2526 4578 4762 1305
Size: 1047 96 78 2052 184 2007
Fragments arranged by size:
2052 2007 1047 184 96 78
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3642
Fragments arranged by size:
3642 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3422 3781 3940 518
Size: 648 2256 359 159 2042
Fragments arranged by size:
2256 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2274 2533 2880 3557 4705 4950 1606
Size: 668 259 347 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 668 347 259 245
NaeI GCC'GGC
Cuts at: 2031 2031
Size: 5464
NcoI C'CATG_G
Cuts at: 513 1065 2004 2754 513
Size: 552 939 750 3223
Fragments arranged by size:
3223 939 750 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5464
NgoMIV G'CCGG_C
Cuts at: 2029 2029
Size: 5464
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5464
NotI GC'GGCC_GC
Cuts at: 2911 2911
Size: 5464
NspI r_CATG'y
Cuts at: 1132 1229 2509 2822 4367 1132
Size: 97 1280 313 1545 2229
Fragments arranged by size:
2229 1545 1280 313 97
PasI CC'CwG_GG
Cuts at: 1348 2413 1348
Size: 1065 4399
PciI A'CATG_T
Cuts at: 4363 4363
Size: 5464
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5464
PfoI T'CCnGG_A
Cuts at: 2610 2610
Size: 5464
PmeI GTTT'AAAC
Cuts at: 2768 2768
Size: 5464
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3533 3565 5046 5078 3533
Size: 32 1481 32 3919
Fragments arranged by size:
3919 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5464
PsiI TTA'TAA
Cuts at: 3034 3034
Size: 5464
PstI C_TGCA'G
Cuts at: 830 2816 830
Size: 1986 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5464
PvuII CAG'CTG
Cuts at: 2533 2533
Size: 5464
SalI G'TCGA_C
Cuts at: 1936 2806 1936
Size: 870 4594
SapI GCTCTTCn'nnn_
Cuts at: 2666 2666
Size: 5464
SbfI CC_TGCA'GG
Cuts at: 2816 2816
Size: 5464
ScaI AGT'ACT
Cuts at: 3712 5424 3712
Size: 1712 3752
SfcI C'TryA_G
Cuts at: 826 1045 2812 3947 4628 4819 826
Size: 219 1767 1135 681 191 1471
Fragments arranged by size:
1767 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5464
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5464
SmaI CCC'GGG
Cuts at: 1872 2081 2261 2791 1872
Size: 209 180 530 4545
Fragments arranged by size:
4545 530 209 180
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2101 3568 4469 4731 820
Size: 197 926 158 1467 901 262 1553
Fragments arranged by size:
1553 1467 926 901 262 197 158
SnaBI TAC'GTA
Cuts at: 493 3255 493
Size: 2762 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5464
SphI G_CATG'C
Cuts at: 1132 2822 1132
Size: 1690 3774
SrfI GCCC'GGGC
Cuts at: 2261 2261
Size: 5464
SspI AAT'ATT
Cuts at: 5 52 3388 5
Size: 47 3336 2081
Fragments arranged by size:
3336 2081 47
StuI AGG'CCT
Cuts at: 1517 2621 1517
Size: 1104 4360
StyI C'CwwG_G
Cuts at: 513 1065 2004 2196 2754 2903 513
Size: 552 939 192 558 149 3074
Fragments arranged by size:
3074 939 558 552 192 149
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3491 3508 3661 3846 4291 325
Size: 1255 1911 17 153 185 445 1498
Fragments arranged by size:
1911 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3710 5422 96
Size: 274 80 33 51 1443 1733 1712 138
Fragments arranged by size:
1733 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5424 5456 5424
Size: 32 5432
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5464
XbaI T'CTAG_A
Cuts at: 2800 2800
Size: 5464
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5464
XmaI C'CCGG_G
Cuts at: 1870 2079 2259 2789 1870
Size: 209 180 530 4545
Fragments arranged by size:
4545 530 209 180
XmnI GAAnn'nnTTC
Cuts at: 2621 2664 3593 2621
Size: 43 929 4492
Fragments arranged by size:
4492 929 43
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3272 276
Size: 53 83 186 936 1738 2468
Fragments arranged by size:
2468 1738 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoO109I EcoRV FseI FspAI KasI NarI NruI NsiI
PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SexAI SfiI SfoI SwaI XcmI