Restriction map for
pFN21AB8912
(Circular) (Six-base) MAPSORT of: pFN21AB8912.seq Check: 5717 from: 1 to: 5458
pFN21AB8912 5458 bp
With 182 enzymes: SgfI *
March 28, 2012 13:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3268 278
Size: 53 83 186 936 1732 2468
Fragments arranged by size:
2468 1732 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2043 2427 2801 1937
Size: 106 384 374 4594
Fragments arranged by size:
4594 384 374 106
Acc65I G'GTAC_C
Cuts at: 2779 2779
Size: 5458
AclI AA'CG_TT
Cuts at: 1563 3585 3958 1563
Size: 2022 373 3063
Fragments arranged by size:
3063 2022 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3521 4905 5173 1632
Size: 1889 1384 268 1917
Fragments arranged by size:
1917 1889 1384 268
AfeI AGC'GCT
Cuts at: 2195 2330 2495 2195
Size: 135 165 5158
Fragments arranged by size:
5158 165 135
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5261
AflIII A'CryG_T
Cuts at: 1203 4357 5334 1203
Size: 3154 977 1327
Fragments arranged by size:
3154 1327 977
AgeI A'CCGG_T
Cuts at: 4289 4289
Size: 5458
AhdI GACnn_n'nnGTC
Cuts at: 4187 4187
Size: 5458
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5458
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5458
AlwNI CAG_nnn'CTG
Cuts at: 3201 4773 3201
Size: 1572 3886
ApaI G_GGCC'C
Cuts at: 2223 2308 2724 2223
Size: 85 416 4957
Fragments arranged by size:
4957 416 85
ApaLI G'TGCA_C
Cuts at: 2182 3515 4671 2182
Size: 1333 1156 2969
Fragments arranged by size:
2969 1333 1156
ApoI r'AATT_y
Cuts at: 1493 2543 2767 2993 3145 5254 1493
Size: 1050 224 226 152 2109 1697
Fragments arranged by size:
2109 1697 1050 226 224 152
AseI AT'TA_AT
Cuts at: 160 1032 4012 160
Size: 872 2980 1606
Fragments arranged by size:
2980 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5458
AvaI C'yCGr_G
Cuts at: 1870 1943 2108 2277 2692 2783 1870
Size: 73 165 169 415 91 4545
Fragments arranged by size:
4545 415 169 165 91 73
BamHI G'GATC_C
Cuts at: 2788 3155 2788
Size: 367 5091
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2571 2779 4234 618
Size: 325 217 627 784 208 1455 1842
Fragments arranged by size:
1842 1455 784 627 325 217 208
BanII G_rGCy'C
Cuts at: 1391 2223 2284 2308 2322 2421 2490 2724 2777
Size: 832 61 24 14 99 69 234 53
Cuts at: 2777 1391
Size: 4072
Fragments arranged by size:
4072 832 234 99 69 61 53 24
14
BbeI G_GCGC'C
Cuts at: 2575 2575
Size: 5458
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4487
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3649 3683 1903
Size: 34 1712 34 3678
Fragments arranged by size:
3678 1712 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3350 4560 3350
Size: 1210 4248
BclI T'GATC_A
Cuts at: 1548 2025 1548
Size: 477 4981
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4069 136
Size: 107 122 71 631 605 2397 1525
Fragments arranged by size:
2397 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2625 5453 2625
Size: 2828 2630
BlpI GC'TnA_GC
Cuts at: 2710 2875 2710
Size: 165 5293
Bme1580I G_kGCm'C
Cuts at: 1790 2186 2223 2308 2724 3519 4675 1790
Size: 396 37 85 416 795 1156 2573
Fragments arranged by size:
2573 1156 795 416 396 85 37
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5458
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3189 4147 442
Size: 416 53 83 399 24 1772 958 1753
Fragments arranged by size:
1772 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5458
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2555 4118 1239
Size: 1316 1563 2579
Fragments arranged by size:
2579 1563 1316
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3583 4448 4746 3583
Size: 865 298 4295
Fragments arranged by size:
4295 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4121 882
Size: 617 2622 2219
Fragments arranged by size:
2622 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2452 3249 493
Size: 711 1248 797 2702
Fragments arranged by size:
2702 1248 797 711
BsaBI GATnn'nnATC
Cuts at: 2624 5452 2624
Size: 2828 2630
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2572 3265 3647 4282
Size: 53 83 186 936 1039 693 382 635
Cuts at: 4282 275
Size: 1451
Fragments arranged by size:
1451 1039 936 693 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3891 4289 4563 4710 5121 3891
Size: 398 274 147 411 4228
Fragments arranged by size:
4228 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5233
Fragments arranged by size:
5233 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2300 1186
Size: 1114 4344
BseYI C'CCAG_C
Cuts at: 2093 2390 4661 5157 2093
Size: 297 2271 496 2394
Fragments arranged by size:
2394 2271 496 297
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2210 2369 1163
Size: 573 474 159 4252
Fragments arranged by size:
4252 573 474 159
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2908 3669 4697 1540
Size: 275 186 907 761 1028 2301
Fragments arranged by size:
2301 1028 907 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2186 2459 3519 3604 4675 2186
Size: 273 1060 85 1071 2969
Fragments arranged by size:
2969 1071 1060 273 85
BsmI GAATG_Cn'
Cuts at: 2969 3062 2969
Size: 93 5365
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2186 2223 2284 2308 2322 2421 2459
Size: 399 396 37 61 24 14 99 38
Cuts at: 2459 2490 2724 2777 3519 3604 4675 1391
Size: 31 234 53 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 396 234 99 85
61 53 38 37 31 24 14
BspEI T'CCGG_A
Cuts at: 5121 5121
Size: 5458
BspHI T'CATG_A
Cuts at: 3347 5077 3347
Size: 1730 3728
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2512 2813 844
Size: 1668 301 3489
Fragments arranged by size:
3489 1668 301
BsrBI CCG'CTC
Cuts at: 1394 1823 2691 3345 1394
Size: 429 868 654 3507
Fragments arranged by size:
3507 868 654 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3953 4127 65
Size: 1183 2705 174 1396
Fragments arranged by size:
2705 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2120 2477 2640 2730 4102 4289 1700
Size: 110 310 357 163 90 1372 187 2869
Fragments arranged by size:
2869 1372 357 310 187 163 110 90
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5458
BssHII G'CGCG_C
Cuts at: 1925 2173 2751 1925
Size: 248 578 4632
Fragments arranged by size:
4632 578 248
BssSI C'ACGA_G
Cuts at: 3518 4530 3518
Size: 1012 4446
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5458
BstBI TT'CG_AA
Cuts at: 4262 4262
Size: 5458
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5458
BstYI r'GATC_y
Cuts at: 1972 2625 2685 2788 3155 3540 3557 5009 5095
Size: 653 60 103 367 385 17 1452 86
Cuts at: 5095 5107 5453 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 653 385 367 346 103 86
60 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3967
Fragments arranged by size:
3967 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4719
Fragments arranged by size:
4719 683 56
BtsI GCAGTG_nn'
Cuts at: 2974 3768 3788 2974
Size: 794 20 4644
Fragments arranged by size:
4644 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5458
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5423
DraI TTT'AAA
Cuts at: 2762 2762
Size: 5458
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4465 809
Size: 507 3149 1802
Fragments arranged by size:
3149 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2125 2323 2728
Size: 54 1425 50 275 18 295 198 405
Cuts at: 2728 2905 3794 5423 8
Size: 177 889 1629 43
Fragments arranged by size:
1629 1425 889 405 295 275 198 177
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2905 1537
Size: 275 1093 4090
Fragments arranged by size:
4090 1093 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3388 1353
Size: 2035 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2223 4045 4419 4565 387
Size: 1064 138 634 1822 374 146 1280
Fragments arranged by size:
1822 1280 1064 634 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2555 3521 4118 4905 5173 1239
Size: 393 923 966 597 787 268 1524
Fragments arranged by size:
1524 966 923 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2212 2220 2304 2305 2720 2721 2744 2212
Size: 8 84 1 415 1 23 4926
Fragments arranged by size:
4926 415 84 23 8 1 1
EcoRI G'AATT_C
Cuts at: 2767 3145 2767
Size: 378 5080
FseI GG_CCGG'CC
Cuts at: 2734 2734
Size: 5458
FspI TGC'GCA
Cuts at: 2441 3964 2441
Size: 1523 3935
HaeII r_GCGC'y
Cuts at: 1678 2197 2332 2497 2575 2617 4605 1678
Size: 519 135 165 78 42 1988 2531
Fragments arranged by size:
2531 1988 519 165 135 78 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4099 4131 4173 4205 1373
Size: 32 135 32 2527 32 42 32 2626
Fragments arranged by size:
2626 2527 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2044 2802 3048 669
Size: 988 281 106 758 246 3079
Fragments arranged by size:
3079 988 758 281 246 106
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5458
HpaI GTT'AAC
Cuts at: 3048 3048
Size: 5458
KasI G'GCGC_C
Cuts at: 2571 2571
Size: 5458
KpnI G_GTAC'C
Cuts at: 2783 2783
Size: 5458
MfeI C'AATT_G
Cuts at: 3057 3057
Size: 5458
MluI A'CGCG_T
Cuts at: 5334 5334
Size: 5458
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4572 4756 1305
Size: 3267 184 2007
Fragments arranged by size:
3267 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3636
Fragments arranged by size:
3636 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3416 3775 3934 518
Size: 648 843 1407 359 159 2042
Fragments arranged by size:
2042 1407 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2874 3551 4699 4944 1606
Size: 1268 677 1148 245 2120
Fragments arranged by size:
2120 1268 1148 677 245
NaeI GCC'GGC
Cuts at: 2122 2479 2732 2122
Size: 357 253 4848
Fragments arranged by size:
4848 357 253
NarI GG'CG_CC
Cuts at: 2572 2572
Size: 5458
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3967
Fragments arranged by size:
3967 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5458
NgoMIV G'CCGG_C
Cuts at: 2120 2477 2730 2120
Size: 357 253 4848
Fragments arranged by size:
4848 357 253
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5458
NotI GC'GGCC_GC
Cuts at: 2905 2905
Size: 5458
NspI r_CATG'y
Cuts at: 1132 1229 2816 4361 1132
Size: 97 1587 1545 2229
Fragments arranged by size:
2229 1587 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2216 1348
Size: 868 4590
PciI A'CATG_T
Cuts at: 4357 4357
Size: 5458
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5458
PfoI T'CCnGG_A
Cuts at: 2313 2313
Size: 5458
PmeI GTTT'AAAC
Cuts at: 2762 2762
Size: 5458
PmlI CAC'GTG
Cuts at: 2452 2452
Size: 5458
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3527 3559 5040 5072 3527
Size: 32 1481 32 3913
Fragments arranged by size:
3913 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2212 2212
Size: 5458
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5458
PsiI TTA'TAA
Cuts at: 3028 3028
Size: 5458
PspOMI G'GGCC_C
Cuts at: 2219 2304 2720 2219
Size: 85 416 4957
Fragments arranged by size:
4957 416 85
PstI C_TGCA'G
Cuts at: 830 2810 830
Size: 1980 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5458
RsrII CG'GwC_CG
Cuts at: 2502 2502
Size: 5458
SalI G'TCGA_C
Cuts at: 1936 2042 2800 1936
Size: 106 758 4594
Fragments arranged by size:
4594 758 106
SanDI GG'GwC_CC
Cuts at: 2212 2212
Size: 5458
SbfI CC_TGCA'GG
Cuts at: 2810 2810
Size: 5458
ScaI AGT'ACT
Cuts at: 3706 5418 3706
Size: 1712 3746
SfcI C'TryA_G
Cuts at: 826 1045 2806 3941 4622 4813 826
Size: 219 1761 1135 681 191 1471
Fragments arranged by size:
1761 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2573 2573
Size: 5458
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5458
SgrAI Cr'CCGG_yG
Cuts at: 1700 2120 2477 1700
Size: 420 357 4681
Fragments arranged by size:
4681 420 357
SmaI CCC'GGG
Cuts at: 1872 2110 2785 1872
Size: 238 675 4545
Fragments arranged by size:
4545 675 238
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3562 4463 4725 820
Size: 197 926 1619 901 262 1553
Fragments arranged by size:
1619 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3249 493
Size: 2756 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5458
SphI G_CATG'C
Cuts at: 1132 2816 1132
Size: 1684 3774
SspI AAT'ATT
Cuts at: 5 52 2072 3382 5
Size: 47 2020 1310 2081
Fragments arranged by size:
2081 2020 1310 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5458
StyI C'CwwG_G
Cuts at: 513 1065 2004 2897 513
Size: 552 939 893 3074
Fragments arranged by size:
3074 939 893 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3485 3502 3655 3840 4285 325
Size: 1255 1905 17 153 185 445 1498
Fragments arranged by size:
1905 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3704 5416 96
Size: 274 80 33 51 1443 1727 1712 138
Fragments arranged by size:
1727 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5418 5450 5418
Size: 32 5426
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5458
XbaI T'CTAG_A
Cuts at: 2794 2794
Size: 5458
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5458
XmaI C'CCGG_G
Cuts at: 1870 2108 2783 1870
Size: 238 675 4545
Fragments arranged by size:
4545 675 238
XmnI GAAnn'nnTTC
Cuts at: 3587 3587
Size: 5458
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3266 276
Size: 53 83 186 936 1732 2468
Fragments arranged by size:
2468 1732 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
RsrII SalI SanDI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FspAI NruI NsiI PacI PspXI
PsrI PvuII SacI SacII SapI SexAI SfiI SrfI
SwaI XcmI