Restriction map for
pFN21AB8911
(Circular) (Six-base) MAPSORT of: pFN21AB8911.seq Check: 3571 from: 1 to: 5455
pFN21AB8911 5455 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3265 278
Size: 53 83 186 936 1729 2468
Fragments arranged by size:
2468 1729 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2040 2235 2798 1937
Size: 103 195 563 4594
Fragments arranged by size:
4594 563 195 103
Acc65I G'GTAC_C
Cuts at: 2283 2776 2283
Size: 493 4962
AclI AA'CG_TT
Cuts at: 1563 3582 3955 1563
Size: 2019 373 3063
Fragments arranged by size:
3063 2019 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3518 4902 5170 1632
Size: 1886 1384 268 1917
Fragments arranged by size:
1917 1886 1384 268
AfeI AGC'GCT
Cuts at: 2570 2570
Size: 5455
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5258
AflIII A'CryG_T
Cuts at: 1203 2211 4354 5331 1203
Size: 1008 2143 977 1327
Fragments arranged by size:
2143 1327 1008 977
AgeI A'CCGG_T
Cuts at: 4286 4286
Size: 5455
AhdI GACnn_n'nnGTC
Cuts at: 4184 4184
Size: 5455
AleI CACnn'nnGTG
Cuts at: 1166 2061 1166
Size: 895 4560
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5455
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2603 2635 2603
Size: 32 5423
AlwNI CAG_nnn'CTG
Cuts at: 2596 3198 4770 2596
Size: 602 1572 3281
Fragments arranged by size:
3281 1572 602
ApaLI G'TGCA_C
Cuts at: 3512 4668 3512
Size: 1156 4299
ApoI r'AATT_y
Cuts at: 1493 2481 2764 2990 3142 5251 1493
Size: 988 283 226 152 2109 1697
Fragments arranged by size:
2109 1697 988 283 226 152
AscI GG'CGCG_CC
Cuts at: 2373 2373
Size: 5455
AseI AT'TA_AT
Cuts at: 160 1032 4009 160
Size: 872 2977 1606
Fragments arranged by size:
2977 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5455
AvaI C'yCGr_G
Cuts at: 1870 1943 2329 2405 2780 1870
Size: 73 386 76 375 4545
Fragments arranged by size:
4545 386 375 76 73
BamHI G'GATC_C
Cuts at: 2785 3152 2785
Size: 367 5088
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2183 2194 2283 2308 2776
Size: 325 217 627 396 11 89 25 468
Cuts at: 2776 4231 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 468 396 325 217 89
25 11
BanII G_rGCy'C
Cuts at: 1391 2111 2207 2774 1391
Size: 720 96 567 4072
Fragments arranged by size:
4072 720 567 96
BbeI G_GCGC'C
Cuts at: 2187 2198 2312 2187
Size: 11 114 5330
Fragments arranged by size:
5330 114 11
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4484
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3646 3680 1903
Size: 34 1709 34 3678
Fragments arranged by size:
3678 1709 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3347 4557 3347
Size: 1210 4245
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5455
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2192 4066 136
Size: 107 122 71 631 605 520 1874 1525
Fragments arranged by size:
1874 1525 631 605 520 122 107 71
BglII A'GATC_T
Cuts at: 5450 5450
Size: 5455
BlpI GC'TnA_GC
Cuts at: 2024 2201 2872 2024
Size: 177 671 4607
Fragments arranged by size:
4607 671 177
Bme1580I G_kGCm'C
Cuts at: 1790 2402 3516 4672 1790
Size: 612 1114 1156 2573
Fragments arranged by size:
2573 1156 1114 612
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5455
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3186 4144 442
Size: 416 53 83 399 24 1769 958 1753
Fragments arranged by size:
1769 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5455
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2164 2164
Size: 5455
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2061 2159 2321 2618 4115 1239
Size: 822 98 162 297 1497 2579
Fragments arranged by size:
2579 1497 822 297 162 98
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3580 4445 4743 3580
Size: 865 298 4292
Fragments arranged by size:
4292 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4118 882
Size: 617 2619 2219
Fragments arranged by size:
2619 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3246 493
Size: 711 2042 2702
Fragments arranged by size:
2702 2042 711
BsaBI GATnn'nnATC
Cuts at: 5449 5449
Size: 5455
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2184 2195 2262 2309
Size: 53 83 186 936 651 11 67 47
Cuts at: 2309 3262 3644 4279 275
Size: 953 382 635 1451
Fragments arranged by size:
1451 953 936 651 635 382 186 83
67 53 47 11
BsaWI w'CCGG_w
Cuts at: 3888 4286 4560 4707 5118 3888
Size: 398 274 147 411 4225
Fragments arranged by size:
4225 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2603 2633 1178
Size: 30 165 30 1200 30 4000
Fragments arranged by size:
4000 1200 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2657 1186
Size: 1471 3984
BseYI C'CCAG_C
Cuts at: 2368 4658 5154 2368
Size: 2290 496 2669
Fragments arranged by size:
2669 2290 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2173 2528 1163
Size: 573 437 355 4090
Fragments arranged by size:
4090 573 437 355
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2274 2905 3666 4694 1540
Size: 275 186 273 631 761 1028 2301
Fragments arranged by size:
2301 1028 761 631 275 273 186
BsiHKAI G_wGCw'C
Cuts at: 2111 2136 3516 3601 4672 2111
Size: 25 1380 85 1071 2894
Fragments arranged by size:
2894 1380 1071 85 25
BsmI GAATG_Cn'
Cuts at: 2966 3059 2966
Size: 93 5362
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2111 2136 2207 2402 2774 3516 3601
Size: 399 321 25 71 195 372 742 85
Cuts at: 3601 4672 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 372 321 195 85
71 25
BspEI T'CCGG_A
Cuts at: 5118 5118
Size: 5455
BspHI T'CATG_A
Cuts at: 3344 5074 3344
Size: 1730 3725
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2271 2810 844
Size: 1427 539 3489
Fragments arranged by size:
3489 1427 539
BsrBI CCG'CTC
Cuts at: 1394 1823 3342 1394
Size: 429 1519 3507
Fragments arranged by size:
3507 1519 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3950 4124 65
Size: 1183 2702 174 1396
Fragments arranged by size:
2702 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2173 2269 2305 2709 4099 4286 1700
Size: 110 363 96 36 404 1390 187 2869
Fragments arranged by size:
2869 1390 404 363 187 110 96 36
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5455
BssHII G'CGCG_C
Cuts at: 1925 2373 1925
Size: 448 5007
BssSI C'ACGA_G
Cuts at: 2057 2149 3515 4527 2057
Size: 92 1366 1012 2985
Fragments arranged by size:
2985 1366 1012 92
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5455
BstBI TT'CG_AA
Cuts at: 4259 4259
Size: 5455
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5455
BstYI r'GATC_y
Cuts at: 1972 2785 3152 3537 3554 5006 5092 5104 5450
Size: 813 367 385 17 1452 86 12 346
Cuts at: 5450 1972
Size: 1977
Fragments arranged by size:
1977 1452 813 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4903
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4716
Fragments arranged by size:
4716 683 56
BtsI GCAGTG_nn'
Cuts at: 2971 3765 3785 2971
Size: 794 20 4641
Fragments arranged by size:
4641 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5455
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5420
DraI TTT'AAA
Cuts at: 2759 2759
Size: 5455
DraIII CAC_nnn'GTG
Cuts at: 2656 2656
Size: 5455
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4462 809
Size: 507 3146 1802
Fragments arranged by size:
3146 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2095 2271 2658
Size: 54 1425 50 275 18 265 176 387
Cuts at: 2658 2902 3791 5420 8
Size: 244 889 1629 43
Fragments arranged by size:
1629 1425 889 387 275 265 244 176
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2271 2902 1537
Size: 275 459 631 4090
Fragments arranged by size:
4090 631 459 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2560 3385 1353
Size: 1207 825 3423
Fragments arranged by size:
3423 1207 825
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2372 2521 2728 4042 4416 4562
Size: 1064 138 783 149 207 1314 374 146
Cuts at: 4562 387
Size: 1280
Fragments arranged by size:
1314 1280 1064 783 374 207 149 146
138
EcoICRI GAG'CTC
Cuts at: 2109 2109
Size: 5455
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2061 2159 2321 2618 3518 4115 4902
Size: 393 429 98 162 297 900 597 787
Cuts at: 4902 5170 1239
Size: 268 1524
Fragments arranged by size:
1524 900 787 597 429 393 297 268
162 98
EcoRI G'AATT_C
Cuts at: 2764 3142 2764
Size: 378 5077
FspI TGC'GCA
Cuts at: 3961 3961
Size: 5455
HaeII r_GCGC'y
Cuts at: 1678 2174 2187 2198 2312 2572 4602 1678
Size: 496 13 11 114 260 2030 2531
Fragments arranged by size:
2531 2030 496 260 114 13 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2038 2070 2500 2532 4096
Size: 32 135 32 466 32 430 32 1564
Cuts at: 4096 4128 4170 4202 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1564 466 430 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2041 2799 3045 669
Size: 988 281 103 758 246 3079
Fragments arranged by size:
3079 988 758 281 246 103
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5455
HpaI GTT'AAC
Cuts at: 3045 3045
Size: 5455
KasI G'GCGC_C
Cuts at: 2183 2194 2308 2183
Size: 11 114 5330
Fragments arranged by size:
5330 114 11
KpnI G_GTAC'C
Cuts at: 2287 2780 2287
Size: 493 4962
MfeI C'AATT_G
Cuts at: 3054 3054
Size: 5455
MluI A'CGCG_T
Cuts at: 2211 5331 2211
Size: 3120 2335
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4569 4753 1305
Size: 3264 184 2007
Fragments arranged by size:
3264 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2097 2660 10
Size: 54 1425 343 265 563 2805
Fragments arranged by size:
2805 1425 563 343 265 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2061 3413 3772 3931 518
Size: 648 895 1352 359 159 2042
Fragments arranged by size:
2042 1352 895 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2200 2365 2871 3548 4696 4941 1606
Size: 594 165 506 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 594 506 245 165
NaeI GCC'GGC
Cuts at: 2271 2307 2271
Size: 36 5419
NarI GG'CG_CC
Cuts at: 2184 2195 2309 2184
Size: 11 114 5330
Fragments arranged by size:
5330 114 11
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4903
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5455
NgoMIV G'CCGG_C
Cuts at: 2269 2305 2269
Size: 36 5419
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5455
NotI GC'GGCC_GC
Cuts at: 2902 2902
Size: 5455
NspI r_CATG'y
Cuts at: 1132 1229 2813 4358 1132
Size: 97 1584 1545 2229
Fragments arranged by size:
2229 1584 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2257 1348
Size: 909 4546
PciI A'CATG_T
Cuts at: 4354 4354
Size: 5455
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2596 1108
Size: 1488 3967
PfoI T'CCnGG_A
Cuts at: 2216 2558 2216
Size: 342 5113
PmeI GTTT'AAAC
Cuts at: 2759 2759
Size: 5455
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2603 2635 3524 3556 5037 5069 2603
Size: 32 889 32 1481 32 2989
Fragments arranged by size:
2989 1481 889 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5455
PsiI TTA'TAA
Cuts at: 3025 3025
Size: 5455
PstI C_TGCA'G
Cuts at: 830 2807 830
Size: 1977 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5455
SacI G_AGCT'C
Cuts at: 2111 2111
Size: 5455
SalI G'TCGA_C
Cuts at: 1936 2039 2797 1936
Size: 103 758 4594
Fragments arranged by size:
4594 758 103
SapI GCTCTTCn'nnn_
Cuts at: 2560 2560
Size: 5455
SbfI CC_TGCA'GG
Cuts at: 2807 2807
Size: 5455
ScaI AGT'ACT
Cuts at: 3703 5415 3703
Size: 1712 3743
SfcI C'TryA_G
Cuts at: 826 1045 2803 3938 4619 4810 826
Size: 219 1758 1135 681 191 1471
Fragments arranged by size:
1758 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2185 2196 2310 2185
Size: 11 114 5330
Fragments arranged by size:
5330 114 11
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5455
SgrAI Cr'CCGG_yG
Cuts at: 1700 2173 1700
Size: 473 4982
SmaI CCC'GGG
Cuts at: 1872 2331 2782 1872
Size: 459 451 4545
Fragments arranged by size:
4545 459 451
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3559 4460 4722 820
Size: 197 926 1616 901 262 1553
Fragments arranged by size:
1616 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3246 493
Size: 2753 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5455
SphI G_CATG'C
Cuts at: 1132 2813 1132
Size: 1681 3774
SspI AAT'ATT
Cuts at: 5 52 3379 5
Size: 47 3327 2081
Fragments arranged by size:
3327 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5455
StyI C'CwwG_G
Cuts at: 513 1065 2894 513
Size: 552 1829 3074
Fragments arranged by size:
3074 1829 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3482 3499 3652 3837 4282 325
Size: 1255 1902 17 153 185 445 1498
Fragments arranged by size:
1902 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2409 3701 5413
Size: 274 80 33 51 1443 432 1292 1712
Cuts at: 5413 96
Size: 138
Fragments arranged by size:
1712 1443 1292 432 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5415 5447 5415
Size: 32 5423
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5455
XbaI T'CTAG_A
Cuts at: 2791 2791
Size: 5455
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5455
XmaI C'CCGG_G
Cuts at: 1870 2329 2780 1870
Size: 459 451 4545
Fragments arranged by size:
4545 459 451
XmnI GAAnn'nnTTC
Cuts at: 3584 3584
Size: 5455
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3263 276
Size: 53 83 186 936 1729 2468
Fragments arranged by size:
2468 1729 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI ApaI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI
BsmBI BstXI BstZ17I Bsu36I EcoNI EcoO109I EcoRV FalI
FseI FspAI NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI
SrfI SwaI XcmI