Restriction map for
pFN21AB8907
(Circular) (Six-base) MAPSORT of: pFN21AB8907.seq Check: 5259 from: 1 to: 5422
pFN21AB8907 5422 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3232 278
Size: 53 83 186 936 1696 2468
Fragments arranged by size:
2468 1696 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2765 1937
Size: 828 4594
Acc65I G'GTAC_C
Cuts at: 2743 2743
Size: 5422
AclI AA'CG_TT
Cuts at: 1563 3549 3922 1563
Size: 1986 373 3063
Fragments arranged by size:
3063 1986 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2097 2210 3485 4869 5137 1632
Size: 465 113 1275 1384 268 1917
Fragments arranged by size:
1917 1384 1275 465 268 113
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5225
AflIII A'CryG_T
Cuts at: 1203 4321 5298 1203
Size: 3118 977 1327
Fragments arranged by size:
3118 1327 977
AgeI A'CCGG_T
Cuts at: 4253 4253
Size: 5422
AhdI GACnn_n'nnGTC
Cuts at: 4151 4151
Size: 5422
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5422
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5422
AlwNI CAG_nnn'CTG
Cuts at: 2242 3165 4737 2242
Size: 923 1572 2927
Fragments arranged by size:
2927 1572 923
ApaI G_GGCC'C
Cuts at: 2486 2486
Size: 5422
ApaLI G'TGCA_C
Cuts at: 3479 4635 3479
Size: 1156 4266
ApoI r'AATT_y
Cuts at: 1493 2731 2957 3109 5218 1493
Size: 1238 226 152 2109 1697
Fragments arranged by size:
2109 1697 1238 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3976 160
Size: 872 2944 1606
Fragments arranged by size:
2944 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5422
AvaI C'yCGr_G
Cuts at: 1870 1943 2747 1870
Size: 73 804 4545
Fragments arranged by size:
4545 804 73
BamHI G'GATC_C
Cuts at: 2752 3119 2752
Size: 367 5055
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2126 2743 4198 618
Size: 325 217 627 339 617 1455 1842
Fragments arranged by size:
1842 1455 627 617 339 325 217
BanII G_rGCy'C
Cuts at: 1391 2360 2486 2497 2741 1391
Size: 969 126 11 244 4072
Fragments arranged by size:
4072 969 244 126 11
BbeI G_GCGC'C
Cuts at: 2130 2130
Size: 5422
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2075 928
Size: 971 176 4275
Fragments arranged by size:
4275 971 176
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3613 3647 1903
Size: 34 1676 34 3678
Fragments arranged by size:
3678 1676 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3314 4524 3314
Size: 1210 4212
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5422
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2349 4033 136
Size: 107 122 71 631 605 677 1684 1525
Fragments arranged by size:
1684 1525 677 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5417 5417
Size: 5422
BlpI GC'TnA_GC
Cuts at: 2839 2839
Size: 5422
Bme1580I G_kGCm'C
Cuts at: 1790 2486 3483 4639 1790
Size: 696 997 1156 2573
Fragments arranged by size:
2573 1156 997 696
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5422
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2524 3153 4111
Size: 416 53 83 399 24 1107 629 958
Cuts at: 4111 442
Size: 1753
Fragments arranged by size:
1753 1107 958 629 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5422
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4082 1239
Size: 2843 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2353 2608 3547 4412 4710 2353
Size: 255 939 865 298 3065
Fragments arranged by size:
3065 939 865 298 255
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4085 882
Size: 617 2586 2219
Fragments arranged by size:
2586 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3213 493
Size: 711 2009 2702
Fragments arranged by size:
2702 2009 711
BsaBI GATnn'nnATC
Cuts at: 5416 5416
Size: 5422
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2030 2127 2147 3229
Size: 53 83 186 936 497 97 20 1082
Cuts at: 3229 3611 4246 275
Size: 382 635 1451
Fragments arranged by size:
1451 1082 936 635 497 382 186 97
83 53 20
BsaWI w'CCGG_w
Cuts at: 3855 4253 4527 4674 5085 3855
Size: 398 274 147 411 4192
Fragments arranged by size:
4192 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5197
Fragments arranged by size:
5197 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2303 1186
Size: 1117 4305
BseYI C'CCAG_C
Cuts at: 2113 2382 2462 2665 4625 5121 2113
Size: 269 80 203 1960 496 2414
Fragments arranged by size:
2414 1960 496 269 203 80
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4849
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2416 2872 3633 4661 1540
Size: 275 186 415 456 761 1028 2301
Fragments arranged by size:
2301 1028 761 456 415 275 186
BsiHKAI G_wGCw'C
Cuts at: 3483 3568 4639 3483
Size: 85 1071 4266
Fragments arranged by size:
4266 1071 85
BsmI GAATG_Cn'
Cuts at: 2933 3026 2933
Size: 93 5329
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2360 2486 2497 2741 3483 3568 4639
Size: 399 570 126 11 244 742 85 1071
Cuts at: 4639 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 570 399 244 126 85
11
BspEI T'CCGG_A
Cuts at: 5085 5085
Size: 5422
BspHI T'CATG_A
Cuts at: 3311 5041 3311
Size: 1730 3692
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2046 2217 2777 844
Size: 1202 171 560 3489
Fragments arranged by size:
3489 1202 560 171
BsrBI CCG'CTC
Cuts at: 1394 1823 2293 2367 3309 1394
Size: 429 470 74 942 3507
Fragments arranged by size:
3507 942 470 429 74
BsrDI GCAATG_nn'
Cuts at: 65 1248 2311 3917 4091 65
Size: 1183 1063 1606 174 1396
Fragments arranged by size:
1606 1396 1183 1063 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2156 2415 4066 4253 1700
Size: 110 346 259 1651 187 2869
Fragments arranged by size:
2869 1651 346 259 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5422
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5422
BssSI C'ACGA_G
Cuts at: 3482 4494 3482
Size: 1012 4410
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5422
BstBI TT'CG_AA
Cuts at: 4226 4226
Size: 5422
BstEII G'GTnAC_C
Cuts at: 1184 2245 1184
Size: 1061 4361
BstXI CCAn_nnnn'nTGG
Cuts at: 2470 2470
Size: 5422
BstYI r'GATC_y
Cuts at: 1972 2229 2752 3119 3504 3521 4973 5059 5071
Size: 257 523 367 385 17 1452 86 12
Cuts at: 5071 5417 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 523 385 367 346 257 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2016 2089 513
Size: 552 939 12 73 3846
Fragments arranged by size:
3846 939 552 73 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4683
Fragments arranged by size:
4683 683 56
BtsI GCAGTG_nn'
Cuts at: 2277 2702 2938 3732 3752 2277
Size: 425 236 794 20 3947
Fragments arranged by size:
3947 794 425 236 20
ClaI AT'CG_AT
Cuts at: 1554 2220 1554
Size: 666 4756
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5387
DraI TTT'AAA
Cuts at: 2726 2726
Size: 5422
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2020 4429 809
Size: 507 704 2409 1802
Fragments arranged by size:
2409 1802 704 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2158 2869 3758
Size: 54 1425 50 275 18 328 711 889
Cuts at: 3758 5387 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 711 328 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2869 1537
Size: 275 1057 4090
Fragments arranged by size:
4090 1057 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3352 1353
Size: 1999 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4009 4383 4529 387
Size: 1064 138 2420 374 146 1280
Fragments arranged by size:
2420 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2097 2210 3485 4082 4869 5137 1239
Size: 393 465 113 1275 597 787 268 1524
Fragments arranged by size:
1524 1275 787 597 465 393 268 113
EcoO109I rG'GnC_Cy
Cuts at: 2482 2482
Size: 5422
EcoRI G'AATT_C
Cuts at: 2731 3109 2731
Size: 378 5044
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2686 2686
Size: 5422
FspI TGC'GCA
Cuts at: 3928 3928
Size: 5422
HaeII r_GCGC'y
Cuts at: 1678 2130 2189 4569 1678
Size: 452 59 2380 2531
Fragments arranged by size:
2531 2380 452 59
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2414 2446 4063 4095 4137
Size: 32 135 32 842 32 1617 32 42
Cuts at: 4137 4169 1373
Size: 32 2626
Fragments arranged by size:
2626 1617 842 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2304 2766 3012 669
Size: 988 281 366 462 246 3079
Fragments arranged by size:
3079 988 462 366 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5422
HpaI GTT'AAC
Cuts at: 3012 3012
Size: 5422
KasI G'GCGC_C
Cuts at: 2126 2126
Size: 5422
KpnI G_GTAC'C
Cuts at: 2747 2747
Size: 5422
MfeI C'AATT_G
Cuts at: 3021 3021
Size: 5422
MluI A'CGCG_T
Cuts at: 5298 5298
Size: 5422
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2265 4536 4720 1305
Size: 960 2271 184 2007
Fragments arranged by size:
2271 2007 960 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3600
Fragments arranged by size:
3600 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2222 3380 3739 3898 518
Size: 648 1056 1158 359 159 2042
Fragments arranged by size:
2042 1158 1056 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2091 2242 2382 2838 3515 4663 4908 1606
Size: 485 151 140 456 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 485 456 245 151 140
NarI GG'CG_CC
Cuts at: 2127 2127
Size: 5422
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3931
Fragments arranged by size:
3931 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5422
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5422
NotI GC'GGCC_GC
Cuts at: 2869 2869
Size: 5422
NspI r_CATG'y
Cuts at: 1132 1229 2780 4325 1132
Size: 97 1551 1545 2229
Fragments arranged by size:
2229 1551 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2141 2609 1348
Size: 793 468 4161
Fragments arranged by size:
4161 793 468
PciI A'CATG_T
Cuts at: 4321 4321
Size: 5422
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2167 1108
Size: 1059 4363
PfoI T'CCnGG_A
Cuts at: 2445 2445
Size: 5422
PmeI GTTT'AAAC
Cuts at: 2726 2726
Size: 5422
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3491 3523 5004 5036 3491
Size: 32 1481 32 3877
Fragments arranged by size:
3877 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5422
PsiI TTA'TAA
Cuts at: 2992 2992
Size: 5422
PspOMI G'GGCC_C
Cuts at: 2482 2482
Size: 5422
PstI C_TGCA'G
Cuts at: 830 2238 2774 830
Size: 1408 536 3478
Fragments arranged by size:
3478 1408 536
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5422
PvuII CAG'CTG
Cuts at: 2242 2382 2242
Size: 140 5282
SacII CC_GC'GG
Cuts at: 2092 2092
Size: 5422
SalI G'TCGA_C
Cuts at: 1936 2764 1936
Size: 828 4594
SbfI CC_TGCA'GG
Cuts at: 2774 2774
Size: 5422
ScaI AGT'ACT
Cuts at: 3670 5382 3670
Size: 1712 3710
SfcI C'TryA_G
Cuts at: 826 1045 2234 2770 3905 4586 4777 826
Size: 219 1189 536 1135 681 191 1471
Fragments arranged by size:
1471 1189 1135 681 536 219 191
SfoI GGC'GCC
Cuts at: 2128 2128
Size: 5422
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5422
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5422
SmaI CCC'GGG
Cuts at: 1872 2749 1872
Size: 877 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2368 2587 3526 4427 4689 820
Size: 197 926 425 219 939 901 262 1553
Fragments arranged by size:
1553 939 926 901 425 262 219 197
SnaBI TAC'GTA
Cuts at: 493 3213 493
Size: 2720 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5422
SphI G_CATG'C
Cuts at: 1132 2780 1132
Size: 1648 3774
SspI AAT'ATT
Cuts at: 5 52 3346 5
Size: 47 3294 2081
Fragments arranged by size:
3294 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5422
StyI C'CwwG_G
Cuts at: 513 1065 2004 2861 513
Size: 552 939 857 3074
Fragments arranged by size:
3074 939 857 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3449 3466 3619 3804 4249 325
Size: 1255 1869 17 153 185 445 1498
Fragments arranged by size:
1869 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3668 5380 96
Size: 274 80 33 51 1443 1691 1712 138
Fragments arranged by size:
1712 1691 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5382 5414 5382
Size: 32 5390
Tth111I GACn'n_nGTC
Cuts at: 1316 2426 1316
Size: 1110 4312
XbaI T'CTAG_A
Cuts at: 2758 2758
Size: 5422
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2416 2554 2416
Size: 138 5284
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5422
XmaI C'CCGG_G
Cuts at: 1870 2747 1870
Size: 877 4545
XmnI GAAnn'nnTTC
Cuts at: 3551 3551
Size: 5422
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3230 276
Size: 53 83 186 936 1696 2468
Fragments arranged by size:
2468 1696 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI
PacI PmlI PpuMI PspXI PsrI RsrII SacI SanDI
SapI SexAI SfiI SrfI SwaI