Restriction map for
pFN21AB8906
(Circular) (Six-base) MAPSORT of: pFN21AB8906.seq Check: 9161 from: 1 to: 5422
pFN21AB8906 5422 bp
With 182 enzymes: SgfI *
December 8, 2014 13:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2085 2085
Size: 5422
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3232 278
Size: 53 83 186 936 1696 2468
Fragments arranged by size:
2468 1696 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2765 1937
Size: 828 4594
Acc65I G'GTAC_C
Cuts at: 2325 2743 2325
Size: 418 5004
AclI AA'CG_TT
Cuts at: 1563 3549 3922 1563
Size: 1986 373 3063
Fragments arranged by size:
3063 1986 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2441 3485 4869 5137 1632
Size: 809 1044 1384 268 1917
Fragments arranged by size:
1917 1384 1044 809 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5225
AflIII A'CryG_T
Cuts at: 1203 4321 5298 1203
Size: 3118 977 1327
Fragments arranged by size:
3118 1327 977
AgeI A'CCGG_T
Cuts at: 4253 4253
Size: 5422
AhdI GACnn_n'nnGTC
Cuts at: 4151 4151
Size: 5422
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5422
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5422
AlwNI CAG_nnn'CTG
Cuts at: 2293 3165 4737 2293
Size: 872 1572 2978
Fragments arranged by size:
2978 1572 872
ApaLI G'TGCA_C
Cuts at: 3479 4635 3479
Size: 1156 4266
ApoI r'AATT_y
Cuts at: 1493 2731 2957 3109 5218 1493
Size: 1238 226 152 2109 1697
Fragments arranged by size:
2109 1697 1238 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3976 160
Size: 872 2944 1606
Fragments arranged by size:
2944 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5422
AvaI C'yCGr_G
Cuts at: 1870 1943 2057 2339 2747 1870
Size: 73 114 282 408 4545
Fragments arranged by size:
4545 408 282 114 73
BamHI G'GATC_C
Cuts at: 2752 3119 2752
Size: 367 5055
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2325 2515 2600 2743 4198
Size: 325 217 627 538 190 85 143 1455
Cuts at: 4198 618
Size: 1842
Fragments arranged by size:
1842 1455 627 538 325 217 190 143
85
BanII G_rGCy'C
Cuts at: 1391 2240 2741 1391
Size: 849 501 4072
Fragments arranged by size:
4072 849 501
BbeI G_GCGC'C
Cuts at: 2604 2604
Size: 5422
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4451
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3613 3647 1903
Size: 34 1676 34 3678
Fragments arranged by size:
3678 1676 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3314 4524 3314
Size: 1210 4212
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5422
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4033 136
Size: 107 122 71 631 605 2361 1525
Fragments arranged by size:
2361 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2391 5417 2391
Size: 3026 2396
BlpI GC'TnA_GC
Cuts at: 2839 2839
Size: 5422
Bme1580I G_kGCm'C
Cuts at: 1790 3483 4639 1790
Size: 1693 1156 2573
Fragments arranged by size:
2573 1693 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5422
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2728 3153 4111
Size: 416 53 83 399 24 1311 425 958
Cuts at: 4111 442
Size: 1753
Fragments arranged by size:
1753 1311 958 425 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5422
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2201 2315 2421 2592 4082 1239
Size: 962 114 106 171 1490 2579
Fragments arranged by size:
2579 1490 962 171 114 106
Bpu10I CC'TnA_GC
Cuts at: 2024 2024
Size: 5422
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3547 4412 4710 3547
Size: 865 298 4259
Fragments arranged by size:
4259 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2177 2713 4085 882
Size: 617 678 536 1372 2219
Fragments arranged by size:
2219 1372 678 617 536
BsaAI yAC'GTr
Cuts at: 493 1204 3213 493
Size: 711 2009 2702
Fragments arranged by size:
2702 2009 711
BsaBI GATnn'nnATC
Cuts at: 5416 5416
Size: 5422
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2020 2244 2601 3229
Size: 53 83 186 936 487 224 357 628
Cuts at: 3229 3611 4246 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 635 628 487 382 357 224
186 83 53
BsaWI w'CCGG_w
Cuts at: 3855 4253 4527 4674 5085 3855
Size: 398 274 147 411 4192
Fragments arranged by size:
4192 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5197
Fragments arranged by size:
5197 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5422
BseYI C'CCAG_C
Cuts at: 2109 4625 5121 2109
Size: 2516 496 2410
Fragments arranged by size:
2516 2410 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2465 1163
Size: 573 729 4120
Fragments arranged by size:
4120 729 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2213 2872 3633 4661 1540
Size: 275 186 212 659 761 1028 2301
Fragments arranged by size:
2301 1028 761 659 275 212 186
BsiHKAI G_wGCw'C
Cuts at: 3483 3568 4639 3483
Size: 85 1071 4266
Fragments arranged by size:
4266 1071 85
BsmI GAATG_Cn'
Cuts at: 2933 3026 2933
Size: 93 5329
BsmBI CGTCTCn'nnnn_
Cuts at: 2235 2235
Size: 5422
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2240 2741 3483 3568 4639 1391
Size: 399 450 501 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 501 450 399 85
BspEI T'CCGG_A
Cuts at: 5085 5085
Size: 5422
BspHI T'CATG_A
Cuts at: 3311 5041 3311
Size: 1730 3692
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2085 2374 2777 844
Size: 1241 289 403 3489
Fragments arranged by size:
3489 1241 403 289
BsrBI CCG'CTC
Cuts at: 1394 1823 2305 3309 1394
Size: 429 482 1004 3507
Fragments arranged by size:
3507 1004 482 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3917 4091 65
Size: 1183 2669 174 1396
Fragments arranged by size:
2669 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2518 4066 4253 1700
Size: 110 708 1548 187 2869
Fragments arranged by size:
2869 1548 708 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5422
BssHII G'CGCG_C
Cuts at: 1925 2425 1925
Size: 500 4922
BssSI C'ACGA_G
Cuts at: 3482 4494 3482
Size: 1012 4410
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2077 794
Size: 1283 4139
BstBI TT'CG_AA
Cuts at: 4226 4226
Size: 5422
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5422
BstYI r'GATC_y
Cuts at: 1972 2391 2752 3119 3504 3521 4973 5059 5071
Size: 419 361 367 385 17 1452 86 12
Cuts at: 5071 5417 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 419 385 367 361 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2580 513
Size: 552 939 576 3355
Fragments arranged by size:
3355 939 576 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2393 487
Size: 683 56 1167 3516
Fragments arranged by size:
3516 1167 683 56
BtsI GCAGTG_nn'
Cuts at: 2938 3732 3752 2938
Size: 794 20 4608
Fragments arranged by size:
4608 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5422
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5387
DraI TTT'AAA
Cuts at: 2726 2726
Size: 5422
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4429 809
Size: 507 3113 1802
Fragments arranged by size:
3113 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2149 2210
Size: 54 1425 50 275 18 177 142 61
Cuts at: 2210 2869 3758 5387 8
Size: 659 889 1629 43
Fragments arranged by size:
1629 1425 889 659 275 177 142 61
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2210 2869 1537
Size: 275 398 659 4090
Fragments arranged by size:
4090 659 398 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2675 3352 1353
Size: 1322 677 3423
Fragments arranged by size:
3423 1322 677
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2461 2462 4009 4383 4529 387
Size: 1064 138 872 1 1547 374 146 1280
Fragments arranged by size:
1547 1280 1064 872 374 146 138 1
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2201 2315 2421 2441 2592 3485 4082
Size: 393 569 114 106 20 151 893 597
Cuts at: 4082 4869 5137 1239
Size: 787 268 1524
Fragments arranged by size:
1524 893 787 597 569 393 268 151
114 106 20
EcoRI G'AATT_C
Cuts at: 2731 3109 2731
Size: 378 5044
FspI TGC'GCA
Cuts at: 2039 3928 2039
Size: 1889 3533
HaeII r_GCGC'y
Cuts at: 1678 2311 2604 2663 4569 1678
Size: 633 293 59 1906 2531
Fragments arranged by size:
2531 1906 633 293 59
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4063 4095 4137 4169 1373
Size: 32 135 32 2491 32 42 32 2626
Fragments arranged by size:
2626 2491 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2766 3012 669
Size: 988 281 828 246 3079
Fragments arranged by size:
3079 988 828 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5422
HpaI GTT'AAC
Cuts at: 3012 3012
Size: 5422
KasI G'GCGC_C
Cuts at: 2600 2600
Size: 5422
KpnI G_GTAC'C
Cuts at: 2329 2747 2329
Size: 418 5004
MfeI C'AATT_G
Cuts at: 3021 3021
Size: 5422
MluI A'CGCG_T
Cuts at: 5298 5298
Size: 5422
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2280 4536 4720 1305
Size: 975 2256 184 2007
Fragments arranged by size:
2256 2007 975 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 3423
Fragments arranged by size:
3423 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3380 3739 3898 518
Size: 648 2214 359 159 2042
Fragments arranged by size:
2214 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2209 2323 2353 2582 2838 3515 4663 4908
Size: 603 114 30 229 256 677 1148 245
Cuts at: 4908 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 603 256 245 229 114
30
NarI GG'CG_CC
Cuts at: 2601 2601
Size: 5422
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3931
Fragments arranged by size:
3931 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5422
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5422
NotI GC'GGCC_GC
Cuts at: 2869 2869
Size: 5422
NruI TCG'CGA
Cuts at: 2532 2532
Size: 5422
NspI r_CATG'y
Cuts at: 1132 1229 2780 4325 1132
Size: 97 1551 1545 2229
Fragments arranged by size:
2229 1551 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5422
PciI A'CATG_T
Cuts at: 4321 4321
Size: 5422
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5422
PfoI T'CCnGG_A
Cuts at: 2711 2711
Size: 5422
PmeI GTTT'AAAC
Cuts at: 2726 2726
Size: 5422
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3491 3523 5004 5036 3491
Size: 32 1481 32 3877
Fragments arranged by size:
3877 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5422
PsiI TTA'TAA
Cuts at: 2992 2992
Size: 5422
PstI C_TGCA'G
Cuts at: 830 2774 830
Size: 1944 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5422
PvuII CAG'CTG
Cuts at: 2353 2353
Size: 5422
SacII CC_GC'GG
Cuts at: 2583 2583
Size: 5422
SalI G'TCGA_C
Cuts at: 1936 2764 1936
Size: 828 4594
SbfI CC_TGCA'GG
Cuts at: 2774 2774
Size: 5422
ScaI AGT'ACT
Cuts at: 3670 5382 3670
Size: 1712 3710
SfcI C'TryA_G
Cuts at: 826 1045 2770 3905 4586 4777 826
Size: 219 1725 1135 681 191 1471
Fragments arranged by size:
1725 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2602 2602
Size: 5422
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5422
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5422
SmaI CCC'GGG
Cuts at: 1872 2749 1872
Size: 877 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3526 4427 4689 820
Size: 197 926 1583 901 262 1553
Fragments arranged by size:
1583 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3213 493
Size: 2720 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5422
SphI G_CATG'C
Cuts at: 1132 2780 1132
Size: 1648 3774
SspI AAT'ATT
Cuts at: 5 52 3346 5
Size: 47 3294 2081
Fragments arranged by size:
3294 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5422
StyI C'CwwG_G
Cuts at: 513 1065 2004 2280 2861 513
Size: 552 939 276 581 3074
Fragments arranged by size:
3074 939 581 552 276
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2636 3449 3466 3619 3804 4249 325
Size: 1255 1056 813 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1056 813 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3668 5380 96
Size: 274 80 33 51 1443 1691 1712 138
Fragments arranged by size:
1712 1691 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2144 2176 2678 2710 5382 5414 2144
Size: 32 502 32 2672 32 2152
Fragments arranged by size:
2672 2152 502 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5422
XbaI T'CTAG_A
Cuts at: 2047 2758 2047
Size: 711 4711
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5422
XmaI C'CCGG_G
Cuts at: 1870 2747 1870
Size: 877 4545
XmnI GAAnn'nnTTC
Cuts at: 3551 3551
Size: 5422
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3230 276
Size: 53 83 186 936 1696 2468
Fragments arranged by size:
2468 1696 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NruI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SacII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NsiI
PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI
SanDI SapI SexAI SfiI SrfI SwaI XcmI