Restriction map for
pFN21AB8900
(Circular) (Six-base) MAPSORT of: pFN21AB8900.seq Check: 4310 from: 1 to: 5404
pFN21AB8900 5404 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2340 2340
Size: 5404
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3214 278
Size: 53 83 186 936 1678 2468
Fragments arranged by size:
2468 1678 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2747 1937
Size: 810 4594
Acc65I G'GTAC_C
Cuts at: 2725 2725
Size: 5404
AclI AA'CG_TT
Cuts at: 1563 3531 3904 1563
Size: 1968 373 3063
Fragments arranged by size:
3063 1968 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3467 4851 5119 1632
Size: 1835 1384 268 1917
Fragments arranged by size:
1917 1835 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5207
AflIII A'CryG_T
Cuts at: 1203 2166 4303 5280 1203
Size: 963 2137 977 1327
Fragments arranged by size:
2137 1327 977 963
AgeI A'CCGG_T
Cuts at: 4235 4235
Size: 5404
AhdI GACnn_n'nnGTC
Cuts at: 4133 4133
Size: 5404
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5404
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5404
AlwNI CAG_nnn'CTG
Cuts at: 3147 4719 3147
Size: 1572 3832
ApaLI G'TGCA_C
Cuts at: 3461 4617 3461
Size: 1156 4248
ApoI r'AATT_y
Cuts at: 1493 2713 2939 3091 5200 1493
Size: 1220 226 152 2109 1697
Fragments arranged by size:
2109 1697 1220 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3958 160
Size: 872 2926 1606
Fragments arranged by size:
2926 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5404
AvaI C'yCGr_G
Cuts at: 1870 1943 2126 2383 2729 1870
Size: 73 183 257 346 4545
Fragments arranged by size:
4545 346 257 183 73
AvrII C'CTAG_G
Cuts at: 2594 2594
Size: 5404
BamHI G'GATC_C
Cuts at: 2734 3101 2734
Size: 367 5037
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2387 2678 2725 4180 618
Size: 325 217 627 600 291 47 1455 1842
Fragments arranged by size:
1842 1455 627 600 325 291 217 47
BanII G_rGCy'C
Cuts at: 1391 2117 2186 2384 2723 1391
Size: 726 69 198 339 4072
Fragments arranged by size:
4072 726 339 198 69
BbeI G_GCGC'C
Cuts at: 2391 2391
Size: 5404
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4433
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3595 3629 1903
Size: 34 1658 34 3678
Fragments arranged by size:
3678 1658 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3296 4506 3296
Size: 1210 4194
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5404
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4015 136
Size: 107 122 71 631 605 2343 1525
Fragments arranged by size:
2343 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2238 5399 2238
Size: 3161 2243
BlpI GC'TnA_GC
Cuts at: 2190 2821 2190
Size: 631 4773
Bme1580I G_kGCm'C
Cuts at: 1790 2683 3465 4621 1790
Size: 893 782 1156 2573
Fragments arranged by size:
2573 1156 893 782
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5404
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3135 4093 442
Size: 416 53 83 399 24 1718 958 1753
Fragments arranged by size:
1753 1718 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5404
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2205 2205
Size: 5404
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2059 2066 2162 2585 4064 1239
Size: 820 7 96 423 1479 2579
Fragments arranged by size:
2579 1479 820 423 96 7
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3529 4394 4692 3529
Size: 865 298 4241
Fragments arranged by size:
4241 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4067 882
Size: 617 2568 2219
Fragments arranged by size:
2568 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3195 493
Size: 711 1991 2702
Fragments arranged by size:
2702 1991 711
BsaBI GATnn'nnATC
Cuts at: 5398 5398
Size: 5404
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2388 3211 3593 4228
Size: 53 83 186 936 855 823 382 635
Cuts at: 4228 275
Size: 1451
Fragments arranged by size:
1451 936 855 823 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3837 4235 4509 4656 5067 3837
Size: 398 274 147 411 4174
Fragments arranged by size:
4174 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5179
Fragments arranged by size:
5179 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2011 2065 2318 1186
Size: 825 54 253 4272
Fragments arranged by size:
4272 825 253 54
BseYI C'CCAG_C
Cuts at: 2108 2501 4607 5103 2108
Size: 393 2106 496 2409
Fragments arranged by size:
2409 2106 496 393
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4831
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2159 2854 3615 4643 1540
Size: 275 186 158 695 761 1028 2301
Fragments arranged by size:
2301 1028 761 695 275 186 158
BsiHKAI G_wGCw'C
Cuts at: 2186 2384 2576 3465 3550 4621 2186
Size: 198 192 889 85 1071 2969
Fragments arranged by size:
2969 1071 889 198 192 85
BsmI GAATG_Cn'
Cuts at: 2915 3008 2915
Size: 93 5311
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2117 2186 2384 2576 2683 2723 3465
Size: 399 327 69 198 192 107 40 742
Cuts at: 3465 3550 4621 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 327 198 192 107
85 69 40
BspEI T'CCGG_A
Cuts at: 5067 5067
Size: 5404
BspHI T'CATG_A
Cuts at: 3293 5023 3293
Size: 1730 3674
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2340 2529 2643 2759 844
Size: 1496 189 114 116 3489
Fragments arranged by size:
3489 1496 189 116 114
BsrBI CCG'CTC
Cuts at: 1394 1823 2098 2189 2443 3291 1394
Size: 429 275 91 254 848 3507
Fragments arranged by size:
3507 848 429 275 254 91
BsrDI GCAATG_nn'
Cuts at: 65 1248 3899 4073 65
Size: 1183 2651 174 1396
Fragments arranged by size:
2651 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2358 4048 4235 1700
Size: 110 548 1690 187 2869
Fragments arranged by size:
2869 1690 548 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5404
BssHII G'CGCG_C
Cuts at: 1925 2256 1925
Size: 331 5073
BssSI C'ACGA_G
Cuts at: 3464 4476 3464
Size: 1012 4392
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5404
BstBI TT'CG_AA
Cuts at: 4208 4208
Size: 5404
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5404
BstXI CCAn_nnnn'nTGG
Cuts at: 2251 2251
Size: 5404
BstYI r'GATC_y
Cuts at: 1972 2238 2734 3101 3486 3503 4955 5041 5053
Size: 266 496 367 385 17 1452 86 12
Cuts at: 5053 5399 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 496 385 367 346 266 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2061 2118 513
Size: 552 939 57 57 3799
Fragments arranged by size:
3799 939 552 57 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4665
Fragments arranged by size:
4665 683 56
BtsI GCAGTG_nn'
Cuts at: 2267 2545 2920 3714 3734 2267
Size: 278 375 794 20 3937
Fragments arranged by size:
3937 794 375 278 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5404
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5369
DraI TTT'AAA
Cuts at: 2708 2708
Size: 5404
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4411 809
Size: 507 3095 1802
Fragments arranged by size:
3095 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2156 2427 2851
Size: 54 1425 50 275 18 326 271 424
Cuts at: 2851 3740 5369 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 424 326 275 271 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2156 2851 1537
Size: 275 344 695 4090
Fragments arranged by size:
4090 695 344 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2410 2545 3334 1353
Size: 1057 135 789 3423
Fragments arranged by size:
3423 1057 789 135
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3991 4365 4511 387
Size: 1064 138 2402 374 146 1280
Fragments arranged by size:
2402 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2184 2382 2184
Size: 198 5206
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2059 2066 2162 2585 3467 4064 4851
Size: 393 427 7 96 423 882 597 787
Cuts at: 4851 5119 1239
Size: 268 1524
Fragments arranged by size:
1524 882 787 597 427 423 393 268
96 7
EcoNI CCTnn'n_nnAGG
Cuts at: 2144 2144
Size: 5404
EcoO109I rG'GnC_Cy
Cuts at: 2087 2087
Size: 5404
EcoRI G'AATT_C
Cuts at: 2713 3091 2713
Size: 378 5026
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2311 2311
Size: 5404
FspI TGC'GCA
Cuts at: 3910 3910
Size: 5404
HaeII r_GCGC'y
Cuts at: 1678 2012 2109 2197 2251 2391 4551 1678
Size: 334 97 88 54 140 2160 2531
Fragments arranged by size:
2531 2160 334 140 97 88 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4045 4077 4119 4151 1373
Size: 32 135 32 2473 32 42 32 2626
Fragments arranged by size:
2626 2473 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2748 2994 669
Size: 988 281 810 246 3079
Fragments arranged by size:
3079 988 810 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5404
HpaI GTT'AAC
Cuts at: 2994 2994
Size: 5404
KasI G'GCGC_C
Cuts at: 2387 2387
Size: 5404
KpnI G_GTAC'C
Cuts at: 2729 2729
Size: 5404
MfeI C'AATT_G
Cuts at: 3003 3003
Size: 5404
MluI A'CGCG_T
Cuts at: 2166 5280 2166
Size: 3114 2290
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4518 4702 1305
Size: 3213 184 2007
Fragments arranged by size:
3213 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2429 10
Size: 54 1425 343 597 2985
Fragments arranged by size:
2985 1425 597 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3362 3721 3880 518
Size: 648 2196 359 159 2042
Fragments arranged by size:
2196 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2120 2403 2433 2820 3497 4645 4890 1606
Size: 514 283 30 387 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 514 387 283 245 30
NarI GG'CG_CC
Cuts at: 2388 2388
Size: 5404
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3913
Fragments arranged by size:
3913 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5404
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5404
NotI GC'GGCC_GC
Cuts at: 2851 2851
Size: 5404
NspI r_CATG'y
Cuts at: 1132 1229 2762 4307 1132
Size: 97 1533 1545 2229
Fragments arranged by size:
2229 1545 1533 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5404
PciI A'CATG_T
Cuts at: 4303 4303
Size: 5404
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5404
PmeI GTTT'AAAC
Cuts at: 2708 2708
Size: 5404
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3473 3505 4986 5018 3473
Size: 32 1481 32 3859
Fragments arranged by size:
3859 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2087 2087
Size: 5404
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5404
PsiI TTA'TAA
Cuts at: 2974 2974
Size: 5404
PstI C_TGCA'G
Cuts at: 830 2756 830
Size: 1926 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5404
SacI G_AGCT'C
Cuts at: 2186 2384 2186
Size: 198 5206
SacII CC_GC'GG
Cuts at: 2121 2121
Size: 5404
SalI G'TCGA_C
Cuts at: 1936 2746 1936
Size: 810 4594
SanDI GG'GwC_CC
Cuts at: 2087 2087
Size: 5404
SapI GCTCTTCn'nnn_
Cuts at: 2545 2545
Size: 5404
SbfI CC_TGCA'GG
Cuts at: 2756 2756
Size: 5404
ScaI AGT'ACT
Cuts at: 3652 5364 3652
Size: 1712 3692
SfcI C'TryA_G
Cuts at: 826 1045 2752 3887 4568 4759 826
Size: 219 1707 1135 681 191 1471
Fragments arranged by size:
1707 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2389 2389
Size: 5404
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5404
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5404
SmaI CCC'GGG
Cuts at: 1872 2128 2731 1872
Size: 256 603 4545
Fragments arranged by size:
4545 603 256
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3508 4409 4671 820
Size: 197 926 1565 901 262 1553
Fragments arranged by size:
1565 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3195 493
Size: 2702 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5404
SphI G_CATG'C
Cuts at: 1132 2762 1132
Size: 1630 3774
SspI AAT'ATT
Cuts at: 5 52 3328 5
Size: 47 3276 2081
Fragments arranged by size:
3276 2081 47
StuI AGG'CCT
Cuts at: 1517 2036 1517
Size: 519 4885
StyI C'CwwG_G
Cuts at: 513 1065 2004 2594 2843 513
Size: 552 939 590 249 3074
Fragments arranged by size:
3074 939 590 552 249
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2483 3431 3448 3601 3786 4231 325
Size: 1255 903 948 17 153 185 445 1498
Fragments arranged by size:
1498 1255 948 903 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3650 5362 96
Size: 274 80 33 51 1443 1673 1712 138
Fragments arranged by size:
1712 1673 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5364 5396 5364
Size: 32 5372
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5404
XbaI T'CTAG_A
Cuts at: 2740 2740
Size: 5404
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5404
XmaI C'CCGG_G
Cuts at: 1870 2126 2729 1870
Size: 256 603 4545
Fragments arranged by size:
4545 603 256
XmnI GAAnn'nnTTC
Cuts at: 2168 3533 2168
Size: 1365 4039
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3212 276
Size: 53 83 186 936 1678 2468
Fragments arranged by size:
2468 1678 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI
NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI PvuII RsrII SexAI SfiI SrfI SwaI
XcmI