Restriction map for
pFN21AB8894
(Circular) (Six-base) MAPSORT of: pFN21AB8894.seq Check: 6425 from: 1 to: 5356
pFN21AB8894 5356 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3166 278
Size: 53 83 186 936 1630 2468
Fragments arranged by size:
2468 1630 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2699 1937
Size: 762 4594
Acc65I G'GTAC_C
Cuts at: 2677 2677
Size: 5356
AclI AA'CG_TT
Cuts at: 1563 3483 3856 1563
Size: 1920 373 3063
Fragments arranged by size:
3063 1920 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3419 4803 5071 1632
Size: 1787 1384 268 1917
Fragments arranged by size:
1917 1787 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5159
AflIII A'CryG_T
Cuts at: 1203 4255 5232 1203
Size: 3052 977 1327
Fragments arranged by size:
3052 1327 977
AgeI A'CCGG_T
Cuts at: 4187 4187
Size: 5356
AhdI GACnn_n'nnGTC
Cuts at: 4085 4085
Size: 5356
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5356
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5356
AlwNI CAG_nnn'CTG
Cuts at: 2106 3099 4671 2106
Size: 993 1572 2791
Fragments arranged by size:
2791 1572 993
ApaI G_GGCC'C
Cuts at: 2271 2641 2271
Size: 370 4986
ApaLI G'TGCA_C
Cuts at: 2042 3413 4569 2042
Size: 1371 1156 2829
Fragments arranged by size:
2829 1371 1156
ApoI r'AATT_y
Cuts at: 1493 2665 2891 3043 5152 1493
Size: 1172 226 152 2109 1697
Fragments arranged by size:
2109 1697 1172 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3910 160
Size: 872 2878 1606
Fragments arranged by size:
2878 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5356
AvaI C'yCGr_G
Cuts at: 1870 1943 2156 2201 2272 2681 1870
Size: 73 213 45 71 409 4545
Fragments arranged by size:
4545 409 213 73 71 45
BamHI G'GATC_C
Cuts at: 2686 3053 2686
Size: 367 4989
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2216 2251 2261 2309 2677
Size: 325 217 627 429 35 10 48 368
Cuts at: 2677 4132 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 429 368 325 217 48
35 10
BanII G_rGCy'C
Cuts at: 1391 2271 2641 2649 2675 1391
Size: 880 370 8 26 4072
Fragments arranged by size:
4072 880 370 26 8
BbeI G_GCGC'C
Cuts at: 2220 2265 2220
Size: 45 5311
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2528 928
Size: 971 629 3756
Fragments arranged by size:
3756 971 629
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3547 3581 1903
Size: 34 1610 34 3678
Fragments arranged by size:
3678 1610 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3248 4458 3248
Size: 1210 4146
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5356
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2507 2616 3967
Size: 107 122 71 631 605 835 109 1351
Cuts at: 3967 136
Size: 1525
Fragments arranged by size:
1525 1351 835 631 605 122 109 107
71
BglII A'GATC_T
Cuts at: 5351 5351
Size: 5356
BlpI GC'TnA_GC
Cuts at: 2773 2773
Size: 5356
Bme1580I G_kGCm'C
Cuts at: 1790 2046 2271 2641 3417 4573 1790
Size: 256 225 370 776 1156 2573
Fragments arranged by size:
2573 1156 776 370 256 225
BmgBI CAC'GTC
Cuts at: 1318 2530 2632 1318
Size: 1212 102 4042
Fragments arranged by size:
4042 1212 102
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3087 4045 442
Size: 416 53 83 399 24 1670 958 1753
Fragments arranged by size:
1753 1670 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5356
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2663 4016 1239
Size: 1424 1353 2579
Fragments arranged by size:
2579 1424 1353
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2633 3481 4346 4644 2633
Size: 848 865 298 3345
Fragments arranged by size:
3345 865 848 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4019 882
Size: 617 2520 2219
Fragments arranged by size:
2520 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3147 493
Size: 711 1943 2702
Fragments arranged by size:
2702 1943 711
BsaBI GATnn'nnATC
Cuts at: 5350 5350
Size: 5356
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2217 2262 3163 3545
Size: 53 83 186 936 684 45 901 382
Cuts at: 3545 4180 275
Size: 635 1451
Fragments arranged by size:
1451 936 901 684 635 382 186 83
53 45
BsaWI w'CCGG_w
Cuts at: 3789 4187 4461 4608 5019 3789
Size: 398 274 147 411 4126
Fragments arranged by size:
4126 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5131
Fragments arranged by size:
5131 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2132 2552 1186
Size: 946 420 3990
Fragments arranged by size:
3990 946 420
BseYI C'CCAG_C
Cuts at: 4559 5055 4559
Size: 496 4860
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4783
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2806 3567 4595 1540
Size: 275 186 805 761 1028 2301
Fragments arranged by size:
2301 1028 805 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2046 2649 3417 3502 4573 2046
Size: 603 768 85 1071 2829
Fragments arranged by size:
2829 1071 768 603 85
BsmI GAATG_Cn'
Cuts at: 2867 2960 2867
Size: 93 5263
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2046 2271 2641 2649 2675 3417 3502
Size: 399 256 225 370 8 26 742 85
Cuts at: 3502 4573 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 370 256 225 85
26 8
BspEI T'CCGG_A
Cuts at: 5019 5019
Size: 5356
BspHI T'CATG_A
Cuts at: 3245 4975 3245
Size: 1730 3626
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2472 2644 2711 844
Size: 1628 172 67 3489
Fragments arranged by size:
3489 1628 172 67
BsrBI CCG'CTC
Cuts at: 1394 1823 2329 3243 1394
Size: 429 506 914 3507
Fragments arranged by size:
3507 914 506 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3851 4025 65
Size: 1183 2603 174 1396
Fragments arranged by size:
2603 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2222 4000 4187 1700
Size: 110 412 1778 187 2869
Fragments arranged by size:
2869 1778 412 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5356
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5356
BssSI C'ACGA_G
Cuts at: 2048 3416 4428 2048
Size: 1368 1012 2976
Fragments arranged by size:
2976 1368 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5356
BstBI TT'CG_AA
Cuts at: 4160 4160
Size: 5356
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5356
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5356
BstYI r'GATC_y
Cuts at: 1972 2686 3053 3438 3455 4907 4993 5005 5351
Size: 714 367 385 17 1452 86 12 346
Cuts at: 5351 1972
Size: 1977
Fragments arranged by size:
1977 1452 714 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2134 513
Size: 552 1069 3735
Fragments arranged by size:
3735 1069 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2431 487
Size: 683 56 1205 3412
Fragments arranged by size:
3412 1205 683 56
BtsI GCAGTG_nn'
Cuts at: 2872 3666 3686 2872
Size: 794 20 4542
Fragments arranged by size:
4542 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5356
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5321
DraI TTT'AAA
Cuts at: 2660 2660
Size: 5356
DraIII CAC_nnn'GTG
Cuts at: 2038 2038
Size: 5356
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4363 809
Size: 507 3047 1802
Fragments arranged by size:
3047 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2149 2433 2469
Size: 54 1425 50 275 18 319 284 36
Cuts at: 2469 2621 2803 3692 5321 8
Size: 152 182 889 1629 43
Fragments arranged by size:
1629 1425 889 319 284 275 182 152
54 50 43 36 18
EagI C'GGCC_G
Cuts at: 1537 1812 2803 1537
Size: 275 991 4090
Fragments arranged by size:
4090 991 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2537 3286 1353
Size: 1184 749 3423
Fragments arranged by size:
3423 1184 749
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2510 3943 4317 4463 387
Size: 1064 138 921 1433 374 146 1280
Fragments arranged by size:
1433 1280 1064 921 374 146 138
EcoICRI GAG'CTC
Cuts at: 2647 2647
Size: 5356
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2663 3419 4016 4803 5071 1239
Size: 393 1031 756 597 787 268 1524
Fragments arranged by size:
1524 1031 787 756 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2268 2638 2268
Size: 370 4986
EcoRI G'AATT_C
Cuts at: 2665 3043 2665
Size: 378 4978
FspI TGC'GCA
Cuts at: 3862 3862
Size: 5356
HaeII r_GCGC'y
Cuts at: 1678 2220 2265 2305 2358 4503 1678
Size: 542 45 40 53 2145 2531
Fragments arranged by size:
2531 2145 542 53 45 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3997 4029 4071 4103 1373
Size: 32 135 32 2425 32 42 32 2626
Fragments arranged by size:
2626 2425 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2700 2946 669
Size: 988 281 762 246 3079
Fragments arranged by size:
3079 988 762 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5356
HpaI GTT'AAC
Cuts at: 2946 2946
Size: 5356
KasI G'GCGC_C
Cuts at: 2216 2261 2216
Size: 45 5311
KpnI G_GTAC'C
Cuts at: 2681 2681
Size: 5356
MfeI C'AATT_G
Cuts at: 2955 2955
Size: 5356
MluI A'CGCG_T
Cuts at: 5232 5232
Size: 5356
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4470 4654 1305
Size: 3165 184 2007
Fragments arranged by size:
3165 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2435 2471 10
Size: 54 1425 343 603 36 2895
Fragments arranged by size:
2895 1425 603 343 54 36
MslI CAynn'nnrTG
Cuts at: 518 1166 3314 3673 3832 518
Size: 648 2148 359 159 2042
Fragments arranged by size:
2148 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2084 2106 2214 2772 3449 4597 4842 1606
Size: 478 22 108 558 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 558 478 245 108 22
NaeI GCC'GGC
Cuts at: 2224 2224
Size: 5356
NarI GG'CG_CC
Cuts at: 2217 2262 2217
Size: 45 5311
NcoI C'CATG_G
Cuts at: 513 1065 2134 513
Size: 552 1069 3735
Fragments arranged by size:
3735 1069 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5356
NgoMIV G'CCGG_C
Cuts at: 2222 2222
Size: 5356
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5356
NotI GC'GGCC_GC
Cuts at: 2803 2803
Size: 5356
NspI r_CATG'y
Cuts at: 1132 1229 2714 4259 1132
Size: 97 1485 1545 2229
Fragments arranged by size:
2229 1545 1485 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5356
PciI A'CATG_T
Cuts at: 4255 4255
Size: 5356
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2038 1108
Size: 930 4426
PmeI GTTT'AAAC
Cuts at: 2660 2660
Size: 5356
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3425 3457 4938 4970 3425
Size: 32 1481 32 3811
Fragments arranged by size:
3811 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5356
PsiI TTA'TAA
Cuts at: 2926 2926
Size: 5356
PspOMI G'GGCC_C
Cuts at: 2267 2637 2267
Size: 370 4986
PstI C_TGCA'G
Cuts at: 830 2111 2708 830
Size: 1281 597 3478
Fragments arranged by size:
3478 1281 597
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5356
PvuII CAG'CTG
Cuts at: 2084 2084
Size: 5356
SacI G_AGCT'C
Cuts at: 2649 2649
Size: 5356
SalI G'TCGA_C
Cuts at: 1936 2698 1936
Size: 762 4594
SbfI CC_TGCA'GG
Cuts at: 2708 2708
Size: 5356
ScaI AGT'ACT
Cuts at: 3604 5316 3604
Size: 1712 3644
SfcI C'TryA_G
Cuts at: 826 1045 2107 2176 2194 2704 3839 4520 4711
Size: 219 1062 69 18 510 1135 681 191
Cuts at: 4711 826
Size: 1471
Fragments arranged by size:
1471 1135 1062 681 510 219 191 69
18
SfoI GGC'GCC
Cuts at: 2218 2263 2218
Size: 45 5311
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5356
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5356
SmaI CCC'GGG
Cuts at: 1872 2274 2683 1872
Size: 402 409 4545
Fragments arranged by size:
4545 409 402
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2648 3460 4361 4623 820
Size: 197 926 705 812 901 262 1553
Fragments arranged by size:
1553 926 901 812 705 262 197
SnaBI TAC'GTA
Cuts at: 493 3147 493
Size: 2654 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5356
SphI G_CATG'C
Cuts at: 1132 2714 1132
Size: 1582 3774
SspI AAT'ATT
Cuts at: 5 52 3280 5
Size: 47 3228 2081
Fragments arranged by size:
3228 2081 47
StuI AGG'CCT
Cuts at: 1517 2501 1517
Size: 984 4372
StyI C'CwwG_G
Cuts at: 513 1065 2134 2472 2795 513
Size: 552 1069 338 323 3074
Fragments arranged by size:
3074 1069 552 338 323
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2540 3383 3400 3553 3738 4183 325
Size: 1255 960 843 17 153 185 445 1498
Fragments arranged by size:
1498 1255 960 843 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3602 5314 96
Size: 274 80 33 51 1443 1625 1712 138
Fragments arranged by size:
1712 1625 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5316 5348 5316
Size: 32 5324
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5356
XbaI T'CTAG_A
Cuts at: 2692 2692
Size: 5356
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5356
XmaI C'CCGG_G
Cuts at: 1870 2272 2681 1870
Size: 402 409 4545
Fragments arranged by size:
4545 409 402
XmnI GAAnn'nnTTC
Cuts at: 3485 3485
Size: 5356
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3164 276
Size: 53 83 186 936 1630 2468
Fragments arranged by size:
2468 1630 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV
FalI FseI FspAI NruI NsiI PacI PfoI PmlI
PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI
SfiI SrfI SwaI XcmI