Restriction map for
pFN21AB8893
(Circular) (Six-base) MAPSORT of: pFN21AB8893.seq Check: 8549 from: 1 to: 5356
pFN21AB8893 5356 bp
With 182 enzymes: SgfI *
January 30, 2014 17:16 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3166 278
Size: 53 83 186 936 1630 2468
Fragments arranged by size:
2468 1630 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2223 2699 1937
Size: 286 476 4594
Fragments arranged by size:
4594 476 286
Acc65I G'GTAC_C
Cuts at: 2677 2677
Size: 5356
AclI AA'CG_TT
Cuts at: 1563 3483 3856 1563
Size: 1920 373 3063
Fragments arranged by size:
3063 1920 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2652 3419 4803 5071 1632
Size: 1020 767 1384 268 1917
Fragments arranged by size:
1917 1384 1020 767 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5159
AflIII A'CryG_T
Cuts at: 1203 4255 5232 1203
Size: 3052 977 1327
Fragments arranged by size:
3052 1327 977
AgeI A'CCGG_T
Cuts at: 4187 4187
Size: 5356
AhdI GACnn_n'nnGTC
Cuts at: 4085 4085
Size: 5356
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5356
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5356
AlwNI CAG_nnn'CTG
Cuts at: 2408 2452 3099 4671 2408
Size: 44 647 1572 3093
Fragments arranged by size:
3093 1572 647 44
ApaI G_GGCC'C
Cuts at: 2606 2606
Size: 5356
ApaLI G'TGCA_C
Cuts at: 3413 4569 3413
Size: 1156 4200
ApoI r'AATT_y
Cuts at: 1493 2468 2665 2891 3043 5152 1493
Size: 975 197 226 152 2109 1697
Fragments arranged by size:
2109 1697 975 226 197 152
AseI AT'TA_AT
Cuts at: 160 1032 3910 160
Size: 872 2878 1606
Fragments arranged by size:
2878 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5356
AvaI C'yCGr_G
Cuts at: 1870 1943 2125 2243 2605 2681 1870
Size: 73 182 118 362 76 4545
Fragments arranged by size:
4545 362 182 118 76 73
BamHI G'GATC_C
Cuts at: 2686 3053 2686
Size: 367 4989
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2330 2677 4132 618
Size: 325 217 627 543 347 1455 1842
Fragments arranged by size:
1842 1455 627 543 347 325 217
BanII G_rGCy'C
Cuts at: 1391 2244 2606 2675 1391
Size: 853 362 69 4072
Fragments arranged by size:
4072 853 362 69
BbeI G_GCGC'C
Cuts at: 2334 2334
Size: 5356
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4385
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2494 2528 3547 3581 1903
Size: 34 557 34 1019 34 3678
Fragments arranged by size:
3678 1019 557 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3248 4458 3248
Size: 1210 4146
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5356
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2324 3967 136
Size: 107 122 71 631 605 652 1643 1525
Fragments arranged by size:
1643 1525 652 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5351 5351
Size: 5356
BlpI GC'TnA_GC
Cuts at: 2773 2773
Size: 5356
Bme1580I G_kGCm'C
Cuts at: 1790 2606 3417 4573 1790
Size: 816 811 1156 2573
Fragments arranged by size:
2573 1156 816 811
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5356
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3087 4045 442
Size: 416 53 83 399 24 1670 958 1753
Fragments arranged by size:
1753 1670 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5356
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2474 2480 4016 1239
Size: 1235 6 1536 2579
Fragments arranged by size:
2579 1536 1235 6
Bpu10I CC'TnA_GC
Cuts at: 2401 2401
Size: 5356
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2038 3481 4346 4644 2038
Size: 1443 865 298 2750
Fragments arranged by size:
2750 1443 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4019 882
Size: 617 2520 2219
Fragments arranged by size:
2520 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3147 493
Size: 711 1943 2702
Fragments arranged by size:
2702 1943 711
BsaBI GATnn'nnATC
Cuts at: 5350 5350
Size: 5356
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2220 2331 3163 3545
Size: 53 83 186 936 687 111 832 382
Cuts at: 3545 4180 275
Size: 635 1451
Fragments arranged by size:
1451 936 832 687 635 382 186 111
83 53
BsaWI w'CCGG_w
Cuts at: 2072 2376 3789 4187 4461 4608 5019 2072
Size: 304 1413 398 274 147 411 2409
Fragments arranged by size:
2409 1413 411 398 304 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5131
Fragments arranged by size:
5131 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2654 1186
Size: 1468 3888
BseYI C'CCAG_C
Cuts at: 2118 4559 5055 2118
Size: 2441 496 2419
Fragments arranged by size:
2441 2419 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4783
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2217 2232 2364 2806 3567 4595
Size: 275 186 216 15 132 442 761 1028
Cuts at: 4595 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 442 275 216 186 132
15
BsiHKAI G_wGCw'C
Cuts at: 3417 3502 4573 3417
Size: 85 1071 4200
Fragments arranged by size:
4200 1071 85
BsmI GAATG_Cn'
Cuts at: 2032 2867 2960 2032
Size: 835 93 4428
Fragments arranged by size:
4428 835 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2244 2606 2675 3417 3502 4573 1391
Size: 399 454 362 69 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 454 399 362 85 69
BspEI T'CCGG_A
Cuts at: 2376 5019 2376
Size: 2643 2713
BspHI T'CATG_A
Cuts at: 3245 4975 3245
Size: 1730 3626
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2711 844
Size: 1867 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2138 3243 1394
Size: 429 315 1105 3507
Fragments arranged by size:
3507 1105 429 315
BsrDI GCAATG_nn'
Cuts at: 65 1248 3851 4025 65
Size: 1183 2603 174 1396
Fragments arranged by size:
2603 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2216 2231 2565 2615 4000 4187 1700
Size: 110 406 15 334 50 1385 187 2869
Fragments arranged by size:
2869 1385 406 334 187 110 50 15
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5356
BssHII G'CGCG_C
Cuts at: 1925 2271 1925
Size: 346 5010
BssSI C'ACGA_G
Cuts at: 3416 4428 3416
Size: 1012 4344
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5356
BstBI TT'CG_AA
Cuts at: 4160 4160
Size: 5356
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5356
BstYI r'GATC_y
Cuts at: 1972 2379 2686 3053 3438 3455 4907 4993 5005
Size: 407 307 367 385 17 1452 86 12
Cuts at: 5005 5351 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 407 385 367 346 307 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2598 513
Size: 552 1533 3271
Fragments arranged by size:
3271 1533 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4617
Fragments arranged by size:
4617 683 56
BtsI GCAGTG_nn'
Cuts at: 2266 2872 3666 3686 2266
Size: 606 794 20 3936
Fragments arranged by size:
3936 794 606 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5356
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5321
DraI TTT'AAA
Cuts at: 2660 2660
Size: 5356
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4363 809
Size: 507 3047 1802
Fragments arranged by size:
3047 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2214 2229 2361
Size: 54 1425 50 275 18 384 15 132
Cuts at: 2361 2617 2803 3692 5321 8
Size: 256 186 889 1629 43
Fragments arranged by size:
1629 1425 889 384 275 256 186 132
54 50 43 18 15
EagI C'GGCC_G
Cuts at: 1537 1812 2214 2229 2361 2803 1537
Size: 275 402 15 132 442 4090
Fragments arranged by size:
4090 442 402 275 132 15
EarI CTCTTCn'nnn_
Cuts at: 1353 2640 2646 3286 1353
Size: 1287 6 640 3423
Fragments arranged by size:
3423 1287 640 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2305 3943 4317 4463 387
Size: 1064 138 716 1638 374 146 1280
Fragments arranged by size:
1638 1280 1064 716 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2474 2480 2652 3419 4016 4803 5071
Size: 393 842 6 172 767 597 787 268
Cuts at: 5071 1239
Size: 1524
Fragments arranged by size:
1524 842 787 767 597 393 268 172
6
EcoRI G'AATT_C
Cuts at: 2665 3043 2665
Size: 378 4978
FspI TGC'GCA
Cuts at: 3862 3862
Size: 5356
HaeII r_GCGC'y
Cuts at: 1678 2125 2151 2334 4503 1678
Size: 447 26 183 2169 2531
Fragments arranged by size:
2531 2169 447 183 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3997 4029 4071 4103 1373
Size: 32 135 32 2425 32 42 32 2626
Fragments arranged by size:
2626 2425 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2700 2946 669
Size: 988 281 762 246 3079
Fragments arranged by size:
3079 988 762 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5356
HpaI GTT'AAC
Cuts at: 2946 2946
Size: 5356
KasI G'GCGC_C
Cuts at: 2330 2330
Size: 5356
KpnI G_GTAC'C
Cuts at: 2681 2681
Size: 5356
MfeI C'AATT_G
Cuts at: 2955 2955
Size: 5356
MluI A'CGCG_T
Cuts at: 5232 5232
Size: 5356
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2136 4470 4654 1305
Size: 831 2334 184 2007
Fragments arranged by size:
2334 2007 831 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3534
Fragments arranged by size:
3534 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3314 3673 3832 518
Size: 648 2148 359 159 2042
Fragments arranged by size:
2148 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2600 2772 3449 4597 4842 1606
Size: 994 172 677 1148 245 2120
Fragments arranged by size:
2120 1148 994 677 245 172
NaeI GCC'GGC
Cuts at: 2218 2233 2218
Size: 15 5341
NarI GG'CG_CC
Cuts at: 2331 2331
Size: 5356
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4804
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5356
NgoMIV G'CCGG_C
Cuts at: 2216 2231 2216
Size: 15 5341
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5356
NotI GC'GGCC_GC
Cuts at: 2803 2803
Size: 5356
NspI r_CATG'y
Cuts at: 1132 1229 2266 2714 4259 1132
Size: 97 1037 448 1545 2229
Fragments arranged by size:
2229 1545 1037 448 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5356
PciI A'CATG_T
Cuts at: 4255 4255
Size: 5356
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2452 1108
Size: 1344 4012
PmeI GTTT'AAAC
Cuts at: 2660 2660
Size: 5356
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3425 3457 4938 4970 3425
Size: 32 1481 32 3811
Fragments arranged by size:
3811 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5356
PsiI TTA'TAA
Cuts at: 2926 2926
Size: 5356
PspOMI G'GGCC_C
Cuts at: 2602 2602
Size: 5356
PspXI vC'TCGA_Gb
Cuts at: 2243 2243
Size: 5356
PstI C_TGCA'G
Cuts at: 830 2708 830
Size: 1878 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5356
SacII CC_GC'GG
Cuts at: 2601 2601
Size: 5356
SalI G'TCGA_C
Cuts at: 1936 2698 1936
Size: 762 4594
SbfI CC_TGCA'GG
Cuts at: 2708 2708
Size: 5356
ScaI AGT'ACT
Cuts at: 3604 5316 3604
Size: 1712 3644
SfcI C'TryA_G
Cuts at: 826 1045 2704 3839 4520 4711 826
Size: 219 1659 1135 681 191 1471
Fragments arranged by size:
1659 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2332 2332
Size: 5356
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5356
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5356
SmaI CCC'GGG
Cuts at: 1872 2127 2607 2683 1872
Size: 255 480 76 4545
Fragments arranged by size:
4545 480 255 76
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2053 2243 3460 4361 4623 820
Size: 197 926 110 190 1217 901 262 1553
Fragments arranged by size:
1553 1217 926 901 262 197 190 110
SnaBI TAC'GTA
Cuts at: 493 3147 493
Size: 2654 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5356
SphI G_CATG'C
Cuts at: 1132 2714 1132
Size: 1582 3774
SrfI GCCC'GGGC
Cuts at: 2127 2127
Size: 5356
SspI AAT'ATT
Cuts at: 5 52 3280 5
Size: 47 3228 2081
Fragments arranged by size:
3228 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5356
StyI C'CwwG_G
Cuts at: 513 1065 2319 2334 2795 513
Size: 552 1254 15 461 3074
Fragments arranged by size:
3074 1254 552 461 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3383 3400 3553 3738 4183 325
Size: 1255 1803 17 153 185 445 1498
Fragments arranged by size:
1803 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3602 5314 96
Size: 274 80 33 51 1443 1625 1712 138
Fragments arranged by size:
1712 1625 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5316 5348 5316
Size: 32 5324
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5356
XbaI T'CTAG_A
Cuts at: 2692 2692
Size: 5356
XhoI C'TCGA_G
Cuts at: 1943 2243 1943
Size: 300 5056
XmaI C'CCGG_G
Cuts at: 1870 2125 2605 2681 1870
Size: 255 480 76 4545
Fragments arranged by size:
4545 480 255 76
XmnI GAAnn'nnTTC
Cuts at: 3485 3485
Size: 5356
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3164 276
Size: 53 83 186 936 1630 2468
Fragments arranged by size:
2468 1630 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PspXI PstI PvuI SacII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI NruI NsiI
PacI PfoI PmlI PpuMI PsrI PvuII RsrII SacI
SanDI SapI SexAI SfiI SwaI XcmI