Restriction map for
pFN21AB8888
(Circular) (Six-base) MAPSORT of: pFN21AB8888.seq Check: 9328 from: 1 to: 5308
pFN21AB8888 5308 bp
With 182 enzymes: SgfI *
December 8, 2014 13:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3118 278
Size: 53 83 186 936 1582 2468
Fragments arranged by size:
2468 1582 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2651 1937
Size: 714 4594
Acc65I G'GTAC_C
Cuts at: 2629 2629
Size: 5308
AclI AA'CG_TT
Cuts at: 1563 3435 3808 1563
Size: 1872 373 3063
Fragments arranged by size:
3063 1872 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3371 4755 5023 1632
Size: 1739 1384 268 1917
Fragments arranged by size:
1917 1739 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5111
AflIII A'CryG_T
Cuts at: 1203 4207 5184 1203
Size: 3004 977 1327
Fragments arranged by size:
3004 1327 977
AgeI A'CCGG_T
Cuts at: 4139 4139
Size: 5308
AhdI GACnn_n'nnGTC
Cuts at: 4037 4037
Size: 5308
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5308
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2411 1184
Size: 1227 4081
AlwNI CAG_nnn'CTG
Cuts at: 3051 4623 3051
Size: 1572 3736
ApaI G_GGCC'C
Cuts at: 2445 2445
Size: 5308
ApaLI G'TGCA_C
Cuts at: 2129 3365 4521 2129
Size: 1236 1156 2916
Fragments arranged by size:
2916 1236 1156
ApoI r'AATT_y
Cuts at: 1493 2617 2843 2995 5104 1493
Size: 1124 226 152 2109 1697
Fragments arranged by size:
2109 1697 1124 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3862 160
Size: 872 2830 1606
Fragments arranged by size:
2830 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5308
AvaI C'yCGr_G
Cuts at: 1870 1943 2054 2101 2633 1870
Size: 73 111 47 532 4545
Fragments arranged by size:
4545 532 111 73 47
BamHI G'GATC_C
Cuts at: 2638 3005 2638
Size: 367 4941
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2029 2156 2629 4084 618
Size: 325 217 627 242 127 473 1455 1842
Fragments arranged by size:
1842 1455 627 473 325 242 217 127
BanII G_rGCy'C
Cuts at: 1391 2066 2108 2226 2322 2445 2627 1391
Size: 675 42 118 96 123 182 4072
Fragments arranged by size:
4072 675 182 123 118 96 42
BbeI G_GCGC'C
Cuts at: 2033 2160 2033
Size: 127 5181
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4337
BbvCI CC'TCA_GC
Cuts at: 2552 2552
Size: 5308
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3499 3533 1903
Size: 34 1562 34 3678
Fragments arranged by size:
3678 1562 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3200 4410 3200
Size: 1210 4098
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5308
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2154 2214 2291
Size: 107 122 71 631 605 482 60 77
Cuts at: 2291 3919 136
Size: 1628 1525
Fragments arranged by size:
1628 1525 631 605 482 122 107 77
71 60
BglII A'GATC_T
Cuts at: 2535 5303 2535
Size: 2768 2540
BlpI GC'TnA_GC
Cuts at: 2725 2725
Size: 5308
Bme1580I G_kGCm'C
Cuts at: 1790 2133 2445 3369 4525 1790
Size: 343 312 924 1156 2573
Fragments arranged by size:
2573 1156 924 343 312
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5308
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3039 3997 442
Size: 416 53 83 399 24 1622 958 1753
Fragments arranged by size:
1753 1622 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5308
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2135 2350 3968 1239
Size: 896 215 1618 2579
Fragments arranged by size:
2579 1618 896 215
Bpu10I CC'TnA_GC
Cuts at: 2552 2552
Size: 5308
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3433 4298 4596 3433
Size: 865 298 4145
Fragments arranged by size:
4145 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2253 3971 882
Size: 617 754 1718 2219
Fragments arranged by size:
2219 1718 754 617
BsaAI yAC'GTr
Cuts at: 493 1204 3099 493
Size: 711 1895 2702
Fragments arranged by size:
2702 1895 711
BsaBI GATnn'nnATC
Cuts at: 5302 5302
Size: 5308
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2030 2157 3115 3497
Size: 53 83 186 936 497 127 958 382
Cuts at: 3497 4132 275
Size: 635 1451
Fragments arranged by size:
1451 958 936 635 497 382 186 127
83 53
BsaWI w'CCGG_w
Cuts at: 3741 4139 4413 4560 4971 3741
Size: 398 274 147 411 4078
Fragments arranged by size:
4078 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5083
Fragments arranged by size:
5083 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5308
BseYI C'CCAG_C
Cuts at: 2523 4511 5007 2523
Size: 1988 496 2824
Fragments arranged by size:
2824 1988 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2524 1163
Size: 573 788 3947
Fragments arranged by size:
3947 788 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2329 2758 3519 4547 1540
Size: 275 186 328 429 761 1028 2301
Fragments arranged by size:
2301 1028 761 429 328 275 186
BsiHKAI G_wGCw'C
Cuts at: 2133 3369 3454 4525 2133
Size: 1236 85 1071 2916
Fragments arranged by size:
2916 1236 1071 85
BsmI GAATG_Cn'
Cuts at: 2819 2912 2819
Size: 93 5215
BsmBI CGTCTCn'nnnn_
Cuts at: 2342 2342
Size: 5308
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2066 2108 2133 2226 2322 2445 2627
Size: 399 276 42 25 93 96 123 182
Cuts at: 2627 3369 3454 4525 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 276 182 123 96
93 85 42 25
BspEI T'CCGG_A
Cuts at: 4971 4971
Size: 5308
BspHI T'CATG_A
Cuts at: 3197 4927 3197
Size: 1730 3578
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2663 844
Size: 1819 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3195 1394
Size: 429 1372 3507
Fragments arranged by size:
3507 1372 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3803 3977 65
Size: 1183 2555 174 1396
Fragments arranged by size:
2555 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2208 2468 3952 4139 1700
Size: 110 398 260 1484 187 2869
Fragments arranged by size:
2869 1484 398 260 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5308
BssHII G'CGCG_C
Cuts at: 1925 2138 2249 2462 2464 1925
Size: 213 111 213 2 4769
Fragments arranged by size:
4769 213 213 111 2
BssSI C'ACGA_G
Cuts at: 3368 4380 3368
Size: 1012 4296
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2547 794
Size: 1753 3555
BstBI TT'CG_AA
Cuts at: 4112 4112
Size: 5308
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5308
BstYI r'GATC_y
Cuts at: 1972 2535 2638 3005 3390 3407 4859 4945 4957
Size: 563 103 367 385 17 1452 86 12
Cuts at: 4957 5303 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 563 385 367 346 103 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2086 513
Size: 552 1021 3735
Fragments arranged by size:
3735 1021 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4569
Fragments arranged by size:
4569 683 56
BtsI GCAGTG_nn'
Cuts at: 2611 2824 3618 3638 2611
Size: 213 794 20 4281
Fragments arranged by size:
4281 794 213 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5308
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5273
DraI TTT'AAA
Cuts at: 2612 2612
Size: 5308
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4315 809
Size: 507 2999 1802
Fragments arranged by size:
2999 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2210 2755 3644
Size: 54 1425 50 275 18 380 545 889
Cuts at: 3644 5273 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 545 380 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2755 1537
Size: 275 943 4090
Fragments arranged by size:
4090 943 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3238 1353
Size: 1885 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2179 3895 4269 4415 387
Size: 1064 138 590 1716 374 146 1280
Fragments arranged by size:
1716 1280 1064 590 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2135 2350 3371 3968 4755 5023 1239
Size: 393 503 215 1021 597 787 268 1524
Fragments arranged by size:
1524 1021 787 597 503 393 268 215
EcoRI G'AATT_C
Cuts at: 2617 2995 2617
Size: 378 4930
FseI GG_CCGG'CC
Cuts at: 2212 2212
Size: 5308
FspI TGC'GCA
Cuts at: 2300 3814 2300
Size: 1514 3794
HaeII r_GCGC'y
Cuts at: 1678 2033 2160 2297 4455 1678
Size: 355 127 137 2158 2531
Fragments arranged by size:
2531 2158 355 137 127
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3949 3981 4023 4055 1373
Size: 32 135 32 2377 32 42 32 2626
Fragments arranged by size:
2626 2377 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2652 2898 669
Size: 988 281 714 246 3079
Fragments arranged by size:
3079 988 714 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5308
HpaI GTT'AAC
Cuts at: 2898 2898
Size: 5308
KasI G'GCGC_C
Cuts at: 2029 2156 2029
Size: 127 5181
KpnI G_GTAC'C
Cuts at: 2633 2633
Size: 5308
MfeI C'AATT_G
Cuts at: 2907 2907
Size: 5308
MluI A'CGCG_T
Cuts at: 5184 5184
Size: 5308
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2052 2133 4422 4606 1305
Size: 747 81 2289 184 2007
Fragments arranged by size:
2289 2007 747 184 81
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3486
Fragments arranged by size:
3486 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3266 3625 3784 518
Size: 648 2100 359 159 2042
Fragments arranged by size:
2100 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2068 2088 2233 2724 3401 4549 4794 1606
Size: 462 20 145 491 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 491 462 245 145 20
NaeI GCC'GGC
Cuts at: 2210 2470 2210
Size: 260 5048
NarI GG'CG_CC
Cuts at: 2030 2157 2030
Size: 127 5181
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4756
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5308
NgoMIV G'CCGG_C
Cuts at: 2208 2468 2208
Size: 260 5048
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5308
NotI GC'GGCC_GC
Cuts at: 2755 2755
Size: 5308
NspI r_CATG'y
Cuts at: 1132 1229 2666 4211 1132
Size: 97 1437 1545 2229
Fragments arranged by size:
2229 1545 1437 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5308
PciI A'CATG_T
Cuts at: 4207 4207
Size: 5308
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5308
PmeI GTTT'AAAC
Cuts at: 2612 2612
Size: 5308
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3377 3409 4890 4922 3377
Size: 32 1481 32 3763
Fragments arranged by size:
3763 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5308
PsiI TTA'TAA
Cuts at: 2878 2878
Size: 5308
PspOMI G'GGCC_C
Cuts at: 2441 2441
Size: 5308
PstI C_TGCA'G
Cuts at: 830 2660 830
Size: 1830 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5308
SacII CC_GC'GG
Cuts at: 2089 2089
Size: 5308
SalI G'TCGA_C
Cuts at: 1936 2650 1936
Size: 714 4594
SbfI CC_TGCA'GG
Cuts at: 2660 2660
Size: 5308
ScaI AGT'ACT
Cuts at: 3556 5268 3556
Size: 1712 3596
SfcI C'TryA_G
Cuts at: 826 1045 2656 3791 4472 4663 826
Size: 219 1611 1135 681 191 1471
Fragments arranged by size:
1611 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2214 2214
Size: 5308
SfoI GGC'GCC
Cuts at: 2031 2158 2031
Size: 127 5181
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5308
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5308
SmaI CCC'GGG
Cuts at: 1872 2635 1872
Size: 763 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3412 4313 4575 820
Size: 197 926 1469 901 262 1553
Fragments arranged by size:
1553 1469 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3099 493
Size: 2606 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5308
SphI G_CATG'C
Cuts at: 1132 2666 1132
Size: 1534 3774
SspI AAT'ATT
Cuts at: 5 52 3232 5
Size: 47 3180 2081
Fragments arranged by size:
3180 2081 47
StuI AGG'CCT
Cuts at: 1517 2411 1517
Size: 894 4414
StyI C'CwwG_G
Cuts at: 513 1065 2747 513
Size: 552 1682 3074
Fragments arranged by size:
3074 1682 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2537 3335 3352 3505 3690 4135 325
Size: 1255 957 798 17 153 185 445 1498
Fragments arranged by size:
1498 1255 957 798 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3554 5266 96
Size: 274 80 33 51 1443 1577 1712 138
Fragments arranged by size:
1712 1577 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5268 5300 5268
Size: 32 5276
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5308
XbaI T'CTAG_A
Cuts at: 2644 2644
Size: 5308
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5308
XmaI C'CCGG_G
Cuts at: 1870 2633 1870
Size: 763 4545
XmnI GAAnn'nnTTC
Cuts at: 3437 3437
Size: 5308
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3116 276
Size: 53 83 186 936 1582 2468
Fragments arranged by size:
2468 1582 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FseI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI SacII SalI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I
EcoRV FalI FspAI NruI NsiI PacI PfoI PmlI
PpuMI PspXI PsrI PvuII RsrII SacI SanDI SapI
SexAI SrfI SwaI XcmI