Restriction map for
pFN21AB8873
(Circular) (Six-base) MAPSORT of: pFN21AB8873.seq Check: 5680 from: 1 to: 5191
pFN21AB8873 5191 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2133 2133
Size: 5191
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3001 278
Size: 53 83 186 936 1465 2468
Fragments arranged by size:
2468 1465 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2534 1937
Size: 597 4594
Acc65I G'GTAC_C
Cuts at: 2512 2512
Size: 5191
AclI AA'CG_TT
Cuts at: 1563 3318 3691 1563
Size: 1755 373 3063
Fragments arranged by size:
3063 1755 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2180 3254 4638 4906 1632
Size: 548 1074 1384 268 1917
Fragments arranged by size:
1917 1384 1074 548 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4994
AflIII A'CryG_T
Cuts at: 1203 4090 5067 1203
Size: 2887 977 1327
Fragments arranged by size:
2887 1327 977
AgeI A'CCGG_T
Cuts at: 4022 4022
Size: 5191
AhdI GACnn_n'nnGTC
Cuts at: 3920 3920
Size: 5191
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5191
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5191
AlwNI CAG_nnn'CTG
Cuts at: 2125 2934 4506 2125
Size: 809 1572 2810
Fragments arranged by size:
2810 1572 809
ApaI G_GGCC'C
Cuts at: 2399 2421 2399
Size: 22 5169
ApaLI G'TGCA_C
Cuts at: 3248 4404 3248
Size: 1156 4035
ApoI r'AATT_y
Cuts at: 1493 2500 2726 2878 4987 1493
Size: 1007 226 152 2109 1697
Fragments arranged by size:
2109 1697 1007 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3745 160
Size: 872 2713 1606
Fragments arranged by size:
2713 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5191
AvaI C'yCGr_G
Cuts at: 1870 1943 2391 2516 1870
Size: 73 448 125 4545
Fragments arranged by size:
4545 448 125 73
BamHI G'GATC_C
Cuts at: 2521 2888 2521
Size: 367 4824
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2066 2383 2512 3967 618
Size: 325 217 627 279 317 129 1455 1842
Fragments arranged by size:
1842 1455 627 325 317 279 217 129
BanII G_rGCy'C
Cuts at: 1391 2316 2399 2421 2510 1391
Size: 925 83 22 89 4072
Fragments arranged by size:
4072 925 89 83 22
BbeI G_GCGC'C
Cuts at: 2387 2387
Size: 5191
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4220
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3382 3416 1903
Size: 34 1445 34 3678
Fragments arranged by size:
3678 1445 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3083 4293 3083
Size: 1210 3981
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5191
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3802 136
Size: 107 122 71 631 605 2130 1525
Fragments arranged by size:
2130 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5186 5186
Size: 5191
BlpI GC'TnA_GC
Cuts at: 2608 2608
Size: 5191
Bme1580I G_kGCm'C
Cuts at: 1790 2399 2421 3252 4408 1790
Size: 609 22 831 1156 2573
Fragments arranged by size:
2573 1156 831 609 22
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5191
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2922 3880 442
Size: 416 53 83 399 24 1505 958 1753
Fragments arranged by size:
1753 1505 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5191
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2261 2330 3851 1239
Size: 1022 69 1521 2579
Fragments arranged by size:
2579 1521 1022 69
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2165 3316 4181 4479 2165
Size: 1151 865 298 2877
Fragments arranged by size:
2877 1151 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3854 882
Size: 617 2355 2219
Fragments arranged by size:
2355 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2982 493
Size: 711 1778 2702
Fragments arranged by size:
2702 1778 711
BsaBI GATnn'nnATC
Cuts at: 5185 5185
Size: 5191
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2384 2998 3380 4015
Size: 53 83 186 936 851 614 382 635
Cuts at: 4015 275
Size: 1451
Fragments arranged by size:
1451 936 851 635 614 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3624 4022 4296 4443 4854 3624
Size: 398 274 147 411 3961
Fragments arranged by size:
3961 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4966
Fragments arranged by size:
4966 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2099 2159 2249 1186
Size: 913 60 90 4128
Fragments arranged by size:
4128 913 90 60
BseYI C'CCAG_C
Cuts at: 4394 4890 4394
Size: 496 4695
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4618
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2489 2641 3402 4430 1540
Size: 275 186 488 152 761 1028 2301
Fragments arranged by size:
2301 1028 761 488 275 186 152
BsiHKAI G_wGCw'C
Cuts at: 2316 3252 3337 4408 2316
Size: 936 85 1071 3099
Fragments arranged by size:
3099 1071 936 85
BsmI GAATG_Cn'
Cuts at: 2702 2795 2702
Size: 93 5098
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2316 2399 2421 2510 3252 3337 4408
Size: 399 526 83 22 89 742 85 1071
Cuts at: 4408 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 526 399 89 85 83
22
BspEI T'CCGG_A
Cuts at: 4854 4854
Size: 5191
BspHI T'CATG_A
Cuts at: 3080 4810 3080
Size: 1730 3461
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2133 2546 844
Size: 1289 413 3489
Fragments arranged by size:
3489 1289 413
BsrBI CCG'CTC
Cuts at: 1394 1823 3078 1394
Size: 429 1255 3507
Fragments arranged by size:
3507 1255 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3686 3860 65
Size: 1183 2438 174 1396
Fragments arranged by size:
2438 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2169 2408 3835 4022 1700
Size: 110 359 239 1427 187 2869
Fragments arranged by size:
2869 1427 359 239 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5191
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5191
BssSI C'ACGA_G
Cuts at: 3251 4263 3251
Size: 1012 4179
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5191
BstBI TT'CG_AA
Cuts at: 3995 3995
Size: 5191
BstEII G'GTnAC_C
Cuts at: 1184 2092 1184
Size: 908 4283
BstYI r'GATC_y
Cuts at: 1972 2521 2888 3273 3290 4742 4828 4840 5186
Size: 549 367 385 17 1452 86 12 346
Cuts at: 5186 1972
Size: 1977
Fragments arranged by size:
1977 1452 549 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2188 2379 513
Size: 552 1123 191 3325
Fragments arranged by size:
3325 1123 552 191
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4452
Fragments arranged by size:
4452 683 56
BtsI GCAGTG_nn'
Cuts at: 2707 3501 3521 2707
Size: 794 20 4377
Fragments arranged by size:
4377 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5191
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5156
DraI TTT'AAA
Cuts at: 2495 2495
Size: 5191
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4198 809
Size: 507 2882 1802
Fragments arranged by size:
2882 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2439 2638 3527
Size: 54 1425 50 275 18 609 199 889
Cuts at: 3527 5156 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 609 275 199 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2638 1537
Size: 275 826 4090
Fragments arranged by size:
4090 826 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3121 1353
Size: 1768 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2270 3778 4152 4298 387
Size: 1064 138 681 1508 374 146 1280
Fragments arranged by size:
1508 1280 1064 681 374 146 138
EcoICRI GAG'CTC
Cuts at: 2314 2314
Size: 5191
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2180 2261 2330 3254 3851 4638 4906
Size: 393 548 81 69 924 597 787 268
Cuts at: 4906 1239
Size: 1524
Fragments arranged by size:
1524 924 787 597 548 393 268 81
69
EcoNI CCTnn'n_nnAGG
Cuts at: 2138 2138
Size: 5191
EcoO109I rG'GnC_Cy
Cuts at: 2131 2395 2396 2417 2418 2131
Size: 264 1 21 1 4904
Fragments arranged by size:
4904 264 21 1 1
EcoRI G'AATT_C
Cuts at: 2500 2878 2500
Size: 378 4813
FspI TGC'GCA
Cuts at: 3697 3697
Size: 5191
HaeII r_GCGC'y
Cuts at: 1678 2170 2345 2387 4338 1678
Size: 492 175 42 1951 2531
Fragments arranged by size:
2531 1951 492 175 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2471 2503 3832 3864 3906
Size: 32 135 32 899 32 1329 32 42
Cuts at: 3906 3938 1373
Size: 32 2626
Fragments arranged by size:
2626 1329 899 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2535 2781 669
Size: 988 281 597 246 3079
Fragments arranged by size:
3079 988 597 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5191
HpaI GTT'AAC
Cuts at: 2781 2781
Size: 5191
KasI G'GCGC_C
Cuts at: 2383 2383
Size: 5191
KpnI G_GTAC'C
Cuts at: 2516 2516
Size: 5191
MfeI C'AATT_G
Cuts at: 2790 2790
Size: 5191
MluI A'CGCG_T
Cuts at: 5067 5067
Size: 5191
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4305 4489 1305
Size: 3000 184 2007
Fragments arranged by size:
3000 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3369
Fragments arranged by size:
3369 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2102 3149 3508 3667 518
Size: 648 936 1047 359 159 2042
Fragments arranged by size:
2042 1047 936 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2158 2190 2205 2607 3284 4432 4677 1606
Size: 552 32 15 402 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 552 402 245 32 15
NaeI GCC'GGC
Cuts at: 2171 2410 2171
Size: 239 4952
NarI GG'CG_CC
Cuts at: 2384 2384
Size: 5191
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4639
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5191
NgoMIV G'CCGG_C
Cuts at: 2169 2408 2169
Size: 239 4952
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5191
NotI GC'GGCC_GC
Cuts at: 2638 2638
Size: 5191
NspI r_CATG'y
Cuts at: 1132 1229 2549 4094 1132
Size: 97 1320 1545 2229
Fragments arranged by size:
2229 1545 1320 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5191
PciI A'CATG_T
Cuts at: 4090 4090
Size: 5191
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5191
PmeI GTTT'AAAC
Cuts at: 2495 2495
Size: 5191
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3260 3292 4773 4805 3260
Size: 32 1481 32 3646
Fragments arranged by size:
3646 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5191
PsiI TTA'TAA
Cuts at: 2761 2761
Size: 5191
PspOMI G'GGCC_C
Cuts at: 2395 2417 2395
Size: 22 5169
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2177 2209 2177
Size: 32 5159
PstI C_TGCA'G
Cuts at: 830 2265 2349 2543 830
Size: 1435 84 194 3478
Fragments arranged by size:
3478 1435 194 84
PvuI CG_AT'CG
Cuts at: 2001 2489 2001
Size: 488 4703
PvuII CAG'CTG
Cuts at: 2158 2205 2158
Size: 47 5144
SacI G_AGCT'C
Cuts at: 2316 2316
Size: 5191
SacII CC_GC'GG
Cuts at: 2191 2191
Size: 5191
SalI G'TCGA_C
Cuts at: 1936 2533 1936
Size: 597 4594
SbfI CC_TGCA'GG
Cuts at: 2543 2543
Size: 5191
ScaI AGT'ACT
Cuts at: 3439 5151 3439
Size: 1712 3479
SfcI C'TryA_G
Cuts at: 826 1045 2261 2345 2539 3674 4355 4546 826
Size: 219 1216 84 194 1135 681 191 1471
Fragments arranged by size:
1471 1216 1135 681 219 194 191 84
SfoI GGC'GCC
Cuts at: 2385 2385
Size: 5191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5191
SgrAI Cr'CCGG_yG
Cuts at: 1700 2169 1700
Size: 469 4722
SmaI CCC'GGG
Cuts at: 1872 2393 2518 1872
Size: 521 125 4545
Fragments arranged by size:
4545 521 125
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2180 3295 4196 4458 820
Size: 197 926 237 1115 901 262 1553
Fragments arranged by size:
1553 1115 926 901 262 237 197
SnaBI TAC'GTA
Cuts at: 493 2982 493
Size: 2489 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5191
SphI G_CATG'C
Cuts at: 1132 2549 1132
Size: 1417 3774
SspI AAT'ATT
Cuts at: 5 52 3115 5
Size: 47 3063 2081
Fragments arranged by size:
3063 2081 47
StuI AGG'CCT
Cuts at: 1517 2033 1517
Size: 516 4675
StyI C'CwwG_G
Cuts at: 513 1065 2139 2630 513
Size: 552 1074 491 3074
Fragments arranged by size:
3074 1074 552 491
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3218 3235 3388 3573 4018 325
Size: 1255 1638 17 153 185 445 1498
Fragments arranged by size:
1638 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3437 5149 96
Size: 274 80 33 51 1443 1460 1712 138
Fragments arranged by size:
1712 1460 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5151 5183 5151
Size: 32 5159
Tth111I GACn'n_nGTC
Cuts at: 1316 2098 1316
Size: 782 4409
XbaI T'CTAG_A
Cuts at: 2527 2527
Size: 5191
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2305 2305
Size: 5191
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5191
XmaI C'CCGG_G
Cuts at: 1870 2391 2516 1870
Size: 521 125 4545
Fragments arranged by size:
4545 521 125
XmnI GAAnn'nnTTC
Cuts at: 3320 3320
Size: 5191
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2999 276
Size: 53 83 186 936 1465 2468
Fragments arranged by size:
2468 1465 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
SacI SacII SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV
FalI FseI FspAI NruI NsiI PacI PfoI PmlI
PpuMI PspXI RsrII SanDI SapI SexAI SfiI SrfI
SwaI