Restriction map for
pFN21AB8870
(Circular) (Six-base) MAPSORT of: pFN21AB8870.seq Check: 412 from: 1 to: 5107
pFN21AB8870 5107 bp
With 182 enzymes: SgfI *
October 20, 2009 10:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2917 278
Size: 53 83 186 936 1381 2468
Fragments arranged by size:
2468 1381 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2450 1937
Size: 513 4594
Acc65I G'GTAC_C
Cuts at: 2428 2428
Size: 5107
AclI AA'CG_TT
Cuts at: 1563 3234 3607 1563
Size: 1671 373 3063
Fragments arranged by size:
3063 1671 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 1999 3170 4554 4822 1632
Size: 367 1171 1384 268 1917
Fragments arranged by size:
1917 1384 1171 367 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4910
AflIII A'CryG_T
Cuts at: 1203 4006 4983 1203
Size: 2803 977 1327
Fragments arranged by size:
2803 1327 977
AgeI A'CCGG_T
Cuts at: 3938 3938
Size: 5107
AhdI GACnn_n'nnGTC
Cuts at: 3836 3836
Size: 5107
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5107
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5107
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2282 2314 2282
Size: 32 5075
AlwNI CAG_nnn'CTG
Cuts at: 2850 4422 2850
Size: 1572 3535
ApaLI G'TGCA_C
Cuts at: 3164 4320 3164
Size: 1156 3951
ApoI r'AATT_y
Cuts at: 1493 2360 2416 2642 2794 4903 1493
Size: 867 56 226 152 2109 1697
Fragments arranged by size:
2109 1697 867 226 152 56
AseI AT'TA_AT
Cuts at: 160 1032 3661 160
Size: 872 2629 1606
Fragments arranged by size:
2629 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5107
AvaI C'yCGr_G
Cuts at: 1870 1943 2282 2432 1870
Size: 73 339 150 4545
Fragments arranged by size:
4545 339 150 73
BamHI G'GATC_C
Cuts at: 2437 2804 2437
Size: 367 4740
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2152 2428 3883 618
Size: 325 217 627 365 276 1455 1842
Fragments arranged by size:
1842 1455 627 365 325 276 217
BanII G_rGCy'C
Cuts at: 1391 2426 1391
Size: 1035 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4136
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2089 2123 3298 3332 1903
Size: 34 152 34 1175 34 3678
Fragments arranged by size:
3678 1175 152 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2999 4209 2999
Size: 1210 3897
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5107
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3718 136
Size: 107 122 71 631 605 2046 1525
Fragments arranged by size:
2046 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5102 5102
Size: 5107
BlpI GC'TnA_GC
Cuts at: 2524 2524
Size: 5107
Bme1580I G_kGCm'C
Cuts at: 1790 2157 3168 4324 1790
Size: 367 1011 1156 2573
Fragments arranged by size:
2573 1156 1011 367
BmgBI CAC'GTC
Cuts at: 1318 2206 1318
Size: 888 4219
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2838 3796 442
Size: 416 53 83 399 24 1421 958 1753
Fragments arranged by size:
1753 1421 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5107
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3767 1239
Size: 2528 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2126 3232 4097 4395 2126
Size: 1106 865 298 2838
Fragments arranged by size:
2838 1106 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2272 3770 882
Size: 617 773 1498 2219
Fragments arranged by size:
2219 1498 773 617
BsaAI yAC'GTr
Cuts at: 493 1204 2898 493
Size: 711 1694 2702
Fragments arranged by size:
2702 1694 711
BsaBI GATnn'nnATC
Cuts at: 2192 5101 2192
Size: 2909 2198
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2297 2306 2914 3296
Size: 53 83 186 936 764 9 608 382
Cuts at: 3296 3931 275
Size: 635 1451
Fragments arranged by size:
1451 936 764 635 608 382 186 83
53 9
BsaWI w'CCGG_w
Cuts at: 3540 3938 4212 4359 4770 3540
Size: 398 274 147 411 3877
Fragments arranged by size:
3877 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2117 2147 1178
Size: 30 165 30 714 30 4138
Fragments arranged by size:
4138 714 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5107
BseYI C'CCAG_C
Cuts at: 2156 4310 4806 2156
Size: 2154 496 2457
Fragments arranged by size:
2457 2154 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4534
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2557 3318 4346 1540
Size: 275 186 556 761 1028 2301
Fragments arranged by size:
2301 1028 761 556 275 186
BsiHKAI G_wGCw'C
Cuts at: 3168 3253 4324 3168
Size: 85 1071 3951
Fragments arranged by size:
3951 1071 85
BsmI GAATG_Cn'
Cuts at: 2618 2711 2618
Size: 93 5014
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2157 2426 3168 3253 4324 1391
Size: 399 367 269 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 367 269 85
BspEI T'CCGG_A
Cuts at: 4770 4770
Size: 5107
BspHI T'CATG_A
Cuts at: 2996 4726 2996
Size: 1730 3377
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2235 2462 844
Size: 1391 227 3489
Fragments arranged by size:
3489 1391 227
BsrBI CCG'CTC
Cuts at: 1394 1823 2994 1394
Size: 429 1171 3507
Fragments arranged by size:
3507 1171 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3602 3776 65
Size: 1183 2354 174 1396
Fragments arranged by size:
2354 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3751 3938 1700
Size: 110 1941 187 2869
Fragments arranged by size:
2869 1941 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5107
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5107
BssSI C'ACGA_G
Cuts at: 3167 4179 3167
Size: 1012 4095
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5107
BstBI TT'CG_AA
Cuts at: 3911 3911
Size: 5107
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5107
BstYI r'GATC_y
Cuts at: 1972 2437 2804 3189 3206 4658 4744 4756 5102
Size: 465 367 385 17 1452 86 12 346
Cuts at: 5102 1972
Size: 1977
Fragments arranged by size:
1977 1452 465 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4555
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4368
Fragments arranged by size:
4368 683 56
BtsI GCAGTG_nn'
Cuts at: 2623 3417 3437 2623
Size: 794 20 4293
Fragments arranged by size:
4293 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5107
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5072
DraI TTT'AAA
Cuts at: 2411 2411
Size: 5107
DraIII CAC_nnn'GTG
Cuts at: 2402 2402
Size: 5107
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4114 809
Size: 507 2798 1802
Fragments arranged by size:
2798 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2554 3443 5072
Size: 54 1425 50 275 18 724 889 1629
Cuts at: 5072 8
Size: 43
Fragments arranged by size:
1629 1425 889 724 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2554 1537
Size: 275 742 4090
Fragments arranged by size:
4090 742 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3037 1353
Size: 1684 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3694 4068 4214 387
Size: 1064 138 2105 374 146 1280
Fragments arranged by size:
2105 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1999 3170 3767 4554 4822 1239
Size: 393 367 1171 597 787 268 1524
Fragments arranged by size:
1524 1171 787 597 393 367 268
EcoRI G'AATT_C
Cuts at: 2360 2416 2794 2360
Size: 56 378 4673
Fragments arranged by size:
4673 378 56
EcoRV GAT'ATC
Cuts at: 2332 2332
Size: 5107
FspI TGC'GCA
Cuts at: 3613 3613
Size: 5107
HaeII r_GCGC'y
Cuts at: 1678 4254 1678
Size: 2576 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2117 2149 2363 2395 3748
Size: 32 135 32 545 32 214 32 1353
Cuts at: 3748 3780 3822 3854 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1353 545 214 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2451 2697 669
Size: 988 281 513 246 3079
Fragments arranged by size:
3079 988 513 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5107
HpaI GTT'AAC
Cuts at: 2697 2697
Size: 5107
KpnI G_GTAC'C
Cuts at: 2432 2432
Size: 5107
MfeI C'AATT_G
Cuts at: 2706 2706
Size: 5107
MluI A'CGCG_T
Cuts at: 4983 4983
Size: 5107
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2031 4221 4405 1305
Size: 726 2190 184 2007
Fragments arranged by size:
2190 2007 726 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3285
Fragments arranged by size:
3285 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3065 3424 3583 518
Size: 648 1899 359 159 2042
Fragments arranged by size:
2042 1899 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2311 2523 3200 4348 4593 1606
Size: 705 212 677 1148 245 2120
Fragments arranged by size:
2120 1148 705 677 245 212
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4555
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5107
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5107
NotI GC'GGCC_GC
Cuts at: 2554 2554
Size: 5107
NspI r_CATG'y
Cuts at: 1132 1229 2465 4010 1132
Size: 97 1236 1545 2229
Fragments arranged by size:
2229 1545 1236 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5107
PciI A'CATG_T
Cuts at: 4006 4006
Size: 5107
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2062 1108
Size: 954 4153
PfoI T'CCnGG_A
Cuts at: 2072 2300 2072
Size: 228 4879
PmeI GTTT'AAAC
Cuts at: 2411 2411
Size: 5107
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3176 3208 4689 4721 3176
Size: 32 1481 32 3562
Fragments arranged by size:
3562 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5107
PsiI TTA'TAA
Cuts at: 2677 2677
Size: 5107
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2114 2146 2114
Size: 32 5075
PstI C_TGCA'G
Cuts at: 830 2202 2232 2459 830
Size: 1372 30 227 3478
Fragments arranged by size:
3478 1372 227 30
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5107
SalI G'TCGA_C
Cuts at: 1936 2449 1936
Size: 513 4594
SbfI CC_TGCA'GG
Cuts at: 2459 2459
Size: 5107
ScaI AGT'ACT
Cuts at: 3355 5067 3355
Size: 1712 3395
SfcI C'TryA_G
Cuts at: 826 1045 2198 2228 2455 3590 4271 4462 826
Size: 219 1153 30 227 1135 681 191 1471
Fragments arranged by size:
1471 1153 1135 681 227 219 191 30
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5107
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5107
SmaI CCC'GGG
Cuts at: 1872 2284 2434 1872
Size: 412 150 4545
Fragments arranged by size:
4545 412 150
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2141 3211 4112 4374 820
Size: 197 926 198 1070 901 262 1553
Fragments arranged by size:
1553 1070 926 901 262 198 197
SnaBI TAC'GTA
Cuts at: 493 2898 493
Size: 2405 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5107
SphI G_CATG'C
Cuts at: 1132 2465 1132
Size: 1333 3774
SspI AAT'ATT
Cuts at: 5 52 3031 5
Size: 47 2979 2081
Fragments arranged by size:
2979 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5107
StyI C'CwwG_G
Cuts at: 513 1065 2546 513
Size: 552 1481 3074
Fragments arranged by size:
3074 1481 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3134 3151 3304 3489 3934 325
Size: 1255 1554 17 153 185 445 1498
Fragments arranged by size:
1554 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3353 5065 96
Size: 274 80 33 51 1443 1376 1712 138
Fragments arranged by size:
1712 1443 1376 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5067 5099 5067
Size: 32 5075
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5107
XbaI T'CTAG_A
Cuts at: 2443 2443
Size: 5107
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5107
XmaI C'CCGG_G
Cuts at: 1870 2282 2432 1870
Size: 412 150 4545
Fragments arranged by size:
4545 412 150
XmnI GAAnn'nnTTC
Cuts at: 2012 3236 2012
Size: 1224 3883
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2915 276
Size: 53 83 186 936 1381 2468
Fragments arranged by size:
2468 1381 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI