Restriction map for
pFN21AB8867
(Circular) (Six-base) MAPSORT of: pFN21AB8867.seq Check: 5667 from: 1 to: 5071
pFN21AB8867 5071 bp
With 182 enzymes: SgfI *
October 20, 2009 10:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2059 2188 2059
Size: 129 4942
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2881 278
Size: 53 83 186 936 1345 2468
Fragments arranged by size:
2468 1345 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2414 1937
Size: 477 4594
Acc65I G'GTAC_C
Cuts at: 2392 2392
Size: 5071
AclI AA'CG_TT
Cuts at: 1563 3198 3571 1563
Size: 1635 373 3063
Fragments arranged by size:
3063 1635 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3134 4518 4786 1632
Size: 1502 1384 268 1917
Fragments arranged by size:
1917 1502 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4874
AflIII A'CryG_T
Cuts at: 1203 3970 4947 1203
Size: 2767 977 1327
Fragments arranged by size:
2767 1327 977
AgeI A'CCGG_T
Cuts at: 3902 3902
Size: 5071
AhdI GACnn_n'nnGTC
Cuts at: 2233 3800 2233
Size: 1567 3504
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5071
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5071
AlwNI CAG_nnn'CTG
Cuts at: 2280 2814 4386 2280
Size: 534 1572 2965
Fragments arranged by size:
2965 1572 534
ApaLI G'TGCA_C
Cuts at: 3128 4284 3128
Size: 1156 3915
ApoI r'AATT_y
Cuts at: 1493 2380 2606 2758 4867 1493
Size: 887 226 152 2109 1697
Fragments arranged by size:
2109 1697 887 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3625 160
Size: 872 2593 1606
Fragments arranged by size:
2593 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5071
AvaI C'yCGr_G
Cuts at: 1870 1943 2396 1870
Size: 73 453 4545
Fragments arranged by size:
4545 453 73
BamHI G'GATC_C
Cuts at: 2401 2768 2401
Size: 367 4704
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2392 3847 618
Size: 325 217 627 605 1455 1842
Fragments arranged by size:
1842 1455 627 605 325 217
BanII G_rGCy'C
Cuts at: 1391 2093 2390 1391
Size: 702 297 4072
Fragments arranged by size:
4072 702 297
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2232 928
Size: 971 333 3767
Fragments arranged by size:
3767 971 333
BbvCI CC'TCA_GC
Cuts at: 2273 2273
Size: 5071
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3262 3296 1903
Size: 34 50 34 1241 34 3678
Fragments arranged by size:
3678 1241 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2963 4173 2963
Size: 1210 3861
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5071
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2326 3682 136
Size: 107 122 71 631 605 654 1356 1525
Fragments arranged by size:
1525 1356 654 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5066 5066
Size: 5071
BlpI GC'TnA_GC
Cuts at: 2488 2488
Size: 5071
Bme1580I G_kGCm'C
Cuts at: 1790 3132 4288 1790
Size: 1342 1156 2573
Fragments arranged by size:
2573 1342 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5071
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2802 3760 442
Size: 416 53 83 399 24 1385 958 1753
Fragments arranged by size:
1753 1385 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5071
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3731 1239
Size: 2492 2579
Bpu10I CC'TnA_GC
Cuts at: 2273 2273
Size: 5071
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3196 4061 4359 3196
Size: 865 298 3908
Fragments arranged by size:
3908 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3734 882
Size: 617 2235 2219
Fragments arranged by size:
2235 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2862 493
Size: 711 1658 2702
Fragments arranged by size:
2702 1658 711
BsaBI GATnn'nnATC
Cuts at: 5065 5065
Size: 5071
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2878 3260 3895 275
Size: 53 83 186 936 1345 382 635 1451
Fragments arranged by size:
1451 1345 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3504 3902 4176 4323 4734 3504
Size: 398 274 147 411 3841
Fragments arranged by size:
3841 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4846
Fragments arranged by size:
4846 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2167 1186
Size: 981 4090
BseYI C'CCAG_C
Cuts at: 2022 2214 4274 4770 2022
Size: 192 2060 496 2323
Fragments arranged by size:
2323 2060 496 192
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4498
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2521 3282 4310 1540
Size: 275 186 520 761 1028 2301
Fragments arranged by size:
2301 1028 761 520 275 186
BsiHKAI G_wGCw'C
Cuts at: 2166 3132 3217 4288 2166
Size: 966 85 1071 2949
Fragments arranged by size:
2949 1071 966 85
BsmI GAATG_Cn'
Cuts at: 2582 2675 2582
Size: 93 4978
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2093 2166 2390 3132 3217 4288 1391
Size: 399 303 73 224 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 303 224 85 73
BspEI T'CCGG_A
Cuts at: 4734 4734
Size: 5071
BspHI T'CATG_A
Cuts at: 2960 4690 2960
Size: 1730 3341
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2059 2188 2426 844
Size: 1215 129 238 3489
Fragments arranged by size:
3489 1215 238 129
BsrBI CCG'CTC
Cuts at: 1394 1823 2958 1394
Size: 429 1135 3507
Fragments arranged by size:
3507 1135 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3566 3740 65
Size: 1183 2318 174 1396
Fragments arranged by size:
2318 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3715 3902 1700
Size: 110 1905 187 2869
Fragments arranged by size:
2869 1905 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5071
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5071
BssSI C'ACGA_G
Cuts at: 3131 4143 3131
Size: 1012 4059
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5071
BstBI TT'CG_AA
Cuts at: 2157 3875 2157
Size: 1718 3353
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5071
BstXI CCAn_nnnn'nTGG
Cuts at: 2302 2302
Size: 5071
BstYI r'GATC_y
Cuts at: 1972 2401 2768 3153 3170 4622 4708 4720 5066
Size: 429 367 385 17 1452 86 12 346
Cuts at: 5066 1972
Size: 1977
Fragments arranged by size:
1977 1452 429 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4519
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4332
Fragments arranged by size:
4332 683 56
BtsI GCAGTG_nn'
Cuts at: 2043 2587 3381 3401 2043
Size: 544 794 20 3713
Fragments arranged by size:
3713 794 544 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5071
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5036
DraI TTT'AAA
Cuts at: 2375 2375
Size: 5071
DraIII CAC_nnn'GTG
Cuts at: 2170 2170
Size: 5071
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4078 809
Size: 507 2762 1802
Fragments arranged by size:
2762 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2292 2518 3407
Size: 54 1425 50 275 18 462 226 889
Cuts at: 3407 5036 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 462 275 226 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2518 1537
Size: 275 706 4090
Fragments arranged by size:
4090 706 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2154 3001 1353
Size: 801 847 3423
Fragments arranged by size:
3423 847 801
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2344 3658 4032 4178 387
Size: 1064 138 755 1314 374 146 1280
Fragments arranged by size:
1314 1280 1064 755 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3134 3731 4518 4786 1239
Size: 393 1502 597 787 268 1524
Fragments arranged by size:
1524 1502 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2096 2096
Size: 5071
EcoRI G'AATT_C
Cuts at: 2380 2758 2380
Size: 378 4693
FspI TGC'GCA
Cuts at: 3577 3577
Size: 5071
HaeII r_GCGC'y
Cuts at: 1678 4218 1678
Size: 2540 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2219 2251 3712 3744 3786
Size: 32 135 32 647 32 1461 32 42
Cuts at: 3786 3818 1373
Size: 32 2626
Fragments arranged by size:
2626 1461 647 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2415 2661 669
Size: 988 281 477 246 3079
Fragments arranged by size:
3079 988 477 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5071
HpaI GTT'AAC
Cuts at: 2661 2661
Size: 5071
KpnI G_GTAC'C
Cuts at: 2396 2396
Size: 5071
MfeI C'AATT_G
Cuts at: 2670 2670
Size: 5071
MluI A'CGCG_T
Cuts at: 4947 4947
Size: 5071
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2371 4185 4369 1305
Size: 1066 1814 184 2007
Fragments arranged by size:
2007 1814 1066 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2294 10
Size: 54 1425 343 462 2787
Fragments arranged by size:
2787 1425 462 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3029 3388 3547 518
Size: 648 1863 359 159 2042
Fragments arranged by size:
2042 1863 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2249 2487 3164 4312 4557 1606
Size: 643 238 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 643 245 238
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4519
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5071
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5071
NotI GC'GGCC_GC
Cuts at: 2518 2518
Size: 5071
NspI r_CATG'y
Cuts at: 1132 1229 2429 3974 1132
Size: 97 1200 1545 2229
Fragments arranged by size:
2229 1545 1200 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5071
PciI A'CATG_T
Cuts at: 3970 3970
Size: 5071
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5071
PmeI GTTT'AAAC
Cuts at: 2375 2375
Size: 5071
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3140 3172 4653 4685 3140
Size: 32 1481 32 3526
Fragments arranged by size:
3526 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5071
PsiI TTA'TAA
Cuts at: 2641 2641
Size: 5071
PstI C_TGCA'G
Cuts at: 830 2033 2070 2137 2423 830
Size: 1203 37 67 286 3478
Fragments arranged by size:
3478 1203 286 67 37
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5071
PvuII CAG'CTG
Cuts at: 2249 2249
Size: 5071
SalI G'TCGA_C
Cuts at: 1936 2413 1936
Size: 477 4594
SapI GCTCTTCn'nnn_
Cuts at: 2154 2154
Size: 5071
SbfI CC_TGCA'GG
Cuts at: 2423 2423
Size: 5071
ScaI AGT'ACT
Cuts at: 3319 5031 3319
Size: 1712 3359
SfcI C'TryA_G
Cuts at: 826 1045 2029 2066 2133 2419 3554 4235 4426
Size: 219 984 37 67 286 1135 681 191
Cuts at: 4426 826
Size: 1471
Fragments arranged by size:
1471 1135 984 681 286 219 191 67
37
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5071
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5071
SmaI CCC'GGG
Cuts at: 1872 2398 1872
Size: 526 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3175 4076 4338 820
Size: 197 926 1232 901 262 1553
Fragments arranged by size:
1553 1232 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2862 493
Size: 2369 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5071
SphI G_CATG'C
Cuts at: 1132 2429 1132
Size: 1297 3774
SspI AAT'ATT
Cuts at: 5 52 2995 5
Size: 47 2943 2081
Fragments arranged by size:
2943 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5071
StyI C'CwwG_G
Cuts at: 513 1065 2510 513
Size: 552 1445 3074
Fragments arranged by size:
3074 1445 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2174 3098 3115 3268 3453 3898 325
Size: 1255 594 924 17 153 185 445 1498
Fragments arranged by size:
1498 1255 924 594 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3317 5029 96
Size: 274 80 33 51 1443 1340 1712 138
Fragments arranged by size:
1712 1443 1340 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5031 5063 5031
Size: 32 5039
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5071
XbaI T'CTAG_A
Cuts at: 2407 2407
Size: 5071
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5071
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5071
XmaI C'CCGG_G
Cuts at: 1870 2396 1870
Size: 526 4545
XmnI GAAnn'nnTTC
Cuts at: 3200 3200
Size: 5071
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2879 276
Size: 53 83 186 936 1345 2468
Fragments arranged by size:
2468 1345 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI