Restriction map for
pFN21AB8842
(Circular) (Six-base) MAPSORT of: pFN21AB8842.seq Check: 823 from: 1 to: 6160
pFN21AB8842 6160 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3186 3186
Size: 6160
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3970 278
Size: 53 83 186 936 2434 2468
Fragments arranged by size:
2468 2434 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2340 2645 3503 1937
Size: 403 305 858 4594
Fragments arranged by size:
4594 858 403 305
Acc65I G'GTAC_C
Cuts at: 2813 3481 2813
Size: 668 5492
AclI AA'CG_TT
Cuts at: 1563 4287 4660 1563
Size: 2724 373 3063
Fragments arranged by size:
3063 2724 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2391 4223 5607 5875 1632
Size: 759 1832 1384 268 1917
Fragments arranged by size:
1917 1832 1384 759 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5963
AflIII A'CryG_T
Cuts at: 1203 5059 6036 1203
Size: 3856 977 1327
Fragments arranged by size:
3856 1327 977
AgeI A'CCGG_T
Cuts at: 4991 4991
Size: 6160
AhdI GACnn_n'nnGTC
Cuts at: 4889 4889
Size: 6160
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6160
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2889 1184
Size: 1705 4455
AlwNI CAG_nnn'CTG
Cuts at: 2902 3106 3903 5475 2902
Size: 204 797 1572 3587
Fragments arranged by size:
3587 1572 797 204
ApaLI G'TGCA_C
Cuts at: 4217 5373 4217
Size: 1156 5004
ApoI r'AATT_y
Cuts at: 1493 3469 3695 3847 5956 1493
Size: 1976 226 152 2109 1697
Fragments arranged by size:
2109 1976 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4714 160
Size: 872 3682 1606
Fragments arranged by size:
3682 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6160
AvaI C'yCGr_G
Cuts at: 1870 1943 2304 2331 2684 2777 3485 1870
Size: 73 361 27 353 93 708 4545
Fragments arranged by size:
4545 708 361 353 93 73 27
BamHI G'GATC_C
Cuts at: 2446 3490 3857 2446
Size: 1044 367 4749
Fragments arranged by size:
4749 1044 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2813 3481 4936 618
Size: 325 217 627 1026 668 1455 1842
Fragments arranged by size:
1842 1455 1026 668 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2118 3479 1391
Size: 727 1361 4072
Fragments arranged by size:
4072 1361 727
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5189
BbvCI CC'TCA_GC
Cuts at: 2496 2798 2496
Size: 302 5858
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4351 4385 1903
Size: 34 50 34 2330 34 3678
Fragments arranged by size:
3678 2330 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4052 5262 4052
Size: 1210 4950
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6160
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4771 136
Size: 107 122 71 631 605 3099 1525
Fragments arranged by size:
3099 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2235 6155 2235
Size: 3920 2240
BlpI GC'TnA_GC
Cuts at: 2614 3577 2614
Size: 963 5197
Bme1580I G_kGCm'C
Cuts at: 1790 2911 3214 4221 5377 1790
Size: 1121 303 1007 1156 2573
Fragments arranged by size:
2573 1156 1121 1007 303
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6160
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2167 3891 4849
Size: 416 53 83 399 24 750 1724 958
Cuts at: 4849 442
Size: 1753
Fragments arranged by size:
1753 1724 958 750 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6160
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2710 2710
Size: 6160
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2336 2519 2655 2758 2978 3212 4820 1239
Size: 1097 183 136 103 220 234 1608 2579
Fragments arranged by size:
2579 1608 1097 234 220 183 136 103
Bpu10I CC'TnA_GC
Cuts at: 2496 2654 2798 2496
Size: 158 144 5858
Fragments arranged by size:
5858 158 144
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2030 2116 4285 5150 5448 2030
Size: 86 2169 865 298 2742
Fragments arranged by size:
2742 2169 865 298 86
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3302 4823 882
Size: 617 1803 1521 2219
Fragments arranged by size:
2219 1803 1521 617
BsaAI yAC'GTr
Cuts at: 493 1204 3951 493
Size: 711 2747 2702
Fragments arranged by size:
2747 2702 711
BsaBI GATnn'nnATC
Cuts at: 6154 6154
Size: 6160
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3967 4349 4984 275
Size: 53 83 186 936 2434 382 635 1451
Fragments arranged by size:
2434 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2250 4593 4991 5265 5412 5823 2250
Size: 2343 398 274 147 411 2587
Fragments arranged by size:
2587 2343 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5935
Fragments arranged by size:
5935 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3362 3398 1186
Size: 2176 36 3948
Fragments arranged by size:
3948 2176 36
BseYI C'CCAG_C
Cuts at: 3132 5363 5859 3132
Size: 2231 496 3433
Fragments arranged by size:
3433 2231 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2135 2330 2989 3146 3358 1163
Size: 573 399 195 659 157 212 3965
Fragments arranged by size:
3965 659 573 399 212 195 157
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2716 3610 4371 5399 1540
Size: 275 186 715 894 761 1028 2301
Fragments arranged by size:
2301 1028 894 761 715 275 186
BsiHKAI G_wGCw'C
Cuts at: 2606 2615 3228 4221 4306 5377 2606
Size: 9 613 993 85 1071 3389
Fragments arranged by size:
3389 1071 993 613 85 9
BsmI GAATG_Cn'
Cuts at: 3671 3764 3671
Size: 93 6067
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2118 2606 2615 2911 3214 3228 3479
Size: 399 328 488 9 296 303 14 251
Cuts at: 3479 4221 4306 5377 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 488 399 328 303 296
251 85 14 9
BspEI T'CCGG_A
Cuts at: 5823 5823
Size: 6160
BspHI T'CATG_A
Cuts at: 4049 5779 4049
Size: 1730 4430
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3186 3439 3515 844
Size: 2342 253 76 3489
Fragments arranged by size:
3489 2342 253 76
BsrBI CCG'CTC
Cuts at: 1394 1823 3394 4047 1394
Size: 429 1571 653 3507
Fragments arranged by size:
3507 1571 653 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4655 4829 65
Size: 1183 3407 174 1396
Fragments arranged by size:
3407 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3244 4804 4991 1700
Size: 110 1434 1560 187 2869
Fragments arranged by size:
2869 1560 1434 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6160
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6160
BssSI C'ACGA_G
Cuts at: 4220 5232 4220
Size: 1012 5148
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2610 3266 794
Size: 1816 656 3688
Fragments arranged by size:
3688 1816 656
BstBI TT'CG_AA
Cuts at: 4964 4964
Size: 6160
BstEII G'GTnAC_C
Cuts at: 1184 2782 1184
Size: 1598 4562
BstXI CCAn_nnnn'nTGG
Cuts at: 2443 2810 2443
Size: 367 5793
BstYI r'GATC_y
Cuts at: 1972 2235 2446 3102 3490 3857 4242 4259 5711
Size: 263 211 656 388 367 385 17 1452
Cuts at: 5711 5797 5809 6155 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 656 388 385 367 346 263
211 86 17 12
BstZ17I GTA'TAC
Cuts at: 2646 2646
Size: 6160
BtgI C'CryG_G
Cuts at: 513 1065 2027 2091 2571 513
Size: 552 962 64 480 4102
Fragments arranged by size:
4102 962 552 480 64
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5421
Fragments arranged by size:
5421 683 56
BtsI GCAGTG_nn'
Cuts at: 2462 3336 3370 3676 4470 4490 2462
Size: 874 34 306 794 20 4132
Fragments arranged by size:
4132 874 794 306 34 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6160
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6125
DraI TTT'AAA
Cuts at: 3464 3464
Size: 6160
DraIII CAC_nnn'GTG
Cuts at: 2610 2610
Size: 6160
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5167 809
Size: 507 3851 1802
Fragments arranged by size:
3851 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3420 3607 4496
Size: 54 1425 50 275 18 1590 187 889
Cuts at: 4496 6125 8
Size: 1629 43
Fragments arranged by size:
1629 1590 1425 889 275 187 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3607 1537
Size: 275 1795 4090
Fragments arranged by size:
4090 1795 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4090 1353
Size: 2737 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4747 5121 5267 387
Size: 1064 138 3158 374 146 1280
Fragments arranged by size:
3158 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2336 2391 2519 2655 2758 2978 3212
Size: 393 704 55 128 136 103 220 234
Cuts at: 3212 4223 4820 5607 5875 1239
Size: 1011 597 787 268 1524
Fragments arranged by size:
1524 1011 787 704 597 393 268 234
220 136 128 103 55
EcoNI CCTnn'n_nnAGG
Cuts at: 2123 3344 2123
Size: 1221 4939
EcoO109I rG'GnC_Cy
Cuts at: 2500 2899 3169 2500
Size: 399 270 5491
Fragments arranged by size:
5491 399 270
EcoRI G'AATT_C
Cuts at: 3469 3847 3469
Size: 378 5782
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2905 2905
Size: 6160
FspI TGC'GCA
Cuts at: 4666 4666
Size: 6160
HaeII r_GCGC'y
Cuts at: 1678 5307 1678
Size: 3629 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4801 4833 4875 4907 1373
Size: 32 135 32 3229 32 42 32 2626
Fragments arranged by size:
3229 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2161 3504 3750 669
Size: 988 281 223 1343 246 3079
Fragments arranged by size:
3079 1343 988 281 246 223
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6160
HpaI GTT'AAC
Cuts at: 2161 3750 2161
Size: 1589 4571
KpnI G_GTAC'C
Cuts at: 2817 3485 2817
Size: 668 5492
MfeI C'AATT_G
Cuts at: 3759 3759
Size: 6160
MluI A'CGCG_T
Cuts at: 6036 6036
Size: 6160
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3478 5274 5458 1305
Size: 2173 1796 184 2007
Fragments arranged by size:
2173 2007 1796 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4338
Fragments arranged by size:
4338 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2441 4118 4477 4636 518
Size: 648 1275 1677 359 159 2042
Fragments arranged by size:
2042 1677 1275 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2036 2314 2495 2554 2736 2965 3147 3153
Size: 430 278 181 59 182 229 182 6
Cuts at: 3153 3361 3429 3576 4253 5401 5646 1606
Size: 208 68 147 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 430 278 245 229 208
182 182 181 147 68 59 6
NaeI GCC'GGC
Cuts at: 3246 3246
Size: 6160
NcoI C'CATG_G
Cuts at: 513 1065 2091 513
Size: 552 1026 4582
Fragments arranged by size:
4582 1026 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6160
NgoMIV G'CCGG_C
Cuts at: 3244 3244
Size: 6160
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6160
NotI GC'GGCC_GC
Cuts at: 3607 3607
Size: 6160
NspI r_CATG'y
Cuts at: 1132 1229 2863 3241 3518 5063 1132
Size: 97 1634 378 277 1545 2229
Fragments arranged by size:
2229 1634 1545 378 277 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6160
PciI A'CATG_T
Cuts at: 5059 5059
Size: 6160
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6160
PfoI T'CCnGG_A
Cuts at: 3105 3105
Size: 6160
PmeI GTTT'AAAC
Cuts at: 3464 3464
Size: 6160
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4229 4261 5742 5774 4229
Size: 32 1481 32 4615
Fragments arranged by size:
4615 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2500 2500
Size: 6160
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6160
PsiI TTA'TAA
Cuts at: 3730 3730
Size: 6160
PspXI vC'TCGA_Gb
Cuts at: 2777 2777
Size: 6160
PstI C_TGCA'G
Cuts at: 830 2601 2878 3152 3512 830
Size: 1771 277 274 360 3478
Fragments arranged by size:
3478 1771 360 277 274
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6160
PvuII CAG'CTG
Cuts at: 2036 2314 2495 2554 2965 3147 3153 3361 3429
Size: 278 181 59 411 182 6 208 68
Cuts at: 3429 2036
Size: 4767
Fragments arranged by size:
4767 411 278 208 182 181 68 59
6
SalI G'TCGA_C
Cuts at: 1936 3502 1936
Size: 1566 4594
SbfI CC_TGCA'GG
Cuts at: 3512 3512
Size: 6160
ScaI AGT'ACT
Cuts at: 4408 6120 4408
Size: 1712 4448
SexAI A'CCwGG_T
Cuts at: 2154 2154
Size: 6160
SfcI C'TryA_G
Cuts at: 826 1045 2597 2874 3148 3508 4643 5324 5515
Size: 219 1552 277 274 360 1135 681 191
Cuts at: 5515 826
Size: 1471
Fragments arranged by size:
1552 1471 1135 681 360 277 274 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6160
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6160
SmaI CCC'GGG
Cuts at: 1872 3487 1872
Size: 1615 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2009 2131 2777 4264 5165 5427
Size: 197 926 66 122 646 1487 901 262
Cuts at: 5427 820
Size: 1553
Fragments arranged by size:
1553 1487 926 901 646 262 197 122
66
SnaBI TAC'GTA
Cuts at: 493 3951 493
Size: 3458 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6160
SphI G_CATG'C
Cuts at: 1132 3518 1132
Size: 2386 3774
SspI AAT'ATT
Cuts at: 5 52 4084 5
Size: 47 4032 2081
Fragments arranged by size:
4032 2081 47
StuI AGG'CCT
Cuts at: 1517 2376 1517
Size: 859 5301
StyI C'CwwG_G
Cuts at: 513 1065 2062 2091 2264 2358 3378 3599 513
Size: 552 997 29 173 94 1020 221 3074
Fragments arranged by size:
3074 1020 997 552 221 173 94 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2804 4187 4204 4357 4542 4987 325
Size: 1255 1224 1383 17 153 185 445 1498
Fragments arranged by size:
1498 1383 1255 1224 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4406 6118 96
Size: 274 80 33 51 1443 2429 1712 138
Fragments arranged by size:
2429 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2292 2324 2454 2486 6120 6152 2292
Size: 32 130 32 3634 32 2300
Fragments arranged by size:
3634 2300 130 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6160
XbaI T'CTAG_A
Cuts at: 3277 3496 3277
Size: 219 5941
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2089 2089
Size: 6160
XhoI C'TCGA_G
Cuts at: 1943 2777 1943
Size: 834 5326
XmaI C'CCGG_G
Cuts at: 1870 3485 1870
Size: 1615 4545
XmnI GAAnn'nnTTC
Cuts at: 4289 4289
Size: 6160
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3968 276
Size: 53 83 186 936 2434 2468
Fragments arranged by size:
2468 2434 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI
PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI Bsu36I EcoICRI EcoRV FseI FspAI KasI
NarI NruI NsiI PacI PmlI PspOMI PsrI RsrII
SacI SacII SanDI SapI SfiI SfoI SrfI SwaI