Restriction map for
pFN21AB8840
(Circular) (Six-base) MAPSORT of: pFN21AB8840.seq Check: 5215 from: 1 to: 6106
pFN21AB8840 6106 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3916 278
Size: 53 83 186 936 2380 2468
Fragments arranged by size:
2468 2380 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2511 3449 1937
Size: 574 938 4594
Fragments arranged by size:
4594 938 574
Acc65I G'GTAC_C
Cuts at: 3427 3427
Size: 6106
AclI AA'CG_TT
Cuts at: 1563 4233 4606 1563
Size: 2670 373 3063
Fragments arranged by size:
3063 2670 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2462 3296 4169 5553 5821 1632
Size: 830 834 873 1384 268 1917
Fragments arranged by size:
1917 1384 873 834 830 268
AflII C'TTAA_G
Cuts at: 820 1017 2558 820
Size: 197 1541 4368
Fragments arranged by size:
4368 1541 197
AflIII A'CryG_T
Cuts at: 1203 5005 5982 1203
Size: 3802 977 1327
Fragments arranged by size:
3802 1327 977
AgeI A'CCGG_T
Cuts at: 4937 4937
Size: 6106
AhdI GACnn_n'nnGTC
Cuts at: 4835 4835
Size: 6106
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6106
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2286 2469 1184
Size: 1102 183 4821
Fragments arranged by size:
4821 1102 183
AlwNI CAG_nnn'CTG
Cuts at: 2167 2605 3849 5421 2167
Size: 438 1244 1572 2852
Fragments arranged by size:
2852 1572 1244 438
ApaI G_GGCC'C
Cuts at: 2711 3231 2711
Size: 520 5586
ApaLI G'TGCA_C
Cuts at: 2042 4163 5319 2042
Size: 2121 1156 2829
Fragments arranged by size:
2829 2121 1156
ApoI r'AATT_y
Cuts at: 1493 3415 3641 3793 5902 1493
Size: 1922 226 152 2109 1697
Fragments arranged by size:
2109 1922 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4660 160
Size: 872 3628 1606
Fragments arranged by size:
3628 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6106
AvaI C'yCGr_G
Cuts at: 1870 1943 2466 2931 3431 1870
Size: 73 523 465 500 4545
Fragments arranged by size:
4545 523 500 465 73
BamHI G'GATC_C
Cuts at: 3342 3436 3803 3342
Size: 94 367 5645
Fragments arranged by size:
5645 367 94
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2582 2763 2834 3427 4882
Size: 325 217 627 795 181 71 593 1455
Cuts at: 4882 618
Size: 1842
Fragments arranged by size:
1842 1455 795 627 593 325 217 181
71
BanII G_rGCy'C
Cuts at: 1391 2438 2711 2825 3231 3337 3425 1391
Size: 1047 273 114 406 106 88 4072
Fragments arranged by size:
4072 1047 406 273 114 106 88
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5135
BbvCI CC'TCA_GC
Cuts at: 3183 3183
Size: 6106
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4297 4331 1903
Size: 34 2360 34 3678
Fragments arranged by size:
3678 2360 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3998 5208 3998
Size: 1210 4896
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6106
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2138 2900 3068
Size: 107 122 71 631 605 466 762 168
Cuts at: 3068 4717 136
Size: 1649 1525
Fragments arranged by size:
1649 1525 762 631 605 466 168 122
107 71
BglII A'GATC_T
Cuts at: 3196 6101 3196
Size: 2905 3201
BlpI GC'TnA_GC
Cuts at: 2432 3336 3523 2432
Size: 904 187 5015
Fragments arranged by size:
5015 904 187
Bme1580I G_kGCm'C
Cuts at: 1790 2046 2275 2711 2766 2837 2890 3231 4167
Size: 256 229 436 55 71 53 341 936
Cuts at: 4167 5323 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 936 436 341 256 229 71
55 53
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6106
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2793 2799 3837
Size: 416 53 83 399 24 1376 6 1038
Cuts at: 3837 4795 442
Size: 958 1753
Fragments arranged by size:
1753 1376 1038 958 416 399 83 53
24 6
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6106
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2283 3362 4766 1239
Size: 1044 1079 1404 2579
Fragments arranged by size:
2579 1404 1079 1044
Bpu10I CC'TnA_GC
Cuts at: 2925 2964 2976 3114 3183 2925
Size: 39 12 138 69 5848
Fragments arranged by size:
5848 138 69 39 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2561 4231 5096 5394 2561
Size: 1670 865 298 3273
Fragments arranged by size:
3273 1670 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4769 882
Size: 617 3270 2219
Fragments arranged by size:
3270 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3897 493
Size: 711 2693 2702
Fragments arranged by size:
2702 2693 711
BsaBI GATnn'nnATC
Cuts at: 6100 6100
Size: 6106
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3913 4295 4930 275
Size: 53 83 186 936 2380 382 635 1451
Fragments arranged by size:
2380 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4539 4937 5211 5358 5769 4539
Size: 398 274 147 411 4876
Fragments arranged by size:
4876 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2281 2311 1178
Size: 30 165 30 878 30 4973
Fragments arranged by size:
4973 878 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2619 3200 1186
Size: 1433 581 4092
Fragments arranged by size:
4092 1433 581
BseYI C'CCAG_C
Cuts at: 2142 3078 5309 5805 2142
Size: 936 2231 496 2443
Fragments arranged by size:
2443 2231 936 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2493 1163
Size: 573 757 4776
Fragments arranged by size:
4776 757 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3556 4317 5345 1540
Size: 275 186 1555 761 1028 2301
Fragments arranged by size:
2301 1555 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2046 2311 2438 4167 4252 5323 2046
Size: 265 127 1729 85 1071 2829
Fragments arranged by size:
2829 1729 1071 265 127 85
BsmI GAATG_Cn'
Cuts at: 3617 3710 3617
Size: 93 6013
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2046 2275 2311 2438 2711 2766 2825
Size: 399 256 229 36 127 273 55 59
Cuts at: 2825 2837 2890 3231 3337 3425 4167 4252 5323
Size: 12 53 341 106 88 742 85 1071
Cuts at: 5323 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 341 273 256 229
127 106 88 85 59 55 53 36
12
BspEI T'CCGG_A
Cuts at: 5769 5769
Size: 6106
BspHI T'CATG_A
Cuts at: 3995 5725 3995
Size: 1730 4376
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2327 2433 3461 844
Size: 1483 106 1028 3489
Fragments arranged by size:
3489 1483 1028 106
BsrBI CCG'CTC
Cuts at: 1394 1823 3993 1394
Size: 429 2170 3507
Fragments arranged by size:
3507 2170 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3383 4601 4775 65
Size: 1183 2135 1218 174 1396
Fragments arranged by size:
2135 1396 1218 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4750 4937 1700
Size: 110 2940 187 2869
Fragments arranged by size:
2940 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6106
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6106
BssSI C'ACGA_G
Cuts at: 2268 2883 4166 5178 2268
Size: 615 1283 1012 3196
Fragments arranged by size:
3196 1283 1012 615
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2167 794
Size: 1373 4733
BstBI TT'CG_AA
Cuts at: 4910 4910
Size: 6106
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6106
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 3010 2011
Size: 999 5107
BstYI r'GATC_y
Cuts at: 1972 2122 3107 3196 3342 3436 3803 4188 4205
Size: 150 985 89 146 94 367 385 17
Cuts at: 4205 5657 5743 5755 6101 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 985 385 367 346 150 146
94 89 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3240 3240
Size: 6106
BtgI C'CryG_G
Cuts at: 513 1065 2064 513
Size: 552 999 4555
Fragments arranged by size:
4555 999 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5367
Fragments arranged by size:
5367 683 56
BtsI GCAGTG_nn'
Cuts at: 2093 2209 2348 2484 2603 3622 4416 4436 2093
Size: 116 139 136 119 1019 794 20 3763
Fragments arranged by size:
3763 1019 794 139 136 119 116 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6106
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6071
DraI TTT'AAA
Cuts at: 3410 3410
Size: 6106
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5113 809
Size: 507 3797 1802
Fragments arranged by size:
3797 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2379 2871 3553
Size: 54 1425 50 275 18 549 492 682
Cuts at: 3553 4442 6071 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 682 549 492 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3553 1537
Size: 275 1741 4090
Fragments arranged by size:
4090 1741 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2082 3164 3206 4036 1353
Size: 729 1082 42 830 3423
Fragments arranged by size:
3423 1082 830 729 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4693 5067 5213 387
Size: 1064 138 3104 374 146 1280
Fragments arranged by size:
3104 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2436 2436
Size: 6106
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2283 2462 3296 3362 4169 4766 5553
Size: 393 651 179 834 66 807 597 787
Cuts at: 5553 5821 1239
Size: 268 1524
Fragments arranged by size:
1524 834 807 787 651 597 393 268
179 66
EcoNI CCTnn'n_nnAGG
Cuts at: 2087 2087
Size: 6106
EcoO109I rG'GnC_Cy
Cuts at: 2080 2164 2545 2707 2708 2732 2793 3228 3254
Size: 84 381 162 1 24 61 435 26
Cuts at: 3254 2080
Size: 4932
Fragments arranged by size:
4932 435 381 162 84 61 26 24
1
EcoRI G'AATT_C
Cuts at: 3415 3793 3415
Size: 378 5728
FspI TGC'GCA
Cuts at: 2201 4612 2201
Size: 2411 3695
HaeII r_GCGC'y
Cuts at: 1678 5253 1678
Size: 3575 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2107 2139 3290 3322 4747
Size: 32 135 32 535 32 1151 32 1425
Cuts at: 4747 4779 4821 4853 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1425 1151 535 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3450 3696 669
Size: 988 281 1512 246 3079
Fragments arranged by size:
3079 1512 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6106
HpaI GTT'AAC
Cuts at: 3696 3696
Size: 6106
KpnI G_GTAC'C
Cuts at: 3431 3431
Size: 6106
MfeI C'AATT_G
Cuts at: 3705 3705
Size: 6106
MluI A'CGCG_T
Cuts at: 5982 5982
Size: 6106
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2209 5220 5404 1305
Size: 904 3011 184 2007
Fragments arranged by size:
3011 2007 904 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2381 2873 10
Size: 54 1425 343 549 492 3243
Fragments arranged by size:
3243 1425 549 492 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 4064 4423 4582 518
Size: 648 843 2055 359 159 2042
Fragments arranged by size:
2055 2042 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3331 3340 3522 4199 5347 5592 1606
Size: 1725 9 182 677 1148 245 2120
Fragments arranged by size:
2120 1725 1148 677 245 182 9
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5554
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6106
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6106
NotI GC'GGCC_GC
Cuts at: 3553 3553
Size: 6106
NspI r_CATG'y
Cuts at: 1132 1229 2027 2414 3464 5009 1132
Size: 97 798 387 1050 1545 2229
Fragments arranged by size:
2229 1545 1050 798 387 97
PasI CC'CwG_GG
Cuts at: 1348 2735 2830 3084 1348
Size: 1387 95 254 4370
Fragments arranged by size:
4370 1387 254 95
PciI A'CATG_T
Cuts at: 5005 5005
Size: 6106
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2149 1108
Size: 1041 5065
PmeI GTTT'AAAC
Cuts at: 3410 3410
Size: 6106
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4175 4207 5688 5720 4175
Size: 32 1481 32 4561
Fragments arranged by size:
4561 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2793 2793
Size: 6106
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6106
PsiI TTA'TAA
Cuts at: 3676 3676
Size: 6106
PspOMI G'GGCC_C
Cuts at: 2707 3227 2707
Size: 520 5586
PstI C_TGCA'G
Cuts at: 830 2163 2338 2610 3458 830
Size: 1333 175 272 848 3478
Fragments arranged by size:
3478 1333 848 272 175
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6106
SacI G_AGCT'C
Cuts at: 2438 2438
Size: 6106
SalI G'TCGA_C
Cuts at: 1936 3448 1936
Size: 1512 4594
SanDI GG'GwC_CC
Cuts at: 2793 2793
Size: 6106
SbfI CC_TGCA'GG
Cuts at: 2163 2338 3458 2163
Size: 175 1120 4811
Fragments arranged by size:
4811 1120 175
ScaI AGT'ACT
Cuts at: 4354 6066 4354
Size: 1712 4394
SfcI C'TryA_G
Cuts at: 826 1045 2159 2221 2334 2606 3454 4589 5270
Size: 219 1114 62 113 272 848 1135 681
Cuts at: 5270 5461 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1114 848 681 272 219 191
113 62
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6106
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6106
SmaI CCC'GGG
Cuts at: 1872 2468 2933 3433 1872
Size: 596 465 500 4545
Fragments arranged by size:
4545 596 500 465
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2540 2558 4210 5111 5373 820
Size: 197 926 597 18 1652 901 262 1553
Fragments arranged by size:
1652 1553 926 901 597 262 197 18
SnaBI TAC'GTA
Cuts at: 493 3897 493
Size: 3404 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6106
SphI G_CATG'C
Cuts at: 1132 2414 3464 1132
Size: 1282 1050 3774
Fragments arranged by size:
3774 1282 1050
SrfI GCCC'GGGC
Cuts at: 2933 2933
Size: 6106
SspI AAT'ATT
Cuts at: 5 52 4030 5
Size: 47 3978 2081
Fragments arranged by size:
3978 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6106
StyI C'CwwG_G
Cuts at: 513 1065 2853 2895 2916 2937 2943 2997 3024
Size: 552 1788 42 21 21 6 54 27
Cuts at: 3024 3030 3042 3057 3063 3545 513
Size: 6 12 15 6 482 3074
Fragments arranged by size:
3074 1788 552 482 54 42 27 21
21 15 12 6 6 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2507 2570 4133 4150 4303 4488 4933
Size: 1255 927 63 1563 17 153 185 445
Cuts at: 4933 325
Size: 1498
Fragments arranged by size:
1563 1498 1255 927 445 185 153 63
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4352 6064 96
Size: 274 80 33 51 1443 2375 1712 138
Fragments arranged by size:
2375 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2224 2256 2281 2313 2338 2370 6066 6098 2224
Size: 32 25 32 25 32 3696 32 2232
Fragments arranged by size:
3696 2232 32 32 32 32 25 25
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6106
XbaI T'CTAG_A
Cuts at: 3442 3442
Size: 6106
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2575 2950 3064 2011
Size: 564 375 114 5053
Fragments arranged by size:
5053 564 375 114
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6106
XmaI C'CCGG_G
Cuts at: 1870 2466 2931 3431 1870
Size: 596 465 500 4545
Fragments arranged by size:
4545 596 500 465
XmnI GAAnn'nnTTC
Cuts at: 4235 4235
Size: 6106
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3914 276
Size: 53 83 186 936 2380 2468
Fragments arranged by size:
2468 2380 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI SacI SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspXI PsrI PvuII RsrII SacII SapI
SexAI SfiI SfoI SwaI