Restriction map for
pFN21AB8839
(Circular) (Six-base) MAPSORT of: pFN21AB8839.seq Check: 6975 from: 1 to: 6103
pFN21AB8839 6103 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3913 278
Size: 53 83 186 936 2377 2468
Fragments arranged by size:
2468 2377 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3446 1937
Size: 1509 4594
Acc65I G'GTAC_C
Cuts at: 3424 3424
Size: 6103
AclI AA'CG_TT
Cuts at: 1563 4230 4603 1563
Size: 2667 373 3063
Fragments arranged by size:
3063 2667 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3036 4166 5550 5818 1632
Size: 1404 1130 1384 268 1917
Fragments arranged by size:
1917 1404 1384 1130 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5906
AflIII A'CryG_T
Cuts at: 1203 5002 5979 1203
Size: 3799 977 1327
Fragments arranged by size:
3799 1327 977
AgeI A'CCGG_T
Cuts at: 4934 4934
Size: 6103
AhdI GACnn_n'nnGTC
Cuts at: 4832 4832
Size: 6103
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6103
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6103
AlwNI CAG_nnn'CTG
Cuts at: 3846 5418 3846
Size: 1572 4531
ApaI G_GGCC'C
Cuts at: 3208 3208
Size: 6103
ApaLI G'TGCA_C
Cuts at: 3243 4160 5316 3243
Size: 917 1156 4030
Fragments arranged by size:
4030 1156 917
ApoI r'AATT_y
Cuts at: 1493 3412 3638 3790 5899 1493
Size: 1919 226 152 2109 1697
Fragments arranged by size:
2109 1919 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4657 160
Size: 872 3625 1606
Fragments arranged by size:
3625 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6103
AvaI C'yCGr_G
Cuts at: 1870 1943 2634 3144 3428 1870
Size: 73 691 510 284 4545
Fragments arranged by size:
4545 691 510 284 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2359 2392 2998 3031 2359
Size: 33 606 33 5431
Fragments arranged by size:
5431 606 33 33
BamHI G'GATC_C
Cuts at: 2096 2806 3433 3800 2096
Size: 710 627 367 4399
Fragments arranged by size:
4399 710 627 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2987 3424 4879 618
Size: 325 217 627 1200 437 1455 1842
Fragments arranged by size:
1842 1455 1200 627 437 325 217
BanII G_rGCy'C
Cuts at: 1391 2473 2852 3208 3422 1391
Size: 1082 379 356 214 4072
Fragments arranged by size:
4072 1082 379 356 214
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3042 3356 928
Size: 971 1143 314 3675
Fragments arranged by size:
3675 1143 971 314
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4294 4328 1903
Size: 34 2357 34 3678
Fragments arranged by size:
3678 2357 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3995 5205 3995
Size: 1210 4893
BclI T'GATC_A
Cuts at: 1548 2601 1548
Size: 1053 5050
BfrBI ATG'CAT
Cuts at: 2976 2976
Size: 6103
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4714 136
Size: 107 122 71 631 605 3042 1525
Fragments arranged by size:
3042 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6098 6098
Size: 6103
BlpI GC'TnA_GC
Cuts at: 3520 3520
Size: 6103
Bme1580I G_kGCm'C
Cuts at: 1790 3208 3247 4164 5320 1790
Size: 1418 39 917 1156 2573
Fragments arranged by size:
2573 1418 1156 917 39
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6103
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2393 3834 4792
Size: 416 53 83 399 24 976 1441 958
Cuts at: 4792 442
Size: 1753
Fragments arranged by size:
1753 1441 976 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2306 1056
Size: 1250 4853
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2150 2457 2789 4763 1239
Size: 911 307 332 1974 2579
Fragments arranged by size:
2579 1974 911 332 307
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4228 5093 5391 4228
Size: 865 298 4940
Fragments arranged by size:
4940 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4766 882
Size: 617 3267 2219
Fragments arranged by size:
3267 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3894 493
Size: 711 2690 2702
Fragments arranged by size:
2702 2690 711
BsaBI GATnn'nnATC
Cuts at: 6097 6097
Size: 6103
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3910 4292 4927 275
Size: 53 83 186 936 2377 382 635 1451
Fragments arranged by size:
2377 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4536 4934 5208 5355 5766 4536
Size: 398 274 147 411 4873
Fragments arranged by size:
4873 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5878
Fragments arranged by size:
5878 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2014 2852 3126 1186
Size: 828 838 274 4163
Fragments arranged by size:
4163 838 828 274
BseYI C'CCAG_C
Cuts at: 2750 5306 5802 2750
Size: 2556 496 3051
Fragments arranged by size:
3051 2556 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5530
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3553 4314 5342 1540
Size: 275 186 1552 761 1028 2301
Fragments arranged by size:
2301 1552 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2775 2852 3228 3247 4164 4249 5320 2775
Size: 77 376 19 917 85 1071 3558
Fragments arranged by size:
3558 1071 917 376 85 77 19
BsmI GAATG_Cn'
Cuts at: 3614 3707 3614
Size: 93 6010
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2473 2775 2852 3208 3228 3247 3422
Size: 399 683 302 77 356 20 19 175
Cuts at: 3422 4164 4249 5320 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 683 399 356 302 175
85 77 20 19
BspEI T'CCGG_A
Cuts at: 5766 5766
Size: 6103
BspHI T'CATG_A
Cuts at: 2607 3992 5722 2607
Size: 1385 1730 2988
Fragments arranged by size:
2988 1730 1385
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2414 3458 844
Size: 1570 1044 3489
Fragments arranged by size:
3489 1570 1044
BsrBI CCG'CTC
Cuts at: 1394 1823 3990 1394
Size: 429 2167 3507
Fragments arranged by size:
3507 2167 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2332 3340 4598 4772 65
Size: 1183 1084 1008 1258 174 1396
Fragments arranged by size:
1396 1258 1183 1084 1008 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4747 4934 1700
Size: 110 2937 187 2869
Fragments arranged by size:
2937 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2241 96
Size: 2145 3958
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6103
BssSI C'ACGA_G
Cuts at: 4163 5175 4163
Size: 1012 5091
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6103
BstBI TT'CG_AA
Cuts at: 4907 4907
Size: 6103
BstEII G'GTnAC_C
Cuts at: 1184 2110 1184
Size: 926 5177
BstXI CCAn_nnnn'nTGG
Cuts at: 2419 2938 2419
Size: 519 5584
BstYI r'GATC_y
Cuts at: 1972 2096 2806 3433 3800 4185 4202 5654 5740
Size: 124 710 627 367 385 17 1452 86
Cuts at: 5740 5752 6098 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 710 627 385 367 346 124
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3033 513
Size: 552 1968 3583
Fragments arranged by size:
3583 1968 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5364
Fragments arranged by size:
5364 683 56
BtsI GCAGTG_nn'
Cuts at: 2761 3619 4413 4433 2761
Size: 858 794 20 4431
Fragments arranged by size:
4431 858 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6103
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6068
DraI TTT'AAA
Cuts at: 3358 3407 3358
Size: 49 6054
DraIII CAC_nnn'GTG
Cuts at: 3267 3267
Size: 6103
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2318 5110 809
Size: 507 1002 2792 1802
Fragments arranged by size:
2792 1802 1002 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3550 4439 6068
Size: 54 1425 50 275 18 1720 889 1629
Cuts at: 6068 8
Size: 43
Fragments arranged by size:
1720 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3550 1537
Size: 275 1738 4090
Fragments arranged by size:
4090 1738 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2489 4033 1353
Size: 1136 1544 3423
Fragments arranged by size:
3423 1544 1136
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4690 5064 5210 387
Size: 1064 138 3101 374 146 1280
Fragments arranged by size:
3101 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2850 2850
Size: 6103
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2150 2457 2789 3036 4166 4763 5550
Size: 393 518 307 332 247 1130 597 787
Cuts at: 5550 5818 1239
Size: 268 1524
Fragments arranged by size:
1524 1130 787 597 518 393 332 307
268 247
EcoO109I rG'GnC_Cy
Cuts at: 2279 2449 3205 2279
Size: 170 756 5177
Fragments arranged by size:
5177 756 170
EcoRI G'AATT_C
Cuts at: 3412 3790 3412
Size: 378 5725
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3370 3370
Size: 6103
FspI TGC'GCA
Cuts at: 4609 4609
Size: 6103
HaeII r_GCGC'y
Cuts at: 1678 3396 5250 1678
Size: 1718 1854 2531
Fragments arranged by size:
2531 1854 1718
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2849 2881 2945 2977 4744
Size: 32 135 32 1277 32 64 32 1767
Cuts at: 4744 4776 4818 4850 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1767 1277 135 64 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2155 2176 3447 3693 669
Size: 988 281 217 21 1271 246 3079
Fragments arranged by size:
3079 1271 988 281 246 217 21
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6103
HpaI GTT'AAC
Cuts at: 3693 3693
Size: 6103
KpnI G_GTAC'C
Cuts at: 3428 3428
Size: 6103
MfeI C'AATT_G
Cuts at: 3273 3702 3273
Size: 429 5674
MluI A'CGCG_T
Cuts at: 5979 5979
Size: 6103
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5217 5401 1305
Size: 3912 184 2007
Fragments arranged by size:
3912 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4281
Fragments arranged by size:
4281 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2417 2612 4061 4420 4579 518
Size: 648 1251 195 1449 359 159 2042
Fragments arranged by size:
2042 1449 1251 648 359 195 159
MspA1I CmG'CkG
Cuts at: 1606 2441 3519 4196 5344 5589 1606
Size: 835 1078 677 1148 245 2120
Fragments arranged by size:
2120 1148 1078 835 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5551
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6103
NheI G'CTAG_C
Cuts at: 1052 2302 1052
Size: 1250 4853
NotI GC'GGCC_GC
Cuts at: 3550 3550
Size: 6103
NsiI A_TGCA'T
Cuts at: 2978 2978
Size: 6103
NspI r_CATG'y
Cuts at: 1132 1229 2749 2976 3461 5006 1132
Size: 97 1520 227 485 1545 2229
Fragments arranged by size:
2229 1545 1520 485 227 97
PasI CC'CwG_GG
Cuts at: 1348 2802 2938 1348
Size: 1454 136 4513
Fragments arranged by size:
4513 1454 136
PciI A'CATG_T
Cuts at: 5002 5002
Size: 6103
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6103
PmeI GTTT'AAAC
Cuts at: 3407 3407
Size: 6103
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4172 4204 5685 5717 4172
Size: 32 1481 32 4558
Fragments arranged by size:
4558 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2279 2449 2279
Size: 170 5933
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6103
PsiI TTA'TAA
Cuts at: 2058 3381 3673 2058
Size: 1323 292 4488
Fragments arranged by size:
4488 1323 292
PspOMI G'GGCC_C
Cuts at: 3204 3204
Size: 6103
PstI C_TGCA'G
Cuts at: 830 3455 830
Size: 2625 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6103
PvuII CAG'CTG
Cuts at: 2441 2441
Size: 6103
SacI G_AGCT'C
Cuts at: 2852 2852
Size: 6103
SalI G'TCGA_C
Cuts at: 1936 3445 1936
Size: 1509 4594
SbfI CC_TGCA'GG
Cuts at: 3455 3455
Size: 6103
ScaI AGT'ACT
Cuts at: 4351 6063 4351
Size: 1712 4391
SfcI C'TryA_G
Cuts at: 826 1045 3451 4586 5267 5458 826
Size: 219 2406 1135 681 191 1471
Fragments arranged by size:
2406 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6103
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6103
SmaI CCC'GGG
Cuts at: 1872 3146 3430 1872
Size: 1274 284 4545
Fragments arranged by size:
4545 1274 284
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4207 5108 5370 820
Size: 197 926 2264 901 262 1553
Fragments arranged by size:
2264 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3894 493
Size: 3401 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6103
SphI G_CATG'C
Cuts at: 1132 3461 1132
Size: 2329 3774
SspI AAT'ATT
Cuts at: 5 52 3082 4027 5
Size: 47 3030 945 2081
Fragments arranged by size:
3030 2081 945 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6103
StyI C'CwwG_G
Cuts at: 513 1065 3542 513
Size: 552 2477 3074
Fragments arranged by size:
3074 2477 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4130 4147 4300 4485 4930 325
Size: 1255 2550 17 153 185 445 1498
Fragments arranged by size:
2550 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2241 4349 6061
Size: 274 80 33 51 1443 264 2108 1712
Cuts at: 6061 96
Size: 138
Fragments arranged by size:
2108 1712 1443 274 264 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2271 2303 2513 2545 2709 2741 3237 3269 6063
Size: 32 210 32 164 32 496 32 2794
Cuts at: 6063 6095 2271
Size: 32 2279
Fragments arranged by size:
2794 2279 496 210 164 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6103
XbaI T'CTAG_A
Cuts at: 3439 3439
Size: 6103
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3169 3169
Size: 6103
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6103
XmaI C'CCGG_G
Cuts at: 1870 3144 3428 1870
Size: 1274 284 4545
Fragments arranged by size:
4545 1274 284
XmnI GAAnn'nnTTC
Cuts at: 4232 4232
Size: 6103
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3911 276
Size: 53 83 186 936 2377 2468
Fragments arranged by size:
2468 2377 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI
PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI