Restriction map for
pFN21AB8837
(Circular) (Six-base) MAPSORT of: pFN21AB8837.seq Check: 2262 from: 1 to: 6094
pFN21AB8837 6094 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2947 2963 2947
Size: 16 6078
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3904 278
Size: 53 83 186 936 2368 2468
Fragments arranged by size:
2468 2368 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2391 3147 3437 1937
Size: 454 756 290 4594
Fragments arranged by size:
4594 756 454 290
Acc65I G'GTAC_C
Cuts at: 3415 3415
Size: 6094
AclI AA'CG_TT
Cuts at: 1563 4221 4594 1563
Size: 2658 373 3063
Fragments arranged by size:
3063 2658 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2380 2542 4157 5541 5809 1632
Size: 748 162 1615 1384 268 1917
Fragments arranged by size:
1917 1615 1384 748 268 162
AflII C'TTAA_G
Cuts at: 820 1017 3347 820
Size: 197 2330 3567
Fragments arranged by size:
3567 2330 197
AflIII A'CryG_T
Cuts at: 1203 4993 5970 1203
Size: 3790 977 1327
Fragments arranged by size:
3790 1327 977
AgeI A'CCGG_T
Cuts at: 4925 4925
Size: 6094
AhdI GACnn_n'nnGTC
Cuts at: 4823 4823
Size: 6094
AleI CACnn'nnGTG
Cuts at: 1166 2510 2687 1166
Size: 1344 177 4573
Fragments arranged by size:
4573 1344 177
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6094
AlwNI CAG_nnn'CTG
Cuts at: 2140 2842 3837 5409 2140
Size: 702 995 1572 2825
Fragments arranged by size:
2825 1572 995 702
ApaLI G'TGCA_C
Cuts at: 4151 5307 4151
Size: 1156 4938
ApoI r'AATT_y
Cuts at: 1493 3403 3629 3781 5890 1493
Size: 1910 226 152 2109 1697
Fragments arranged by size:
2109 1910 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4648 160
Size: 872 3616 1606
Fragments arranged by size:
3616 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6094
AvaI C'yCGr_G
Cuts at: 1870 1943 3419 1870
Size: 73 1476 4545
Fragments arranged by size:
4545 1476 73
BamHI G'GATC_C
Cuts at: 3424 3791 3424
Size: 367 5727
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2029 2724 3062 3415 4870
Size: 325 217 627 242 695 338 353 1455
Cuts at: 4870 618
Size: 1842
Fragments arranged by size:
1842 1455 695 627 353 338 325 242
217
BanII G_rGCy'C
Cuts at: 1391 3287 3413 1391
Size: 1896 126 4072
Fragments arranged by size:
4072 1896 126
BbeI G_GCGC'C
Cuts at: 2728 3066 2728
Size: 338 5756
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5123
BbvCI CC'TCA_GC
Cuts at: 3183 3183
Size: 6094
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3316 3350 4285 4319 1903
Size: 34 1379 34 935 34 3678
Fragments arranged by size:
3678 1379 935 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3986 5196 3986
Size: 1210 4884
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6094
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2624 2733 2948
Size: 107 122 71 631 605 952 109 215
Cuts at: 2948 4705 136
Size: 1757 1525
Fragments arranged by size:
1757 1525 952 631 605 215 122 109
107 71
BglII A'GATC_T
Cuts at: 6089 6089
Size: 6094
BlpI GC'TnA_GC
Cuts at: 3511 3511
Size: 6094
Bme1580I G_kGCm'C
Cuts at: 1790 4155 5311 1790
Size: 2365 1156 2573
Fragments arranged by size:
2573 2365 1156
BmgBI CAC'GTC
Cuts at: 1318 2323 1318
Size: 1005 5089
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3043 3825 4783
Size: 416 53 83 399 24 1626 782 958
Cuts at: 4783 442
Size: 1753
Fragments arranged by size:
1753 1626 958 782 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6094
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2096 2117 2189 2255 2340 2783 3143 3236
Size: 857 21 72 66 85 443 360 93
Cuts at: 3236 4754 1239
Size: 1518 2579
Fragments arranged by size:
2579 1518 857 443 360 93 85 72
66 21
Bpu10I CC'TnA_GC
Cuts at: 3183 3183
Size: 6094
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2792 4219 5084 5382 2792
Size: 1427 865 298 3504
Fragments arranged by size:
3504 1427 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2270 2428 2444 2583 3022 4757 882
Size: 617 771 158 16 139 439 1735 2219
Fragments arranged by size:
2219 1735 771 617 439 158 139 16
BsaAI yAC'GTr
Cuts at: 493 1204 3885 493
Size: 711 2681 2702
Fragments arranged by size:
2702 2681 711
BsaBI GATnn'nnATC
Cuts at: 6088 6088
Size: 6094
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2178 2268 2725 2829
Size: 53 83 186 936 645 90 457 104
Cuts at: 2829 3063 3189 3901 4283 4918 275
Size: 234 126 712 382 635 1451
Fragments arranged by size:
1451 936 712 645 635 457 382 234
186 126 104 90 83 53
BsaWI w'CCGG_w
Cuts at: 4527 4925 5199 5346 5757 4527
Size: 398 274 147 411 4864
Fragments arranged by size:
4864 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2699 2729 1178
Size: 30 165 30 1296 30 4543
Fragments arranged by size:
4543 1296 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2054 1186
Size: 868 5226
BseYI C'CCAG_C
Cuts at: 2541 3192 5297 5793 2541
Size: 651 2105 496 2842
Fragments arranged by size:
2842 2105 651 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5521
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2549 2865 3544 4305 5333 1540
Size: 275 186 548 316 679 761 1028 2301
Fragments arranged by size:
2301 1028 761 679 548 316 275 186
BsiHKAI G_wGCw'C
Cuts at: 2706 4155 4240 5311 2706
Size: 1449 85 1071 3489
Fragments arranged by size:
3489 1449 1071 85
BsmI GAATG_Cn'
Cuts at: 3605 3698 3605
Size: 93 6001
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2706 3287 3413 4155 4240 5311 1391
Size: 399 916 581 126 742 85 1071 2174
Fragments arranged by size:
2174 1071 916 742 581 399 126 85
BspEI T'CCGG_A
Cuts at: 5757 5757
Size: 6094
BspHI T'CATG_A
Cuts at: 3983 5713 3983
Size: 1730 4364
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2346 2377 2718 2947 2963 2988 3332 3449
Size: 1502 31 341 229 16 25 344 117
Cuts at: 3449 844
Size: 3489
Fragments arranged by size:
3489 1502 344 341 229 117 31 25
16
BsrBI CCG'CTC
Cuts at: 1394 1823 2605 2997 3981 1394
Size: 429 782 392 984 3507
Fragments arranged by size:
3507 984 782 429 392
BsrDI GCAATG_nn'
Cuts at: 65 1248 4589 4763 65
Size: 1183 3341 174 1396
Fragments arranged by size:
3341 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2348 2618 2662 2721 2845 4738 4925
Size: 110 538 270 44 59 124 1893 187
Cuts at: 4925 1700
Size: 2869
Fragments arranged by size:
2869 1893 538 270 187 124 110 59
44
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6094
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6094
BssSI C'ACGA_G
Cuts at: 4154 5166 4154
Size: 1012 5082
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2710 794
Size: 1916 4178
BstBI TT'CG_AA
Cuts at: 4898 4898
Size: 6094
BstEII G'GTnAC_C
Cuts at: 1184 2105 2975 1184
Size: 921 870 4303
Fragments arranged by size:
4303 921 870
BstXI CCAn_nnnn'nTGG
Cuts at: 2059 3073 2059
Size: 1014 5080
BstYI r'GATC_y
Cuts at: 1972 2469 2583 3424 3791 4176 4193 5645 5731
Size: 497 114 841 367 385 17 1452 86
Cuts at: 5731 5743 6089 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 841 497 385 367 346 114
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2025 2151 513
Size: 552 939 21 126 4456
Fragments arranged by size:
4456 939 552 126 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5355
Fragments arranged by size:
5355 683 56
BtsI GCAGTG_nn'
Cuts at: 2894 3610 4404 4424 2894
Size: 716 794 20 4564
Fragments arranged by size:
4564 794 716 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6094
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6059
DraI TTT'AAA
Cuts at: 3398 3398
Size: 6094
DraIII CAC_nnn'GTG
Cuts at: 2900 2900
Size: 6094
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5101 809
Size: 507 3785 1802
Fragments arranged by size:
3785 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2061 2460 2529
Size: 54 1425 50 275 18 231 399 69
Cuts at: 2529 2546 2862 2940 2949 3006 3255 3541 4430
Size: 17 316 78 9 57 249 286 889
Cuts at: 4430 6059 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 399 316 286 275 249
231 78 69 57 54 50 43 18
17 9
EagI C'GGCC_G
Cuts at: 1537 1812 2546 2862 3541 1537
Size: 275 734 316 679 4090
Fragments arranged by size:
4090 734 679 316 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3381 4024 1353
Size: 2028 643 3423
Fragments arranged by size:
3423 2028 643
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2153 4681 5055 5201 387
Size: 1064 138 564 2528 374 146 1280
Fragments arranged by size:
2528 1280 1064 564 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2096 2117 2189 2255 2340 2380 2542
Size: 393 464 21 72 66 85 40 162
Cuts at: 2542 2783 3143 3236 4157 4754 5541 5809 1239
Size: 241 360 93 921 597 787 268 1524
Fragments arranged by size:
1524 921 787 597 464 393 360 268
241 162 93 85 72 66 40 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2114 2384 2477 3088 2114
Size: 270 93 611 5120
Fragments arranged by size:
5120 611 270 93
EcoO109I rG'GnC_Cy
Cuts at: 2080 2245 2335 2521 2839 3211 2080
Size: 165 90 186 318 372 4963
Fragments arranged by size:
4963 372 318 186 165 90
EcoRI G'AATT_C
Cuts at: 3403 3781 3403
Size: 378 5716
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2512 2512
Size: 6094
FspI TGC'GCA
Cuts at: 2087 2504 4600 2087
Size: 417 2096 3581
Fragments arranged by size:
3581 2096 417
FspAI rTGC'GCAy
Cuts at: 2504 2504
Size: 6094
HaeII r_GCGC'y
Cuts at: 1678 2728 3066 3144 5241 1678
Size: 1050 338 78 2097 2531
Fragments arranged by size:
2531 2097 1050 338 78
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2699 2731 2918 2950 2969
Size: 32 135 32 1127 32 187 32 19
Cuts at: 2969 3001 4735 4767 4809 4841 1373
Size: 32 1734 32 42 32 2626
Fragments arranged by size:
2626 1734 1127 187 135 42 32 32
32 32 32 32 32 19
HincII GTy'rAC
Cuts at: 669 1657 1938 3438 3684 669
Size: 988 281 1500 246 3079
Fragments arranged by size:
3079 1500 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6094
HpaI GTT'AAC
Cuts at: 3684 3684
Size: 6094
KasI G'GCGC_C
Cuts at: 2724 3062 2724
Size: 338 5756
KpnI G_GTAC'C
Cuts at: 3419 3419
Size: 6094
MfeI C'AATT_G
Cuts at: 3693 3693
Size: 6094
MluI A'CGCG_T
Cuts at: 5970 5970
Size: 6094
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2676 3081 5208 5392 1305
Size: 1371 405 2127 184 2007
Fragments arranged by size:
2127 2007 1371 405 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2063 2942 3008 3257 10
Size: 54 1425 343 231 879 66 249 2847
Fragments arranged by size:
2847 1425 879 343 249 231 66 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2057 2510 2687 3071 3101 4052 4411
Size: 648 891 453 177 384 30 951 359
Cuts at: 4411 4570 518
Size: 159 2042
Fragments arranged by size:
2042 951 891 648 453 384 359 177
159 30
MspA1I CmG'CkG
Cuts at: 1606 2436 2465 2545 2570 3366 3510 4187 5335
Size: 830 29 80 25 796 144 677 1148
Cuts at: 5335 5580 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 830 796 677 245 144 80
29 25
NaeI GCC'GGC
Cuts at: 2350 2620 2664 2350
Size: 270 44 5780
Fragments arranged by size:
5780 270 44
NarI GG'CG_CC
Cuts at: 2725 3063 2725
Size: 338 5756
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4603
Fragments arranged by size:
4603 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6094
NgoMIV G'CCGG_C
Cuts at: 2348 2618 2662 2348
Size: 270 44 5780
Fragments arranged by size:
5780 270 44
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6094
NotI GC'GGCC_GC
Cuts at: 2862 3541 2862
Size: 679 5415
NspI r_CATG'y
Cuts at: 1132 1229 3452 4997 1132
Size: 97 2223 1545 2229
Fragments arranged by size:
2229 2223 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2852 1348
Size: 1504 4590
PciI A'CATG_T
Cuts at: 4993 4993
Size: 6094
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6094
PfoI T'CCnGG_A
Cuts at: 2133 2133
Size: 6094
PmeI GTTT'AAAC
Cuts at: 3398 3398
Size: 6094
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4163 4195 5676 5708 4163
Size: 32 1481 32 4549
Fragments arranged by size:
4549 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2245 2335 2521 2245
Size: 90 186 5818
Fragments arranged by size:
5818 186 90
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6094
PsiI TTA'TAA
Cuts at: 3664 3664
Size: 6094
PstI C_TGCA'G
Cuts at: 830 2580 3329 3446 830
Size: 1750 749 117 3478
Fragments arranged by size:
3478 1750 749 117
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6094
PvuII CAG'CTG
Cuts at: 3366 3366
Size: 6094
SalI G'TCGA_C
Cuts at: 1936 3436 1936
Size: 1500 4594
SbfI CC_TGCA'GG
Cuts at: 2580 3329 3446 2580
Size: 749 117 5228
Fragments arranged by size:
5228 749 117
ScaI AGT'ACT
Cuts at: 4342 6054 4342
Size: 1712 4382
SfcI C'TryA_G
Cuts at: 826 1045 2576 3325 3442 4577 5258 5449 826
Size: 219 1531 749 117 1135 681 191 1471
Fragments arranged by size:
1531 1471 1135 749 681 219 191 117
SfiI GGCCn_nnn'nGGCC
Cuts at: 2948 2948
Size: 6094
SfoI GGC'GCC
Cuts at: 2726 3064 2726
Size: 338 5756
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6094
SgrAI Cr'CCGG_yG
Cuts at: 1700 2721 1700
Size: 1021 5073
SmaI CCC'GGG
Cuts at: 1872 3421 1872
Size: 1549 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2807 3347 4198 5099 5361 820
Size: 197 926 864 540 851 901 262 1553
Fragments arranged by size:
1553 926 901 864 851 540 262 197
SnaBI TAC'GTA
Cuts at: 493 3885 493
Size: 3392 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6094
SphI G_CATG'C
Cuts at: 1132 3452 1132
Size: 2320 3774
SspI AAT'ATT
Cuts at: 5 52 4018 5
Size: 47 3966 2081
Fragments arranged by size:
3966 2081 47
StuI AGG'CCT
Cuts at: 1517 2768 1517
Size: 1251 4843
StyI C'CwwG_G
Cuts at: 513 1065 2004 2064 2508 3533 513
Size: 552 939 60 444 1025 3074
Fragments arranged by size:
3074 1025 939 552 444 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2322 2555 2977 4121 4138 4291 4476
Size: 1255 742 233 422 1144 17 153 185
Cuts at: 4476 4921 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1144 742 445 422 233 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3351 4340 6052
Size: 274 80 33 51 1443 1374 989 1712
Cuts at: 6052 96
Size: 138
Fragments arranged by size:
1712 1443 1374 989 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6054 6086 6054
Size: 32 6062
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6094
XbaI T'CTAG_A
Cuts at: 3430 3430
Size: 6094
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2071 2897 2915 2071
Size: 826 18 5250
Fragments arranged by size:
5250 826 18
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6094
XmaI C'CCGG_G
Cuts at: 1870 3419 1870
Size: 1549 4545
XmnI GAAnn'nnTTC
Cuts at: 4223 4223
Size: 6094
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3902 276
Size: 53 83 186 936 2368 2468
Fragments arranged by size:
2468 2368 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FseI NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SrfI SwaI