Restriction map for
pFN21AB8835
(Circular) (Six-base) MAPSORT of: pFN21AB8835.seq Check: 1722 from: 1 to: 6082
pFN21AB8835 6082 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3892 278
Size: 53 83 186 936 2356 2468
Fragments arranged by size:
2468 2356 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3425 1937
Size: 1488 4594
Acc65I G'GTAC_C
Cuts at: 3403 3403
Size: 6082
AclI AA'CG_TT
Cuts at: 1563 4209 4582 1563
Size: 2646 373 3063
Fragments arranged by size:
3063 2646 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4145 5529 5797 1632
Size: 2513 1384 268 1917
Fragments arranged by size:
2513 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5885
AflIII A'CryG_T
Cuts at: 1203 2616 4981 5958 1203
Size: 1413 2365 977 1327
Fragments arranged by size:
2365 1413 1327 977
AgeI A'CCGG_T
Cuts at: 4913 4913
Size: 6082
AhdI GACnn_n'nnGTC
Cuts at: 4811 4811
Size: 6082
AleI CACnn'nnGTG
Cuts at: 1166 3166 1166
Size: 2000 4082
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6082
AlwNI CAG_nnn'CTG
Cuts at: 2191 3825 5397 2191
Size: 1634 1572 2876
Fragments arranged by size:
2876 1634 1572
ApaLI G'TGCA_C
Cuts at: 2235 3007 4139 5295 2235
Size: 772 1132 1156 3022
Fragments arranged by size:
3022 1156 1132 772
ApoI r'AATT_y
Cuts at: 1493 2285 3391 3617 3769 5878 1493
Size: 792 1106 226 152 2109 1697
Fragments arranged by size:
2109 1697 1106 792 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4636 160
Size: 872 3604 1606
Fragments arranged by size:
3604 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6082
AvaI C'yCGr_G
Cuts at: 1870 1943 2878 2916 3232 3407 1870
Size: 73 935 38 316 175 4545
Fragments arranged by size:
4545 935 316 175 73 38
BamHI G'GATC_C
Cuts at: 3412 3779 3412
Size: 367 5715
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2276 3300 3403 4858 618
Size: 325 217 627 489 1024 103 1455 1842
Fragments arranged by size:
1842 1455 1024 627 489 325 217 103
BanII G_rGCy'C
Cuts at: 1391 2391 3401 1391
Size: 1000 1010 4072
Fragments arranged by size:
4072 1010 1000
BbeI G_GCGC'C
Cuts at: 2280 2280
Size: 6082
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2574 3043 928
Size: 971 675 469 3967
Fragments arranged by size:
3967 971 675 469
BbvCI CC'TCA_GC
Cuts at: 2372 2372
Size: 6082
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4273 4307 1903
Size: 34 2336 34 3678
Fragments arranged by size:
3678 2336 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3974 5184 3974
Size: 1210 4872
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6082
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2921 3356 4693
Size: 107 122 71 631 605 1249 435 1337
Cuts at: 4693 136
Size: 1525
Fragments arranged by size:
1525 1337 1249 631 605 435 122 107
71
BglII A'GATC_T
Cuts at: 6077 6077
Size: 6082
BlpI GC'TnA_GC
Cuts at: 3499 3499
Size: 6082
Bme1580I G_kGCm'C
Cuts at: 1790 2239 3011 3020 3089 4143 5299 1790
Size: 449 772 9 69 1054 1156 2573
Fragments arranged by size:
2573 1156 1054 772 449 69 9
BmgBI CAC'GTC
Cuts at: 1318 2107 2617 3268 1318
Size: 789 510 651 4132
Fragments arranged by size:
4132 789 651 510
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3813 4771 442
Size: 416 53 83 399 24 2396 958 1753
Fragments arranged by size:
2396 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2430 1056
Size: 1374 4708
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2454 2570 2675 2703 3365 4742 1239
Size: 1215 116 105 28 662 1377 2579
Fragments arranged by size:
2579 1377 1215 662 116 105 28
Bpu10I CC'TnA_GC
Cuts at: 2372 2372
Size: 6082
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2114 3290 4207 5072 5370 2114
Size: 1176 917 865 298 2826
Fragments arranged by size:
2826 1176 917 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2224 4745 882
Size: 617 725 2521 2219
Fragments arranged by size:
2521 2219 725 617
BsaAI yAC'GTr
Cuts at: 493 1204 2347 3873 493
Size: 711 1143 1526 2702
Fragments arranged by size:
2702 1526 1143 711
BsaBI GATnn'nnATC
Cuts at: 6076 6076
Size: 6082
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2277 3889 4271 4906
Size: 53 83 186 936 744 1612 382 635
Cuts at: 4906 275
Size: 1451
Fragments arranged by size:
1612 1451 936 744 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4515 4913 5187 5334 5745 4515
Size: 398 274 147 411 4852
Fragments arranged by size:
4852 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2703 2733 3239 3269 1178
Size: 30 165 30 1300 30 506 30 3991
Fragments arranged by size:
3991 1300 506 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2105 2249 2453 2505 1186
Size: 919 144 204 52 4763
Fragments arranged by size:
4763 919 204 144 52
BseYI C'CCAG_C
Cuts at: 2201 2762 5285 5781 2201
Size: 561 2523 496 2502
Fragments arranged by size:
2523 2502 561 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3212 1163
Size: 573 1476 4033
Fragments arranged by size:
4033 1476 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3333 3532 4293 5321 1540
Size: 275 186 1332 199 761 1028 2301
Fragments arranged by size:
2301 1332 1028 761 275 199 186
BsiHKAI G_wGCw'C
Cuts at: 2239 2346 3011 4143 4228 5299 2239
Size: 107 665 1132 85 1071 3022
Fragments arranged by size:
3022 1132 1071 665 107 85
BsmI GAATG_Cn'
Cuts at: 2371 3593 3686 2371
Size: 1222 93 4767
Fragments arranged by size:
4767 1222 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2239 2346 2391 3011 3020 3089 3401
Size: 399 449 107 45 620 9 69 312
Cuts at: 3401 4143 4228 5299 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 620 449 399 312 107
85 69 45 9
BspEI T'CCGG_A
Cuts at: 5745 5745
Size: 6082
BspHI T'CATG_A
Cuts at: 3971 5701 3971
Size: 1730 4352
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2206 2340 3234 3437 844
Size: 1362 134 894 203 3489
Fragments arranged by size:
3489 1362 894 203 134
BsrBI CCG'CTC
Cuts at: 1394 1823 2095 3969 1394
Size: 429 272 1874 3507
Fragments arranged by size:
3507 1874 429 272
BsrDI GCAATG_nn'
Cuts at: 65 1248 4577 4751 65
Size: 1183 3329 174 1396
Fragments arranged by size:
3329 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2667 4726 4913 1700
Size: 110 857 2059 187 2869
Fragments arranged by size:
2869 2059 857 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6082
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6082
BssSI C'ACGA_G
Cuts at: 2048 3166 4142 5154 2048
Size: 1118 976 1012 2976
Fragments arranged by size:
2976 1118 1012 976
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3015 794
Size: 2221 3861
BstBI TT'CG_AA
Cuts at: 4886 4886
Size: 6082
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6082
BstYI r'GATC_y
Cuts at: 1972 3412 3779 4164 4181 5633 5719 5731 6077
Size: 1440 367 385 17 1452 86 12 346
Cuts at: 6077 1972
Size: 1977
Fragments arranged by size:
1977 1452 1440 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2718 2907 3327 513
Size: 552 939 714 189 420 3268
Fragments arranged by size:
3268 939 714 552 420 189
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5343
Fragments arranged by size:
5343 683 56
BtsI GCAGTG_nn'
Cuts at: 3598 4392 4412 3598
Size: 794 20 5268
Fragments arranged by size:
5268 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6082
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6047
DraI TTT'AAA
Cuts at: 3386 3386
Size: 6082
DraIII CAC_nnn'GTG
Cuts at: 2350 3015 2350
Size: 665 5417
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5089 809
Size: 507 3773 1802
Fragments arranged by size:
3773 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2044 2118 2157
Size: 54 1425 50 275 18 214 74 39
Cuts at: 2157 2247 2537 2725 2904 3330 3357 3529 4418
Size: 90 290 188 179 426 27 172 889
Cuts at: 4418 6047 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 426 290 275 214 188
179 172 90 74 54 50 43 39
27 18
EagI C'GGCC_G
Cuts at: 1537 1812 3330 3529 1537
Size: 275 1518 199 4090
Fragments arranged by size:
4090 1518 275 199
EarI CTCTTCn'nnn_
Cuts at: 1353 2586 4012 1353
Size: 1233 1426 3423
Fragments arranged by size:
3423 1426 1233
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4669 5043 5189 387
Size: 1064 138 3080 374 146 1280
Fragments arranged by size:
3080 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2454 2570 2675 2703 3365 4145 4742
Size: 393 822 116 105 28 662 780 597
Cuts at: 4742 5529 5797 1239
Size: 787 268 1524
Fragments arranged by size:
1524 822 787 780 662 597 393 268
116 105 28
EcoNI CCTnn'n_nnAGG
Cuts at: 2405 2405
Size: 6082
EcoO109I rG'GnC_Cy
Cuts at: 2478 2833 2957 2478
Size: 355 124 5603
Fragments arranged by size:
5603 355 124
EcoRI G'AATT_C
Cuts at: 2285 3391 3769 2285
Size: 1106 378 4598
Fragments arranged by size:
4598 1106 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2170 3067 2170
Size: 897 5185
FspI TGC'GCA
Cuts at: 4588 4588
Size: 6082
HaeII r_GCGC'y
Cuts at: 1678 2280 2451 5229 1678
Size: 602 171 2778 2531
Fragments arranged by size:
2778 2531 602 171
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2603 2635 4723 4755 4797
Size: 32 135 32 1031 32 2088 32 42
Cuts at: 4797 4829 1373
Size: 32 2626
Fragments arranged by size:
2626 2088 1031 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2308 3426 3672 669
Size: 988 281 370 1118 246 3079
Fragments arranged by size:
3079 1118 988 370 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6082
HpaI GTT'AAC
Cuts at: 3672 3672
Size: 6082
KasI G'GCGC_C
Cuts at: 2276 2276
Size: 6082
KpnI G_GTAC'C
Cuts at: 3407 3407
Size: 6082
MfeI C'AATT_G
Cuts at: 3681 3681
Size: 6082
MluI A'CGCG_T
Cuts at: 5958 5958
Size: 6082
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2793 5196 5380 1305
Size: 1488 2403 184 2007
Fragments arranged by size:
2403 2007 1488 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2727 2906 10
Size: 54 1425 343 895 179 3186
Fragments arranged by size:
3186 1425 895 343 179 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3080 3140 3150 3166 4040 4399 4558
Size: 648 1914 60 10 16 874 359 159
Cuts at: 4558 518
Size: 2042
Fragments arranged by size:
2042 1914 874 648 359 159 60 16
10
MspA1I CmG'CkG
Cuts at: 1606 2038 2900 3252 3498 4175 5323 5568 1606
Size: 432 862 352 246 677 1148 245 2120
Fragments arranged by size:
2120 1148 862 677 432 352 246 245
NarI GG'CG_CC
Cuts at: 2277 2277
Size: 6082
NcoI C'CATG_G
Cuts at: 513 1065 2004 2907 513
Size: 552 939 903 3688
Fragments arranged by size:
3688 939 903 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6082
NheI G'CTAG_C
Cuts at: 1052 2426 1052
Size: 1374 4708
NotI GC'GGCC_GC
Cuts at: 3529 3529
Size: 6082
NspI r_CATG'y
Cuts at: 1132 1229 3061 3440 4985 1132
Size: 97 1832 379 1545 2229
Fragments arranged by size:
2229 1832 1545 379 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6082
PciI A'CATG_T
Cuts at: 4981 4981
Size: 6082
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3037 1108
Size: 1929 4153
PfoI T'CCnGG_A
Cuts at: 2848 2848
Size: 6082
PmeI GTTT'AAAC
Cuts at: 3386 3386
Size: 6082
PmlI CAC'GTG
Cuts at: 2347 2347
Size: 6082
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2081 2113 2189 2221 4151 4183 5664 5696 2081
Size: 32 76 32 1930 32 1481 32 2467
Fragments arranged by size:
2467 1930 1481 76 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2478 2957 2478
Size: 479 5603
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6082
PsiI TTA'TAA
Cuts at: 3652 3652
Size: 6082
PstI C_TGCA'G
Cuts at: 830 2217 2337 3434 830
Size: 1387 120 1097 3478
Fragments arranged by size:
3478 1387 1097 120
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6082
PvuII CAG'CTG
Cuts at: 2038 2038
Size: 6082
SalI G'TCGA_C
Cuts at: 1936 3424 1936
Size: 1488 4594
SanDI GG'GwC_CC
Cuts at: 2957 2957
Size: 6082
SbfI CC_TGCA'GG
Cuts at: 3434 3434
Size: 6082
ScaI AGT'ACT
Cuts at: 4330 6042 4330
Size: 1712 4370
SfcI C'TryA_G
Cuts at: 826 1045 2213 2333 2769 2803 3430 4565 5246
Size: 219 1168 120 436 34 627 1135 681
Cuts at: 5246 5437 826
Size: 191 1471
Fragments arranged by size:
1471 1168 1135 681 627 436 219 191
120 34
SfiI GGCCn_nnn'nGGCC
Cuts at: 3356 3356
Size: 6082
SfoI GGC'GCC
Cuts at: 2278 2278
Size: 6082
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6082
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6082
SmaI CCC'GGG
Cuts at: 1872 2918 3409 1872
Size: 1046 491 4545
Fragments arranged by size:
4545 1046 491
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2129 3305 4186 5087 5349 820
Size: 197 926 186 1176 881 901 262 1553
Fragments arranged by size:
1553 1176 926 901 881 262 197 186
SnaBI TAC'GTA
Cuts at: 493 3873 493
Size: 3380 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6082
SphI G_CATG'C
Cuts at: 1132 3440 1132
Size: 2308 3774
SspI AAT'ATT
Cuts at: 5 52 4006 5
Size: 47 3954 2081
Fragments arranged by size:
3954 2081 47
StuI AGG'CCT
Cuts at: 1517 2400 2688 2798 1517
Size: 883 288 110 4801
Fragments arranged by size:
4801 883 288 110
StyI C'CwwG_G
Cuts at: 513 1065 2004 2280 2731 2907 3521 513
Size: 552 939 276 451 176 614 3074
Fragments arranged by size:
3074 939 614 552 451 276 176
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2711 4109 4126 4279 4464 4909 325
Size: 1255 1131 1398 17 153 185 445 1498
Fragments arranged by size:
1498 1398 1255 1131 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4328 6040 96
Size: 274 80 33 51 1443 2351 1712 138
Fragments arranged by size:
2351 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2703 2735 6042 6074 2703
Size: 32 3307 32 2711
Fragments arranged by size:
3307 2711 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2303 1316
Size: 987 5095
XbaI T'CTAG_A
Cuts at: 3418 3418
Size: 6082
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2881 2881
Size: 6082
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6082
XmaI C'CCGG_G
Cuts at: 1870 2916 3407 1870
Size: 1046 491 4545
Fragments arranged by size:
4545 1046 491
XmnI GAAnn'nnTTC
Cuts at: 4211 4211
Size: 6082
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3890 276
Size: 53 83 186 936 2356 2468
Fragments arranged by size:
2468 2356 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV
FseI FspAI NaeI NgoMIV NruI NsiI PacI PspOMI
PspXI PsrI RsrII SacI SacII SapI SexAI SrfI
SwaI