Restriction map for
pFN21AB8832
(Circular) (Six-base) MAPSORT of: pFN21AB8832.seq Check: 1796 from: 1 to: 6061
pFN21AB8832 6061 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2664 2664
Size: 6061
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3871 278
Size: 53 83 186 936 2335 2468
Fragments arranged by size:
2468 2335 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3404 1937
Size: 1467 4594
Acc65I G'GTAC_C
Cuts at: 3382 3382
Size: 6061
AclI AA'CG_TT
Cuts at: 1563 4188 4561 1563
Size: 2625 373 3063
Fragments arranged by size:
3063 2625 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2779 2947 3115 3199 4124 5508 5776 1632
Size: 1147 168 168 84 925 1384 268 1917
Fragments arranged by size:
1917 1384 1147 925 268 168 168 84
AfeI AGC'GCT
Cuts at: 2153 2888 2153
Size: 735 5326
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5864
AflIII A'CryG_T
Cuts at: 1203 2559 4960 5937 1203
Size: 1356 2401 977 1327
Fragments arranged by size:
2401 1356 1327 977
AgeI A'CCGG_T
Cuts at: 4892 4892
Size: 6061
AhdI GACnn_n'nnGTC
Cuts at: 2585 4790 2585
Size: 2205 3856
AleI CACnn'nnGTG
Cuts at: 1166 2115 2604 2729 2854 3053 1166
Size: 949 489 125 125 199 4174
Fragments arranged by size:
4174 949 489 199 125 125
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6061
AlwNI CAG_nnn'CTG
Cuts at: 2371 3804 5376 2371
Size: 1433 1572 3056
Fragments arranged by size:
3056 1572 1433
ApaI G_GGCC'C
Cuts at: 3253 3253
Size: 6061
ApaLI G'TGCA_C
Cuts at: 2157 2903 4118 5274 2157
Size: 746 1215 1156 2944
Fragments arranged by size:
2944 1215 1156 746
ApoI r'AATT_y
Cuts at: 1493 3370 3596 3748 5857 1493
Size: 1877 226 152 2109 1697
Fragments arranged by size:
2109 1877 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4615 160
Size: 872 3583 1606
Fragments arranged by size:
3583 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6061
AvaI C'yCGr_G
Cuts at: 1870 1943 2668 2945 3113 3386 1870
Size: 73 725 277 168 273 4545
Fragments arranged by size:
4545 725 277 273 168 73
BamHI G'GATC_C
Cuts at: 3391 3758 3391
Size: 367 5694
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2358 2528 3382 4837 618
Size: 325 217 627 571 170 854 1455 1842
Fragments arranged by size:
1842 1455 854 627 571 325 217 170
BanII G_rGCy'C
Cuts at: 1391 2110 2573 2639 3253 3380 1391
Size: 719 463 66 614 127 4072
Fragments arranged by size:
4072 719 614 463 127 66
BbeI G_GCGC'C
Cuts at: 2362 2532 2362
Size: 170 5891
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5090
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2860 2894 3292 3326 4252 4286 1903
Size: 34 923 34 398 34 926 34 3678
Fragments arranged by size:
3678 926 923 398 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3953 5163 3953
Size: 1210 4851
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6061
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2250 2380 2838
Size: 107 122 71 631 605 578 130 458
Cuts at: 2838 4672 136
Size: 1834 1525
Fragments arranged by size:
1834 1525 631 605 578 458 130 122
107 71
BglII A'GATC_T
Cuts at: 6056 6056
Size: 6061
BlpI GC'TnA_GC
Cuts at: 3478 3478
Size: 6061
Bme1580I G_kGCm'C
Cuts at: 1790 2161 2510 2693 2907 2945 3253 3305 4122
Size: 371 349 183 214 38 308 52 817
Cuts at: 4122 5278 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 817 371 349 308 214 183
52 38
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6061
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3792 4750 442
Size: 416 53 83 399 24 2375 958 1753
Fragments arranged by size:
2375 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6061
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2735 4721 1239
Size: 1496 1986 2579
Fragments arranged by size:
2579 1986 1496
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4186 5051 5349 4186
Size: 865 298 4898
Fragments arranged by size:
4898 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4724 882
Size: 617 3225 2219
Fragments arranged by size:
3225 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2560 3051 3852 493
Size: 711 1356 491 801 2702
Fragments arranged by size:
2702 1356 801 711 491
BsaBI GATnn'nnATC
Cuts at: 6055 6055
Size: 6061
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2359 2529 2595 3868
Size: 53 83 186 936 826 170 66 1273
Cuts at: 3868 4250 4885 275
Size: 382 635 1451
Fragments arranged by size:
1451 1273 936 826 635 382 186 170
83 66 53
BsaWI w'CCGG_w
Cuts at: 4494 4892 5166 5313 5724 4494
Size: 398 274 147 411 4831
Fragments arranged by size:
4831 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5836
Fragments arranged by size:
5836 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6061
BseYI C'CCAG_C
Cuts at: 5264 5760 5264
Size: 496 5565
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2071 3139 3140 1163
Size: 573 335 1068 1 4084
Fragments arranged by size:
4084 1068 573 335 1
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2125 2254 2320 2956 2971 3511
Size: 275 186 124 129 66 636 15 540
Cuts at: 3511 4272 5300 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1028 761 636 540 275 186 129
124 66 15
BsiHKAI G_wGCw'C
Cuts at: 2110 2161 2639 2907 4122 4207 5278 2110
Size: 51 478 268 1215 85 1071 2893
Fragments arranged by size:
2893 1215 1071 478 268 85 51
BsiWI C'GTAC_G
Cuts at: 2766 2766
Size: 6061
BsmI GAATG_Cn'
Cuts at: 2147 3572 3665 2147
Size: 1425 93 4543
Fragments arranged by size:
4543 1425 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2110 2161 2510 2573 2639 2693 2907
Size: 399 320 51 349 63 66 54 214
Cuts at: 2907 2945 3253 3305 3380 4122 4207 5278 1391
Size: 38 308 52 75 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 349 320 308 214
85 75 66 63 54 52 51 38
BspEI T'CCGG_A
Cuts at: 5724 5724
Size: 6061
BspHI T'CATG_A
Cuts at: 3950 5680 3950
Size: 1730 4331
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2664 3416 844
Size: 1820 752 3489
Fragments arranged by size:
3489 1820 752
BsrBI CCG'CTC
Cuts at: 1394 1823 2380 2845 2973 3228 3948 1394
Size: 429 557 465 128 255 720 3507
Fragments arranged by size:
3507 720 557 465 429 255 128
BsrDI GCAATG_nn'
Cuts at: 65 1248 4556 4730 65
Size: 1183 3308 174 1396
Fragments arranged by size:
3308 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2280 2319 2355 2991 3089 3325 4705
Size: 110 470 39 36 636 98 236 1380
Cuts at: 4705 4892 1700
Size: 187 2869
Fragments arranged by size:
2869 1380 636 470 236 187 110 98
39 36
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6061
BssHII G'CGCG_C
Cuts at: 1925 2424 2499 2549 3259 1925
Size: 499 75 50 710 4727
Fragments arranged by size:
4727 710 499 75 50
BssSI C'ACGA_G
Cuts at: 2047 4121 5133 2047
Size: 2074 1012 2975
Fragments arranged by size:
2975 2074 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2165 2226 794
Size: 1371 61 4629
Fragments arranged by size:
4629 1371 61
BstBI TT'CG_AA
Cuts at: 4865 4865
Size: 6061
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6061
BstXI CCAn_nnnn'nTGG
Cuts at: 2606 2606
Size: 6061
BstYI r'GATC_y
Cuts at: 1972 3391 3758 4143 4160 5612 5698 5710 6056
Size: 1419 367 385 17 1452 86 12 346
Cuts at: 6056 1972
Size: 1977
Fragments arranged by size:
1977 1452 1419 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5509
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2302 487
Size: 683 56 1076 4246
Fragments arranged by size:
4246 1076 683 56
BtsI GCAGTG_nn'
Cuts at: 2031 2227 2510 3152 3303 3577 4371 4391 2031
Size: 196 283 642 151 274 794 20 3701
Fragments arranged by size:
3701 794 642 283 274 196 151 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6061
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6026
DraI TTT'AAA
Cuts at: 3365 3365
Size: 6061
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5068 809
Size: 507 3752 1802
Fragments arranged by size:
3752 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2122 2200 2251
Size: 54 1425 50 275 18 292 78 51
Cuts at: 2251 2317 2846 2953 3091 3182 3508 4397 6026
Size: 66 529 107 138 91 326 889 1629
Cuts at: 6026 8
Size: 43
Fragments arranged by size:
1629 1425 889 529 326 292 275 138
107 91 78 66 54 51 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2122 2251 2317 2953 3508 1537
Size: 275 310 129 66 636 555 4090
Fragments arranged by size:
4090 636 555 310 275 129 66
EarI CTCTTCn'nnn_
Cuts at: 1353 2438 3991 1353
Size: 1085 1553 3423
Fragments arranged by size:
3423 1553 1085
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2231 4648 5022 5168 387
Size: 1064 138 642 2417 374 146 1280
Fragments arranged by size:
2417 1280 1064 642 374 146 138
EcoICRI GAG'CTC
Cuts at: 2108 2637 2108
Size: 529 5532
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2735 2779 2947 3115 3199 4124 4721
Size: 393 1103 44 168 168 84 925 597
Cuts at: 4721 5508 5776 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1103 925 787 597 393 268 168
168 84 44
EcoRI G'AATT_C
Cuts at: 3370 3748 3370
Size: 378 5683
FspI TGC'GCA
Cuts at: 3196 4567 3196
Size: 1371 4690
HaeII r_GCGC'y
Cuts at: 1678 2155 2326 2345 2362 2532 2724 2890 5208
Size: 477 171 19 17 170 192 166 2318
Cuts at: 5208 1678
Size: 2531
Fragments arranged by size:
2531 2318 477 192 171 170 166 19
17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2571 2603 4702 4734 4776
Size: 32 135 32 999 32 2099 32 42
Cuts at: 4776 4808 1373
Size: 32 2626
Fragments arranged by size:
2626 2099 999 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3405 3651 669
Size: 988 281 1467 246 3079
Fragments arranged by size:
3079 1467 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2236 3146 748
Size: 1488 910 3663
Fragments arranged by size:
3663 1488 910
HpaI GTT'AAC
Cuts at: 3651 3651
Size: 6061
KasI G'GCGC_C
Cuts at: 2358 2528 2358
Size: 170 5891
KpnI G_GTAC'C
Cuts at: 3386 3386
Size: 6061
MfeI C'AATT_G
Cuts at: 3660 3660
Size: 6061
MluI A'CGCG_T
Cuts at: 5937 5937
Size: 6061
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2916 5175 5359 1305
Size: 1611 2259 184 2007
Fragments arranged by size:
2259 2007 1611 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2202 10
Size: 54 1425 343 370 3869
Fragments arranged by size:
3869 1425 370 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2115 2558 2604 2729 2854 3053 3106
Size: 648 949 443 46 125 125 199 53
Cuts at: 3106 4019 4378 4537 518
Size: 913 359 159 2042
Fragments arranged by size:
2042 949 913 648 443 359 199 159
125 125 53 46
MspA1I CmG'CkG
Cuts at: 1606 2033 2250 2391 2398 2496 3024 3188 3247
Size: 427 217 141 7 98 528 164 59
Cuts at: 3247 3272 3477 4154 5302 5547 1606
Size: 25 205 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 528 427 245 217 205
164 141 98 59 25 7
NaeI GCC'GGC
Cuts at: 2321 3091 3327 2321
Size: 770 236 5055
Fragments arranged by size:
5055 770 236
NarI GG'CG_CC
Cuts at: 2359 2529 2359
Size: 170 5891
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5509
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6061
NgoMIV G'CCGG_C
Cuts at: 2319 3089 3325 2319
Size: 770 236 5055
Fragments arranged by size:
5055 770 236
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6061
NotI GC'GGCC_GC
Cuts at: 2953 3508 2953
Size: 555 5506
NspI r_CATG'y
Cuts at: 1132 1229 2198 2316 2775 2855 3419 4964 1132
Size: 97 969 118 459 80 564 1545 2229
Fragments arranged by size:
2229 1545 969 564 459 118 97 80
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6061
PciI A'CATG_T
Cuts at: 4960 4960
Size: 6061
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6061
PfoI T'CCnGG_A
Cuts at: 2564 2712 2564
Size: 148 5913
PmeI GTTT'AAAC
Cuts at: 3365 3365
Size: 6061
PmlI CAC'GTG
Cuts at: 2560 3051 2560
Size: 491 5570
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4130 4162 5643 5675 4130
Size: 32 1481 32 4516
Fragments arranged by size:
4516 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6061
PsiI TTA'TAA
Cuts at: 3631 3631
Size: 6061
PspOMI G'GGCC_C
Cuts at: 3249 3249
Size: 6061
PstI C_TGCA'G
Cuts at: 830 2222 2661 3413 830
Size: 1392 439 752 3478
Fragments arranged by size:
3478 1392 752 439
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6061
SacI G_AGCT'C
Cuts at: 2110 2639 2110
Size: 529 5532
SalI G'TCGA_C
Cuts at: 1936 3403 1936
Size: 1467 4594
SapI GCTCTTCn'nnn_
Cuts at: 2438 2438
Size: 6061
SbfI CC_TGCA'GG
Cuts at: 3413 3413
Size: 6061
ScaI AGT'ACT
Cuts at: 4309 6021 4309
Size: 1712 4349
SexAI A'CCwGG_T
Cuts at: 2895 2895
Size: 6061
SfcI C'TryA_G
Cuts at: 826 1045 2077 2218 2657 3409 4544 5225 5416
Size: 219 1032 141 439 752 1135 681 191
Cuts at: 5416 826
Size: 1471
Fragments arranged by size:
1471 1135 1032 752 681 439 219 191
141
SfoI GGC'GCC
Cuts at: 2360 2530 2360
Size: 170 5891
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6061
SgrAI Cr'CCGG_yG
Cuts at: 1700 2355 2991 1700
Size: 655 636 4770
Fragments arranged by size:
4770 655 636
SmaI CCC'GGG
Cuts at: 1872 3388 1872
Size: 1516 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4165 5066 5328 820
Size: 197 926 2222 901 262 1553
Fragments arranged by size:
2222 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3852 493
Size: 3359 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6061
SphI G_CATG'C
Cuts at: 1132 2198 3419 1132
Size: 1066 1221 3774
Fragments arranged by size:
3774 1221 1066
SspI AAT'ATT
Cuts at: 5 52 3985 5
Size: 47 3933 2081
Fragments arranged by size:
3933 2081 47
StuI AGG'CCT
Cuts at: 1517 3128 1517
Size: 1611 4450
StyI C'CwwG_G
Cuts at: 513 1065 2457 3500 513
Size: 552 1392 1043 3074
Fragments arranged by size:
3074 1392 1043 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4088 4105 4258 4443 4888 325
Size: 1255 2508 17 153 185 445 1498
Fragments arranged by size:
2508 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4307 6019 96
Size: 274 80 33 51 1443 2330 1712 138
Fragments arranged by size:
2330 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6021 6053 6021
Size: 32 6029
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6061
XbaI T'CTAG_A
Cuts at: 3397 3397
Size: 6061
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6061
XmaI C'CCGG_G
Cuts at: 1870 3386 1870
Size: 1516 4545
XmnI GAAnn'nnTTC
Cuts at: 2681 2933 4190 2681
Size: 252 1257 4552
Fragments arranged by size:
4552 1257 252
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3869 276
Size: 53 83 186 936 2335 2468
Fragments arranged by size:
2468 2335 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI
PvuI SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I
BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI
FseI FspAI NruI NsiI PacI PpuMI PspXI PsrI
PvuII RsrII SacII SanDI SfiI SrfI SwaI XcmI