Restriction map for
pFN21AB8830
(Circular) (Six-base) MAPSORT of: pFN21AB8830.seq Check: 2062 from: 1 to: 6025
pFN21AB8830 6025 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3835 278
Size: 53 83 186 936 2299 2468
Fragments arranged by size:
2468 2299 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3368 1937
Size: 1431 4594
Acc65I G'GTAC_C
Cuts at: 2921 3346 2921
Size: 425 5600
AclI AA'CG_TT
Cuts at: 1563 4152 4525 1563
Size: 2589 373 3063
Fragments arranged by size:
3063 2589 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2328 2825 3281 4088 5472 5740 1632
Size: 696 497 456 807 1384 268 1917
Fragments arranged by size:
1917 1384 807 696 497 456 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5828
AflIII A'CryG_T
Cuts at: 1203 4924 5901 1203
Size: 3721 977 1327
Fragments arranged by size:
3721 1327 977
AgeI A'CCGG_T
Cuts at: 4856 4856
Size: 6025
AhdI GACnn_n'nnGTC
Cuts at: 4754 4754
Size: 6025
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6025
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6025
AlwNI CAG_nnn'CTG
Cuts at: 2150 2287 3768 5340 2150
Size: 137 1481 1572 2835
Fragments arranged by size:
2835 1572 1481 137
ApaLI G'TGCA_C
Cuts at: 4082 5238 4082
Size: 1156 4869
ApoI r'AATT_y
Cuts at: 1493 2367 2467 2703 3334 3560 3712 5821 1493
Size: 874 100 236 631 226 152 2109 1697
Fragments arranged by size:
2109 1697 874 631 236 226 152 100
AseI AT'TA_AT
Cuts at: 160 1032 4579 160
Size: 872 3547 1606
Fragments arranged by size:
3547 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6025
AvaI C'yCGr_G
Cuts at: 1870 1943 3350 1870
Size: 73 1407 4545
Fragments arranged by size:
4545 1407 73
BamHI G'GATC_C
Cuts at: 3355 3722 3355
Size: 367 5658
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2921 3346 4801 618
Size: 325 217 627 1134 425 1455 1842
Fragments arranged by size:
1842 1455 1134 627 425 325 217
BanII G_rGCy'C
Cuts at: 1391 3084 3344 1391
Size: 1693 260 4072
Fragments arranged by size:
4072 1693 260
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2958 3049 928
Size: 971 1059 91 3904
Fragments arranged by size:
3904 1059 971 91
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4216 4250 1903
Size: 34 2279 34 3678
Fragments arranged by size:
3678 2279 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3917 5127 3917
Size: 1210 4815
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6025
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4636 136
Size: 107 122 71 631 605 2964 1525
Fragments arranged by size:
2964 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6020 6020
Size: 6025
BlpI GC'TnA_GC
Cuts at: 3442 3442
Size: 6025
Bme1580I G_kGCm'C
Cuts at: 1790 4086 5242 1790
Size: 2296 1156 2573
Fragments arranged by size:
2573 2296 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6025
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2875 3087 3756
Size: 416 53 83 399 24 1458 212 669
Cuts at: 3756 4714 442
Size: 958 1753
Fragments arranged by size:
1753 1458 958 669 416 399 212 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6025
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4685 1239
Size: 3446 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4150 5015 5313 4150
Size: 865 298 4862
Fragments arranged by size:
4862 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4688 882
Size: 617 3189 2219
Fragments arranged by size:
3189 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3816 493
Size: 711 2612 2702
Fragments arranged by size:
2702 2612 711
BsaBI GATnn'nnATC
Cuts at: 6019 6019
Size: 6025
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3832 4214 4849 275
Size: 53 83 186 936 2299 382 635 1451
Fragments arranged by size:
2299 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4458 4856 5130 5277 5688 4458
Size: 398 274 147 411 4795
Fragments arranged by size:
4795 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2056 2086 2820 2850 1178
Size: 30 165 30 653 30 734 30 4353
Fragments arranged by size:
4353 734 653 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6025
BseYI C'CCAG_C
Cuts at: 5228 5724 5228
Size: 496 5529
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5452
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3475 4236 5264 1540
Size: 275 186 1474 761 1028 2301
Fragments arranged by size:
2301 1474 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4086 4171 5242 4086
Size: 85 1071 4869
Fragments arranged by size:
4869 1071 85
BsmI GAATG_Cn'
Cuts at: 3536 3629 3536
Size: 93 5932
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3084 3344 4086 4171 5242 1391
Size: 399 1294 260 742 85 1071 2174
Fragments arranged by size:
2174 1294 1071 742 399 260 85
BspEI T'CCGG_A
Cuts at: 5688 5688
Size: 6025
BspHI T'CATG_A
Cuts at: 3914 5644 3914
Size: 1730 4295
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3380 844
Size: 2536 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3912 1394
Size: 429 2089 3507
Fragments arranged by size:
3507 2089 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3326 4520 4694 65
Size: 1183 2078 1194 174 1396
Fragments arranged by size:
2078 1396 1194 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4669 4856 1700
Size: 110 2859 187 2869
Fragments arranged by size:
2869 2859 187 110
BsrGI T'GTAC_A
Cuts at: 96 3203 96
Size: 3107 2918
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6025
BssSI C'ACGA_G
Cuts at: 4085 5097 4085
Size: 1012 5013
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6025
BstBI TT'CG_AA
Cuts at: 4829 4829
Size: 6025
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6025
BstXI CCAn_nnnn'nTGG
Cuts at: 2230 2865 2230
Size: 635 5390
BstYI r'GATC_y
Cuts at: 1972 3355 3722 4107 4124 5576 5662 5674 6020
Size: 1383 367 385 17 1452 86 12 346
Cuts at: 6020 1972
Size: 1977
Fragments arranged by size:
1977 1452 1383 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2298 513
Size: 552 939 294 4240
Fragments arranged by size:
4240 939 552 294
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5286
Fragments arranged by size:
5286 683 56
BtsI GCAGTG_nn'
Cuts at: 2312 2454 3541 4335 4355 2312
Size: 142 1087 794 20 3982
Fragments arranged by size:
3982 1087 794 142 20
ClaI AT'CG_AT
Cuts at: 1554 2257 3222 1554
Size: 703 965 4357
Fragments arranged by size:
4357 965 703
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5990
DraI TTT'AAA
Cuts at: 3329 3329
Size: 6025
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2985 3053 5032 809
Size: 507 1669 68 1979 1802
Fragments arranged by size:
1979 1802 1669 507 68
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3472 4361 5990
Size: 54 1425 50 275 18 1642 889 1629
Cuts at: 5990 8
Size: 43
Fragments arranged by size:
1642 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3472 1537
Size: 275 1660 4090
Fragments arranged by size:
4090 1660 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2347 3000 3955 1353
Size: 994 653 955 3423
Fragments arranged by size:
3423 994 955 653
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4612 4986 5132 387
Size: 1064 138 3023 374 146 1280
Fragments arranged by size:
3023 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2328 2825 3281 4088 4685 5472 5740
Size: 393 696 497 456 807 597 787 268
Cuts at: 5740 1239
Size: 1524
Fragments arranged by size:
1524 807 787 696 597 497 456 393
268
EcoO109I rG'GnC_Cy
Cuts at: 2627 3100 2627
Size: 473 5552
EcoRI G'AATT_C
Cuts at: 3334 3712 3334
Size: 378 5647
FspI TGC'GCA
Cuts at: 4531 4531
Size: 6025
HaeII r_GCGC'y
Cuts at: 1678 3111 5172 1678
Size: 1433 2061 2531
Fragments arranged by size:
2531 2061 1433
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2509 2541 4666 4698 4740
Size: 32 135 32 937 32 2125 32 42
Cuts at: 4740 4772 1373
Size: 32 2626
Fragments arranged by size:
2626 2125 937 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3369 3615 669
Size: 988 281 1431 246 3079
Fragments arranged by size:
3079 1431 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6025
HpaI GTT'AAC
Cuts at: 3615 3615
Size: 6025
KpnI G_GTAC'C
Cuts at: 2925 3350 2925
Size: 425 5600
MfeI C'AATT_G
Cuts at: 3624 3624
Size: 6025
MluI A'CGCG_T
Cuts at: 5901 5901
Size: 6025
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2042 2211 2757 5139 5323 1305
Size: 737 169 546 2382 184 2007
Fragments arranged by size:
2382 2007 737 546 184 169
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4203
Fragments arranged by size:
4203 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2247 2376 2577 3983 4342 4501 518
Size: 648 1081 129 201 1406 359 159 2042
Fragments arranged by size:
2042 1406 1081 648 359 201 159 129
MspA1I CmG'CkG
Cuts at: 1606 2214 3441 4118 5266 5511 1606
Size: 608 1227 677 1148 245 2120
Fragments arranged by size:
2120 1227 1148 677 608 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4534
Fragments arranged by size:
4534 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6025
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6025
NotI GC'GGCC_GC
Cuts at: 3472 3472
Size: 6025
NspI r_CATG'y
Cuts at: 1132 1229 3383 4928 1132
Size: 97 2154 1545 2229
Fragments arranged by size:
2229 2154 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6025
PciI A'CATG_T
Cuts at: 4924 4924
Size: 6025
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6025
PmeI GTTT'AAAC
Cuts at: 3329 3329
Size: 6025
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4094 4126 5607 5639 4094
Size: 32 1481 32 4480
Fragments arranged by size:
4480 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2627 3100 2627
Size: 473 5552
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6025
PsiI TTA'TAA
Cuts at: 3096 3276 3595 3096
Size: 180 319 5526
Fragments arranged by size:
5526 319 180
PstI C_TGCA'G
Cuts at: 830 2775 3174 3377 830
Size: 1945 399 203 3478
Fragments arranged by size:
3478 1945 399 203
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6025
PvuII CAG'CTG
Cuts at: 2214 2214
Size: 6025
SalI G'TCGA_C
Cuts at: 1936 3367 1936
Size: 1431 4594
SbfI CC_TGCA'GG
Cuts at: 3377 3377
Size: 6025
ScaI AGT'ACT
Cuts at: 3294 4273 5985 3294
Size: 979 1712 3334
Fragments arranged by size:
3334 1712 979
SexAI A'CCwGG_T
Cuts at: 2652 2652
Size: 6025
SfcI C'TryA_G
Cuts at: 826 1045 2771 2983 3170 3373 4508 5189 5380
Size: 219 1726 212 187 203 1135 681 191
Cuts at: 5380 826
Size: 1471
Fragments arranged by size:
1726 1471 1135 681 219 212 203 191
187
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6025
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6025
SmaI CCC'GGG
Cuts at: 1872 3352 1872
Size: 1480 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4129 5030 5292 820
Size: 197 926 2186 901 262 1553
Fragments arranged by size:
2186 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3816 493
Size: 3323 2702
SpeI A'CTAG_T
Cuts at: 152 2057 152
Size: 1905 4120
SphI G_CATG'C
Cuts at: 1132 3383 1132
Size: 2251 3774
SspI AAT'ATT
Cuts at: 5 52 2026 3949 5
Size: 47 1974 1923 2081
Fragments arranged by size:
2081 1974 1923 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6025
StyI C'CwwG_G
Cuts at: 513 1065 2004 3464 513
Size: 552 939 1460 3074
Fragments arranged by size:
3074 1460 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4052 4069 4222 4407 4852 325
Size: 1255 2472 17 153 185 445 1498
Fragments arranged by size:
2472 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3203 3292 4271
Size: 274 80 33 51 1443 1226 89 979
Cuts at: 4271 5983 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1226 979 274 138 89 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2056 2088 5985 6017 2056
Size: 32 3897 32 2064
Fragments arranged by size:
3897 2064 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6025
XbaI T'CTAG_A
Cuts at: 3361 3361
Size: 6025
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2752 2752
Size: 6025
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6025
XmaI C'CCGG_G
Cuts at: 1870 3350 1870
Size: 1480 4545
XmnI GAAnn'nnTTC
Cuts at: 3129 4154 3129
Size: 1025 5000
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3833 276
Size: 53 83 186 936 2299 2468
Fragments arranged by size:
2468 2299 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SfiI SfoI SrfI SwaI