Restriction map for
pFN21AB8828
(Circular) (Six-base) MAPSORT of: pFN21AB8828.seq Check: 2377 from: 1 to: 6007
pFN21AB8828 6007 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2487 2487
Size: 6007
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2563 3817 278
Size: 53 83 186 936 1027 1254 2468
Fragments arranged by size:
2468 1254 1027 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3350 1937
Size: 1413 4594
Acc65I G'GTAC_C
Cuts at: 2033 3328 2033
Size: 1295 4712
AclI AA'CG_TT
Cuts at: 1563 4134 4507 1563
Size: 2571 373 3063
Fragments arranged by size:
3063 2571 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4070 5454 5722 1632
Size: 2438 1384 268 1917
Fragments arranged by size:
2438 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5810
AflIII A'CryG_T
Cuts at: 1203 3304 4906 5883 1203
Size: 2101 1602 977 1327
Fragments arranged by size:
2101 1602 1327 977
AgeI A'CCGG_T
Cuts at: 4838 4838
Size: 6007
AhdI GACnn_n'nnGTC
Cuts at: 4736 4736
Size: 6007
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6007
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6007
AlwNI CAG_nnn'CTG
Cuts at: 2876 2887 3750 5322 2876
Size: 11 863 1572 3561
Fragments arranged by size:
3561 1572 863 11
ApaLI G'TGCA_C
Cuts at: 4064 5220 4064
Size: 1156 4851
ApoI r'AATT_y
Cuts at: 1493 3316 3542 3694 5803 1493
Size: 1823 226 152 2109 1697
Fragments arranged by size:
2109 1823 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4561 160
Size: 872 3529 1606
Fragments arranged by size:
3529 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6007
AvaI C'yCGr_G
Cuts at: 1870 1943 2505 3332 1870
Size: 73 562 827 4545
Fragments arranged by size:
4545 827 562 73
BamHI G'GATC_C
Cuts at: 3337 3704 3337
Size: 367 5640
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2033 3136 3328 4783 618
Size: 325 217 627 246 1103 192 1455 1842
Fragments arranged by size:
1842 1455 1103 627 325 246 217 192
BanII G_rGCy'C
Cuts at: 1391 2534 2861 3016 3326 1391
Size: 1143 327 155 310 4072
Fragments arranged by size:
4072 1143 327 310 155
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2330 928
Size: 971 431 4605
Fragments arranged by size:
4605 971 431
BbvCI CC'TCA_GC
Cuts at: 2348 2348
Size: 6007
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2625 2659 4198 4232 1903
Size: 34 50 34 604 34 1539 34 3678
Fragments arranged by size:
3678 1539 604 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3899 5109 3899
Size: 1210 4797
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6007
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2087 3194 4618
Size: 107 122 71 631 605 415 1107 1424
Cuts at: 4618 136
Size: 1525
Fragments arranged by size:
1525 1424 1107 631 605 415 122 107
71
BglII A'GATC_T
Cuts at: 6002 6002
Size: 6007
BlpI GC'TnA_GC
Cuts at: 3424 3424
Size: 6007
Bme1580I G_kGCm'C
Cuts at: 1790 2906 4068 5224 1790
Size: 1116 1162 1156 2573
Fragments arranged by size:
2573 1162 1156 1116
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6007
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3738 4696 442
Size: 416 53 83 399 24 2321 958 1753
Fragments arranged by size:
2321 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6007
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2079 3000 3030 4667 1239
Size: 840 921 30 1637 2579
Fragments arranged by size:
2579 1637 921 840 30
Bpu10I CC'TnA_GC
Cuts at: 2348 2744 2855 2348
Size: 396 111 5500
Fragments arranged by size:
5500 396 111
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2161 2420 4132 4997 5295 2161
Size: 259 1712 865 298 2873
Fragments arranged by size:
2873 1712 865 298 259
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4670 882
Size: 617 3171 2219
Fragments arranged by size:
3171 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3798 493
Size: 711 2594 2702
Fragments arranged by size:
2702 2594 711
BsaBI GATnn'nnATC
Cuts at: 6001 6001
Size: 6007
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2560 3814 4196 4831
Size: 53 83 186 936 1027 1254 382 635
Cuts at: 4831 275
Size: 1451
Fragments arranged by size:
1451 1254 1027 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4440 4838 5112 5259 5670 4440
Size: 398 274 147 411 4777
Fragments arranged by size:
4777 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2032 2062 3232 3262 1178
Size: 30 165 30 629 30 1170 30 3923
Fragments arranged by size:
3923 1170 629 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2090 2423 2469 2529 2741 2744 2774 1186
Size: 904 333 46 60 212 3 30 4419
Fragments arranged by size:
4419 904 333 212 60 46 30 3
BseYI C'CCAG_C
Cuts at: 2985 5210 5706 2985
Size: 2225 496 3286
Fragments arranged by size:
3286 2225 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2734 2850 3024 1163
Size: 573 998 116 174 4146
Fragments arranged by size:
4146 998 573 174 116
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3457 4218 5246 1540
Size: 275 186 1456 761 1028 2301
Fragments arranged by size:
2301 1456 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2861 3016 4068 4153 5224 2861
Size: 155 1052 85 1071 3644
Fragments arranged by size:
3644 1071 1052 155 85
BsmI GAATG_Cn'
Cuts at: 2740 3518 3611 2740
Size: 778 93 5136
Fragments arranged by size:
5136 778 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2534 2861 2906 3016 3326 4068 4153
Size: 399 744 327 45 110 310 742 85
Cuts at: 4153 5224 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 744 742 399 327 310 110
85 45
BspEI T'CCGG_A
Cuts at: 5670 5670
Size: 6007
BspHI T'CATG_A
Cuts at: 2563 3896 5626 2563
Size: 1333 1730 2944
Fragments arranged by size:
2944 1730 1333
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2487 2764 2793 3362 844
Size: 1643 277 29 569 3489
Fragments arranged by size:
3489 1643 569 277 29
BsrBI CCG'CTC
Cuts at: 1394 1823 3894 1394
Size: 429 2071 3507
Fragments arranged by size:
3507 2071 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2928 4502 4676 65
Size: 1183 1680 1574 174 1396
Fragments arranged by size:
1680 1574 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2383 2631 4651 4838 1700
Size: 110 573 248 2020 187 2869
Fragments arranged by size:
2869 2020 573 248 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6007
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6007
BssSI C'ACGA_G
Cuts at: 4067 5079 4067
Size: 1012 4995
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2169 3047 794
Size: 1375 878 3754
Fragments arranged by size:
3754 1375 878
BstBI TT'CG_AA
Cuts at: 4811 4811
Size: 6007
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6007
BstXI CCAn_nnnn'nTGG
Cuts at: 3253 3253
Size: 6007
BstYI r'GATC_y
Cuts at: 1972 3337 3704 4089 4106 5558 5644 5656 6002
Size: 1365 367 385 17 1452 86 12 346
Cuts at: 6002 1972
Size: 1977
Fragments arranged by size:
1977 1452 1365 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2290 2342 2923 3153 3252 513
Size: 552 1225 52 581 230 99 3268
Fragments arranged by size:
3268 1225 581 552 230 99 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5268
Fragments arranged by size:
5268 683 56
BtsI GCAGTG_nn'
Cuts at: 2107 2862 3523 4317 4337 2107
Size: 755 661 794 20 3777
Fragments arranged by size:
3777 794 755 661 20
ClaI AT'CG_AT
Cuts at: 1554 3203 1554
Size: 1649 4358
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5972
DraI TTT'AAA
Cuts at: 3311 3311
Size: 6007
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5014 809
Size: 507 3698 1802
Fragments arranged by size:
3698 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2790 3294 3454
Size: 54 1425 50 275 18 960 504 160
Cuts at: 3454 4343 5972 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 960 889 504 275 160 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3454 1537
Size: 275 1642 4090
Fragments arranged by size:
4090 1642 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3937 1353
Size: 2584 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4594 4968 5114 387
Size: 1064 138 3005 374 146 1280
Fragments arranged by size:
3005 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2859 3014 2859
Size: 155 5852
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2079 3000 3030 4070 4667 5454 5722
Size: 393 447 921 30 1040 597 787 268
Cuts at: 5722 1239
Size: 1524
Fragments arranged by size:
1524 1040 921 787 597 447 393 268
30
EcoO109I rG'GnC_Cy
Cuts at: 2054 2413 2054
Size: 359 5648
EcoRI G'AATT_C
Cuts at: 3316 3694 3316
Size: 378 5629
EcoRV GAT'ATC
Cuts at: 2177 2578 2177
Size: 401 5606
FspI TGC'GCA
Cuts at: 3287 4513 3287
Size: 1226 4781
FspAI rTGC'GCAy
Cuts at: 3287 3287
Size: 6007
HaeII r_GCGC'y
Cuts at: 1678 5154 1678
Size: 3476 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4648 4680 4722 4754 1373
Size: 32 135 32 3076 32 42 32 2626
Fragments arranged by size:
3076 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3351 3597 669
Size: 988 281 1413 246 3079
Fragments arranged by size:
3079 1413 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6007
HpaI GTT'AAC
Cuts at: 3597 3597
Size: 6007
KpnI G_GTAC'C
Cuts at: 2037 3332 2037
Size: 1295 4712
MfeI C'AATT_G
Cuts at: 3120 3606 3120
Size: 486 5521
MluI A'CGCG_T
Cuts at: 5883 5883
Size: 6007
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2229 5121 5305 1305
Size: 924 2892 184 2007
Fragments arranged by size:
2892 2007 924 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2792 10
Size: 54 1425 343 960 3225
Fragments arranged by size:
3225 1425 960 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3251 3296 3965 4324 4483 518
Size: 648 2085 45 669 359 159 2042
Fragments arranged by size:
2085 2042 669 648 359 159 45
MspA1I CmG'CkG
Cuts at: 1606 2046 2366 3020 3104 3423 4100 5248 5493
Size: 440 320 654 84 319 677 1148 245
Cuts at: 5493 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 654 440 320 319 245
84
NaeI GCC'GGC
Cuts at: 2633 2633
Size: 6007
NcoI C'CATG_G
Cuts at: 513 1065 2290 2923 3153 3252 513
Size: 552 1225 633 230 99 3268
Fragments arranged by size:
3268 1225 633 552 230 99
NdeI CA'TA_TG
Cuts at: 387 2677 387
Size: 2290 3717
NgoMIV G'CCGG_C
Cuts at: 2631 2631
Size: 6007
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6007
NotI GC'GGCC_GC
Cuts at: 3454 3454
Size: 6007
NspI r_CATG'y
Cuts at: 1132 1229 3286 3365 4910 1132
Size: 97 2057 79 1545 2229
Fragments arranged by size:
2229 2057 1545 97 79
PasI CC'CwG_GG
Cuts at: 1348 3127 1348
Size: 1779 4228
PciI A'CATG_T
Cuts at: 4906 4906
Size: 6007
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3133 3217 1108
Size: 2025 84 3898
Fragments arranged by size:
3898 2025 84
PmeI GTTT'AAAC
Cuts at: 3311 3311
Size: 6007
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4076 4108 5589 5621 4076
Size: 32 1481 32 4462
Fragments arranged by size:
4462 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2413 2413
Size: 6007
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6007
PsiI TTA'TAA
Cuts at: 3577 3577
Size: 6007
PstI C_TGCA'G
Cuts at: 830 3359 830
Size: 2529 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6007
PvuII CAG'CTG
Cuts at: 2366 3020 3104 2366
Size: 654 84 5269
Fragments arranged by size:
5269 654 84
SacI G_AGCT'C
Cuts at: 2861 3016 2861
Size: 155 5852
SalI G'TCGA_C
Cuts at: 1936 3349 1936
Size: 1413 4594
SbfI CC_TGCA'GG
Cuts at: 3359 3359
Size: 6007
ScaI AGT'ACT
Cuts at: 4255 5967 4255
Size: 1712 4295
SfcI C'TryA_G
Cuts at: 826 1045 2128 2966 3355 4490 5171 5362 826
Size: 219 1083 838 389 1135 681 191 1471
Fragments arranged by size:
1471 1135 1083 838 681 389 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6007
SgrAI Cr'CCGG_yG
Cuts at: 1700 2631 1700
Size: 931 5076
SmaI CCC'GGG
Cuts at: 1872 3334 1872
Size: 1462 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2140 2435 4111 5012 5274 820
Size: 197 926 197 295 1676 901 262 1553
Fragments arranged by size:
1676 1553 926 901 295 262 197 197
SnaBI TAC'GTA
Cuts at: 493 3798 493
Size: 3305 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6007
SphI G_CATG'C
Cuts at: 1132 3286 3365 1132
Size: 2154 79 3774
Fragments arranged by size:
3774 2154 79
SspI AAT'ATT
Cuts at: 5 52 3931 5
Size: 47 3879 2081
Fragments arranged by size:
3879 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6007
StyI C'CwwG_G
Cuts at: 513 1065 2290 2298 2371 2841 2923 3153 3216
Size: 552 1225 8 73 470 82 230 63
Cuts at: 3216 3252 3446 513
Size: 36 194 3074
Fragments arranged by size:
3074 1225 552 470 230 194 82 73
63 36 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2199 4034 4051 4204 4389 4834 325
Size: 1255 619 1835 17 153 185 445 1498
Fragments arranged by size:
1835 1498 1255 619 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4253 5965 96
Size: 274 80 33 51 1443 2276 1712 138
Fragments arranged by size:
2276 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2032 2064 3232 3264 5967 5999 2032
Size: 32 1168 32 2703 32 2040
Fragments arranged by size:
2703 2040 1168 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2559 1316
Size: 1243 4764
XbaI T'CTAG_A
Cuts at: 3343 3343
Size: 6007
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3160 3250 3160
Size: 90 5917
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6007
XmaI C'CCGG_G
Cuts at: 1870 3332 1870
Size: 1462 4545
XmnI GAAnn'nnTTC
Cuts at: 4136 4136
Size: 6007
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2561 3815 276
Size: 53 83 186 936 1027 1254 2468
Fragments arranged by size:
2468 1254 1027 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI
FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI FalI
FseI KasI NarI NruI NsiI PacI PfoI PmlI
PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI