Restriction map for
pFN21AB8826
(Circular) (Six-base) MAPSORT of: pFN21AB8826.seq Check: 9664 from: 1 to: 5950
pFN21AB8826 5950 bp
With 182 enzymes: SgfI *
March 28, 2012 12:03 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3760 278
Size: 53 83 186 936 2224 2468
Fragments arranged by size:
2468 2224 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2297 3293 1937
Size: 360 996 4594
Fragments arranged by size:
4594 996 360
Acc65I G'GTAC_C
Cuts at: 3271 3271
Size: 5950
AclI AA'CG_TT
Cuts at: 1563 4077 4450 1563
Size: 2514 373 3063
Fragments arranged by size:
3063 2514 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2955 4013 5397 5665 1632
Size: 1323 1058 1384 268 1917
Fragments arranged by size:
1917 1384 1323 1058 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5753
AflIII A'CryG_T
Cuts at: 1203 4849 5826 1203
Size: 3646 977 1327
Fragments arranged by size:
3646 1327 977
AgeI A'CCGG_T
Cuts at: 2490 4781 2490
Size: 2291 3659
AhdI GACnn_n'nnGTC
Cuts at: 4679 4679
Size: 5950
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5950
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2441 1184
Size: 1257 4693
AlwNI CAG_nnn'CTG
Cuts at: 3693 5265 3693
Size: 1572 4378
ApaLI G'TGCA_C
Cuts at: 4007 5163 4007
Size: 1156 4794
ApoI r'AATT_y
Cuts at: 1493 3259 3485 3637 5746 1493
Size: 1766 226 152 2109 1697
Fragments arranged by size:
2109 1766 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4504 160
Size: 872 3472 1606
Fragments arranged by size:
3472 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5950
AvaI C'yCGr_G
Cuts at: 1870 1943 2189 3275 1870
Size: 73 246 1086 4545
Fragments arranged by size:
4545 1086 246 73
BamHI G'GATC_C
Cuts at: 3280 3647 3280
Size: 367 5583
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2065 2898 3271 4726 618
Size: 325 217 627 278 833 373 1455 1842
Fragments arranged by size:
1842 1455 833 627 373 325 278 217
BanII G_rGCy'C
Cuts at: 1391 3269 1391
Size: 1878 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4979
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2347 2381 4141 4175 1903
Size: 34 410 34 1760 34 3678
Fragments arranged by size:
3678 1760 410 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3842 5052 3842
Size: 1210 4740
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5950
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4561 136
Size: 107 122 71 631 605 2889 1525
Fragments arranged by size:
2889 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2460 5945 2460
Size: 3485 2465
BlpI GC'TnA_GC
Cuts at: 2889 3367 2889
Size: 478 5472
Bme1580I G_kGCm'C
Cuts at: 1790 2070 4011 5167 1790
Size: 280 1941 1156 2573
Fragments arranged by size:
2573 1941 1156 280
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5950
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2629 3681 4639
Size: 416 53 83 399 24 1212 1052 958
Cuts at: 4639 442
Size: 1753
Fragments arranged by size:
1753 1212 1052 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5950
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2360 2433 4610 1239
Size: 1121 73 2177 2579
Fragments arranged by size:
2579 2177 1121 73
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4075 4940 5238 4075
Size: 865 298 4787
Fragments arranged by size:
4787 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2323 4613 882
Size: 617 824 2290 2219
Fragments arranged by size:
2290 2219 824 617
BsaAI yAC'GTr
Cuts at: 493 1204 3741 493
Size: 711 2537 2702
Fragments arranged by size:
2702 2537 711
BsaBI GATnn'nnATC
Cuts at: 5944 5944
Size: 5950
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3757 4139 4774 275
Size: 53 83 186 936 2224 382 635 1451
Fragments arranged by size:
2224 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2490 4383 4781 5055 5202 5613 2490
Size: 1893 398 274 147 411 2827
Fragments arranged by size:
2827 1893 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5725
Fragments arranged by size:
5725 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2153 2462 2641 2800 1186
Size: 967 309 179 159 4336
Fragments arranged by size:
4336 967 309 179 159
BseYI C'CCAG_C
Cuts at: 2401 5153 5649 2401
Size: 2752 496 2702
Fragments arranged by size:
2752 2702 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2071 1163
Size: 573 335 5042
Fragments arranged by size:
5042 573 335
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3400 4161 5189 1540
Size: 275 186 1399 761 1028 2301
Fragments arranged by size:
2301 1399 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2145 2601 4011 4096 5167 2145
Size: 456 1410 85 1071 2928
Fragments arranged by size:
2928 1410 1071 456 85
BsmI GAATG_Cn'
Cuts at: 3461 3554 3461
Size: 93 5857
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2070 2145 2601 3269 4011 4096 5167
Size: 399 280 75 456 668 742 85 1071
Cuts at: 5167 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 668 456 399 280 85
75
BspEI T'CCGG_A
Cuts at: 5613 5613
Size: 5950
BspHI T'CATG_A
Cuts at: 3839 5569 3839
Size: 1730 4220
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3305 844
Size: 2461 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3837 1394
Size: 429 2014 3507
Fragments arranged by size:
3507 2014 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3076 4445 4619 65
Size: 1183 1828 1369 174 1396
Fragments arranged by size:
1828 1396 1369 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2490 4594 4781 1700
Size: 110 680 2104 187 2869
Fragments arranged by size:
2869 2104 680 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5950
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5950
BssSI C'ACGA_G
Cuts at: 4010 5022 4010
Size: 1012 4938
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5950
BstBI TT'CG_AA
Cuts at: 4754 4754
Size: 5950
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5950
BstYI r'GATC_y
Cuts at: 1972 2460 2568 3280 3647 4032 4049 5501 5587
Size: 488 108 712 367 385 17 1452 86
Cuts at: 5587 5599 5945 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 712 488 385 367 346 108
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2322 3141 513
Size: 552 939 318 819 3322
Fragments arranged by size:
3322 939 819 552 318
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5211
Fragments arranged by size:
5211 683 56
BtsI GCAGTG_nn'
Cuts at: 2664 3466 4260 4280 2664
Size: 802 794 20 4334
Fragments arranged by size:
4334 802 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5950
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5915
DraI TTT'AAA
Cuts at: 3254 3254
Size: 5950
DraIII CAC_nnn'GTG
Cuts at: 2179 2179
Size: 5950
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4957 809
Size: 507 3641 1802
Fragments arranged by size:
3641 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2199 2773 3102
Size: 54 1425 50 275 18 369 574 329
Cuts at: 3102 3144 3397 4286 5915 8
Size: 42 253 889 1629 43
Fragments arranged by size:
1629 1425 889 574 369 329 275 253
54 50 43 42 18
EagI C'GGCC_G
Cuts at: 1537 1812 3397 1537
Size: 275 1585 4090
Fragments arranged by size:
4090 1585 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3880 1353
Size: 2527 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4537 4911 5057 387
Size: 1064 138 2948 374 146 1280
Fragments arranged by size:
2948 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2360 2433 2955 4013 4610 5397 5665
Size: 393 728 73 522 1058 597 787 268
Cuts at: 5665 1239
Size: 1524
Fragments arranged by size:
1524 1058 787 728 597 522 393 268
73
EcoRI G'AATT_C
Cuts at: 3259 3637 3259
Size: 378 5572
EcoRV GAT'ATC
Cuts at: 2958 2958
Size: 5950
FspI TGC'GCA
Cuts at: 4456 4456
Size: 5950
HaeII r_GCGC'y
Cuts at: 1678 5097 1678
Size: 3419 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4591 4623 4665 4697 1373
Size: 32 135 32 3019 32 42 32 2626
Fragments arranged by size:
3019 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3294 3540 669
Size: 988 281 1356 246 3079
Fragments arranged by size:
3079 1356 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3206 748
Size: 2458 3492
HpaI GTT'AAC
Cuts at: 3540 3540
Size: 5950
KpnI G_GTAC'C
Cuts at: 3275 3275
Size: 5950
MfeI C'AATT_G
Cuts at: 3549 3549
Size: 5950
MluI A'CGCG_T
Cuts at: 5826 5826
Size: 5950
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2129 2640 5064 5248 1305
Size: 824 511 2424 184 2007
Fragments arranged by size:
2424 2007 824 511 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2201 3104 3146 10
Size: 54 1425 343 369 903 42 2814
Fragments arranged by size:
2814 1425 903 369 343 54 42
MslI CAynn'nnrTG
Cuts at: 518 1166 2904 3908 4267 4426 518
Size: 648 1738 1004 359 159 2042
Fragments arranged by size:
2042 1738 1004 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2085 3366 4043 5191 5436 1606
Size: 479 1281 677 1148 245 2120
Fragments arranged by size:
2120 1281 1148 677 479 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2322 3141 513
Size: 552 939 318 819 3322
Fragments arranged by size:
3322 939 819 552 318
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5950
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5950
NotI GC'GGCC_GC
Cuts at: 3397 3397
Size: 5950
NspI r_CATG'y
Cuts at: 1132 1229 2331 3046 3308 4853 1132
Size: 97 1102 715 262 1545 2229
Fragments arranged by size:
2229 1545 1102 715 262 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5950
PciI A'CATG_T
Cuts at: 4849 4849
Size: 5950
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2689 1108
Size: 1581 4369
PmeI GTTT'AAAC
Cuts at: 3254 3254
Size: 5950
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4019 4051 5532 5564 4019
Size: 32 1481 32 4405
Fragments arranged by size:
4405 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5950
PsiI TTA'TAA
Cuts at: 3003 3520 3003
Size: 517 5433
PstI C_TGCA'G
Cuts at: 830 3302 830
Size: 2472 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5950
PvuII CAG'CTG
Cuts at: 2085 2085
Size: 5950
SalI G'TCGA_C
Cuts at: 1936 3292 1936
Size: 1356 4594
SbfI CC_TGCA'GG
Cuts at: 3302 3302
Size: 5950
ScaI AGT'ACT
Cuts at: 4198 5910 4198
Size: 1712 4238
SfcI C'TryA_G
Cuts at: 826 1045 3298 4433 5114 5305 826
Size: 219 2253 1135 681 191 1471
Fragments arranged by size:
2253 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5950
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5950
SmaI CCC'GGG
Cuts at: 1872 2191 3277 1872
Size: 319 1086 4545
Fragments arranged by size:
4545 1086 319
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4054 4955 5217 820
Size: 197 926 2111 901 262 1553
Fragments arranged by size:
2111 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3741 493
Size: 3248 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5950
SphI G_CATG'C
Cuts at: 1132 3308 1132
Size: 2176 3774
SspI AAT'ATT
Cuts at: 5 52 3874 5
Size: 47 3822 2081
Fragments arranged by size:
3822 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5950
StyI C'CwwG_G
Cuts at: 513 1065 2004 2322 3141 3195 3389 513
Size: 552 939 318 819 54 194 3074
Fragments arranged by size:
3074 939 819 552 318 194 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3977 3994 4147 4332 4777 325
Size: 1255 2397 17 153 185 445 1498
Fragments arranged by size:
2397 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4196 5908 96
Size: 274 80 33 51 1443 2219 1712 138
Fragments arranged by size:
2219 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5910 5942 5910
Size: 32 5918
Tth111I GACn'n_nGTC
Cuts at: 1316 2293 1316
Size: 977 4973
XbaI T'CTAG_A
Cuts at: 3286 3286
Size: 5950
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2311 2384 2311
Size: 73 5877
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5950
XmaI C'CCGG_G
Cuts at: 1870 2189 3275 1870
Size: 319 1086 4545
Fragments arranged by size:
4545 1086 319
XmnI GAAnn'nnTTC
Cuts at: 4079 4079
Size: 5950
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3758 276
Size: 53 83 186 936 2224 2468
Fragments arranged by size:
2468 2224 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI