Restriction map for
pFN21AB8824
(Circular) (Six-base) MAPSORT of: pFN21AB8824.seq Check: 6091 from: 1 to: 5947
pFN21AB8824 5947 bp
With 182 enzymes: SgfI *
February 27, 2009 14:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3757 278
Size: 53 83 186 936 2221 2468
Fragments arranged by size:
2468 2221 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3290 1937
Size: 1353 4594
Acc65I G'GTAC_C
Cuts at: 3268 3268
Size: 5947
AclI AA'CG_TT
Cuts at: 1563 4074 4447 1563
Size: 2511 373 3063
Fragments arranged by size:
3063 2511 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2299 2966 4010 5394 5662 1632
Size: 667 667 1044 1384 268 1917
Fragments arranged by size:
1917 1384 1044 667 667 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5750
AflIII A'CryG_T
Cuts at: 1203 2287 4846 5823 1203
Size: 1084 2559 977 1327
Fragments arranged by size:
2559 1327 1084 977
AgeI A'CCGG_T
Cuts at: 4778 4778
Size: 5947
AhdI GACnn_n'nnGTC
Cuts at: 4676 4676
Size: 5947
AleI CACnn'nnGTG
Cuts at: 1166 2024 2237 1166
Size: 858 213 4876
Fragments arranged by size:
4876 858 213
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2318 1184
Size: 1134 4813
AlwNI CAG_nnn'CTG
Cuts at: 2848 2944 3122 3690 5262 2848
Size: 96 178 568 1572 3533
Fragments arranged by size:
3533 1572 568 178 96
ApaI G_GGCC'C
Cuts at: 2884 2884
Size: 5947
ApaLI G'TGCA_C
Cuts at: 4004 5160 4004
Size: 1156 4791
ApoI r'AATT_y
Cuts at: 1493 3256 3482 3634 5743 1493
Size: 1763 226 152 2109 1697
Fragments arranged by size:
2109 1763 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4501 160
Size: 872 3469 1606
Fragments arranged by size:
3469 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5947
AvaI C'yCGr_G
Cuts at: 1870 1943 3272 1870
Size: 73 1329 4545
Fragments arranged by size:
4545 1329 73
AvrII C'CTAG_G
Cuts at: 2218 2218
Size: 5947
BamHI G'GATC_C
Cuts at: 3277 3644 3277
Size: 367 5580
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2185 2384 2499 2812 2821
Size: 325 217 627 398 199 115 313 9
Cuts at: 2821 3268 4723 618
Size: 447 1455 1842
Fragments arranged by size:
1842 1455 627 447 398 325 313 217
199 115 9
BanII G_rGCy'C
Cuts at: 1391 2353 2478 2600 2884 3147 3266 1391
Size: 962 125 122 284 263 119 4072
Fragments arranged by size:
4072 962 284 263 125 122 119
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2503 2747 928
Size: 971 604 244 4128
Fragments arranged by size:
4128 971 604 244
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4138 4172 1903
Size: 34 50 34 2117 34 3678
Fragments arranged by size:
3678 2117 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3839 5049 3839
Size: 1210 4737
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5947
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2138 4558 136
Size: 107 122 71 631 605 466 2420 1525
Fragments arranged by size:
2420 1525 631 605 466 122 107 71
BglII A'GATC_T
Cuts at: 5942 5942
Size: 5947
BlpI GC'TnA_GC
Cuts at: 3364 3364
Size: 5947
Bme1580I G_kGCm'C
Cuts at: 1790 2502 2634 2817 2826 2884 4008 5164 1790
Size: 712 132 183 9 58 1124 1156 2573
Fragments arranged by size:
2573 1156 1124 712 183 132 58 9
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5947
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2858 3678 4636
Size: 416 53 83 399 24 1441 820 958
Cuts at: 4636 442
Size: 1753
Fragments arranged by size:
1753 1441 958 820 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5947
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3148 3148
Size: 5947
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2036 3064 4607 1239
Size: 797 1028 1543 2579
Fragments arranged by size:
2579 1543 1028 797
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2606 2638 4072 4937 5235 2606
Size: 32 1434 865 298 3318
Fragments arranged by size:
3318 1434 865 298 32
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4610 882
Size: 617 3111 2219
Fragments arranged by size:
3111 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2288 3738 493
Size: 711 1084 1450 2702
Fragments arranged by size:
2702 1450 1084 711
BsaBI GATnn'nnATC
Cuts at: 5941 5941
Size: 5947
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3754 4136 4771 275
Size: 53 83 186 936 2221 382 635 1451
Fragments arranged by size:
2221 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4380 4778 5052 5199 5610 4380
Size: 398 274 147 411 4717
Fragments arranged by size:
4717 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3037 3067 1178
Size: 30 165 30 1634 30 4058
Fragments arranged by size:
4058 1634 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5947
BseYI C'CCAG_C
Cuts at: 3012 3076 3103 3233 5150 5646 3012
Size: 64 27 130 1917 496 3313
Fragments arranged by size:
3313 1917 496 130 64 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5374
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3397 4158 5186 1540
Size: 275 186 1396 761 1028 2301
Fragments arranged by size:
2301 1396 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2031 3147 4008 4093 5164 2031
Size: 1116 861 85 1071 2814
Fragments arranged by size:
2814 1116 1071 861 85
BsmI GAATG_Cn'
Cuts at: 3458 3551 3458
Size: 93 5854
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2031 2353 2478 2502 2600 2634 2817
Size: 399 241 322 125 24 98 34 183
Cuts at: 2817 2826 2884 3147 3266 4008 4093 5164 1391
Size: 9 58 263 119 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 322 263 241 183
125 119 98 85 58 34 24 9
BspEI T'CCGG_A
Cuts at: 5610 5610
Size: 5947
BspHI T'CATG_A
Cuts at: 3836 5566 3836
Size: 1730 4217
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2598 3171 3302 844
Size: 1754 573 131 3489
Fragments arranged by size:
3489 1754 573 131
BsrBI CCG'CTC
Cuts at: 1394 1823 3834 1394
Size: 429 2011 3507
Fragments arranged by size:
3507 2011 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4442 4616 65
Size: 1183 3194 174 1396
Fragments arranged by size:
3194 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2802 4591 4778 1700
Size: 110 992 1789 187 2869
Fragments arranged by size:
2869 1789 992 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5947
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5947
BssSI C'ACGA_G
Cuts at: 4007 5019 4007
Size: 1012 4935
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3055 794
Size: 2261 3686
BstBI TT'CG_AA
Cuts at: 4751 4751
Size: 5947
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5947
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5947
BstYI r'GATC_y
Cuts at: 1972 3118 3277 3644 4029 4046 5498 5584 5596
Size: 1146 159 367 385 17 1452 86 12
Cuts at: 5596 5942 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1146 385 367 346 159 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2703 2703
Size: 5947
BtgI C'CryG_G
Cuts at: 513 1065 2854 2983 513
Size: 552 1789 129 3477
Fragments arranged by size:
3477 1789 552 129
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5208
Fragments arranged by size:
5208 683 56
BtsI GCAGTG_nn'
Cuts at: 2167 2573 2904 3463 4257 4277 2167
Size: 406 331 559 794 20 3837
Fragments arranged by size:
3837 794 559 406 331 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5947
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5912
DraI TTT'AAA
Cuts at: 3251 3251
Size: 5947
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4954 809
Size: 507 3638 1802
Fragments arranged by size:
3638 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2139 2448 2769
Size: 54 1425 50 275 18 309 309 321
Cuts at: 2769 2885 3394 4283 5912 8
Size: 116 509 889 1629 43
Fragments arranged by size:
1629 1425 889 509 321 309 309 275
116 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3394 1537
Size: 275 1582 4090
Fragments arranged by size:
4090 1582 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2175 3877 1353
Size: 822 1702 3423
Fragments arranged by size:
3423 1702 822
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4534 4908 5054 387
Size: 1064 138 2945 374 146 1280
Fragments arranged by size:
2945 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3145 3145
Size: 5947
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2036 2299 2966 3064 4010 4607 5394
Size: 393 404 263 667 98 946 597 787
Cuts at: 5394 5662 1239
Size: 268 1524
Fragments arranged by size:
1524 946 787 667 597 404 393 268
263 98
EcoO109I rG'GnC_Cy
Cuts at: 2094 2880 2094
Size: 786 5161
EcoRI G'AATT_C
Cuts at: 3256 3634 3256
Size: 378 5569
FspI TGC'GCA
Cuts at: 4453 4453
Size: 5947
HaeII r_GCGC'y
Cuts at: 1678 3100 5094 1678
Size: 1422 1994 2531
Fragments arranged by size:
2531 1994 1422
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2627 2659 2850 2882 4588
Size: 32 135 32 1055 32 191 32 1706
Cuts at: 4588 4620 4662 4694 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1706 1055 191 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2956 3291 3537 669
Size: 988 281 1018 335 246 3079
Fragments arranged by size:
3079 1018 988 335 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5947
HpaI GTT'AAC
Cuts at: 3537 3537
Size: 5947
KpnI G_GTAC'C
Cuts at: 3272 3272
Size: 5947
MfeI C'AATT_G
Cuts at: 3546 3546
Size: 5947
MluI A'CGCG_T
Cuts at: 5823 5823
Size: 5947
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5061 5245 1305
Size: 3756 184 2007
Fragments arranged by size:
3756 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2450 2771 10
Size: 54 1425 343 618 321 3186
Fragments arranged by size:
3186 1425 618 343 321 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2024 2237 2324 2505 2969 3905 4264
Size: 648 858 213 87 181 464 936 359
Cuts at: 4264 4423 518
Size: 159 2042
Fragments arranged by size:
2042 936 858 648 464 359 213 181
159 87
MspA1I CmG'CkG
Cuts at: 1606 2192 2547 3016 3363 4040 5188 5433 1606
Size: 586 355 469 347 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 586 469 355 347 245
NaeI GCC'GGC
Cuts at: 2804 2804
Size: 5947
NcoI C'CATG_G
Cuts at: 513 1065 2854 513
Size: 552 1789 3606
Fragments arranged by size:
3606 1789 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5947
NgoMIV G'CCGG_C
Cuts at: 2802 2802
Size: 5947
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5947
NotI GC'GGCC_GC
Cuts at: 3394 3394
Size: 5947
NspI r_CATG'y
Cuts at: 1132 1229 2305 2329 2897 3305 4850 1132
Size: 97 1076 24 568 408 1545 2229
Fragments arranged by size:
2229 1545 1076 568 408 97 24
PasI CC'CwG_GG
Cuts at: 1348 2808 1348
Size: 1460 4487
PciI A'CATG_T
Cuts at: 4846 4846
Size: 5947
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2523 1108
Size: 1415 4532
PfoI T'CCnGG_A
Cuts at: 2457 2754 2457
Size: 297 5650
PmeI GTTT'AAAC
Cuts at: 3251 3251
Size: 5947
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4016 4048 5529 5561 4016
Size: 32 1481 32 4402
Fragments arranged by size:
4402 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2094 2094
Size: 5947
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5947
PsiI TTA'TAA
Cuts at: 3517 3517
Size: 5947
PspOMI G'GGCC_C
Cuts at: 2880 2880
Size: 5947
PstI C_TGCA'G
Cuts at: 830 2595 3299 830
Size: 1765 704 3478
Fragments arranged by size:
3478 1765 704
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5947
PvuII CAG'CTG
Cuts at: 2192 3016 2192
Size: 824 5123
SacI G_AGCT'C
Cuts at: 3147 3147
Size: 5947
SalI G'TCGA_C
Cuts at: 1936 3289 1936
Size: 1353 4594
SbfI CC_TGCA'GG
Cuts at: 3299 3299
Size: 5947
ScaI AGT'ACT
Cuts at: 4195 5907 4195
Size: 1712 4235
SfcI C'TryA_G
Cuts at: 826 1045 2193 2591 3295 4430 5111 5302 826
Size: 219 1148 398 704 1135 681 191 1471
Fragments arranged by size:
1471 1148 1135 704 681 398 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5947
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5947
SmaI CCC'GGG
Cuts at: 1872 3274 1872
Size: 1402 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2621 2653 4051 4952 5214 820
Size: 197 926 678 32 1398 901 262 1553
Fragments arranged by size:
1553 1398 926 901 678 262 197 32
SnaBI TAC'GTA
Cuts at: 493 3738 493
Size: 3245 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5947
SphI G_CATG'C
Cuts at: 1132 2897 3305 1132
Size: 1765 408 3774
Fragments arranged by size:
3774 1765 408
SspI AAT'ATT
Cuts at: 5 52 3871 5
Size: 47 3819 2081
Fragments arranged by size:
3819 2081 47
StuI AGG'CCT
Cuts at: 1517 2057 2370 1517
Size: 540 313 5094
Fragments arranged by size:
5094 540 313
StyI C'CwwG_G
Cuts at: 513 1065 2218 2666 2682 2854 3386 513
Size: 552 1153 448 16 172 532 3074
Fragments arranged by size:
3074 1153 552 532 448 172 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2800 3026 3068 3974 3991 4144 4329
Size: 1255 1220 226 42 906 17 153 185
Cuts at: 4329 4774 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1220 906 445 226 185 153
42 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2373 4193 5905
Size: 274 80 33 51 1443 396 1820 1712
Cuts at: 5905 96
Size: 138
Fragments arranged by size:
1820 1712 1443 396 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2524 2556 3037 3069 5907 5939 2524
Size: 32 481 32 2838 32 2532
Fragments arranged by size:
2838 2532 481 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5947
XbaI T'CTAG_A
Cuts at: 3283 3283
Size: 5947
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2196 2196
Size: 5947
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5947
XmaI C'CCGG_G
Cuts at: 1870 3272 1870
Size: 1402 4545
XmnI GAAnn'nnTTC
Cuts at: 4076 4076
Size: 5947
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3755 276
Size: 53 83 186 936 2221 2468
Fragments arranged by size:
2468 2221 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PmlI
PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI