Restriction map for
pFN21AB8822
(Circular) (Six-base) MAPSORT of: pFN21AB8822.seq Check: 635 from: 1 to: 5893
pFN21AB8822 5893 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2262 2513 2262
Size: 251 5642
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3703 278
Size: 53 83 186 936 2167 2468
Fragments arranged by size:
2468 2167 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3236 1937
Size: 1299 4594
Acc65I G'GTAC_C
Cuts at: 2488 3214 2488
Size: 726 5167
AclI AA'CG_TT
Cuts at: 1563 4020 4393 1563
Size: 2457 373 3063
Fragments arranged by size:
3063 2457 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2207 3956 5340 5608 1632
Size: 575 1749 1384 268 1917
Fragments arranged by size:
1917 1749 1384 575 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5696
AflIII A'CryG_T
Cuts at: 1203 4792 5769 1203
Size: 3589 977 1327
Fragments arranged by size:
3589 1327 977
AgeI A'CCGG_T
Cuts at: 2267 4724 2267
Size: 2457 3436
AhdI GACnn_n'nnGTC
Cuts at: 4622 4622
Size: 5893
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5893
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5893
AlwNI CAG_nnn'CTG
Cuts at: 2638 2725 2803 3636 5208 2638
Size: 87 78 833 1572 3323
Fragments arranged by size:
3323 1572 833 87 78
ApaI G_GGCC'C
Cuts at: 3171 3171
Size: 5893
ApaLI G'TGCA_C
Cuts at: 3950 5106 3950
Size: 1156 4737
ApoI r'AATT_y
Cuts at: 1493 2942 3202 3428 3580 5689 1493
Size: 1449 260 226 152 2109 1697
Fragments arranged by size:
2109 1697 1449 260 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4447 160
Size: 872 3415 1606
Fragments arranged by size:
3415 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5893
AvaI C'yCGr_G
Cuts at: 1870 1943 3048 3218 1870
Size: 73 1105 170 4545
Fragments arranged by size:
4545 1105 170 73
BamHI G'GATC_C
Cuts at: 3223 3590 3223
Size: 367 5526
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2105 2270 2488 3214 4669
Size: 325 217 627 318 165 218 726 1455
Cuts at: 4669 618
Size: 1842
Fragments arranged by size:
1842 1455 726 627 325 318 218 217
165
BanII G_rGCy'C
Cuts at: 1391 2298 2577 2926 3171 3212 1391
Size: 907 279 349 245 41 4072
Fragments arranged by size:
4072 907 349 279 245 41
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4922
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4084 4118 1903
Size: 34 2147 34 3678
Fragments arranged by size:
3678 2147 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3785 4995 3785
Size: 1210 4683
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5893
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4504 136
Size: 107 122 71 631 605 2832 1525
Fragments arranged by size:
2832 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5888 5888
Size: 5893
BlpI GC'TnA_GC
Cuts at: 2576 2853 3310 2576
Size: 277 457 5159
Fragments arranged by size:
5159 457 277
Bme1580I G_kGCm'C
Cuts at: 1790 3171 3954 5110 1790
Size: 1381 783 1156 2573
Fragments arranged by size:
2573 1381 1156 783
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5893
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2360 2748 3624
Size: 416 53 83 399 24 943 388 876
Cuts at: 3624 4582 442
Size: 958 1753
Fragments arranged by size:
1753 958 943 876 416 399 388 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5893
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2862 4553 1239
Size: 1623 1691 2579
Fragments arranged by size:
2579 1691 1623
Bpu10I CC'TnA_GC
Cuts at: 2379 2993 2379
Size: 614 5279
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4018 4883 5181 4018
Size: 865 298 4730
Fragments arranged by size:
4730 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2823 4556 882
Size: 617 1324 1733 2219
Fragments arranged by size:
2219 1733 1324 617
BsaAI yAC'GTr
Cuts at: 493 1204 3684 493
Size: 711 2480 2702
Fragments arranged by size:
2702 2480 711
BsaBI GATnn'nnATC
Cuts at: 5887 5887
Size: 5893
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2022 3700 4082 4717
Size: 53 83 186 936 489 1678 382 635
Cuts at: 4717 275
Size: 1451
Fragments arranged by size:
1678 1451 936 635 489 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2267 2926 4326 4724 4998 5145 5556 2267
Size: 659 1400 398 274 147 411 2604
Fragments arranged by size:
2604 1400 659 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2346 2376 1178
Size: 30 165 30 943 30 4695
Fragments arranged by size:
4695 943 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2195 2288 2354 1186
Size: 1009 93 66 4725
Fragments arranged by size:
4725 1009 93 66
BseYI C'CCAG_C
Cuts at: 2053 2079 2898 5096 5592 2053
Size: 26 819 2198 496 2354
Fragments arranged by size:
2354 2198 819 496 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2197 2240 2485 1163
Size: 573 461 43 245 4571
Fragments arranged by size:
4571 573 461 245 43
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3343 4104 5132 1540
Size: 275 186 1342 761 1028 2301
Fragments arranged by size:
2301 1342 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2124 2435 2926 3111 3954 4039 5110 2124
Size: 311 491 185 843 85 1071 2907
Fragments arranged by size:
2907 1071 843 491 311 185 85
BsmI GAATG_Cn'
Cuts at: 3404 3497 3404
Size: 93 5800
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2124 2298 2435 2577 2926 3111 3171
Size: 399 334 174 137 142 349 185 60
Cuts at: 3171 3212 3954 4039 5110 1391
Size: 41 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 349 334 185 174
142 137 85 60 41
BspEI T'CCGG_A
Cuts at: 2926 5556 2926
Size: 2630 3263
BspHI T'CATG_A
Cuts at: 3782 5512 3782
Size: 1730 4163
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2181 2262 2513 2724 3248 844
Size: 1337 81 251 211 524 3489
Fragments arranged by size:
3489 1337 524 251 211 81
BsrBI CCG'CTC
Cuts at: 1394 1823 3780 1394
Size: 429 1957 3507
Fragments arranged by size:
3507 1957 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2122 2367 4388 4562 65
Size: 1183 874 245 2021 174 1396
Fragments arranged by size:
2021 1396 1183 874 245 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2267 2529 4537 4724 1700
Size: 110 457 262 2008 187 2869
Fragments arranged by size:
2869 2008 457 262 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5893
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5893
BssSI C'ACGA_G
Cuts at: 3953 4965 3953
Size: 1012 4881
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5893
BstBI TT'CG_AA
Cuts at: 4697 4697
Size: 5893
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5893
BstXI CCAn_nnnn'nTGG
Cuts at: 2551 2683 3146 2551
Size: 132 463 5298
Fragments arranged by size:
5298 463 132
BstYI r'GATC_y
Cuts at: 1972 3223 3590 3975 3992 5444 5530 5542 5888
Size: 1251 367 385 17 1452 86 12 346
Cuts at: 5888 1972
Size: 1977
Fragments arranged by size:
1977 1452 1251 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2109 513
Size: 552 1044 4297
Fragments arranged by size:
4297 1044 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2329 487
Size: 683 56 1103 4051
Fragments arranged by size:
4051 1103 683 56
BtsI GCAGTG_nn'
Cuts at: 2587 3409 4203 4223 2587
Size: 822 794 20 4257
Fragments arranged by size:
4257 822 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5893
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5858
DraI TTT'AAA
Cuts at: 3159 3197 3159
Size: 38 5855
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4900 809
Size: 507 3584 1802
Fragments arranged by size:
3584 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2139 2451 2685
Size: 54 1425 50 275 18 309 312 234
Cuts at: 2685 3340 4229 5858 8
Size: 655 889 1629 43
Fragments arranged by size:
1629 1425 889 655 312 309 275 234
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3340 1537
Size: 275 1528 4090
Fragments arranged by size:
4090 1528 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2914 3823 1353
Size: 1561 909 3423
Fragments arranged by size:
3423 1561 909
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3125 4480 4854 5000 387
Size: 1064 138 1536 1355 374 146 1280
Fragments arranged by size:
1536 1355 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2924 2924
Size: 5893
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2207 2862 3956 4553 5340 5608 1239
Size: 393 575 655 1094 597 787 268 1524
Fragments arranged by size:
1524 1094 787 655 597 575 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2281 2281
Size: 5893
EcoO109I rG'GnC_Cy
Cuts at: 2750 3167 3168 2750
Size: 417 1 5475
Fragments arranged by size:
5475 417 1
EcoRI G'AATT_C
Cuts at: 3202 3580 3202
Size: 378 5515
FseI GG_CCGG'CC
Cuts at: 2533 2533
Size: 5893
FspI TGC'GCA
Cuts at: 4399 4399
Size: 5893
HaeII r_GCGC'y
Cuts at: 1678 5040 1678
Size: 3362 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2346 2378 2820 2852 2921
Size: 32 135 32 774 32 442 32 69
Cuts at: 2921 2953 4534 4566 4608 4640 1373
Size: 32 1581 32 42 32 2626
Fragments arranged by size:
2626 1581 774 442 135 69 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3237 3483 669
Size: 988 281 1299 246 3079
Fragments arranged by size:
3079 1299 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2381 748
Size: 1633 4260
HpaI GTT'AAC
Cuts at: 3483 3483
Size: 5893
KpnI G_GTAC'C
Cuts at: 2492 3218 2492
Size: 726 5167
MfeI C'AATT_G
Cuts at: 3492 3492
Size: 5893
MluI A'CGCG_T
Cuts at: 5769 5769
Size: 5893
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2700 5007 5191 1305
Size: 1395 2307 184 2007
Fragments arranged by size:
2307 2007 1395 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2141 2453 2687 10
Size: 54 1425 343 309 312 234 3216
Fragments arranged by size:
3216 1425 343 312 309 234 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2549 3851 4210 4369 518
Size: 648 1383 1302 359 159 2042
Fragments arranged by size:
2042 1383 1302 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3309 3986 5134 5379 1606
Size: 1703 677 1148 245 2120
Fragments arranged by size:
2120 1703 1148 677 245
NaeI GCC'GGC
Cuts at: 2531 2531
Size: 5893
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5341
NdeI CA'TA_TG
Cuts at: 387 3145 387
Size: 2758 3135
NgoMIV G'CCGG_C
Cuts at: 2529 2529
Size: 5893
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5893
NotI GC'GGCC_GC
Cuts at: 3340 3340
Size: 5893
NspI r_CATG'y
Cuts at: 1132 1229 3064 3251 4796 1132
Size: 97 1835 187 1545 2229
Fragments arranged by size:
2229 1835 1545 187 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5893
PciI A'CATG_T
Cuts at: 4792 4792
Size: 5893
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2725 2803 1108
Size: 1617 78 4198
Fragments arranged by size:
4198 1617 78
PfoI T'CCnGG_A
Cuts at: 2718 2718
Size: 5893
PmeI GTTT'AAAC
Cuts at: 3197 3197
Size: 5893
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3962 3994 5475 5507 3962
Size: 32 1481 32 4348
Fragments arranged by size:
4348 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2750 2750
Size: 5893
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5893
PsiI TTA'TAA
Cuts at: 3463 3463
Size: 5893
PspOMI G'GGCC_C
Cuts at: 3167 3167
Size: 5893
PstI C_TGCA'G
Cuts at: 830 2285 2510 3245 830
Size: 1455 225 735 3478
Fragments arranged by size:
3478 1455 735 225
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5893
SacI G_AGCT'C
Cuts at: 2926 2926
Size: 5893
SalI G'TCGA_C
Cuts at: 1936 3235 1936
Size: 1299 4594
SapI GCTCTTCn'nnn_
Cuts at: 2914 2914
Size: 5893
SbfI CC_TGCA'GG
Cuts at: 3245 3245
Size: 5893
ScaI AGT'ACT
Cuts at: 4141 5853 4141
Size: 1712 4181
SfcI C'TryA_G
Cuts at: 826 1045 2281 2493 2506 3241 4376 5057 5248
Size: 219 1236 212 13 735 1135 681 191
Cuts at: 5248 826
Size: 1471
Fragments arranged by size:
1471 1236 1135 735 681 219 212 191
13
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5893
SgrAI Cr'CCGG_yG
Cuts at: 1700 2267 1700
Size: 567 5326
SmaI CCC'GGG
Cuts at: 1872 3050 3220 1872
Size: 1178 170 4545
Fragments arranged by size:
4545 1178 170
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3997 4898 5160 820
Size: 197 926 2054 901 262 1553
Fragments arranged by size:
2054 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3684 493
Size: 3191 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5893
SphI G_CATG'C
Cuts at: 1132 3251 1132
Size: 2119 3774
SspI AAT'ATT
Cuts at: 5 52 3817 5
Size: 47 3765 2081
Fragments arranged by size:
3765 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5893
StyI C'CwwG_G
Cuts at: 513 1065 2136 2406 3332 513
Size: 552 1071 270 926 3074
Fragments arranged by size:
3074 1071 926 552 270
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2810 3920 3937 4090 4275 4720 325
Size: 1255 1230 1110 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1230 1110 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4139 5851 96
Size: 274 80 33 51 1443 2162 1712 138
Fragments arranged by size:
2162 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5853 5885 5853
Size: 32 5861
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5893
XbaI T'CTAG_A
Cuts at: 3229 3229
Size: 5893
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2191 2548 2800 2191
Size: 357 252 5284
Fragments arranged by size:
5284 357 252
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5893
XmaI C'CCGG_G
Cuts at: 1870 3048 3218 1870
Size: 1178 170 4545
Fragments arranged by size:
4545 1178 170
XmnI GAAnn'nnTTC
Cuts at: 4022 4022
Size: 5893
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3701 276
Size: 53 83 186 936 2167 2468
Fragments arranged by size:
2468 2167 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI
FspAI KasI NarI NruI NsiI PacI PmlI PspXI
PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI
SrfI SwaI