Restriction map for
pFN21AB8820
(Circular) (Six-base) MAPSORT of: pFN21AB8820.seq Check: 7086 from: 1 to: 5857
pFN21AB8820 5857 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3667 278
Size: 53 83 186 936 2131 2468
Fragments arranged by size:
2468 2131 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3200 1937
Size: 1263 4594
Acc65I G'GTAC_C
Cuts at: 3178 3178
Size: 5857
AclI AA'CG_TT
Cuts at: 1563 3984 4357 1563
Size: 2421 373 3063
Fragments arranged by size:
3063 2421 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2993 3920 5304 5572 1632
Size: 1361 927 1384 268 1917
Fragments arranged by size:
1917 1384 1361 927 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5660
AflIII A'CryG_T
Cuts at: 1203 4756 5733 1203
Size: 3553 977 1327
Fragments arranged by size:
3553 1327 977
AgeI A'CCGG_T
Cuts at: 4688 4688
Size: 5857
AhdI GACnn_n'nnGTC
Cuts at: 4586 4586
Size: 5857
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5857
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5857
AlwNI CAG_nnn'CTG
Cuts at: 2971 3600 5172 2971
Size: 629 1572 3656
Fragments arranged by size:
3656 1572 629
ApaLI G'TGCA_C
Cuts at: 3914 5070 3914
Size: 1156 4701
ApoI r'AATT_y
Cuts at: 1493 3166 3392 3544 5653 1493
Size: 1673 226 152 2109 1697
Fragments arranged by size:
2109 1697 1673 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4411 160
Size: 872 3379 1606
Fragments arranged by size:
3379 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5857
AvaI C'yCGr_G
Cuts at: 1870 1943 2475 3182 1870
Size: 73 532 707 4545
Fragments arranged by size:
4545 707 532 73
AvrII C'CTAG_G
Cuts at: 2653 2653
Size: 5857
BamHI G'GATC_C
Cuts at: 3187 3554 3187
Size: 367 5490
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3003 3010 3178 4633 618
Size: 325 217 627 1216 7 168 1455 1842
Fragments arranged by size:
1842 1455 1216 627 325 217 168 7
BanII G_rGCy'C
Cuts at: 1391 3176 1391
Size: 1785 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2137 2149 2329 928
Size: 971 238 12 180 4456
Fragments arranged by size:
4456 971 238 180 12
BbvCI CC'TCA_GC
Cuts at: 2186 2468 2723 2186
Size: 282 255 5320
Fragments arranged by size:
5320 282 255
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4048 4082 1903
Size: 34 2111 34 3678
Fragments arranged by size:
3678 2111 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3749 4959 3749
Size: 1210 4647
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5857
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2236 4468 136
Size: 107 122 71 631 605 564 2232 1525
Fragments arranged by size:
2232 1525 631 605 564 122 107 71
BglII A'GATC_T
Cuts at: 2250 5852 2250
Size: 3602 2255
BlpI GC'TnA_GC
Cuts at: 2451 3274 2451
Size: 823 5034
Bme1580I G_kGCm'C
Cuts at: 1790 3918 5074 1790
Size: 2128 1156 2573
Fragments arranged by size:
2573 2128 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5857
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2147 3588 4546
Size: 416 53 83 399 24 730 1441 958
Cuts at: 4546 442
Size: 1753
Fragments arranged by size:
1753 1441 958 730 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5857
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2456 2870 2932 4517 1239
Size: 1217 414 62 1585 2579
Fragments arranged by size:
2579 1585 1217 414 62
Bpu10I CC'TnA_GC
Cuts at: 2186 2468 2723 2186
Size: 282 255 5320
Fragments arranged by size:
5320 282 255
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3982 4847 5145 3982
Size: 865 298 4694
Fragments arranged by size:
4694 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4520 882
Size: 617 3021 2219
Fragments arranged by size:
3021 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2227 3648 493
Size: 711 1023 1421 2702
Fragments arranged by size:
2702 1421 1023 711
BsaBI GATnn'nnATC
Cuts at: 5851 5851
Size: 5857
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3664 4046 4681 275
Size: 53 83 186 936 2131 382 635 1451
Fragments arranged by size:
2131 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4290 4688 4962 5109 5520 4290
Size: 398 274 147 411 4627
Fragments arranged by size:
4627 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2005 2035 1178
Size: 30 165 30 602 30 5000
Fragments arranged by size:
5000 602 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5857
BseYI C'CCAG_C
Cuts at: 5060 5556 5060
Size: 496 5361
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5284
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3307 4068 5096 1540
Size: 275 186 1306 761 1028 2301
Fragments arranged by size:
2301 1306 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2369 3918 4003 5074 2369
Size: 1549 85 1071 3152
Fragments arranged by size:
3152 1549 1071 85
BsmI GAATG_Cn'
Cuts at: 3368 3461 3368
Size: 93 5764
BsmBI CGTCTCn'nnnn_
Cuts at: 2533 2533
Size: 5857
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2369 3176 3918 4003 5074 1391
Size: 399 579 807 742 85 1071 2174
Fragments arranged by size:
2174 1071 807 742 579 399 85
BspEI T'CCGG_A
Cuts at: 5520 5520
Size: 5857
BspHI T'CATG_A
Cuts at: 3746 5476 3746
Size: 1730 4127
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2844 3212 844
Size: 2000 368 3489
Fragments arranged by size:
3489 2000 368
BsrBI CCG'CTC
Cuts at: 1394 1823 3744 1394
Size: 429 1921 3507
Fragments arranged by size:
3507 1921 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4352 4526 65
Size: 1183 3104 174 1396
Fragments arranged by size:
3104 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4501 4688 1700
Size: 110 2691 187 2869
Fragments arranged by size:
2869 2691 187 110
BsrGI T'GTAC_A
Cuts at: 96 2551 96
Size: 2455 3402
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5857
BssSI C'ACGA_G
Cuts at: 3917 4929 3917
Size: 1012 4845
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5857
BstBI TT'CG_AA
Cuts at: 4661 4661
Size: 5857
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5857
BstYI r'GATC_y
Cuts at: 1972 2250 3187 3554 3939 3956 5408 5494 5506
Size: 278 937 367 385 17 1452 86 12
Cuts at: 5506 5852 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 937 385 367 346 278 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2319 513
Size: 552 939 315 4051
Fragments arranged by size:
4051 939 552 315
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2887 487
Size: 683 56 1661 3457
Fragments arranged by size:
3457 1661 683 56
BtsI GCAGTG_nn'
Cuts at: 2013 2130 2297 2914 3018 3373 4167 4187 2013
Size: 117 167 617 104 355 794 20 3683
Fragments arranged by size:
3683 794 617 355 167 117 104 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5857
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5822
DraI TTT'AAA
Cuts at: 2751 3161 2751
Size: 410 5447
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4864 809
Size: 507 3548 1802
Fragments arranged by size:
3548 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2268 2523
Size: 54 1425 50 275 18 177 261 255
Cuts at: 2523 3304 4193 5822 8
Size: 781 889 1629 43
Fragments arranged by size:
1629 1425 889 781 275 261 255 177
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3304 1537
Size: 275 1492 4090
Fragments arranged by size:
4090 1492 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2633 3787 1353
Size: 1280 1154 3423
Fragments arranged by size:
3423 1280 1154
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2254 2951 4444 4818 4964 387
Size: 1064 138 665 697 1493 374 146 1280
Fragments arranged by size:
1493 1280 1064 697 665 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2456 2870 2932 2993 3920 4517 5304
Size: 393 824 414 62 61 927 597 787
Cuts at: 5304 5572 1239
Size: 268 1524
Fragments arranged by size:
1524 927 824 787 597 414 393 268
62 61
EcoNI CCTnn'n_nnAGG
Cuts at: 2461 2461
Size: 5857
EcoO109I rG'GnC_Cy
Cuts at: 2472 2472
Size: 5857
EcoRI G'AATT_C
Cuts at: 3166 3544 3166
Size: 378 5479
FspI TGC'GCA
Cuts at: 4363 4363
Size: 5857
HaeII r_GCGC'y
Cuts at: 1678 5004 1678
Size: 3326 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3095 3127 4498 4530 4572
Size: 32 135 32 1523 32 1371 32 42
Cuts at: 4572 4604 1373
Size: 32 2626
Fragments arranged by size:
2626 1523 1371 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3201 3447 669
Size: 988 281 1263 246 3079
Fragments arranged by size:
3079 1263 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5857
HpaI GTT'AAC
Cuts at: 3447 3447
Size: 5857
KpnI G_GTAC'C
Cuts at: 3182 3182
Size: 5857
MfeI C'AATT_G
Cuts at: 3456 3456
Size: 5857
MluI A'CGCG_T
Cuts at: 5733 5733
Size: 5857
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2301 2811 4971 5155 1305
Size: 996 510 2160 184 2007
Fragments arranged by size:
2160 2007 996 510 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2270 10
Size: 54 1425 343 438 3597
Fragments arranged by size:
3597 1425 438 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2318 3815 4174 4333 518
Size: 648 1152 1497 359 159 2042
Fragments arranged by size:
2042 1497 1152 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2190 3042 3273 3950 5098 5343 1606
Size: 584 852 231 677 1148 245 2120
Fragments arranged by size:
2120 1148 852 677 584 245 231
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4366
Fragments arranged by size:
4366 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5857
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5857
NotI GC'GGCC_GC
Cuts at: 3304 3304
Size: 5857
NspI r_CATG'y
Cuts at: 1132 1229 3215 4760 1132
Size: 97 1986 1545 2229
Fragments arranged by size:
2229 1986 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5857
PciI A'CATG_T
Cuts at: 4756 4756
Size: 5857
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2508 1108
Size: 1400 4457
PmeI GTTT'AAAC
Cuts at: 3161 3161
Size: 5857
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3926 3958 5439 5471 3926
Size: 32 1481 32 4312
Fragments arranged by size:
4312 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2472 2472
Size: 5857
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5857
PsiI TTA'TAA
Cuts at: 3427 3427
Size: 5857
PstI C_TGCA'G
Cuts at: 830 2114 2764 3209 830
Size: 1284 650 445 3478
Fragments arranged by size:
3478 1284 650 445
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5857
PvuII CAG'CTG
Cuts at: 3042 3042
Size: 5857
SalI G'TCGA_C
Cuts at: 1936 3199 1936
Size: 1263 4594
SapI GCTCTTCn'nnn_
Cuts at: 2633 2633
Size: 5857
SbfI CC_TGCA'GG
Cuts at: 3209 3209
Size: 5857
ScaI AGT'ACT
Cuts at: 4105 5817 4105
Size: 1712 4145
SfcI C'TryA_G
Cuts at: 826 1045 2110 2161 2760 3205 4340 5021 5212
Size: 219 1065 51 599 445 1135 681 191
Cuts at: 5212 826
Size: 1471
Fragments arranged by size:
1471 1135 1065 681 599 445 219 191
51
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5857
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5857
SmaI CCC'GGG
Cuts at: 1872 2477 3184 1872
Size: 605 707 4545
Fragments arranged by size:
4545 707 605
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3961 4862 5124 820
Size: 197 926 2018 901 262 1553
Fragments arranged by size:
2018 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3648 493
Size: 3155 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5857
SphI G_CATG'C
Cuts at: 1132 3215 1132
Size: 2083 3774
SspI AAT'ATT
Cuts at: 5 52 3781 5
Size: 47 3729 2081
Fragments arranged by size:
3729 2081 47
StuI AGG'CCT
Cuts at: 1517 3029 1517
Size: 1512 4345
StyI C'CwwG_G
Cuts at: 513 1065 2004 2571 2653 2766 3097 3296 513
Size: 552 939 567 82 113 331 199 3074
Fragments arranged by size:
3074 939 567 552 331 199 113 82
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3884 3901 4054 4239 4684 325
Size: 1255 2304 17 153 185 445 1498
Fragments arranged by size:
2304 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2551 4103 5815
Size: 274 80 33 51 1443 574 1552 1712
Cuts at: 5815 96
Size: 138
Fragments arranged by size:
1712 1552 1443 574 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2005 2037 5817 5849 2005
Size: 32 3780 32 2013
Fragments arranged by size:
3780 2013 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5857
XbaI T'CTAG_A
Cuts at: 2872 3193 2872
Size: 321 5536
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2539 2539
Size: 5857
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5857
XmaI C'CCGG_G
Cuts at: 1870 2475 3182 1870
Size: 605 707 4545
Fragments arranged by size:
4545 707 605
XmnI GAAnn'nnTTC
Cuts at: 2627 2978 3986 2627
Size: 351 1008 4498
Fragments arranged by size:
4498 1008 351
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3665 276
Size: 53 83 186 936 2131 2468
Fragments arranged by size:
2468 2131 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BfrBI
BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI