Restriction map for
pFN21AB8817
(Circular) (Six-base) MAPSORT of: pFN21AB8817.seq Check: 5473 from: 1 to: 5848
pFN21AB8817 5848 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3658 278
Size: 53 83 186 936 2122 2468
Fragments arranged by size:
2468 2122 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2314 2463 3191 1937
Size: 377 149 728 4594
Fragments arranged by size:
4594 728 377 149
Acc65I G'GTAC_C
Cuts at: 3169 3169
Size: 5848
AclI AA'CG_TT
Cuts at: 1563 3975 4348 1563
Size: 2412 373 3063
Fragments arranged by size:
3063 2412 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2924 3911 5295 5563 1632
Size: 1292 987 1384 268 1917
Fragments arranged by size:
1917 1384 1292 987 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5651
AflIII A'CryG_T
Cuts at: 1203 4747 5724 1203
Size: 3544 977 1327
Fragments arranged by size:
3544 1327 977
AgeI A'CCGG_T
Cuts at: 4679 4679
Size: 5848
AhdI GACnn_n'nnGTC
Cuts at: 4577 4577
Size: 5848
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5848
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5848
AlwNI CAG_nnn'CTG
Cuts at: 2027 2398 2896 3591 5163 2027
Size: 371 498 695 1572 2712
Fragments arranged by size:
2712 1572 695 498 371
ApaLI G'TGCA_C
Cuts at: 3905 5061 3905
Size: 1156 4692
ApoI r'AATT_y
Cuts at: 1493 2234 3157 3383 3535 5644 1493
Size: 741 923 226 152 2109 1697
Fragments arranged by size:
2109 1697 923 741 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4402 160
Size: 872 3370 1606
Fragments arranged by size:
3370 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5848
AvaI C'yCGr_G
Cuts at: 1870 1943 3173 1870
Size: 73 1230 4545
Fragments arranged by size:
4545 1230 73
BamHI G'GATC_C
Cuts at: 3178 3545 3178
Size: 367 5481
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2380 3169 4624 618
Size: 325 217 627 593 789 1455 1842
Fragments arranged by size:
1842 1455 789 627 593 325 217
BanII G_rGCy'C
Cuts at: 1391 3167 1391
Size: 1776 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4877
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4039 4073 1903
Size: 34 2102 34 3678
Fragments arranged by size:
3678 2102 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2422 3740 4950 2422
Size: 1318 1210 3320
Fragments arranged by size:
3320 1318 1210
BclI T'GATC_A
Cuts at: 1548 2229 1548
Size: 681 5167
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2279 2540 4459
Size: 107 122 71 631 605 607 261 1919
Cuts at: 4459 136
Size: 1525
Fragments arranged by size:
1919 1525 631 607 605 261 122 107
71
BglII A'GATC_T
Cuts at: 2290 2481 5843 2290
Size: 191 3362 2295
Fragments arranged by size:
3362 2295 191
BlpI GC'TnA_GC
Cuts at: 3265 3265
Size: 5848
Bme1580I G_kGCm'C
Cuts at: 1790 2385 2536 3909 5065 1790
Size: 595 151 1373 1156 2573
Fragments arranged by size:
2573 1373 1156 595 151
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5848
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3579 4537 442
Size: 416 53 83 399 24 2162 958 1753
Fragments arranged by size:
2162 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5848
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2781 3113 4508 1239
Size: 1542 332 1395 2579
Fragments arranged by size:
2579 1542 1395 332
Bpu10I CC'TnA_GC
Cuts at: 2112 2112
Size: 5848
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3973 4838 5136 3973
Size: 865 298 4685
Fragments arranged by size:
4685 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4511 882
Size: 617 3012 2219
Fragments arranged by size:
3012 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3639 493
Size: 711 2435 2702
Fragments arranged by size:
2702 2435 711
BsaBI GATnn'nnATC
Cuts at: 2582 5842 2582
Size: 3260 2588
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3655 4037 4672 275
Size: 53 83 186 936 2122 382 635 1451
Fragments arranged by size:
2122 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4281 4679 4953 5100 5511 4281
Size: 398 274 147 411 4618
Fragments arranged by size:
4618 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5623
Fragments arranged by size:
5623 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2648 1186
Size: 1462 4386
BseYI C'CCAG_C
Cuts at: 5051 5547 5051
Size: 496 5352
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2040 1163
Size: 573 304 4971
Fragments arranged by size:
4971 573 304
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3298 4059 5087 1540
Size: 275 186 1297 761 1028 2301
Fragments arranged by size:
2301 1297 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2954 3909 3994 5065 2954
Size: 955 85 1071 3737
Fragments arranged by size:
3737 1071 955 85
BsmI GAATG_Cn'
Cuts at: 3359 3452 3359
Size: 93 5755
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2385 2536 2954 3167 3909 3994 5065
Size: 399 595 151 418 213 742 85 1071
Cuts at: 5065 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 595 418 399 213 151
85
BspEI T'CCGG_A
Cuts at: 5511 5511
Size: 5848
BspHI T'CATG_A
Cuts at: 3737 5467 3737
Size: 1730 4118
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3203 844
Size: 2359 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3735 1394
Size: 429 1912 3507
Fragments arranged by size:
3507 1912 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2268 4343 4517 65
Size: 1183 1020 2075 174 1396
Fragments arranged by size:
2075 1396 1183 1020 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2445 4492 4679 1700
Size: 110 635 2047 187 2869
Fragments arranged by size:
2869 2047 635 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5848
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5848
BssSI C'ACGA_G
Cuts at: 3908 4920 3908
Size: 1012 4836
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5848
BstBI TT'CG_AA
Cuts at: 4652 4652
Size: 5848
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5848
BstXI CCAn_nnnn'nTGG
Cuts at: 2812 2812
Size: 5848
BstYI r'GATC_y
Cuts at: 1972 2290 2481 3178 3545 3930 3947 5399 5485
Size: 318 191 697 367 385 17 1452 86
Cuts at: 5485 5497 5843 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 697 385 367 346 318 191
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4357
Fragments arranged by size:
4357 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5109
Fragments arranged by size:
5109 683 56
BtsI GCAGTG_nn'
Cuts at: 3364 4158 4178 3364
Size: 794 20 5034
Fragments arranged by size:
5034 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5848
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5813
DraI TTT'AAA
Cuts at: 3152 3152
Size: 5848
DraIII CAC_nnn'GTG
Cuts at: 2310 2380 2310
Size: 70 5778
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2891 4855 809
Size: 507 1575 1964 1802
Fragments arranged by size:
1964 1802 1575 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2553 3295 4184
Size: 54 1425 50 275 18 723 742 889
Cuts at: 4184 5813 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 742 723 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3295 1537
Size: 275 1483 4090
Fragments arranged by size:
4090 1483 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2633 3778 1353
Size: 1280 1145 3423
Fragments arranged by size:
3423 1280 1145
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4435 4809 4955 387
Size: 1064 138 2846 374 146 1280
Fragments arranged by size:
2846 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2781 2924 3113 3911 4508 5295 5563
Size: 393 1149 143 189 798 597 787 268
Cuts at: 5563 1239
Size: 1524
Fragments arranged by size:
1524 1149 798 787 597 393 268 189
143
EcoO109I rG'GnC_Cy
Cuts at: 2360 2395 2893 2360
Size: 35 498 5315
Fragments arranged by size:
5315 498 35
EcoRI G'AATT_C
Cuts at: 3157 3535 3157
Size: 378 5470
FspI TGC'GCA
Cuts at: 2249 4354 2249
Size: 2105 3743
FspAI rTGC'GCAy
Cuts at: 2249 2249
Size: 5848
HaeII r_GCGC'y
Cuts at: 1678 4995 1678
Size: 3317 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4489 4521 4563 4595 1373
Size: 32 135 32 2917 32 42 32 2626
Fragments arranged by size:
2917 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2878 3192 3438 669
Size: 988 281 940 314 246 3079
Fragments arranged by size:
3079 988 940 314 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5848
HpaI GTT'AAC
Cuts at: 3438 3438
Size: 5848
KpnI G_GTAC'C
Cuts at: 3173 3173
Size: 5848
MfeI C'AATT_G
Cuts at: 3447 3447
Size: 5848
MluI A'CGCG_T
Cuts at: 5724 5724
Size: 5848
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3021 4962 5146 1305
Size: 1716 1941 184 2007
Fragments arranged by size:
2007 1941 1716 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2555 10
Size: 54 1425 343 723 3303
Fragments arranged by size:
3303 1425 723 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2807 3806 4165 4324 518
Size: 648 1641 999 359 159 2042
Fragments arranged by size:
2042 1641 999 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2844 3027 3037 3264 3941 5089 5334 1606
Size: 1238 183 10 227 677 1148 245 2120
Fragments arranged by size:
2120 1238 1148 677 245 227 183 10
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4357
Fragments arranged by size:
4357 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5848
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5848
NotI GC'GGCC_GC
Cuts at: 3295 3295
Size: 5848
NspI r_CATG'y
Cuts at: 1132 1229 2248 3206 4751 1132
Size: 97 1019 958 1545 2229
Fragments arranged by size:
2229 1545 1019 958 97
PasI CC'CwG_GG
Cuts at: 1348 2392 1348
Size: 1044 4804
PciI A'CATG_T
Cuts at: 4747 4747
Size: 5848
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2044 2398 2896 1108
Size: 936 354 498 4060
Fragments arranged by size:
4060 936 498 354
PfoI T'CCnGG_A
Cuts at: 2562 2589 2758 2895 2562
Size: 27 169 137 5515
Fragments arranged by size:
5515 169 137 27
PmeI GTTT'AAAC
Cuts at: 3152 3152
Size: 5848
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3917 3949 5430 5462 3917
Size: 32 1481 32 4303
Fragments arranged by size:
4303 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2360 2893 2360
Size: 533 5315
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5848
PsiI TTA'TAA
Cuts at: 3418 3418
Size: 5848
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2142 2174 2981 3013 2142
Size: 32 807 32 4977
Fragments arranged by size:
4977 807 32 32
PstI C_TGCA'G
Cuts at: 830 3200 830
Size: 2370 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5848
PvuII CAG'CTG
Cuts at: 2844 2844
Size: 5848
SalI G'TCGA_C
Cuts at: 1936 3190 1936
Size: 1254 4594
SapI GCTCTTCn'nnn_
Cuts at: 2633 2633
Size: 5848
SbfI CC_TGCA'GG
Cuts at: 3200 3200
Size: 5848
ScaI AGT'ACT
Cuts at: 4096 5808 4096
Size: 1712 4136
SexAI A'CCwGG_T
Cuts at: 2376 2376
Size: 5848
SfcI C'TryA_G
Cuts at: 826 1045 2659 2998 3196 4331 5012 5203 826
Size: 219 1614 339 198 1135 681 191 1471
Fragments arranged by size:
1614 1471 1135 681 339 219 198 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5848
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5848
SmaI CCC'GGG
Cuts at: 1872 3175 1872
Size: 1303 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3952 4853 5115 820
Size: 197 926 2009 901 262 1553
Fragments arranged by size:
2009 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3639 493
Size: 3146 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5848
SphI G_CATG'C
Cuts at: 1132 2248 3206 1132
Size: 1116 958 3774
Fragments arranged by size:
3774 1116 958
SspI AAT'ATT
Cuts at: 5 52 3772 5
Size: 47 3720 2081
Fragments arranged by size:
3720 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5848
StyI C'CwwG_G
Cuts at: 513 1065 2004 2211 3287 513
Size: 552 939 207 1076 3074
Fragments arranged by size:
3074 1076 939 552 207
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3875 3892 4045 4230 4675 325
Size: 1255 2295 17 153 185 445 1498
Fragments arranged by size:
2295 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2404 2612 2910
Size: 274 80 33 51 1443 427 208 298
Cuts at: 2910 4094 5806 96
Size: 1184 1712 138
Fragments arranged by size:
1712 1443 1184 427 298 274 208 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5808 5840 5808
Size: 32 5816
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5848
XbaI T'CTAG_A
Cuts at: 3184 3184
Size: 5848
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2281 2756 2809 2281
Size: 475 53 5320
Fragments arranged by size:
5320 475 53
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5848
XmaI C'CCGG_G
Cuts at: 1870 3173 1870
Size: 1303 4545
XmnI GAAnn'nnTTC
Cuts at: 3977 3977
Size: 5848
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3656 276
Size: 53 83 186 936 2122 2468
Fragments arranged by size:
2468 2122 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PsrI PstI PvuI PvuII SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI
EcoNI EcoRV FalI FseI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI RsrII SacI
SacII SanDI SfiI SfoI SrfI SwaI