Restriction map for
pFN21AB8816
(Circular) (Six-base) MAPSORT of: pFN21AB8816.seq Check: 4181 from: 1 to: 5842
pFN21AB8816 5842 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3652 278
Size: 53 83 186 936 2116 2468
Fragments arranged by size:
2468 2116 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2610 3185 1937
Size: 673 575 4594
Fragments arranged by size:
4594 673 575
Acc65I G'GTAC_C
Cuts at: 2229 3163 2229
Size: 934 4908
AclI AA'CG_TT
Cuts at: 1563 3969 4342 1563
Size: 2406 373 3063
Fragments arranged by size:
3063 2406 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2277 2771 3905 5289 5557 1632
Size: 645 494 1134 1384 268 1917
Fragments arranged by size:
1917 1384 1134 645 494 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5645
AflIII A'CryG_T
Cuts at: 1203 4741 5718 1203
Size: 3538 977 1327
Fragments arranged by size:
3538 1327 977
AgeI A'CCGG_T
Cuts at: 4673 4673
Size: 5842
AhdI GACnn_n'nnGTC
Cuts at: 4571 4571
Size: 5842
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5842
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2742 1184
Size: 1558 4284
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3108 3140 3108
Size: 32 5810
AlwNI CAG_nnn'CTG
Cuts at: 2794 3585 5157 2794
Size: 791 1572 3479
Fragments arranged by size:
3479 1572 791
ApaI G_GGCC'C
Cuts at: 2854 2854
Size: 5842
ApaLI G'TGCA_C
Cuts at: 3899 5055 3899
Size: 1156 4686
ApoI r'AATT_y
Cuts at: 1493 2809 3020 3095 3151 3377 3529 5638 1493
Size: 1316 211 75 56 226 152 2109 1697
Fragments arranged by size:
2109 1697 1316 226 211 152 75 56
AseI AT'TA_AT
Cuts at: 160 1032 4396 160
Size: 872 3364 1606
Fragments arranged by size:
3364 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5842
AvaI C'yCGr_G
Cuts at: 1870 1943 2301 2526 3167 1870
Size: 73 358 225 641 4545
Fragments arranged by size:
4545 641 358 225 73
BamHI G'GATC_C
Cuts at: 2530 3172 3539 2530
Size: 642 367 4833
Fragments arranged by size:
4833 642 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2060 2229 3163 4618 618
Size: 325 217 627 273 169 934 1455 1842
Fragments arranged by size:
1842 1455 934 627 325 273 217 169
BanII G_rGCy'C
Cuts at: 1391 2245 2854 2876 3161 1391
Size: 854 609 22 285 4072
Fragments arranged by size:
4072 854 609 285 22
BbeI G_GCGC'C
Cuts at: 2064 2064
Size: 5842
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4871
BbvCI CC'TCA_GC
Cuts at: 2121 2121
Size: 5842
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4033 4067 1903
Size: 34 2096 34 3678
Fragments arranged by size:
3678 2096 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3734 4944 3734
Size: 1210 4632
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5842
BfrBI ATG'CAT
Cuts at: 2462 2462
Size: 5842
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4453 136
Size: 107 122 71 631 605 2781 1525
Fragments arranged by size:
2781 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5837 5837
Size: 5842
BlpI GC'TnA_GC
Cuts at: 3259 3259
Size: 5842
Bme1580I G_kGCm'C
Cuts at: 1790 2308 2854 3903 5059 1790
Size: 518 546 1049 1156 2573
Fragments arranged by size:
2573 1156 1049 546 518
BmgBI CAC'GTC
Cuts at: 1318 2309 1318
Size: 991 4851
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2879 3573 4531
Size: 416 53 83 399 24 1462 694 958
Cuts at: 4531 442
Size: 1753
Fragments arranged by size:
1753 1462 958 694 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5842
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4502 1239
Size: 3263 2579
Bpu10I CC'TnA_GC
Cuts at: 2121 2846 2121
Size: 725 5117
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2349 2593 3967 4832 5130 2349
Size: 244 1374 865 298 3061
Fragments arranged by size:
3061 1374 865 298 244
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2501 4505 882
Size: 617 1002 2004 2219
Fragments arranged by size:
2219 2004 1002 617
BsaAI yAC'GTr
Cuts at: 493 1204 3633 493
Size: 711 2429 2702
Fragments arranged by size:
2702 2429 711
BsaBI GATnn'nnATC
Cuts at: 5836 5836
Size: 5842
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2061 3649 4031 4666
Size: 53 83 186 936 528 1588 382 635
Cuts at: 4666 275
Size: 1451
Fragments arranged by size:
1588 1451 936 635 528 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4275 4673 4947 5094 5505 4275
Size: 398 274 147 411 4612
Fragments arranged by size:
4612 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5617
Fragments arranged by size:
5617 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5842
BseYI C'CCAG_C
Cuts at: 2028 2150 5045 5541 2028
Size: 122 2895 496 2329
Fragments arranged by size:
2895 2329 496 122
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2684 1163
Size: 573 948 4321
Fragments arranged by size:
4321 948 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3292 4053 5081 1540
Size: 275 186 1291 761 1028 2301
Fragments arranged by size:
2301 1291 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3903 3988 5059 3903
Size: 85 1071 4686
Fragments arranged by size:
4686 1071 85
BsmI GAATG_Cn'
Cuts at: 2587 3353 3446 2587
Size: 766 93 4983
Fragments arranged by size:
4983 766 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2245 2308 2854 2876 3161 3903 3988
Size: 399 455 63 546 22 285 742 85
Cuts at: 3988 5059 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 546 455 399 285 85
63 22
BspEI T'CCGG_A
Cuts at: 5505 5505
Size: 5842
BspHI T'CATG_A
Cuts at: 3731 5461 3731
Size: 1730 4112
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3197 844
Size: 2353 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3729 1394
Size: 429 1906 3507
Fragments arranged by size:
3507 1906 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2212 4337 4511 65
Size: 1183 964 2125 174 1396
Fragments arranged by size:
2125 1396 1183 964 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4486 4673 1700
Size: 110 2676 187 2869
Fragments arranged by size:
2869 2676 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5842
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5842
BssSI C'ACGA_G
Cuts at: 3902 4914 3902
Size: 1012 4830
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5842
BstBI TT'CG_AA
Cuts at: 4646 4646
Size: 5842
BstEII G'GTnAC_C
Cuts at: 1184 2032 1184
Size: 848 4994
BstYI r'GATC_y
Cuts at: 1972 2129 2530 3172 3539 3924 3941 5393 5479
Size: 157 401 642 367 385 17 1452 86
Cuts at: 5479 5491 5837 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 642 401 385 367 346 157
86 17 12
BstZ17I GTA'TAC
Cuts at: 2611 2611
Size: 5842
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4351
Fragments arranged by size:
4351 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5103
Fragments arranged by size:
5103 683 56
BtsI GCAGTG_nn'
Cuts at: 3358 4152 4172 3358
Size: 794 20 5028
Fragments arranged by size:
5028 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5842
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5807
DraI TTT'AAA
Cuts at: 3015 3146 3015
Size: 131 5711
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4849 809
Size: 507 3533 1802
Fragments arranged by size:
3533 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3102 3289 4178
Size: 54 1425 50 275 18 1272 187 889
Cuts at: 4178 5807 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1272 889 275 187 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3289 1537
Size: 275 1477 4090
Fragments arranged by size:
4090 1477 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3772 1353
Size: 2419 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4429 4803 4949 387
Size: 1064 138 2840 374 146 1280
Fragments arranged by size:
2840 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2277 2771 3905 4502 5289 5557 1239
Size: 393 645 494 1134 597 787 268 1524
Fragments arranged by size:
1524 1134 787 645 597 494 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2850 2850
Size: 5842
EcoRI G'AATT_C
Cuts at: 3151 3529 3151
Size: 378 5464
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2927 3017 2927
Size: 90 5752
FspI TGC'GCA
Cuts at: 4348 4348
Size: 5842
HaeII r_GCGC'y
Cuts at: 1678 2064 4989 1678
Size: 386 2925 2531
Fragments arranged by size:
2925 2531 386
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2114 2146 4483 4515 4557
Size: 32 135 32 542 32 2337 32 42
Cuts at: 4557 4589 1373
Size: 32 2626
Fragments arranged by size:
2626 2337 542 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3186 3432 669
Size: 988 281 1248 246 3079
Fragments arranged by size:
3079 1248 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5842
HpaI GTT'AAC
Cuts at: 3432 3432
Size: 5842
KasI G'GCGC_C
Cuts at: 2060 2060
Size: 5842
KpnI G_GTAC'C
Cuts at: 2233 3167 2233
Size: 934 4908
MfeI C'AATT_G
Cuts at: 2502 3441 2502
Size: 939 4903
MluI A'CGCG_T
Cuts at: 5718 5718
Size: 5842
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3070 4956 5140 1305
Size: 1765 1886 184 2007
Fragments arranged by size:
2007 1886 1765 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3104 10
Size: 54 1425 343 1272 2748
Fragments arranged by size:
2748 1425 1272 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3800 4159 4318 518
Size: 648 2634 359 159 2042
Fragments arranged by size:
2634 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2765 3258 3935 5083 5328 1606
Size: 1159 493 677 1148 245 2120
Fragments arranged by size:
2120 1159 1148 677 493 245
NarI GG'CG_CC
Cuts at: 2061 2061
Size: 5842
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4351
Fragments arranged by size:
4351 939 552
NdeI CA'TA_TG
Cuts at: 387 3120 387
Size: 2733 3109
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5842
NotI GC'GGCC_GC
Cuts at: 3289 3289
Size: 5842
NsiI A_TGCA'T
Cuts at: 2464 2464
Size: 5842
NspI r_CATG'y
Cuts at: 1132 1229 2725 3200 4745 1132
Size: 97 1496 475 1545 2229
Fragments arranged by size:
2229 1545 1496 475 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5842
PciI A'CATG_T
Cuts at: 4741 4741
Size: 5842
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5842
PmeI GTTT'AAAC
Cuts at: 3146 3146
Size: 5842
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3911 3943 5424 5456 3911
Size: 32 1481 32 4297
Fragments arranged by size:
4297 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5842
PsiI TTA'TAA
Cuts at: 3412 3412
Size: 5842
PspOMI G'GGCC_C
Cuts at: 2850 2850
Size: 5842
PstI C_TGCA'G
Cuts at: 830 3194 830
Size: 2364 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5842
SalI G'TCGA_C
Cuts at: 1936 3184 1936
Size: 1248 4594
SbfI CC_TGCA'GG
Cuts at: 3194 3194
Size: 5842
ScaI AGT'ACT
Cuts at: 4090 5802 4090
Size: 1712 4130
SfcI C'TryA_G
Cuts at: 826 1045 3190 4325 5006 5197 826
Size: 219 2145 1135 681 191 1471
Fragments arranged by size:
2145 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2062 2062
Size: 5842
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5842
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5842
SmaI CCC'GGG
Cuts at: 1872 2303 3169 1872
Size: 431 866 4545
Fragments arranged by size:
4545 866 431
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2364 2572 3946 4847 5109 820
Size: 197 926 421 208 1374 901 262 1553
Fragments arranged by size:
1553 1374 926 901 421 262 208 197
SnaBI TAC'GTA
Cuts at: 493 3633 493
Size: 3140 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5842
SphI G_CATG'C
Cuts at: 1132 3200 1132
Size: 2068 3774
SspI AAT'ATT
Cuts at: 5 52 3766 5
Size: 47 3714 2081
Fragments arranged by size:
3714 2081 47
StuI AGG'CCT
Cuts at: 1517 2043 1517
Size: 526 5316
StyI C'CwwG_G
Cuts at: 513 1065 2004 2237 3281 513
Size: 552 939 233 1044 3074
Fragments arranged by size:
3074 1044 939 552 233
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2020 2480 3869 3886 4039 4224 4669
Size: 1255 440 460 1389 17 153 185 445
Cuts at: 4669 325
Size: 1498
Fragments arranged by size:
1498 1389 1255 460 445 440 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4088 5800 96
Size: 274 80 33 51 1443 2111 1712 138
Fragments arranged by size:
2111 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5802 5834 5802
Size: 32 5810
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5842
XbaI T'CTAG_A
Cuts at: 3178 3178
Size: 5842
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5842
XmaI C'CCGG_G
Cuts at: 1870 2301 3167 1870
Size: 431 866 4545
Fragments arranged by size:
4545 866 431
XmnI GAAnn'nnTTC
Cuts at: 2907 2984 3971 2907
Size: 77 987 4778
Fragments arranged by size:
4778 987 77
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3650 276
Size: 53 83 186 936 2116 2468
Fragments arranged by size:
2468 2116 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BplI BsiWI BsmBI
BstXI Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI
NaeI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SrfI SwaI XcmI