Restriction map for
pFN21AB8811
(Circular) (Six-base) MAPSORT of: pFN21AB8811.seq Check: 3649 from: 1 to: 5791
pFN21AB8811 5791 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3601 278
Size: 53 83 186 936 2065 2468
Fragments arranged by size:
2468 2065 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2166 3134 1937
Size: 229 968 4594
Fragments arranged by size:
4594 968 229
Acc65I G'GTAC_C
Cuts at: 3112 3112
Size: 5791
AclI AA'CG_TT
Cuts at: 1563 3918 4291 1563
Size: 2355 373 3063
Fragments arranged by size:
3063 2355 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3089 3854 5238 5506 1632
Size: 1457 765 1384 268 1917
Fragments arranged by size:
1917 1457 1384 765 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5594
AflIII A'CryG_T
Cuts at: 1203 2016 4690 5667 1203
Size: 813 2674 977 1327
Fragments arranged by size:
2674 1327 977 813
AgeI A'CCGG_T
Cuts at: 4622 4622
Size: 5791
AhdI GACnn_n'nnGTC
Cuts at: 4520 4520
Size: 5791
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5791
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5791
AlwNI CAG_nnn'CTG
Cuts at: 3534 5106 3534
Size: 1572 4219
ApaLI G'TGCA_C
Cuts at: 3848 5004 3848
Size: 1156 4635
ApoI r'AATT_y
Cuts at: 1493 3100 3326 3478 5587 1493
Size: 1607 226 152 2109 1697
Fragments arranged by size:
2109 1697 1607 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4345 160
Size: 872 3313 1606
Fragments arranged by size:
3313 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5791
AvaI C'yCGr_G
Cuts at: 1870 1943 2264 3116 1870
Size: 73 321 852 4545
Fragments arranged by size:
4545 852 321 73
BamHI G'GATC_C
Cuts at: 2253 3121 3488 2253
Size: 868 367 4556
Fragments arranged by size:
4556 868 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2444 2455 2879 3112 4567
Size: 325 217 627 657 11 424 233 1455
Cuts at: 4567 618
Size: 1842
Fragments arranged by size:
1842 1455 657 627 424 325 233 217
11
BanII G_rGCy'C
Cuts at: 1391 2420 2478 3110 1391
Size: 1029 58 632 4072
Fragments arranged by size:
4072 1029 632 58
BbeI G_GCGC'C
Cuts at: 2448 2459 2883 2448
Size: 11 424 5356
Fragments arranged by size:
5356 424 11
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4820
BbvCI CC'TCA_GC
Cuts at: 2180 2684 2180
Size: 504 5287
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3982 4016 1903
Size: 34 2045 34 3678
Fragments arranged by size:
3678 2045 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3683 4893 3683
Size: 1210 4581
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5791
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2453 4402 136
Size: 107 122 71 631 605 781 1949 1525
Fragments arranged by size:
1949 1525 781 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5786 5786
Size: 5791
BlpI GC'TnA_GC
Cuts at: 3208 3208
Size: 5791
Bme1580I G_kGCm'C
Cuts at: 1790 3852 5008 1790
Size: 2062 1156 2573
Fragments arranged by size:
2573 2062 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5791
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3522 4480 442
Size: 416 53 83 399 24 2105 958 1753
Fragments arranged by size:
2105 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5791
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2993 4451 1239
Size: 1754 1458 2579
Fragments arranged by size:
2579 1754 1458
Bpu10I CC'TnA_GC
Cuts at: 2180 2684 2180
Size: 504 5287
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3916 4781 5079 3916
Size: 865 298 4628
Fragments arranged by size:
4628 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2478 2969 4454 882
Size: 617 979 491 1485 2219
Fragments arranged by size:
2219 1485 979 617 491
BsaAI yAC'GTr
Cuts at: 493 1204 2019 2035 2728 3582 493
Size: 711 815 16 693 854 2702
Fragments arranged by size:
2702 854 815 711 693 16
BsaBI GATnn'nnATC
Cuts at: 5785 5785
Size: 5791
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2163 2445 2456 2880
Size: 53 83 186 936 630 282 11 424
Cuts at: 2880 3598 3980 4615 275
Size: 718 382 635 1451
Fragments arranged by size:
1451 936 718 635 630 424 382 282
186 83 53 11
BsaWI w'CCGG_w
Cuts at: 4224 4622 4896 5043 5454 4224
Size: 398 274 147 411 4561
Fragments arranged by size:
4561 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5566
Fragments arranged by size:
5566 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2801 1186
Size: 1615 4176
BseYI C'CCAG_C
Cuts at: 2158 2440 3027 4994 5490 2158
Size: 282 587 1967 496 2459
Fragments arranged by size:
2459 1967 587 496 282
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2191 2222 2891 3083 1163
Size: 573 455 31 669 192 3871
Fragments arranged by size:
3871 669 573 455 192 31
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3241 4002 5030 1540
Size: 275 186 1240 761 1028 2301
Fragments arranged by size:
2301 1240 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2420 2478 2733 3852 3937 5008 2420
Size: 58 255 1119 85 1071 3203
Fragments arranged by size:
3203 1119 1071 255 85 58
BsmI GAATG_Cn'
Cuts at: 3302 3395 3302
Size: 93 5698
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2420 2478 2733 3110 3852 3937 5008
Size: 399 630 58 255 377 742 85 1071
Cuts at: 5008 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 630 399 377 255 85
58
BspEI T'CCGG_A
Cuts at: 5454 5454
Size: 5791
BspHI T'CATG_A
Cuts at: 3680 5410 3680
Size: 1730 4061
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2202 2922 3146 844
Size: 1358 720 224 3489
Fragments arranged by size:
3489 1358 720 224
BsrBI CCG'CTC
Cuts at: 1394 1823 3678 1394
Size: 429 1855 3507
Fragments arranged by size:
3507 1855 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4286 4460 65
Size: 1183 3038 174 1396
Fragments arranged by size:
3038 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4435 4622 1700
Size: 110 2625 187 2869
Fragments arranged by size:
2869 2625 187 110
BsrGI T'GTAC_A
Cuts at: 96 2199 96
Size: 2103 3688
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5791
BssSI C'ACGA_G
Cuts at: 3851 4863 3851
Size: 1012 4779
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2914 794
Size: 2120 3671
BstBI TT'CG_AA
Cuts at: 4595 4595
Size: 5791
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5791
BstXI CCAn_nnnn'nTGG
Cuts at: 2113 2440 2704 2758 2113
Size: 327 264 54 5146
Fragments arranged by size:
5146 327 264 54
BstYI r'GATC_y
Cuts at: 1972 2253 3121 3488 3873 3890 5342 5428 5440
Size: 281 868 367 385 17 1452 86 12
Cuts at: 5440 5786 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 868 385 367 346 281 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2257 2406 2421 2433 2523 2582
Size: 552 939 253 149 15 12 90 59
Cuts at: 2582 513
Size: 3722
Fragments arranged by size:
3722 939 552 253 149 90 59 15
12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2170 2302 2677 2904 487
Size: 683 56 944 132 375 227 3374
Fragments arranged by size:
3374 944 683 375 227 132 56
BtsI GCAGTG_nn'
Cuts at: 3307 4101 4121 3307
Size: 794 20 4977
Fragments arranged by size:
4977 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5791
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5756
DraI TTT'AAA
Cuts at: 3095 3095
Size: 5791
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4798 809
Size: 507 3482 1802
Fragments arranged by size:
3482 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2409 2895 2928
Size: 54 1425 50 275 18 579 486 33
Cuts at: 2928 2952 3033 3238 4127 5756 8
Size: 24 81 205 889 1629 43
Fragments arranged by size:
1629 1425 889 579 486 275 205 81
54 50 43 33 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 3238 1537
Size: 275 1426 4090
Fragments arranged by size:
4090 1426 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2483 2582 3721 1353
Size: 1130 99 1139 3423
Fragments arranged by size:
3423 1139 1130 99
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2052 2861 3030 4378 4752 4898
Size: 1064 138 463 809 169 1348 374 146
Cuts at: 4898 387
Size: 1280
Fragments arranged by size:
1348 1280 1064 809 463 374 169 146
138
EcoICRI GAG'CTC
Cuts at: 2418 2476 2418
Size: 58 5733
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2993 3089 3854 4451 5238 5506 1239
Size: 393 1361 96 765 597 787 268 1524
Fragments arranged by size:
1524 1361 787 765 597 393 268 96
EcoNI CCTnn'n_nnAGG
Cuts at: 2992 2992
Size: 5791
EcoO109I rG'GnC_Cy
Cuts at: 2268 2902 2268
Size: 634 5157
EcoRI G'AATT_C
Cuts at: 3100 3478 3100
Size: 378 5413
FspI TGC'GCA
Cuts at: 4297 4297
Size: 5791
HaeII r_GCGC'y
Cuts at: 1678 2448 2459 2591 2653 2751 2883 4938 1678
Size: 770 11 132 62 98 132 2055 2531
Fragments arranged by size:
2531 2055 770 132 132 98 62 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4432 4464 4506 4538 1373
Size: 32 135 32 2860 32 42 32 2626
Fragments arranged by size:
2860 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2872 3135 3381 669
Size: 988 281 934 263 246 3079
Fragments arranged by size:
3079 988 934 281 263 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5791
HpaI GTT'AAC
Cuts at: 3381 3381
Size: 5791
KasI G'GCGC_C
Cuts at: 2444 2455 2879 2444
Size: 11 424 5356
Fragments arranged by size:
5356 424 11
KpnI G_GTAC'C
Cuts at: 3116 3116
Size: 5791
MfeI C'AATT_G
Cuts at: 3390 3390
Size: 5791
MluI A'CGCG_T
Cuts at: 5667 5667
Size: 5791
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4905 5089 1305
Size: 3600 184 2007
Fragments arranged by size:
3600 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2897 2930 2954 10
Size: 54 1425 343 1065 33 24 2847
Fragments arranged by size:
2847 1425 1065 343 54 33 24
MslI CAynn'nnrTG
Cuts at: 518 1166 2090 2339 3749 4108 4267 518
Size: 648 924 249 1410 359 159 2042
Fragments arranged by size:
2042 1410 924 648 359 249 159
MspA1I CmG'CkG
Cuts at: 1606 2218 3027 3067 3207 3884 5032 5277 1606
Size: 612 809 40 140 677 1148 245 2120
Fragments arranged by size:
2120 1148 809 677 612 245 140 40
NarI GG'CG_CC
Cuts at: 2445 2456 2880 2445
Size: 11 424 5356
Fragments arranged by size:
5356 424 11
NcoI C'CATG_G
Cuts at: 513 1065 2004 2523 513
Size: 552 939 519 3781
Fragments arranged by size:
3781 939 552 519
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5791
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5791
NotI GC'GGCC_GC
Cuts at: 3238 3238
Size: 5791
NspI r_CATG'y
Cuts at: 1132 1229 3149 4694 1132
Size: 97 1920 1545 2229
Fragments arranged by size:
2229 1920 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2769 3049 1348
Size: 1421 280 4090
Fragments arranged by size:
4090 1421 280
PciI A'CATG_T
Cuts at: 4690 4690
Size: 5791
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2950 1108
Size: 1842 3949
PmeI GTTT'AAAC
Cuts at: 3095 3095
Size: 5791
PmlI CAC'GTG
Cuts at: 2019 2035 2728 2019
Size: 16 693 5082
Fragments arranged by size:
5082 693 16
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3860 3892 5373 5405 3860
Size: 32 1481 32 4246
Fragments arranged by size:
4246 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2268 2268
Size: 5791
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5791
PsiI TTA'TAA
Cuts at: 3361 3361
Size: 5791
PstI C_TGCA'G
Cuts at: 830 3143 830
Size: 2313 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5791
PvuII CAG'CTG
Cuts at: 3027 3067 3027
Size: 40 5751
SacI G_AGCT'C
Cuts at: 2420 2478 2420
Size: 58 5733
SalI G'TCGA_C
Cuts at: 1936 3133 1936
Size: 1197 4594
SapI GCTCTTCn'nnn_
Cuts at: 2483 2483
Size: 5791
SbfI CC_TGCA'GG
Cuts at: 3143 3143
Size: 5791
ScaI AGT'ACT
Cuts at: 4039 5751 4039
Size: 1712 4079
SexAI A'CCwGG_T
Cuts at: 3057 3057
Size: 5791
SfcI C'TryA_G
Cuts at: 826 1045 3139 4274 4955 5146 826
Size: 219 2094 1135 681 191 1471
Fragments arranged by size:
2094 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2446 2457 2881 2446
Size: 11 424 5356
Fragments arranged by size:
5356 424 11
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5791
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5791
SmaI CCC'GGG
Cuts at: 1872 2266 3118 1872
Size: 394 852 4545
Fragments arranged by size:
4545 852 394
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3895 4796 5058 820
Size: 197 926 1952 901 262 1553
Fragments arranged by size:
1952 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3582 493
Size: 3089 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5791
SphI G_CATG'C
Cuts at: 1132 3149 1132
Size: 2017 3774
SspI AAT'ATT
Cuts at: 5 52 3715 5
Size: 47 3663 2081
Fragments arranged by size:
3663 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5791
StyI C'CwwG_G
Cuts at: 513 1065 2004 2523 2898 3230 513
Size: 552 939 519 375 332 3074
Fragments arranged by size:
3074 939 552 519 375 332
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2212 2382 2524 3818 3835 3988 4173
Size: 1255 632 170 142 1294 17 153 185
Cuts at: 4173 4618 325
Size: 445 1498
Fragments arranged by size:
1498 1294 1255 632 445 185 170 153
142 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2199 4037 5749
Size: 274 80 33 51 1443 222 1838 1712
Cuts at: 5749 96
Size: 138
Fragments arranged by size:
1838 1712 1443 274 222 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5751 5783 5751
Size: 32 5759
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5791
XbaI T'CTAG_A
Cuts at: 3127 3127
Size: 5791
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2110 2110
Size: 5791
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5791
XmaI C'CCGG_G
Cuts at: 1870 2264 3116 1870
Size: 394 852 4545
Fragments arranged by size:
4545 852 394
XmnI GAAnn'nnTTC
Cuts at: 3920 3920
Size: 5791
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3599 276
Size: 53 83 186 936 2065 2468
Fragments arranged by size:
2468 2065 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PspOMI PspXI PsrI RsrII SacII SanDI SfiI SrfI
SwaI