Restriction map for
pFN21AB8809
(Circular) (Six-base) MAPSORT of: pFN21AB8809.seq Check: 2120 from: 1 to: 5785
pFN21AB8809 5785 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3595 278
Size: 53 83 186 936 2059 2468
Fragments arranged by size:
2468 2059 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3128 1937
Size: 1191 4594
Acc65I G'GTAC_C
Cuts at: 3106 3106
Size: 5785
AclI AA'CG_TT
Cuts at: 1563 3912 4285 1563
Size: 2349 373 3063
Fragments arranged by size:
3063 2349 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2807 3848 5232 5500 1632
Size: 1175 1041 1384 268 1917
Fragments arranged by size:
1917 1384 1175 1041 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5588
AflIII A'CryG_T
Cuts at: 1203 4684 5661 1203
Size: 3481 977 1327
Fragments arranged by size:
3481 1327 977
AgeI A'CCGG_T
Cuts at: 4616 4616
Size: 5785
AhdI GACnn_n'nnGTC
Cuts at: 4514 4514
Size: 5785
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5785
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5785
AlwNI CAG_nnn'CTG
Cuts at: 2498 3528 5100 2498
Size: 1030 1572 3183
Fragments arranged by size:
3183 1572 1030
ApaLI G'TGCA_C
Cuts at: 2423 3842 4998 2423
Size: 1419 1156 3210
Fragments arranged by size:
3210 1419 1156
ApoI r'AATT_y
Cuts at: 1493 2173 2933 3012 3094 3320 3472 5581 1493
Size: 680 760 79 82 226 152 2109 1697
Fragments arranged by size:
2109 1697 760 680 226 152 82 79
AseI AT'TA_AT
Cuts at: 160 1032 4339 160
Size: 872 3307 1606
Fragments arranged by size:
3307 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5785
AvaI C'yCGr_G
Cuts at: 1870 1943 3110 1870
Size: 73 1167 4545
Fragments arranged by size:
4545 1167 73
BamHI G'GATC_C
Cuts at: 3115 3482 3115
Size: 367 5418
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3106 4561 618
Size: 325 217 627 1319 1455 1842
Fragments arranged by size:
1842 1455 1319 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2124 3006 3104 1391
Size: 733 882 98 4072
Fragments arranged by size:
4072 882 733 98
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4814
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3976 4010 1903
Size: 34 2039 34 3678
Fragments arranged by size:
3678 2039 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2087 3677 4887 2087
Size: 1590 1210 2985
Fragments arranged by size:
2985 1590 1210
BclI T'GATC_A
Cuts at: 1548 2676 1548
Size: 1128 4657
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2864 4396 136
Size: 107 122 71 631 605 1192 1532 1525
Fragments arranged by size:
1532 1525 1192 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5780 5780
Size: 5785
BlpI GC'TnA_GC
Cuts at: 3202 3202
Size: 5785
Bme1580I G_kGCm'C
Cuts at: 1790 2427 3846 5002 1790
Size: 637 1419 1156 2573
Fragments arranged by size:
2573 1419 1156 637
BmgBI CAC'GTC
Cuts at: 1318 2595 1318
Size: 1277 4508
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3516 4474 442
Size: 416 53 83 399 24 2099 958 1753
Fragments arranged by size:
2099 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5785
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4445 1239
Size: 3206 2579
Bpu10I CC'TnA_GC
Cuts at: 2213 2213
Size: 5785
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2461 3910 4775 5073 2461
Size: 1449 865 298 3173
Fragments arranged by size:
3173 1449 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2792 4448 882
Size: 617 1293 1656 2219
Fragments arranged by size:
2219 1656 1293 617
BsaAI yAC'GTr
Cuts at: 493 1204 3576 493
Size: 711 2372 2702
Fragments arranged by size:
2702 2372 711
BsaBI GATnn'nnATC
Cuts at: 3066 5779 3066
Size: 2713 3072
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3592 3974 4609 275
Size: 53 83 186 936 2059 382 635 1451
Fragments arranged by size:
2059 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4218 4616 4890 5037 5448 4218
Size: 398 274 147 411 4555
Fragments arranged by size:
4555 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5560
Fragments arranged by size:
5560 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2996 1186
Size: 1810 3975
BseYI C'CCAG_C
Cuts at: 3032 4988 5484 3032
Size: 1956 496 3333
Fragments arranged by size:
3333 1956 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2815 1163
Size: 573 1079 4133
Fragments arranged by size:
4133 1079 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3235 3996 5024 1540
Size: 275 186 1234 761 1028 2301
Fragments arranged by size:
2301 1234 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2124 2427 2790 2988 3846 3931 5002 2124
Size: 303 363 198 858 85 1071 2907
Fragments arranged by size:
2907 1071 858 363 303 198 85
BsmI GAATG_Cn'
Cuts at: 3296 3389 3296
Size: 93 5692
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2124 2427 2790 2988 3006 3104 3846
Size: 399 334 303 363 198 18 98 742
Cuts at: 3846 3931 5002 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 363 334 303 198
98 85 18
BspEI T'CCGG_A
Cuts at: 5448 5448
Size: 5785
BspHI T'CATG_A
Cuts at: 2394 3064 3674 5404 2394
Size: 670 610 1730 2775
Fragments arranged by size:
2775 1730 670 610
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2244 2975 3140 844
Size: 1400 731 165 3489
Fragments arranged by size:
3489 1400 731 165
BsrBI CCG'CTC
Cuts at: 1394 1823 3672 1394
Size: 429 1849 3507
Fragments arranged by size:
3507 1849 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4280 4454 65
Size: 1183 3032 174 1396
Fragments arranged by size:
3032 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4429 4616 1700
Size: 110 2619 187 2869
Fragments arranged by size:
2869 2619 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5785
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5785
BssSI C'ACGA_G
Cuts at: 3845 4857 3845
Size: 1012 4773
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5785
BstBI TT'CG_AA
Cuts at: 4589 4589
Size: 5785
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5785
BstXI CCAn_nnnn'nTGG
Cuts at: 2999 2999
Size: 5785
BstYI r'GATC_y
Cuts at: 1972 2257 3115 3482 3867 3884 5336 5422 5434
Size: 285 858 367 385 17 1452 86 12
Cuts at: 5434 5780 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 858 385 367 346 285 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2647 2647
Size: 5785
BtgI C'CryG_G
Cuts at: 513 1065 2013 2742 3057 513
Size: 552 948 729 315 3241
Fragments arranged by size:
3241 948 729 552 315
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5046
Fragments arranged by size:
5046 683 56
BtsI GCAGTG_nn'
Cuts at: 2890 3301 4095 4115 2890
Size: 411 794 20 4560
Fragments arranged by size:
4560 794 411 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5785
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5750
DraI TTT'AAA
Cuts at: 3089 3089
Size: 5785
DraIII CAC_nnn'GTG
Cuts at: 2431 2595 2431
Size: 164 5621
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4792 809
Size: 507 3476 1802
Fragments arranged by size:
3476 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3232 4121 5750
Size: 54 1425 50 275 18 1402 889 1629
Cuts at: 5750 8
Size: 43
Fragments arranged by size:
1629 1425 1402 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3232 1537
Size: 275 1420 4090
Fragments arranged by size:
4090 1420 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2129 2270 2759 2795 2963 3715 1353
Size: 776 141 489 36 168 752 3423
Fragments arranged by size:
3423 776 752 489 168 141 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4372 4746 4892 387
Size: 1064 138 2783 374 146 1280
Fragments arranged by size:
2783 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2122 2122
Size: 5785
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2807 3848 4445 5232 5500 1239
Size: 393 1175 1041 597 787 268 1524
Fragments arranged by size:
1524 1175 1041 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2451 2685 2974 2451
Size: 234 289 5262
Fragments arranged by size:
5262 289 234
EcoRI G'AATT_C
Cuts at: 3094 3472 3094
Size: 378 5407
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2752 2752
Size: 5785
FspI TGC'GCA
Cuts at: 4291 4291
Size: 5785
HaeII r_GCGC'y
Cuts at: 1678 4932 1678
Size: 3254 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2625 2657 4426 4458 4500
Size: 32 135 32 1053 32 1769 32 42
Cuts at: 4500 4532 1373
Size: 32 2626
Fragments arranged by size:
2626 1769 1053 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3129 3375 669
Size: 988 281 1191 246 3079
Fragments arranged by size:
3079 1191 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5785
HpaI GTT'AAC
Cuts at: 3375 3375
Size: 5785
KpnI G_GTAC'C
Cuts at: 3110 3110
Size: 5785
MfeI C'AATT_G
Cuts at: 2418 3384 2418
Size: 966 4819
MluI A'CGCG_T
Cuts at: 5661 5661
Size: 5785
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4899 5083 1305
Size: 3594 184 2007
Fragments arranged by size:
3594 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3963
Fragments arranged by size:
3963 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2393 3743 4102 4261 518
Size: 648 1227 1350 359 159 2042
Fragments arranged by size:
2042 1350 1227 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3201 3878 5026 5271 1606
Size: 1595 677 1148 245 2120
Fragments arranged by size:
2120 1595 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 3057 513
Size: 552 1992 3241
Fragments arranged by size:
3241 1992 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5785
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5785
NotI GC'GGCC_GC
Cuts at: 3232 3232
Size: 5785
NspI r_CATG'y
Cuts at: 1132 1229 3143 4688 1132
Size: 97 1914 1545 2229
Fragments arranged by size:
2229 1914 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5785
PciI A'CATG_T
Cuts at: 4684 4684
Size: 5785
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2595 1108
Size: 1487 4298
PfoI T'CCnGG_A
Cuts at: 2345 2345
Size: 5785
PmeI GTTT'AAAC
Cuts at: 3089 3089
Size: 5785
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3854 3886 5367 5399 3854
Size: 32 1481 32 4240
Fragments arranged by size:
4240 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2451 2685 2451
Size: 234 5551
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5785
PsiI TTA'TAA
Cuts at: 3355 3355
Size: 5785
PstI C_TGCA'G
Cuts at: 830 2972 3137 830
Size: 2142 165 3478
Fragments arranged by size:
3478 2142 165
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5785
SacI G_AGCT'C
Cuts at: 2124 2124
Size: 5785
SalI G'TCGA_C
Cuts at: 1936 3127 1936
Size: 1191 4594
SapI GCTCTTCn'nnn_
Cuts at: 2129 2270 2795 2963 2129
Size: 141 525 168 4951
Fragments arranged by size:
4951 525 168 141
SbfI CC_TGCA'GG
Cuts at: 2972 3137 2972
Size: 165 5620
ScaI AGT'ACT
Cuts at: 2576 4033 5745 2576
Size: 1457 1712 2616
Fragments arranged by size:
2616 1712 1457
SfcI C'TryA_G
Cuts at: 826 1045 2951 2968 3133 4268 4949 5140 826
Size: 219 1906 17 165 1135 681 191 1471
Fragments arranged by size:
1906 1471 1135 681 219 191 165 17
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5785
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5785
SmaI CCC'GGG
Cuts at: 1872 3112 1872
Size: 1240 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2440 3889 4790 5052 820
Size: 197 926 497 1449 901 262 1553
Fragments arranged by size:
1553 1449 926 901 497 262 197
SnaBI TAC'GTA
Cuts at: 493 3576 493
Size: 3083 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5785
SphI G_CATG'C
Cuts at: 1132 3143 1132
Size: 2011 3774
SspI AAT'ATT
Cuts at: 5 52 3709 5
Size: 47 3657 2081
Fragments arranged by size:
3657 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5785
StyI C'CwwG_G
Cuts at: 513 1065 2145 2758 3057 3224 513
Size: 552 1080 613 299 167 3074
Fragments arranged by size:
3074 1080 613 552 299 167
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3812 3829 3982 4167 4612 325
Size: 1255 2232 17 153 185 445 1498
Fragments arranged by size:
2232 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2352 2574 2943
Size: 274 80 33 51 1443 375 222 369
Cuts at: 2943 4031 5743 96
Size: 1088 1712 138
Fragments arranged by size:
1712 1443 1088 375 369 274 222 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2163 2195 5745 5777 2163
Size: 32 3550 32 2171
Fragments arranged by size:
3550 2171 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5785
XbaI T'CTAG_A
Cuts at: 3121 3121
Size: 5785
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2315 2875 2315
Size: 560 5225
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5785
XmaI C'CCGG_G
Cuts at: 1870 3110 1870
Size: 1240 4545
XmnI GAAnn'nnTTC
Cuts at: 2820 3914 2820
Size: 1094 4691
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3593 276
Size: 53 83 186 936 2059 2468
Fragments arranged by size:
2468 2059 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII
SanDI SexAI SfiI SfoI SrfI SwaI