Restriction map for
pFN21AB8805
(Circular) (Six-base) MAPSORT of: pFN21AB8805.seq Check: 7235 from: 1 to: 5758
pFN21AB8805 5758 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3568 278
Size: 53 83 186 936 2032 2468
Fragments arranged by size:
2468 2032 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2703 3018 3101 1937
Size: 766 315 83 4594
Fragments arranged by size:
4594 766 315 83
Acc65I G'GTAC_C
Cuts at: 3079 3079
Size: 5758
AclI AA'CG_TT
Cuts at: 1563 3885 4258 1563
Size: 2322 373 3063
Fragments arranged by size:
3063 2322 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2899 3821 5205 5473 1632
Size: 1267 922 1384 268 1917
Fragments arranged by size:
1917 1384 1267 922 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5561
AflIII A'CryG_T
Cuts at: 1203 4657 5634 1203
Size: 3454 977 1327
Fragments arranged by size:
3454 1327 977
AgeI A'CCGG_T
Cuts at: 2222 4589 2222
Size: 2367 3391
AhdI GACnn_n'nnGTC
Cuts at: 4487 4487
Size: 5758
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5758
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2126 1184
Size: 942 4816
AlwNI CAG_nnn'CTG
Cuts at: 2089 3501 5073 2089
Size: 1412 1572 2774
Fragments arranged by size:
2774 1572 1412
ApaI G_GGCC'C
Cuts at: 2324 2559 2324
Size: 235 5523
ApaLI G'TGCA_C
Cuts at: 3815 4971 3815
Size: 1156 4602
ApoI r'AATT_y
Cuts at: 1493 3067 3293 3445 5554 1493
Size: 1574 226 152 2109 1697
Fragments arranged by size:
2109 1697 1574 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4312 160
Size: 872 3280 1606
Fragments arranged by size:
3280 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5758
AvaI C'yCGr_G
Cuts at: 1870 1943 2025 2215 2306 3083 1870
Size: 73 82 190 91 777 4545
Fragments arranged by size:
4545 777 190 91 82 73
BamHI G'GATC_C
Cuts at: 3088 3455 3088
Size: 367 5391
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2030 2219 2492 2535 2569
Size: 325 217 627 243 189 273 43 34
Cuts at: 2569 2674 2691 2846 3079 4534 618
Size: 105 17 155 233 1455 1842
Fragments arranged by size:
1842 1455 627 325 273 243 233 217
189 155 105 43 34 17
BanII G_rGCy'C
Cuts at: 1391 2313 2324 2559 2712 3077 1391
Size: 922 11 235 153 365 4072
Fragments arranged by size:
4072 922 365 235 153 11
BbeI G_GCGC'C
Cuts at: 2034 2496 2678 2695 2850 2034
Size: 462 182 17 155 4942
Fragments arranged by size:
4942 462 182 155 17
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4787
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3949 3983 1903
Size: 34 2012 34 3678
Fragments arranged by size:
3678 2012 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3650 4860 3650
Size: 1210 4548
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5758
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2550 2554 2689
Size: 107 122 71 631 605 878 4 135
Cuts at: 2689 4369 136
Size: 1680 1525
Fragments arranged by size:
1680 1525 878 631 605 135 122 107
71 4
BglII A'GATC_T
Cuts at: 5753 5753
Size: 5758
BlpI GC'TnA_GC
Cuts at: 2402 2711 3175 2402
Size: 309 464 4985
Fragments arranged by size:
4985 464 309
Bme1580I G_kGCm'C
Cuts at: 1790 2222 2324 2559 2672 3819 4975 1790
Size: 432 102 235 113 1147 1156 2573
Fragments arranged by size:
2573 1156 1147 432 235 113 102
BmgBI CAC'GTC
Cuts at: 1318 2614 1318
Size: 1296 4462
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2318 3489 4447
Size: 416 53 83 399 24 901 1171 958
Cuts at: 4447 442
Size: 1753
Fragments arranged by size:
1753 1171 958 901 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5758
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2032 2213 4418 1239
Size: 793 181 2205 2579
Fragments arranged by size:
2579 2205 793 181
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3883 4748 5046 3883
Size: 865 298 4595
Fragments arranged by size:
4595 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4421 882
Size: 617 2922 2219
Fragments arranged by size:
2922 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3549 493
Size: 711 2345 2702
Fragments arranged by size:
2702 2345 711
BsaBI GATnn'nnATC
Cuts at: 5752 5752
Size: 5758
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2031 2493 2675 2692
Size: 53 83 186 936 498 462 182 17
Cuts at: 2692 2847 3565 3947 4582 275
Size: 155 718 382 635 1451
Fragments arranged by size:
1451 936 718 635 498 462 382 186
182 155 83 53 17
BsaWI w'CCGG_w
Cuts at: 2222 4191 4589 4863 5010 5421 2222
Size: 1969 398 274 147 411 2559
Fragments arranged by size:
2559 1969 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2156 2180 2186 2210 1178
Size: 30 165 30 753 24 6 24 4726
Fragments arranged by size:
4726 753 165 30 30 24 24 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2161 2897 3068 1186
Size: 975 736 171 3876
Fragments arranged by size:
3876 975 736 171
BseYI C'CCAG_C
Cuts at: 2414 4961 5457 2414
Size: 2547 496 2715
Fragments arranged by size:
2715 2547 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5185
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2469 2581 2832 3208 3969 4997
Size: 275 186 468 112 251 376 761 1028
Cuts at: 4997 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 468 376 275 251 186
112
BsiHKAI G_wGCw'C
Cuts at: 2313 2712 3819 3904 4975 2313
Size: 399 1107 85 1071 3096
Fragments arranged by size:
3096 1107 1071 399 85
BsmI GAATG_Cn'
Cuts at: 3269 3362 3269
Size: 93 5665
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2222 2313 2324 2559 2672 2712 3077
Size: 399 432 91 11 235 113 40 365
Cuts at: 3077 3819 3904 4975 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 432 399 365 235 113
91 85 40 11
BspEI T'CCGG_A
Cuts at: 5421 5421
Size: 5758
BspHI T'CATG_A
Cuts at: 3647 5377 3647
Size: 1730 4028
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3113 844
Size: 2269 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2116 2406 2575 2583 3645 1394
Size: 429 293 290 169 8 1062 3507
Fragments arranged by size:
3507 1062 429 293 290 169 8
BsrDI GCAATG_nn'
Cuts at: 65 1248 4253 4427 65
Size: 1183 3005 174 1396
Fragments arranged by size:
3005 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2222 2367 2468 2694 2732 2996 4402
Size: 110 412 145 101 226 38 264 1406
Cuts at: 4402 4589 1700
Size: 187 2869
Fragments arranged by size:
2869 1406 412 264 226 187 145 110
101 38
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5758
BssHII G'CGCG_C
Cuts at: 1925 2418 2804 2884 1925
Size: 493 386 80 4799
Fragments arranged by size:
4799 493 386 80
BssSI C'ACGA_G
Cuts at: 2281 2926 3818 4830 2281
Size: 645 892 1012 3209
Fragments arranged by size:
3209 1012 892 645
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2089 794
Size: 1295 4463
BstBI TT'CG_AA
Cuts at: 4562 4562
Size: 5758
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5758
BstYI r'GATC_y
Cuts at: 1972 3088 3455 3840 3857 5309 5395 5407 5753
Size: 1116 367 385 17 1452 86 12 346
Cuts at: 5753 1972
Size: 1977
Fragments arranged by size:
1977 1452 1116 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2019 2244 2840 513
Size: 552 954 225 596 3431
Fragments arranged by size:
3431 954 596 552 225
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2089 2464 2482 2575 2944 487
Size: 683 56 863 375 18 93 369 3301
Fragments arranged by size:
3301 863 683 375 369 93 56 18
BtsI GCAGTG_nn'
Cuts at: 3274 4068 4088 3274
Size: 794 20 4944
Fragments arranged by size:
4944 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5758
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5723
DraI TTT'AAA
Cuts at: 3062 3062
Size: 5758
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2612 4765 809
Size: 507 1296 2153 1802
Fragments arranged by size:
2153 1802 1296 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2097 2251 2466
Size: 54 1425 50 275 18 267 154 215
Cuts at: 2466 2696 2829 2896 3205 4094 5723 8
Size: 230 133 67 309 889 1629 43
Fragments arranged by size:
1629 1425 889 309 275 267 230 215
154 133 67 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2466 2829 3205 1537
Size: 275 654 363 376 4090
Fragments arranged by size:
4090 654 376 363 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2123 2318 2516 3688 1353
Size: 770 195 198 1172 3423
Fragments arranged by size:
3423 1172 770 198 195
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2801 4345 4719 4865 387
Size: 1064 138 1212 1544 374 146 1280
Fragments arranged by size:
1544 1280 1212 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2311 2710 2311
Size: 399 5359
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2032 2213 2899 3821 4418 5205 5473
Size: 393 400 181 686 922 597 787 268
Cuts at: 5473 1239
Size: 1524
Fragments arranged by size:
1524 922 787 686 597 400 393 268
181
EcoO109I rG'GnC_Cy
Cuts at: 2320 2556 2320
Size: 236 5522
EcoRI G'AATT_C
Cuts at: 3067 3445 3067
Size: 378 5380
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2965 2965
Size: 5758
FseI GG_CCGG'CC
Cuts at: 2472 2472
Size: 5758
FspI TGC'GCA
Cuts at: 2768 4264 2768
Size: 1496 4262
FspAI rTGC'GCAy
Cuts at: 2768 2768
Size: 5758
HaeII r_GCGC'y
Cuts at: 1678 2019 2034 2071 2267 2374 2496 2545 2678
Size: 341 15 37 196 107 122 49 133
Cuts at: 2678 2695 2780 2850 4905 1678
Size: 17 85 70 2055 2531
Fragments arranged by size:
2531 2055 341 196 133 122 107 85
70 49 37 17 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2510 2542 4399 4431 4473
Size: 32 135 32 938 32 1857 32 42
Cuts at: 4473 4505 1373
Size: 32 2626
Fragments arranged by size:
2626 1857 938 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2437 2641 2860 3102 3348 669
Size: 988 281 499 204 219 242 246 3079
Fragments arranged by size:
3079 988 499 281 246 242 219 204
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5758
HpaI GTT'AAC
Cuts at: 3348 3348
Size: 5758
KasI G'GCGC_C
Cuts at: 2030 2492 2674 2691 2846 2030
Size: 462 182 17 155 4942
Fragments arranged by size:
4942 462 182 155 17
KpnI G_GTAC'C
Cuts at: 3083 3083
Size: 5758
MfeI C'AATT_G
Cuts at: 3357 3357
Size: 5758
MluI A'CGCG_T
Cuts at: 5634 5634
Size: 5758
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2199 4872 5056 1305
Size: 894 2673 184 2007
Fragments arranged by size:
2673 2007 894 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2253 10
Size: 54 1425 343 421 3515
Fragments arranged by size:
3515 1425 421 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3716 4075 4234 518
Size: 648 2550 359 159 2042
Fragments arranged by size:
2550 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2089 2246 2401 2635 2842 3174 3851 4999
Size: 483 157 155 234 207 332 677 1148
Cuts at: 4999 5244 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 483 332 245 234 207
157 155
NaeI GCC'GGC
Cuts at: 2470 2696 2470
Size: 226 5532
NarI GG'CG_CC
Cuts at: 2031 2493 2675 2692 2847 2031
Size: 462 182 17 155 4942
Fragments arranged by size:
4942 462 182 155 17
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5206
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5758
NgoMIV G'CCGG_C
Cuts at: 2468 2694 2468
Size: 226 5532
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5758
NotI GC'GGCC_GC
Cuts at: 3205 3205
Size: 5758
NspI r_CATG'y
Cuts at: 1132 1229 3116 4661 1132
Size: 97 1887 1545 2229
Fragments arranged by size:
2229 1887 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5758
PciI A'CATG_T
Cuts at: 4657 4657
Size: 5758
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 4856
PmeI GTTT'AAAC
Cuts at: 3062 3062
Size: 5758
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3827 3859 5340 5372 3827
Size: 32 1481 32 4213
Fragments arranged by size:
4213 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5758
PsiI TTA'TAA
Cuts at: 3328 3328
Size: 5758
PspOMI G'GGCC_C
Cuts at: 2320 2555 2320
Size: 235 5523
PstI C_TGCA'G
Cuts at: 830 3110 830
Size: 2280 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5758
PvuII CAG'CTG
Cuts at: 2401 2401
Size: 5758
SacI G_AGCT'C
Cuts at: 2313 2712 2313
Size: 399 5359
SacII CC_GC'GG
Cuts at: 2247 2843 2247
Size: 596 5162
SalI G'TCGA_C
Cuts at: 1936 3100 1936
Size: 1164 4594
SapI GCTCTTCn'nnn_
Cuts at: 2123 2318 2123
Size: 195 5563
SbfI CC_TGCA'GG
Cuts at: 3110 3110
Size: 5758
ScaI AGT'ACT
Cuts at: 4006 5718 4006
Size: 1712 4046
SexAI A'CCwGG_T
Cuts at: 2967 2967
Size: 5758
SfcI C'TryA_G
Cuts at: 826 1045 3106 4241 4922 5113 826
Size: 219 2061 1135 681 191 1471
Fragments arranged by size:
2061 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2032 2494 2676 2693 2848 2032
Size: 462 182 17 155 4942
Fragments arranged by size:
4942 462 182 155 17
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5758
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5758
SmaI CCC'GGG
Cuts at: 1872 2027 2217 3085 1872
Size: 155 190 868 4545
Fragments arranged by size:
4545 868 190 155
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3862 4763 5025 820
Size: 197 926 1919 901 262 1553
Fragments arranged by size:
1919 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3549 493
Size: 3056 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5758
SphI G_CATG'C
Cuts at: 1132 3116 1132
Size: 1984 3774
SspI AAT'ATT
Cuts at: 5 52 3682 5
Size: 47 3630 2081
Fragments arranged by size:
3630 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5758
StyI C'CwwG_G
Cuts at: 513 1065 3197 513
Size: 552 2132 3074
Fragments arranged by size:
3074 2132 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2618 2924 3785 3802 3955 4140 4585
Size: 1255 1038 306 861 17 153 185 445
Cuts at: 4585 325
Size: 1498
Fragments arranged by size:
1498 1255 1038 861 445 306 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4004 5716 96
Size: 274 80 33 51 1443 2027 1712 138
Fragments arranged by size:
2027 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5718 5750 5718
Size: 32 5726
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5758
XbaI T'CTAG_A
Cuts at: 3094 3094
Size: 5758
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5758
XmaI C'CCGG_G
Cuts at: 1870 2025 2215 3083 1870
Size: 155 190 868 4545
Fragments arranged by size:
4545 868 190 155
XmnI GAAnn'nnTTC
Cuts at: 2948 3887 2948
Size: 939 4819
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3566 276
Size: 53 83 186 936 2032 2468
Fragments arranged by size:
2468 2032 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FseI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoRV NruI NsiI PacI PfoI PmlI PpuMI
PspXI PsrI RsrII SanDI SfiI SrfI SwaI XcmI