Restriction map for
pFN21AB8802
(Circular) (Six-base) MAPSORT of: pFN21AB8802.seq Check: 1315 from: 1 to: 5743
pFN21AB8802 5743 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3553 278
Size: 53 83 186 936 2017 2468
Fragments arranged by size:
2468 2017 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2239 3086 1937
Size: 302 847 4594
Fragments arranged by size:
4594 847 302
Acc65I G'GTAC_C
Cuts at: 3064 3064
Size: 5743
AclI AA'CG_TT
Cuts at: 1563 3870 4243 1563
Size: 2307 373 3063
Fragments arranged by size:
3063 2307 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3806 5190 5458 1632
Size: 2174 1384 268 1917
Fragments arranged by size:
2174 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5546
AflIII A'CryG_T
Cuts at: 1203 4642 5619 1203
Size: 3439 977 1327
Fragments arranged by size:
3439 1327 977
AgeI A'CCGG_T
Cuts at: 4574 4574
Size: 5743
AhdI GACnn_n'nnGTC
Cuts at: 4472 4472
Size: 5743
AleI CACnn'nnGTG
Cuts at: 1166 2393 1166
Size: 1227 4516
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5743
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2369 2401 2369
Size: 32 5711
AlwNI CAG_nnn'CTG
Cuts at: 2629 3486 5058 2629
Size: 857 1572 3314
Fragments arranged by size:
3314 1572 857
ApaI G_GGCC'C
Cuts at: 2428 2782 2428
Size: 354 5389
ApaLI G'TGCA_C
Cuts at: 3800 4956 3800
Size: 1156 4587
ApoI r'AATT_y
Cuts at: 1493 2864 3052 3278 3430 5539 1493
Size: 1371 188 226 152 2109 1697
Fragments arranged by size:
2109 1697 1371 226 188 152
AseI AT'TA_AT
Cuts at: 160 1032 4297 160
Size: 872 3265 1606
Fragments arranged by size:
3265 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5743
AvaI C'yCGr_G
Cuts at: 1870 1943 2391 2749 3068 1870
Size: 73 448 358 319 4545
Fragments arranged by size:
4545 448 358 319 73
BamHI G'GATC_C
Cuts at: 2824 3019 3073 3440 2824
Size: 195 54 367 5127
Fragments arranged by size:
5127 367 195 54
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2743 3064 4519 618
Size: 325 217 627 956 321 1455 1842
Fragments arranged by size:
1842 1455 956 627 325 321 217
BanII G_rGCy'C
Cuts at: 1391 2280 2428 2782 3062 1391
Size: 889 148 354 280 4072
Fragments arranged by size:
4072 889 354 280 148
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2144 928
Size: 971 245 4527
Fragments arranged by size:
4527 971 245
BbvCI CC'TCA_GC
Cuts at: 2795 2795
Size: 5743
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2089 2123 3934 3968 1903
Size: 34 152 34 1811 34 3678
Fragments arranged by size:
3678 1811 152 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3635 4845 3635
Size: 1210 4533
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5743
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2639 4354 136
Size: 107 122 71 631 605 967 1715 1525
Fragments arranged by size:
1715 1525 967 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5738 5738
Size: 5743
BlpI GC'TnA_GC
Cuts at: 3160 3160
Size: 5743
Bme1580I G_kGCm'C
Cuts at: 1790 2428 2748 2782 3804 4960 1790
Size: 638 320 34 1022 1156 2573
Fragments arranged by size:
2573 1156 1022 638 320 34
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5743
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2254 3474 4432
Size: 416 53 83 399 24 837 1220 958
Cuts at: 4432 442
Size: 1753
Fragments arranged by size:
1753 1220 958 837 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5743
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2294 2294
Size: 5743
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2480 2573 4403 1239
Size: 1241 93 1830 2579
Fragments arranged by size:
2579 1830 1241 93
Bpu10I CC'TnA_GC
Cuts at: 2795 2795
Size: 5743
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3868 4733 5031 3868
Size: 865 298 4580
Fragments arranged by size:
4580 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4406 882
Size: 617 2907 2219
Fragments arranged by size:
2907 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3534 493
Size: 711 2330 2702
Fragments arranged by size:
2702 2330 711
BsaBI GATnn'nnATC
Cuts at: 5737 5737
Size: 5743
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3550 3932 4567 275
Size: 53 83 186 936 2017 382 635 1451
Fragments arranged by size:
2017 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4176 4574 4848 4995 5406 4176
Size: 398 274 147 411 4513
Fragments arranged by size:
4513 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2030 2060 2369 2399 1178
Size: 30 165 30 627 30 309 30 4522
Fragments arranged by size:
4522 627 309 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5743
BseYI C'CCAG_C
Cuts at: 2086 2150 2708 4946 5442 2086
Size: 64 558 2238 496 2387
Fragments arranged by size:
2387 2238 558 496 64
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2398 2768 1163
Size: 573 662 370 4138
Fragments arranged by size:
4138 662 573 370
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2490 2962 3193 3954 4982 1540
Size: 275 186 489 472 231 761 1028 2301
Fragments arranged by size:
2301 1028 761 489 472 275 231 186
BsiHKAI G_wGCw'C
Cuts at: 3804 3889 4960 3804
Size: 85 1071 4587
Fragments arranged by size:
4587 1071 85
BsmI GAATG_Cn'
Cuts at: 3254 3347 3254
Size: 93 5650
BsmBI CGTCTCn'nnnn_
Cuts at: 2477 2497 2477
Size: 20 5723
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2280 2428 2748 2782 3062 3804 3889
Size: 399 490 148 320 34 280 742 85
Cuts at: 3889 4960 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 490 399 320 280 148
85 34
BspEI T'CCGG_A
Cuts at: 5406 5406
Size: 5743
BspHI T'CATG_A
Cuts at: 3632 5362 3632
Size: 1730 4013
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2712 3098 844
Size: 1868 386 3489
Fragments arranged by size:
3489 1868 386
BsrBI CCG'CTC
Cuts at: 1394 1823 3630 1394
Size: 429 1807 3507
Fragments arranged by size:
3507 1807 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2107 3020 4238 4412 65
Size: 1183 859 913 1218 174 1396
Fragments arranged by size:
1396 1218 1183 913 859 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4387 4574 1700
Size: 110 2577 187 2869
Fragments arranged by size:
2869 2577 187 110
BsrGI T'GTAC_A
Cuts at: 96 2007 96
Size: 1911 3832
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5743
BssSI C'ACGA_G
Cuts at: 3803 4815 3803
Size: 1012 4731
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2412 2970 794
Size: 1618 558 3567
Fragments arranged by size:
3567 1618 558
BstBI TT'CG_AA
Cuts at: 2951 4547 2951
Size: 1596 4147
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5743
BstXI CCAn_nnnn'nTGG
Cuts at: 2395 3008 2395
Size: 613 5130
BstYI r'GATC_y
Cuts at: 1972 2824 3019 3073 3440 3825 3842 5294 5380
Size: 852 195 54 367 385 17 1452 86
Cuts at: 5380 5392 5738 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 852 385 367 346 195 86
54 17 12
Bsu36I CC'TnA_GG
Cuts at: 2612 2612
Size: 5743
BtgI C'CryG_G
Cuts at: 513 1065 2178 2773 513
Size: 552 1113 595 3483
Fragments arranged by size:
3483 1113 595 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5004
Fragments arranged by size:
5004 683 56
BtsI GCAGTG_nn'
Cuts at: 3259 4053 4073 3259
Size: 794 20 4929
Fragments arranged by size:
4929 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5743
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5708
DraI TTT'AAA
Cuts at: 3047 3047
Size: 5743
DraIII CAC_nnn'GTG
Cuts at: 2374 2421 2374
Size: 47 5696
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4750 809
Size: 507 3434 1802
Fragments arranged by size:
3434 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2175 2289 2487
Size: 54 1425 50 275 18 345 114 198
Cuts at: 2487 2520 2631 3190 4079 5708 8
Size: 33 111 559 889 1629 43
Fragments arranged by size:
1629 1425 889 559 345 275 198 114
111 54 50 43 33 18
EagI C'GGCC_G
Cuts at: 1537 1812 2487 3190 1537
Size: 275 675 703 4090
Fragments arranged by size:
4090 703 675 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2285 2931 3673 1353
Size: 932 646 742 3423
Fragments arranged by size:
3423 932 742 646
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2632 4330 4704 4850 387
Size: 1064 138 1043 1698 374 146 1280
Fragments arranged by size:
1698 1280 1064 1043 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2480 2573 3806 4403 5190 5458 1239
Size: 393 848 93 1233 597 787 268 1524
Fragments arranged by size:
1524 1233 848 787 597 393 268 93
EcoO109I rG'GnC_Cy
Cuts at: 2425 2778 2779 2425
Size: 353 1 5389
Fragments arranged by size:
5389 353 1
EcoRI G'AATT_C
Cuts at: 3052 3430 3052
Size: 378 5365
FspI TGC'GCA
Cuts at: 4249 4249
Size: 5743
HaeII r_GCGC'y
Cuts at: 1678 4890 1678
Size: 3212 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2030 2062 4384 4416 4458
Size: 32 135 32 458 32 2322 32 42
Cuts at: 4458 4490 1373
Size: 32 2626
Fragments arranged by size:
2626 2322 458 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3087 3333 669
Size: 988 281 1149 246 3079
Fragments arranged by size:
3079 1149 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5743
HpaI GTT'AAC
Cuts at: 3333 3333
Size: 5743
KpnI G_GTAC'C
Cuts at: 3068 3068
Size: 5743
MfeI C'AATT_G
Cuts at: 2682 3342 2682
Size: 660 5083
MluI A'CGCG_T
Cuts at: 5619 5619
Size: 5743
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4857 5041 1305
Size: 3552 184 2007
Fragments arranged by size:
3552 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2291 2633 10
Size: 54 1425 343 459 342 3120
Fragments arranged by size:
3120 1425 459 343 342 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2393 2672 2675 2807 2978 3701 4060
Size: 648 1227 279 3 132 171 723 359
Cuts at: 4060 4219 518
Size: 159 2042
Fragments arranged by size:
2042 1227 723 648 359 279 171 159
132 3
MspA1I CmG'CkG
Cuts at: 1606 2065 2551 2629 3159 3836 4984 5229 1606
Size: 459 486 78 530 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 530 486 459 245 78
NcoI C'CATG_G
Cuts at: 513 1065 2773 513
Size: 552 1708 3483
Fragments arranged by size:
3483 1708 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5743
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5743
NotI GC'GGCC_GC
Cuts at: 3190 3190
Size: 5743
NspI r_CATG'y
Cuts at: 1132 1229 2700 2821 3101 4646 1132
Size: 97 1471 121 280 1545 2229
Fragments arranged by size:
2229 1545 1471 280 121 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5743
PciI A'CATG_T
Cuts at: 4642 4642
Size: 5743
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2529 1108
Size: 1421 4322
PmeI GTTT'AAAC
Cuts at: 3047 3047
Size: 5743
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2369 2401 3812 3844 5325 5357 2369
Size: 32 1411 32 1481 32 2755
Fragments arranged by size:
2755 1481 1411 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2489 1613
Size: 876 4867
PsiI TTA'TAA
Cuts at: 3313 3313
Size: 5743
PspOMI G'GGCC_C
Cuts at: 2424 2778 2424
Size: 354 5389
PspXI vC'TCGA_Gb
Cuts at: 2749 2749
Size: 5743
PstI C_TGCA'G
Cuts at: 830 2891 3095 830
Size: 2061 204 3478
Fragments arranged by size:
3478 2061 204
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5743
PvuII CAG'CTG
Cuts at: 2551 2629 2551
Size: 78 5665
SalI G'TCGA_C
Cuts at: 1936 3085 1936
Size: 1149 4594
SapI GCTCTTCn'nnn_
Cuts at: 2285 2285
Size: 5743
SbfI CC_TGCA'GG
Cuts at: 3095 3095
Size: 5743
ScaI AGT'ACT
Cuts at: 3991 5703 3991
Size: 1712 4031
SfcI C'TryA_G
Cuts at: 826 1045 2887 3091 4226 4907 5098 826
Size: 219 1842 204 1135 681 191 1471
Fragments arranged by size:
1842 1471 1135 681 219 204 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5743
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5743
SmaI CCC'GGG
Cuts at: 1872 2393 3070 1872
Size: 521 677 4545
Fragments arranged by size:
4545 677 521
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2749 3847 4748 5010 820
Size: 197 926 806 1098 901 262 1553
Fragments arranged by size:
1553 1098 926 901 806 262 197
SnaBI TAC'GTA
Cuts at: 493 3534 493
Size: 3041 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5743
SphI G_CATG'C
Cuts at: 1132 2821 3101 1132
Size: 1689 280 3774
Fragments arranged by size:
3774 1689 280
SspI AAT'ATT
Cuts at: 5 52 3667 5
Size: 47 3615 2081
Fragments arranged by size:
3615 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5743
StyI C'CwwG_G
Cuts at: 513 1065 2773 3182 513
Size: 552 1708 409 3074
Fragments arranged by size:
3074 1708 552 409
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2948 3770 3787 3940 4125 4570 325
Size: 1255 1368 822 17 153 185 445 1498
Fragments arranged by size:
1498 1368 1255 822 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2007 3989 5701
Size: 274 80 33 51 1443 30 1982 1712
Cuts at: 5701 96
Size: 138
Fragments arranged by size:
1982 1712 1443 274 138 80 51 33
30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5703 5735 5703
Size: 32 5711
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5743
XbaI T'CTAG_A
Cuts at: 3079 3079
Size: 5743
XhoI C'TCGA_G
Cuts at: 1943 2749 1943
Size: 806 4937
XmaI C'CCGG_G
Cuts at: 1870 2391 3068 1870
Size: 521 677 4545
Fragments arranged by size:
4545 677 521
XmnI GAAnn'nnTTC
Cuts at: 3872 3872
Size: 5743
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3551 276
Size: 53 83 186 936 2017 2468
Fragments arranged by size:
2468 2017 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BfrBI BsiWI
BstZ17I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PsrI RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI XcmI