Restriction map for
pFN21AB8800
(Circular) (Six-base) MAPSORT of: pFN21AB8800.seq Check: 2737 from: 1 to: 5731
pFN21AB8800 5731 bp
With 182 enzymes: SgfI *
March 28, 2012 14:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2375 2375
Size: 5731
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3541 278
Size: 53 83 186 936 2005 2468
Fragments arranged by size:
2468 2005 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3074 1937
Size: 1137 4594
Acc65I G'GTAC_C
Cuts at: 3052 3052
Size: 5731
AclI AA'CG_TT
Cuts at: 1563 3858 4231 1563
Size: 2295 373 3063
Fragments arranged by size:
3063 2295 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2903 3794 5178 5446 1632
Size: 1271 891 1384 268 1917
Fragments arranged by size:
1917 1384 1271 891 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5534
AflIII A'CryG_T
Cuts at: 1203 2346 4630 5607 1203
Size: 1143 2284 977 1327
Fragments arranged by size:
2284 1327 1143 977
AgeI A'CCGG_T
Cuts at: 4562 4562
Size: 5731
AhdI GACnn_n'nnGTC
Cuts at: 4460 4460
Size: 5731
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5731
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5731
AlwNI CAG_nnn'CTG
Cuts at: 2197 3474 5046 2197
Size: 1277 1572 2882
Fragments arranged by size:
2882 1572 1277
ApaLI G'TGCA_C
Cuts at: 3788 4944 3788
Size: 1156 4575
ApoI r'AATT_y
Cuts at: 1493 3040 3266 3418 5527 1493
Size: 1547 226 152 2109 1697
Fragments arranged by size:
2109 1697 1547 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4285 160
Size: 872 3253 1606
Fragments arranged by size:
3253 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5731
AvaI C'yCGr_G
Cuts at: 1870 1943 3056 1870
Size: 73 1113 4545
Fragments arranged by size:
4545 1113 73
BamHI G'GATC_C
Cuts at: 3061 3428 3061
Size: 367 5364
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3052 4507 618
Size: 325 217 627 1265 1455 1842
Fragments arranged by size:
1842 1455 1265 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3050 1391
Size: 1659 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2034 928
Size: 971 135 4625
Fragments arranged by size:
4625 971 135
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3922 3956 1903
Size: 34 1985 34 3678
Fragments arranged by size:
3678 1985 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3623 4833 3623
Size: 1210 4521
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5731
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2322 2411 4342
Size: 107 122 71 631 605 650 89 1931
Cuts at: 4342 136
Size: 1525
Fragments arranged by size:
1931 1525 650 631 605 122 107 89
71
BglII A'GATC_T
Cuts at: 2687 5726 2687
Size: 3039 2692
BlpI GC'TnA_GC
Cuts at: 3148 3148
Size: 5731
Bme1580I G_kGCm'C
Cuts at: 1790 3792 4948 1790
Size: 2002 1156 2573
Fragments arranged by size:
2573 2002 1156
BmgBI CAC'GTC
Cuts at: 1318 2349 1318
Size: 1031 4700
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2330 2606 2785
Size: 416 53 83 399 24 913 276 179
Cuts at: 2785 3462 4420 442
Size: 677 958 1753
Fragments arranged by size:
1753 958 913 677 416 399 276 179
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5731
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2688 4391 1239
Size: 1449 1703 2579
Fragments arranged by size:
2579 1703 1449
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3856 4721 5019 3856
Size: 865 298 4568
Fragments arranged by size:
4568 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2950 4394 882
Size: 617 1451 1444 2219
Fragments arranged by size:
2219 1451 1444 617
BsaAI yAC'GTr
Cuts at: 493 1204 3522 493
Size: 711 2318 2702
Fragments arranged by size:
2702 2318 711
BsaBI GATnn'nnATC
Cuts at: 5725 5725
Size: 5731
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3538 3920 4555 275
Size: 53 83 186 936 2005 382 635 1451
Fragments arranged by size:
2005 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4164 4562 4836 4983 5394 4164
Size: 398 274 147 411 4501
Fragments arranged by size:
4501 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5506
Fragments arranged by size:
5506 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2081 1186
Size: 895 4836
BseYI C'CCAG_C
Cuts at: 4934 5430 4934
Size: 496 5235
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2233 1163
Size: 573 497 4661
Fragments arranged by size:
4661 573 497
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3181 3942 4970 1540
Size: 275 186 1180 761 1028 2301
Fragments arranged by size:
2301 1180 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2865 3792 3877 4948 2865
Size: 927 85 1071 3648
Fragments arranged by size:
3648 1071 927 85
BsmI GAATG_Cn'
Cuts at: 3242 3335 3242
Size: 93 5638
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2865 3050 3792 3877 4948 1391
Size: 399 1075 185 742 85 1071 2174
Fragments arranged by size:
2174 1075 1071 742 399 185 85
BspEI T'CCGG_A
Cuts at: 5394 5394
Size: 5731
BspHI T'CATG_A
Cuts at: 2751 3620 5350 2751
Size: 869 1730 3132
Fragments arranged by size:
3132 1730 869
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2375 3086 844
Size: 1531 711 3489
Fragments arranged by size:
3489 1531 711
BsrBI CCG'CTC
Cuts at: 1394 1823 3618 1394
Size: 429 1795 3507
Fragments arranged by size:
3507 1795 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2521 4226 4400 65
Size: 1183 1273 1705 174 1396
Fragments arranged by size:
1705 1396 1273 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4375 4562 1700
Size: 110 2565 187 2869
Fragments arranged by size:
2869 2565 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5731
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5731
BssSI C'ACGA_G
Cuts at: 3791 4803 3791
Size: 1012 4719
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5731
BstBI TT'CG_AA
Cuts at: 2890 4535 2890
Size: 1645 4086
BstEII G'GTnAC_C
Cuts at: 1184 2452 2546 1184
Size: 1268 94 4369
Fragments arranged by size:
4369 1268 94
BstYI r'GATC_y
Cuts at: 1972 2687 3061 3428 3813 3830 5282 5368 5380
Size: 715 374 367 385 17 1452 86 12
Cuts at: 5380 5726 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 715 385 374 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2840 2840
Size: 5731
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4240
Fragments arranged by size:
4240 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2815 487
Size: 683 56 1589 3403
Fragments arranged by size:
3403 1589 683 56
BtsI GCAGTG_nn'
Cuts at: 2612 2654 3247 4041 4061 2612
Size: 42 593 794 20 4282
Fragments arranged by size:
4282 794 593 42 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5731
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5696
DraI TTT'AAA
Cuts at: 3035 3035
Size: 5731
DraIII CAC_nnn'GTG
Cuts at: 3004 3004
Size: 5731
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4738 809
Size: 507 3422 1802
Fragments arranged by size:
3422 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2296 3178 4067
Size: 54 1425 50 275 18 466 882 889
Cuts at: 4067 5696 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 882 466 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3178 1537
Size: 275 1366 4090
Fragments arranged by size:
4090 1366 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3661 1353
Size: 2308 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4318 4692 4838 387
Size: 1064 138 2729 374 146 1280
Fragments arranged by size:
2729 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2688 2903 3794 4391 5178 5446 1239
Size: 393 1056 215 891 597 787 268 1524
Fragments arranged by size:
1524 1056 891 787 597 393 268 215
EcoO109I rG'GnC_Cy
Cuts at: 2844 2844
Size: 5731
EcoRI G'AATT_C
Cuts at: 3040 3418 3040
Size: 378 5353
EcoRV GAT'ATC
Cuts at: 2478 2478
Size: 5731
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2719 2719
Size: 5731
FspI TGC'GCA
Cuts at: 4237 4237
Size: 5731
HaeII r_GCGC'y
Cuts at: 1678 4878 1678
Size: 3200 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4372 4404 4446 4478 1373
Size: 32 135 32 2800 32 42 32 2626
Fragments arranged by size:
2800 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3075 3321 669
Size: 988 281 1137 246 3079
Fragments arranged by size:
3079 1137 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5731
HpaI GTT'AAC
Cuts at: 3321 3321
Size: 5731
KpnI G_GTAC'C
Cuts at: 3056 3056
Size: 5731
MfeI C'AATT_G
Cuts at: 3330 3330
Size: 5731
MluI A'CGCG_T
Cuts at: 5607 5607
Size: 5731
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4845 5029 1305
Size: 3540 184 2007
Fragments arranged by size:
3540 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2298 10
Size: 54 1425 343 466 3443
Fragments arranged by size:
3443 1425 466 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2351 2375 2393 2471 2588 2813 2816
Size: 648 1185 24 18 78 117 225 3
Cuts at: 2816 2953 3017 3689 4048 4207 518
Size: 137 64 672 359 159 2042
Fragments arranged by size:
2042 1185 672 648 359 225 159 137
117 78 64 24 18 3
MspA1I CmG'CkG
Cuts at: 1606 3147 3824 4972 5217 1606
Size: 1541 677 1148 245 2120
Fragments arranged by size:
2120 1541 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4240
Fragments arranged by size:
4240 939 552
NdeI CA'TA_TG
Cuts at: 387 2540 387
Size: 2153 3578
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5731
NotI GC'GGCC_GC
Cuts at: 3178 3178
Size: 5731
NspI r_CATG'y
Cuts at: 1132 1229 2116 3022 3089 4634 1132
Size: 97 887 906 67 1545 2229
Fragments arranged by size:
2229 1545 906 887 97 67
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5731
PciI A'CATG_T
Cuts at: 4630 4630
Size: 5731
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3004 1108
Size: 1896 3835
PmeI GTTT'AAAC
Cuts at: 3035 3035
Size: 5731
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3800 3832 5313 5345 3800
Size: 32 1481 32 4186
Fragments arranged by size:
4186 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5731
PsiI TTA'TAA
Cuts at: 3301 3301
Size: 5731
PstI C_TGCA'G
Cuts at: 830 2088 3083 830
Size: 1258 995 3478
Fragments arranged by size:
3478 1258 995
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5731
SalI G'TCGA_C
Cuts at: 1936 3073 1936
Size: 1137 4594
SbfI CC_TGCA'GG
Cuts at: 3083 3083
Size: 5731
ScaI AGT'ACT
Cuts at: 3979 5691 3979
Size: 1712 4019
SexAI A'CCwGG_T
Cuts at: 2118 2235 2118
Size: 117 5614
SfcI C'TryA_G
Cuts at: 826 1045 2084 3079 4214 4895 5086 826
Size: 219 1039 995 1135 681 191 1471
Fragments arranged by size:
1471 1135 1039 995 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2411 2411
Size: 5731
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5731
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5731
SmaI CCC'GGG
Cuts at: 1872 3058 1872
Size: 1186 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3835 4736 4998 820
Size: 197 926 1892 901 262 1553
Fragments arranged by size:
1892 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3522 493
Size: 3029 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5731
SphI G_CATG'C
Cuts at: 1132 3089 1132
Size: 1957 3774
SspI AAT'ATT
Cuts at: 5 52 3655 5
Size: 47 3603 2081
Fragments arranged by size:
3603 2081 47
StuI AGG'CCT
Cuts at: 1517 2414 1517
Size: 897 4834
StyI C'CwwG_G
Cuts at: 513 1065 2004 2409 2938 3170 513
Size: 552 939 405 529 232 3074
Fragments arranged by size:
3074 939 552 529 405 232
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2278 2461 2600 3758 3775 3928 4113
Size: 1255 698 183 139 1158 17 153 185
Cuts at: 4113 4558 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1158 698 445 185 183 153
139 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3977 5689 96
Size: 274 80 33 51 1443 2000 1712 138
Fragments arranged by size:
2000 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5691 5723 5691
Size: 32 5699
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5731
XbaI T'CTAG_A
Cuts at: 3067 3067
Size: 5731
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2030 2507 2030
Size: 477 5254
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5731
XmaI C'CCGG_G
Cuts at: 1870 3056 1870
Size: 1186 4545
XmnI GAAnn'nnTTC
Cuts at: 3860 3860
Size: 5731
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3539 276
Size: 53 83 186 936 2005 2468
Fragments arranged by size:
2468 2005 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SalI SbfI ScaI SexAI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI
EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SfoI SrfI
SwaI