Restriction map for
pFN21AB8794
(Circular) (Six-base) MAPSORT of: pFN21AB8794.seq Check: 1632 from: 1 to: 5695
pFN21AB8794 5695 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2709 2709
Size: 5695
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3505 278
Size: 53 83 186 936 1969 2468
Fragments arranged by size:
2468 1969 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2383 2400 2799 3038 1937
Size: 446 17 399 239 4594
Fragments arranged by size:
4594 446 399 239 17
Acc65I G'GTAC_C
Cuts at: 3016 3016
Size: 5695
AclI AA'CG_TT
Cuts at: 1563 3822 4195 1563
Size: 2259 373 3063
Fragments arranged by size:
3063 2259 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2675 3758 5142 5410 1632
Size: 1043 1083 1384 268 1917
Fragments arranged by size:
1917 1384 1083 1043 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5498
AflIII A'CryG_T
Cuts at: 1203 2552 4594 5571 1203
Size: 1349 2042 977 1327
Fragments arranged by size:
2042 1349 1327 977
AgeI A'CCGG_T
Cuts at: 4526 4526
Size: 5695
AhdI GACnn_n'nnGTC
Cuts at: 4424 4424
Size: 5695
AleI CACnn'nnGTG
Cuts at: 1166 2162 1166
Size: 996 4699
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2313 2648 1184
Size: 1129 335 4231
Fragments arranged by size:
4231 1129 335
AlwNI CAG_nnn'CTG
Cuts at: 3438 5010 3438
Size: 1572 4123
ApaI G_GGCC'C
Cuts at: 2081 2094 2081
Size: 13 5682
ApaLI G'TGCA_C
Cuts at: 3752 4908 3752
Size: 1156 4539
ApoI r'AATT_y
Cuts at: 1493 3004 3230 3382 5491 1493
Size: 1511 226 152 2109 1697
Fragments arranged by size:
2109 1697 1511 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4249 160
Size: 872 3217 1606
Fragments arranged by size:
3217 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5695
AvaI C'yCGr_G
Cuts at: 1870 1943 3020 1870
Size: 73 1077 4545
Fragments arranged by size:
4545 1077 73
BamHI G'GATC_C
Cuts at: 3025 3392 3025
Size: 367 5328
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2156 2492 2697 3016 4471
Size: 325 217 627 369 336 205 319 1455
Cuts at: 4471 618
Size: 1842
Fragments arranged by size:
1842 1455 627 369 336 325 319 217
205
BanII G_rGCy'C
Cuts at: 1391 2081 2094 3014 1391
Size: 690 13 920 4072
Fragments arranged by size:
4072 920 690 13
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2296 928
Size: 971 397 4327
Fragments arranged by size:
4327 971 397
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3886 3920 1903
Size: 34 1949 34 3678
Fragments arranged by size:
3678 1949 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3587 4797 3587
Size: 1210 4485
BclI T'GATC_A
Cuts at: 1548 2877 1548
Size: 1329 4366
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2541 2750 4306
Size: 107 122 71 631 605 869 209 1556
Cuts at: 4306 136
Size: 1525
Fragments arranged by size:
1556 1525 869 631 605 209 122 107
71
BglII A'GATC_T
Cuts at: 5690 5690
Size: 5695
BlpI GC'TnA_GC
Cuts at: 2317 3112 2317
Size: 795 4900
Bme1580I G_kGCm'C
Cuts at: 1790 2081 2094 2207 2224 2497 3756 4912 1790
Size: 291 13 113 17 273 1259 1156 2573
Fragments arranged by size:
2573 1259 1156 291 273 113 17 13
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5695
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2979 3426 4384
Size: 416 53 83 399 24 1562 447 958
Cuts at: 4384 442
Size: 1753
Fragments arranged by size:
1753 1562 958 447 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5695
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4355 1239
Size: 3116 2579
Bpu10I CC'TnA_GC
Cuts at: 2050 2050
Size: 5695
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3820 4685 4983 3820
Size: 865 298 4532
Fragments arranged by size:
4532 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4358 882
Size: 617 2859 2219
Fragments arranged by size:
2859 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3486 493
Size: 711 2282 2702
Fragments arranged by size:
2702 2282 711
BsaBI GATnn'nnATC
Cuts at: 5689 5689
Size: 5695
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3502 3884 4519 275
Size: 53 83 186 936 1969 382 635 1451
Fragments arranged by size:
1969 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4128 4526 4800 4947 5358 4128
Size: 398 274 147 411 4465
Fragments arranged by size:
4465 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5470
Fragments arranged by size:
5470 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5695
BseYI C'CCAG_C
Cuts at: 2080 2676 2809 4898 5394 2080
Size: 596 133 2089 496 2381
Fragments arranged by size:
2381 2089 596 496 133
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2037 2273 2936 1163
Size: 573 301 236 663 3922
Fragments arranged by size:
3922 663 573 301 236
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2404 3145 3906 4934 1540
Size: 275 186 403 741 761 1028 2301
Fragments arranged by size:
2301 1028 761 741 403 275 186
BsiHKAI G_wGCw'C
Cuts at: 2145 3756 3841 4912 2145
Size: 1611 85 1071 2928
Fragments arranged by size:
2928 1611 1071 85
BsmI GAATG_Cn'
Cuts at: 2182 3206 3299 2182
Size: 1024 93 4578
Fragments arranged by size:
4578 1024 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2081 2094 2145 2207 2224 2497 3014
Size: 399 291 13 51 62 17 273 517
Cuts at: 3014 3756 3841 4912 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 517 399 291 273 85
62 51 17 13
BspEI T'CCGG_A
Cuts at: 5358 5358
Size: 5695
BspHI T'CATG_A
Cuts at: 3584 5314 3584
Size: 1730 3965
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2271 2709 3050 844
Size: 1427 438 341 3489
Fragments arranged by size:
3489 1427 438 341
BsrBI CCG'CTC
Cuts at: 1394 1823 2979 3582 1394
Size: 429 1156 603 3507
Fragments arranged by size:
3507 1156 603 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2361 2601 2739 4190 4364 65
Size: 1183 1113 240 138 1451 174 1396
Fragments arranged by size:
1451 1396 1183 1113 240 174 138
BsrFI r'CCGG_y
Cuts at: 1700 1810 4339 4526 1700
Size: 110 2529 187 2869
Fragments arranged by size:
2869 2529 187 110
BsrGI T'GTAC_A
Cuts at: 96 2418 96
Size: 2322 3373
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5695
BssSI C'ACGA_G
Cuts at: 3755 4767 3755
Size: 1012 4683
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2664 2704 2923 794
Size: 1870 40 219 3566
Fragments arranged by size:
3566 1870 219 40
BstBI TT'CG_AA
Cuts at: 4499 4499
Size: 5695
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5695
BstXI CCAn_nnnn'nTGG
Cuts at: 2200 2200
Size: 5695
BstYI r'GATC_y
Cuts at: 1972 2961 3025 3392 3777 3794 5246 5332 5344
Size: 989 64 367 385 17 1452 86 12
Cuts at: 5344 5690 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 989 385 367 346 86 64
17 12
Bsu36I CC'TnA_GG
Cuts at: 2124 2124
Size: 5695
BtgI C'CryG_G
Cuts at: 513 1065 2253 2754 513
Size: 552 1188 501 3454
Fragments arranged by size:
3454 1188 552 501
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4956
Fragments arranged by size:
4956 683 56
BtsI GCAGTG_nn'
Cuts at: 2111 3211 4005 4025 2111
Size: 1100 794 20 3781
Fragments arranged by size:
3781 1100 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5695
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5660
DraI TTT'AAA
Cuts at: 2999 2999
Size: 5695
DraIII CAC_nnn'GTG
Cuts at: 2722 2722
Size: 5695
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4702 809
Size: 507 3386 1802
Fragments arranged by size:
3386 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2190 2250 2388
Size: 54 1425 50 275 18 360 60 138
Cuts at: 2388 2598 2724 2751 3142 4031 5660 8
Size: 210 126 27 391 889 1629 43
Fragments arranged by size:
1629 1425 889 391 360 275 210 138
126 60 54 50 43 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 3142 1537
Size: 275 1330 4090
Fragments arranged by size:
4090 1330 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2959 3625 1353
Size: 1606 666 3423
Fragments arranged by size:
3423 1606 666
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4282 4656 4802 387
Size: 1064 138 2693 374 146 1280
Fragments arranged by size:
2693 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2675 3758 4355 5142 5410 1239
Size: 393 1043 1083 597 787 268 1524
Fragments arranged by size:
1524 1083 1043 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2090 2090
Size: 5695
EcoRI G'AATT_C
Cuts at: 3004 3382 3004
Size: 378 5317
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2983 2983
Size: 5695
FspI TGC'GCA
Cuts at: 4201 4201
Size: 5695
HaeII r_GCGC'y
Cuts at: 1678 4842 1678
Size: 3164 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2807 2839 4336 4368 4410
Size: 32 135 32 1235 32 1497 32 42
Cuts at: 4410 4442 1373
Size: 32 2626
Fragments arranged by size:
2626 1497 1235 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2401 3039 3285 669
Size: 988 281 463 638 246 3079
Fragments arranged by size:
3079 988 638 463 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5695
HpaI GTT'AAC
Cuts at: 3285 3285
Size: 5695
KpnI G_GTAC'C
Cuts at: 3020 3020
Size: 5695
MfeI C'AATT_G
Cuts at: 3294 3294
Size: 5695
MluI A'CGCG_T
Cuts at: 5571 5571
Size: 5695
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4809 4993 1305
Size: 3504 184 2007
Fragments arranged by size:
3504 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2192 2390 2600 2726 10
Size: 54 1425 343 360 198 210 126 2979
Fragments arranged by size:
2979 1425 360 343 210 198 126 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2162 2198 3653 4012 4171 518
Size: 648 996 36 1455 359 159 2042
Fragments arranged by size:
2042 1455 996 648 359 159 36
MspA1I CmG'CkG
Cuts at: 1606 2045 2321 2695 3111 3788 4936 5181 1606
Size: 439 276 374 416 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 439 416 374 276 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5143
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5695
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5695
NotI GC'GGCC_GC
Cuts at: 3142 3142
Size: 5695
NspI r_CATG'y
Cuts at: 1132 1229 2556 3053 4598 1132
Size: 97 1327 497 1545 2229
Fragments arranged by size:
2229 1545 1327 497 97
PasI CC'CwG_GG
Cuts at: 1348 2497 1348
Size: 1149 4546
PciI A'CATG_T
Cuts at: 2552 4594 2552
Size: 2042 3653
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 2722 1108
Size: 902 712 4081
Fragments arranged by size:
4081 902 712
PmeI GTTT'AAAC
Cuts at: 2999 2999
Size: 5695
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3764 3796 5277 5309 3764
Size: 32 1481 32 4150
Fragments arranged by size:
4150 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5695
PsiI TTA'TAA
Cuts at: 3265 3265
Size: 5695
PspOMI G'GGCC_C
Cuts at: 2077 2090 2077
Size: 13 5682
PstI C_TGCA'G
Cuts at: 830 2637 3047 830
Size: 1807 410 3478
Fragments arranged by size:
3478 1807 410
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5695
PvuII CAG'CTG
Cuts at: 2045 2045
Size: 5695
SalI G'TCGA_C
Cuts at: 1936 2399 3037 1936
Size: 463 638 4594
Fragments arranged by size:
4594 638 463
SbfI CC_TGCA'GG
Cuts at: 3047 3047
Size: 5695
ScaI AGT'ACT
Cuts at: 3943 5655 3943
Size: 1712 3983
SfcI C'TryA_G
Cuts at: 826 1045 2633 3043 4178 4859 5050 826
Size: 219 1588 410 1135 681 191 1471
Fragments arranged by size:
1588 1471 1135 681 410 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5695
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5695
SmaI CCC'GGG
Cuts at: 1872 3022 1872
Size: 1150 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3799 4700 4962 820
Size: 197 926 1856 901 262 1553
Fragments arranged by size:
1856 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3486 493
Size: 2993 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5695
SphI G_CATG'C
Cuts at: 1132 3053 1132
Size: 1921 3774
SspI AAT'ATT
Cuts at: 5 52 3619 5
Size: 47 3567 2081
Fragments arranged by size:
3567 2081 47
StuI AGG'CCT
Cuts at: 1517 2129 2274 1517
Size: 612 145 4938
Fragments arranged by size:
4938 612 145
StyI C'CwwG_G
Cuts at: 513 1065 3134 513
Size: 552 2069 3074
Fragments arranged by size:
3074 2069 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2801 3722 3739 3892 4077 4522 325
Size: 1255 1221 921 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1221 921 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2418 3941 5653
Size: 274 80 33 51 1443 441 1523 1712
Cuts at: 5653 96
Size: 138
Fragments arranged by size:
1712 1523 1443 441 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5655 5687 5655
Size: 32 5663
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5695
XbaI T'CTAG_A
Cuts at: 3031 3031
Size: 5695
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2233 2557 2584 2752 2233
Size: 324 27 168 5176
Fragments arranged by size:
5176 324 168 27
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5695
XmaI C'CCGG_G
Cuts at: 1870 3020 1870
Size: 1150 4545
XmnI GAAnn'nnTTC
Cuts at: 3824 3824
Size: 5695
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3503 276
Size: 53 83 186 936 1969 2468
Fragments arranged by size:
2468 1969 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI