Restriction map for
pFN21AB8786
(Circular) (Six-base) MAPSORT of: pFN21AB8786.seq Check: 1327 from: 1 to: 5596
pFN21AB8786 5596 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3406 278
Size: 53 83 186 936 1870 2468
Fragments arranged by size:
2468 1870 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2939 1937
Size: 1002 4594
Acc65I G'GTAC_C
Cuts at: 2415 2917 2415
Size: 502 5094
AclI AA'CG_TT
Cuts at: 1563 3723 4096 1563
Size: 2160 373 3063
Fragments arranged by size:
3063 2160 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2344 3659 5043 5311 1632
Size: 712 1315 1384 268 1917
Fragments arranged by size:
1917 1384 1315 712 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5399
AflIII A'CryG_T
Cuts at: 1203 2589 2766 4495 5472 1203
Size: 1386 177 1729 977 1327
Fragments arranged by size:
1729 1386 1327 977 177
AgeI A'CCGG_T
Cuts at: 4427 4427
Size: 5596
AhdI GACnn_n'nnGTC
Cuts at: 4325 4325
Size: 5596
AleI CACnn'nnGTG
Cuts at: 1166 2447 2498 1166
Size: 1281 51 4264
Fragments arranged by size:
4264 1281 51
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5596
AlwNI CAG_nnn'CTG
Cuts at: 2892 3339 4911 2892
Size: 447 1572 3577
Fragments arranged by size:
3577 1572 447
ApaI G_GGCC'C
Cuts at: 2704 2704
Size: 5596
ApaLI G'TGCA_C
Cuts at: 2585 3653 4809 2585
Size: 1068 1156 3372
Fragments arranged by size:
3372 1156 1068
ApoI r'AATT_y
Cuts at: 1493 2905 3131 3283 5392 1493
Size: 1412 226 152 2109 1697
Fragments arranged by size:
2109 1697 1412 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4150 160
Size: 872 3118 1606
Fragments arranged by size:
3118 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5596
AvaI C'yCGr_G
Cuts at: 1870 1943 2678 2921 1870
Size: 73 735 243 4545
Fragments arranged by size:
4545 735 243 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2399 2432 2399
Size: 33 5563
BamHI G'GATC_C
Cuts at: 2926 3293 2926
Size: 367 5229
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2173 2415 2917 4372 618
Size: 325 217 627 386 242 502 1455 1842
Fragments arranged by size:
1842 1455 627 502 386 325 242 217
BanII G_rGCy'C
Cuts at: 1391 2704 2846 2853 2915 1391
Size: 1313 142 7 62 4072
Fragments arranged by size:
4072 1313 142 62 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2557 2677 928
Size: 971 658 120 3847
Fragments arranged by size:
3847 971 658 120
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3787 3821 1903
Size: 34 1850 34 3678
Fragments arranged by size:
3678 1850 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3488 4698 3488
Size: 1210 4386
BclI T'GATC_A
Cuts at: 1548 2253 2529 2796 1548
Size: 705 276 267 4348
Fragments arranged by size:
4348 705 276 267
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4207 136
Size: 107 122 71 631 605 2535 1525
Fragments arranged by size:
2535 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2235 2622 5591 2235
Size: 387 2969 2240
Fragments arranged by size:
2969 2240 387
BlpI GC'TnA_GC
Cuts at: 3013 3013
Size: 5596
Bme1580I G_kGCm'C
Cuts at: 1790 2523 2589 2704 3657 4813 1790
Size: 733 66 115 953 1156 2573
Fragments arranged by size:
2573 1156 953 733 115 66
BmgBI CAC'GTC
Cuts at: 1318 2541 1318
Size: 1223 4373
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2902 3327 4285
Size: 416 53 83 399 24 1485 425 958
Cuts at: 4285 442
Size: 1753
Fragments arranged by size:
1753 1485 958 425 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5596
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2153 4256 1239
Size: 914 2103 2579
Fragments arranged by size:
2579 2103 914
Bpu10I CC'TnA_GC
Cuts at: 2852 2852
Size: 5596
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2264 3721 4586 4884 2264
Size: 1457 865 298 2976
Fragments arranged by size:
2976 1457 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4259 882
Size: 617 2760 2219
Fragments arranged by size:
2760 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2288 3387 493
Size: 711 1084 1099 2702
Fragments arranged by size:
2702 1099 1084 711
BsaBI GATnn'nnATC
Cuts at: 5590 5590
Size: 5596
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3403 3785 4420 275
Size: 53 83 186 936 1870 382 635 1451
Fragments arranged by size:
1870 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2838 4029 4427 4701 4848 5259 2838
Size: 1191 398 274 147 411 3175
Fragments arranged by size:
3175 1191 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5371
Fragments arranged by size:
5371 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2102 1186
Size: 916 4680
BseYI C'CCAG_C
Cuts at: 2218 2697 4799 5295 2218
Size: 479 2102 496 2519
Fragments arranged by size:
2519 2102 496 479
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2461 1163
Size: 573 725 4298
Fragments arranged by size:
4298 725 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3046 3807 4835 1540
Size: 275 186 1045 761 1028 2301
Fragments arranged by size:
2301 1045 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2589 2853 3657 3742 4813 2589
Size: 264 804 85 1071 3372
Fragments arranged by size:
3372 1071 804 264 85
BsmI GAATG_Cn'
Cuts at: 2861 3107 3200 2861
Size: 246 93 5257
Fragments arranged by size:
5257 246 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2523 2589 2704 2846 2853 2915 3657
Size: 399 733 66 115 142 7 62 742
Cuts at: 3657 3742 4813 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 733 399 142 115 85
66 62 7
BspEI T'CCGG_A
Cuts at: 2838 5259 2838
Size: 2421 3175
BspHI T'CATG_A
Cuts at: 3485 5215 3485
Size: 1730 3866
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2877 2951 844
Size: 2033 74 3489
Fragments arranged by size:
3489 2033 74
BsrBI CCG'CTC
Cuts at: 1394 1823 3483 1394
Size: 429 1660 3507
Fragments arranged by size:
3507 1660 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2812 4091 4265 65
Size: 1183 1564 1279 174 1396
Fragments arranged by size:
1564 1396 1279 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4240 4427 1700
Size: 110 2430 187 2869
Fragments arranged by size:
2869 2430 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5596
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5596
BssSI C'ACGA_G
Cuts at: 2070 3656 4668 2070
Size: 1586 1012 2998
Fragments arranged by size:
2998 1586 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5596
BstBI TT'CG_AA
Cuts at: 4400 4400
Size: 5596
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5596
BstYI r'GATC_y
Cuts at: 1972 2235 2622 2926 3293 3678 3695 5147 5233
Size: 263 387 304 367 385 17 1452 86
Cuts at: 5233 5245 5591 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 387 385 367 346 304 263
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2496 513
Size: 552 1431 3613
Fragments arranged by size:
3613 1431 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4857
Fragments arranged by size:
4857 683 56
BtsI GCAGTG_nn'
Cuts at: 2408 3112 3906 3926 2408
Size: 704 794 20 4078
Fragments arranged by size:
4078 794 704 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5596
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2269 2304 529
Size: 35 1705 35 3821
Fragments arranged by size:
3821 1705 35 35
DraI TTT'AAA
Cuts at: 2900 2900
Size: 5596
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4603 809
Size: 507 3287 1802
Fragments arranged by size:
3287 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2202 3043 3932
Size: 54 1425 50 275 18 372 841 889
Cuts at: 3932 5561 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 841 372 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3043 1537
Size: 275 1231 4090
Fragments arranged by size:
4090 1231 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2780 3526 1353
Size: 1427 746 3423
Fragments arranged by size:
3423 1427 746
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4183 4557 4703 387
Size: 1064 138 2594 374 146 1280
Fragments arranged by size:
2594 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2851 2851
Size: 5596
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2153 2344 3659 4256 5043 5311 1239
Size: 393 521 191 1315 597 787 268 1524
Fragments arranged by size:
1524 1315 787 597 521 393 268 191
EcoO109I rG'GnC_Cy
Cuts at: 2701 2701
Size: 5596
EcoRI G'AATT_C
Cuts at: 2905 3283 2905
Size: 378 5218
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2893 2893
Size: 5596
FspI TGC'GCA
Cuts at: 4102 4102
Size: 5596
HaeII r_GCGC'y
Cuts at: 1678 4743 1678
Size: 3065 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4237 4269 4311 4343 1373
Size: 32 135 32 2665 32 42 32 2626
Fragments arranged by size:
2665 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2940 3186 669
Size: 988 281 1002 246 3079
Fragments arranged by size:
3079 1002 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5596
HpaI GTT'AAC
Cuts at: 3186 3186
Size: 5596
KpnI G_GTAC'C
Cuts at: 2419 2921 2419
Size: 502 5094
MfeI C'AATT_G
Cuts at: 3195 3195
Size: 5596
MluI A'CGCG_T
Cuts at: 5472 5472
Size: 5596
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2085 2095 4710 4894 1305
Size: 780 10 2615 184 2007
Fragments arranged by size:
2615 2007 780 184 10
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2204 10
Size: 54 1425 343 372 3402
Fragments arranged by size:
3402 1425 372 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2447 2495 2498 3554 3913 4072 518
Size: 648 1281 48 3 1056 359 159 2042
Fragments arranged by size:
2042 1281 1056 648 359 159 48 3
MspA1I CmG'CkG
Cuts at: 1606 2554 3012 3689 4837 5082 1606
Size: 948 458 677 1148 245 2120
Fragments arranged by size:
2120 1148 948 677 458 245
NcoI C'CATG_G
Cuts at: 513 1065 2496 513
Size: 552 1431 3613
Fragments arranged by size:
3613 1431 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5596
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5596
NotI GC'GGCC_GC
Cuts at: 3043 3043
Size: 5596
NspI r_CATG'y
Cuts at: 1132 1229 2593 2770 2794 2954 4499 1132
Size: 97 1364 177 24 160 1545 2229
Fragments arranged by size:
2229 1545 1364 177 160 97 24
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5596
PciI A'CATG_T
Cuts at: 2589 2766 4495 2589
Size: 177 1729 3690
Fragments arranged by size:
3690 1729 177
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5596
PmeI GTTT'AAAC
Cuts at: 2900 2900
Size: 5596
PmlI CAC'GTG
Cuts at: 2288 2288
Size: 5596
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3665 3697 5178 5210 3665
Size: 32 1481 32 4051
Fragments arranged by size:
4051 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5596
PsiI TTA'TAA
Cuts at: 3166 3166
Size: 5596
PspOMI G'GGCC_C
Cuts at: 2700 2700
Size: 5596
PstI C_TGCA'G
Cuts at: 830 2885 2948 830
Size: 2055 63 3478
Fragments arranged by size:
3478 2055 63
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5596
RsrII CG'GwC_CG
Cuts at: 2383 2383
Size: 5596
SacI G_AGCT'C
Cuts at: 2853 2853
Size: 5596
SalI G'TCGA_C
Cuts at: 1936 2938 1936
Size: 1002 4594
SbfI CC_TGCA'GG
Cuts at: 2948 2948
Size: 5596
ScaI AGT'ACT
Cuts at: 3844 5556 3844
Size: 1712 3884
SfcI C'TryA_G
Cuts at: 826 1045 2194 2881 2944 4079 4760 4951 826
Size: 219 1149 687 63 1135 681 191 1471
Fragments arranged by size:
1471 1149 1135 687 681 219 191 63
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5596
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5596
SmaI CCC'GGG
Cuts at: 1872 2923 1872
Size: 1051 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2279 3700 4601 4863 820
Size: 197 926 336 1421 901 262 1553
Fragments arranged by size:
1553 1421 926 901 336 262 197
SnaBI TAC'GTA
Cuts at: 493 3387 493
Size: 2894 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5596
SphI G_CATG'C
Cuts at: 1132 2954 1132
Size: 1822 3774
SspI AAT'ATT
Cuts at: 5 52 3520 5
Size: 47 3468 2081
Fragments arranged by size:
3468 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5596
StyI C'CwwG_G
Cuts at: 513 1065 2496 3035 513
Size: 552 1431 539 3074
Fragments arranged by size:
3074 1431 552 539
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2191 3623 3640 3793 3978 4423 325
Size: 1255 611 1432 17 153 185 445 1498
Fragments arranged by size:
1498 1432 1255 611 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 3842 5554
Size: 274 80 33 51 1443 204 1661 1712
Cuts at: 5554 96
Size: 138
Fragments arranged by size:
1712 1661 1443 274 204 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2655 2687 5556 5588 2655
Size: 32 2869 32 2663
Fragments arranged by size:
2869 2663 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5596
XbaI T'CTAG_A
Cuts at: 2932 2932
Size: 5596
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5596
XmaI C'CCGG_G
Cuts at: 1870 2921 1870
Size: 1051 4545
XmnI GAAnn'nnTTC
Cuts at: 2056 3725 2056
Size: 1669 3927
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3404 276
Size: 53 83 186 936 1870 2468
Fragments arranged by size:
2468 1870 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspOMI PstI PvuI RsrII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PpuMI PspXI PsrI PvuII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI