Restriction map for
pFN21AB8778
(Circular) (Six-base) MAPSORT of: pFN21AB8778.seq Check: 4677 from: 1 to: 5479
pFN21AB8778 5479 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2329 2329
Size: 5479
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3289 278
Size: 53 83 186 936 1753 2468
Fragments arranged by size:
2468 1753 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2822 1937
Size: 885 4594
Acc65I G'GTAC_C
Cuts at: 2800 2800
Size: 5479
AclI AA'CG_TT
Cuts at: 1563 3606 3979 1563
Size: 2043 373 3063
Fragments arranged by size:
3063 2043 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3542 4926 5194 1632
Size: 1910 1384 268 1917
Fragments arranged by size:
1917 1910 1384 268
AfeI AGC'GCT
Cuts at: 2297 2297
Size: 5479
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5282
AflIII A'CryG_T
Cuts at: 1203 2175 4378 5355 1203
Size: 972 2203 977 1327
Fragments arranged by size:
2203 1327 977 972
AgeI A'CCGG_T
Cuts at: 4310 4310
Size: 5479
AhdI GACnn_n'nnGTC
Cuts at: 4208 4208
Size: 5479
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5479
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5479
AlwNI CAG_nnn'CTG
Cuts at: 2194 2344 2558 3222 4794 2194
Size: 150 214 664 1572 2879
Fragments arranged by size:
2879 1572 664 214 150
ApaI G_GGCC'C
Cuts at: 2133 2515 2679 2133
Size: 382 164 4933
Fragments arranged by size:
4933 382 164
ApaLI G'TGCA_C
Cuts at: 3536 4692 3536
Size: 1156 4323
ApoI r'AATT_y
Cuts at: 1493 2788 3014 3166 5275 1493
Size: 1295 226 152 2109 1697
Fragments arranged by size:
2109 1697 1295 226 152
AscI GG'CGCG_CC
Cuts at: 2261 2261
Size: 5479
AseI AT'TA_AT
Cuts at: 160 1032 4033 160
Size: 872 3001 1606
Fragments arranged by size:
3001 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5479
AvaI C'yCGr_G
Cuts at: 1870 1943 2126 2146 2165 2508 2532 2804 1870
Size: 73 183 20 19 343 24 272 4545
Fragments arranged by size:
4545 343 272 183 73 24 20 19
BamHI G'GATC_C
Cuts at: 2809 3176 2809
Size: 367 5112
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2429 2800 4255 618
Size: 325 217 627 642 371 1455 1842
Fragments arranged by size:
1842 1455 642 627 371 325 217
BanII G_rGCy'C
Cuts at: 1391 2133 2252 2515 2679 2712 2798 1391
Size: 742 119 263 164 33 86 4072
Fragments arranged by size:
4072 742 263 164 119 86 33
BbeI G_GCGC'C
Cuts at: 2433 2433
Size: 5479
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2618 928
Size: 971 719 3789
Fragments arranged by size:
3789 971 719
BbvCI CC'TCA_GC
Cuts at: 2159 2159
Size: 5479
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3670 3704 1903
Size: 34 1733 34 3678
Fragments arranged by size:
3678 1733 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3371 4581 3371
Size: 1210 4269
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5479
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2210 4090 136
Size: 107 122 71 631 605 538 1880 1525
Fragments arranged by size:
1880 1525 631 605 538 122 107 71
BglII A'GATC_T
Cuts at: 5474 5474
Size: 5479
BlpI GC'TnA_GC
Cuts at: 2896 2896
Size: 5479
Bme1580I G_kGCm'C
Cuts at: 1790 2133 2451 2515 2679 3540 4696 1790
Size: 343 318 64 164 861 1156 2573
Fragments arranged by size:
2573 1156 861 343 318 164 64
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5479
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3210 4168 442
Size: 416 53 83 399 24 1793 958 1753
Fragments arranged by size:
1793 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2106 1056
Size: 1050 4429
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2378 4139 1239
Size: 1139 1761 2579
Fragments arranged by size:
2579 1761 1139
Bpu10I CC'TnA_GC
Cuts at: 2159 2159
Size: 5479
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3604 4469 4767 3604
Size: 865 298 4316
Fragments arranged by size:
4316 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2711 4142 882
Size: 617 1212 1431 2219
Fragments arranged by size:
2219 1431 1212 617
BsaAI yAC'GTr
Cuts at: 493 1204 3270 493
Size: 711 2066 2702
Fragments arranged by size:
2702 2066 711
BsaBI GATnn'nnATC
Cuts at: 5473 5473
Size: 5479
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2430 3286 3668 4303
Size: 53 83 186 936 897 856 382 635
Cuts at: 4303 275
Size: 1451
Fragments arranged by size:
1451 936 897 856 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2290 3912 4310 4584 4731 5142 2290
Size: 1622 398 274 147 411 2627
Fragments arranged by size:
2627 1622 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2359 2389 2528 2554 2558
Size: 30 165 30 956 30 139 26 4
Cuts at: 2558 2584 1178
Size: 26 4073
Fragments arranged by size:
4073 956 165 139 30 30 30 26
26 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2001 2060 2134 2669 1186
Size: 815 59 74 535 3996
Fragments arranged by size:
3996 815 535 74 59
BseYI C'CCAG_C
Cuts at: 2344 2551 2558 4682 5178 2344
Size: 207 7 2124 496 2645
Fragments arranged by size:
2645 2124 496 207 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2200 1163
Size: 573 464 4442
Fragments arranged by size:
4442 573 464
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2929 3690 4718 1540
Size: 275 186 928 761 1028 2301
Fragments arranged by size:
2301 1028 928 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2712 3540 3625 4696 2712
Size: 828 85 1071 3495
Fragments arranged by size:
3495 1071 828 85
BsmI GAATG_Cn'
Cuts at: 2990 3083 2990
Size: 93 5386
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2133 2252 2451 2515 2679 2712 2798
Size: 399 343 119 199 64 164 33 86
Cuts at: 2798 3540 3625 4696 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 343 199 164 119
86 85 64 33
BspEI T'CCGG_A
Cuts at: 2290 5142 2290
Size: 2852 2627
BspHI T'CATG_A
Cuts at: 3368 5098 3368
Size: 1730 3749
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2329 2834 844
Size: 1485 505 3489
Fragments arranged by size:
3489 1485 505
BsrBI CCG'CTC
Cuts at: 1394 1823 2315 2392 3366 1394
Size: 429 492 77 974 3507
Fragments arranged by size:
3507 974 492 429 77
BsrDI GCAATG_nn'
Cuts at: 65 1248 3974 4148 65
Size: 1183 2726 174 1396
Fragments arranged by size:
2726 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2119 4123 4310 1700
Size: 110 309 2004 187 2869
Fragments arranged by size:
2869 2004 309 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5479
BssHII G'CGCG_C
Cuts at: 1925 2261 1925
Size: 336 5143
BssSI C'ACGA_G
Cuts at: 3539 4551 3539
Size: 1012 4467
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2194 794
Size: 1400 4079
BstBI TT'CG_AA
Cuts at: 4283 4283
Size: 5479
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5479
BstYI r'GATC_y
Cuts at: 1972 2809 3176 3561 3578 5030 5116 5128 5474
Size: 837 367 385 17 1452 86 12 346
Cuts at: 5474 1972
Size: 1977
Fragments arranged by size:
1977 1452 837 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2281 2425 2664 513
Size: 552 1216 144 239 3328
Fragments arranged by size:
3328 1216 552 239 144
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4740
Fragments arranged by size:
4740 683 56
BtsI GCAGTG_nn'
Cuts at: 2995 3789 3809 2995
Size: 794 20 4665
Fragments arranged by size:
4665 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5479
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5444
DraI TTT'AAA
Cuts at: 2783 2783
Size: 5479
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4486 809
Size: 507 3170 1802
Fragments arranged by size:
3170 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2926 3815 5444
Size: 54 1425 50 275 18 1096 889 1629
Cuts at: 5444 8
Size: 43
Fragments arranged by size:
1629 1425 1096 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2926 1537
Size: 275 1114 4090
Fragments arranged by size:
4090 1114 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3409 1353
Size: 2056 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2129 2542 4066 4440 4586 387
Size: 1064 138 540 413 1524 374 146 1280
Fragments arranged by size:
1524 1280 1064 540 413 374 146 138
EcoICRI GAG'CTC
Cuts at: 2710 2710
Size: 5479
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2378 3542 4139 4926 5194 1239
Size: 393 746 1164 597 787 268 1524
Fragments arranged by size:
1524 1164 787 746 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2605 2605
Size: 5479
EcoO109I rG'GnC_Cy
Cuts at: 2370 2562 2370
Size: 192 5287
EcoRI G'AATT_C
Cuts at: 2788 3166 2788
Size: 378 5101
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2656 2656
Size: 5479
FspI TGC'GCA
Cuts at: 2302 2357 3985 2302
Size: 55 1628 3796
Fragments arranged by size:
3796 1628 55
HaeII r_GCGC'y
Cuts at: 1678 2237 2299 2433 4626 1678
Size: 559 62 134 2193 2531
Fragments arranged by size:
2531 2193 559 134 62
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2359 2391 2528 2554 2560
Size: 32 135 32 787 32 137 26 6
Cuts at: 2560 2586 4120 4152 4194 4226 1373
Size: 26 1534 32 42 32 2626
Fragments arranged by size:
2626 1534 787 137 135 42 32 32
32 32 32 26 26 6
HincII GTy'rAC
Cuts at: 669 1657 1938 2823 3069 669
Size: 988 281 885 246 3079
Fragments arranged by size:
3079 988 885 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5479
HpaI GTT'AAC
Cuts at: 3069 3069
Size: 5479
KasI G'GCGC_C
Cuts at: 2429 2429
Size: 5479
KpnI G_GTAC'C
Cuts at: 2804 2804
Size: 5479
MfeI C'AATT_G
Cuts at: 3078 3078
Size: 5479
MluI A'CGCG_T
Cuts at: 2175 5355 2175
Size: 3180 2299
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4593 4777 1305
Size: 3288 184 2007
Fragments arranged by size:
3288 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3657
Fragments arranged by size:
3657 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3437 3796 3955 518
Size: 648 2271 359 159 2042
Fragments arranged by size:
2271 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2184 2219 2283 2558 2895 3572 4720 4965
Size: 578 35 64 275 337 677 1148 245
Cuts at: 4965 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 578 337 275 245 64
35
NarI GG'CG_CC
Cuts at: 2430 2430
Size: 5479
NcoI C'CATG_G
Cuts at: 513 1065 2664 513
Size: 552 1599 3328
Fragments arranged by size:
3328 1599 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5479
NheI G'CTAG_C
Cuts at: 1052 2102 1052
Size: 1050 4429
NotI GC'GGCC_GC
Cuts at: 2926 2926
Size: 5479
NspI r_CATG'y
Cuts at: 1132 1229 2837 4382 1132
Size: 97 1608 1545 2229
Fragments arranged by size:
2229 1608 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5479
PciI A'CATG_T
Cuts at: 4378 4378
Size: 5479
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2558 1108
Size: 1450 4029
PmeI GTTT'AAAC
Cuts at: 2783 2783
Size: 5479
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3548 3580 5061 5093 3548
Size: 32 1481 32 3934
Fragments arranged by size:
3934 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2562 2562
Size: 5479
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5479
PsiI TTA'TAA
Cuts at: 3049 3049
Size: 5479
PspOMI G'GGCC_C
Cuts at: 2129 2511 2675 2129
Size: 382 164 4933
Fragments arranged by size:
4933 382 164
PstI C_TGCA'G
Cuts at: 830 2831 830
Size: 2001 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5479
PvuII CAG'CTG
Cuts at: 2184 2184
Size: 5479
SacI G_AGCT'C
Cuts at: 2712 2712
Size: 5479
SacII CC_GC'GG
Cuts at: 2284 2284
Size: 5479
SalI G'TCGA_C
Cuts at: 1936 2821 1936
Size: 885 4594
SanDI GG'GwC_CC
Cuts at: 2562 2562
Size: 5479
SbfI CC_TGCA'GG
Cuts at: 2831 2831
Size: 5479
ScaI AGT'ACT
Cuts at: 3727 5439 3727
Size: 1712 3767
SfcI C'TryA_G
Cuts at: 826 1045 2827 3962 4643 4834 826
Size: 219 1782 1135 681 191 1471
Fragments arranged by size:
1782 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2431 2431
Size: 5479
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5479
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5479
SmaI CCC'GGG
Cuts at: 1872 2128 2510 2534 2806 1872
Size: 256 382 24 272 4545
Fragments arranged by size:
4545 382 272 256 24
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3583 4484 4746 820
Size: 197 926 1640 901 262 1553
Fragments arranged by size:
1640 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3270 493
Size: 2777 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5479
SphI G_CATG'C
Cuts at: 1132 2837 1132
Size: 1705 3774
SspI AAT'ATT
Cuts at: 5 52 3403 5
Size: 47 3351 2081
Fragments arranged by size:
3351 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5479
StyI C'CwwG_G
Cuts at: 513 1065 2640 2664 2918 513
Size: 552 1575 24 254 3074
Fragments arranged by size:
3074 1575 552 254 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3506 3523 3676 3861 4306 325
Size: 1255 1926 17 153 185 445 1498
Fragments arranged by size:
1926 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3725 5437 96
Size: 274 80 33 51 1443 1748 1712 138
Fragments arranged by size:
1748 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5439 5471 5439
Size: 32 5447
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5479
XbaI T'CTAG_A
Cuts at: 2815 2815
Size: 5479
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5479
XmaI C'CCGG_G
Cuts at: 1870 2126 2508 2532 2804 1870
Size: 256 382 24 272 4545
Fragments arranged by size:
4545 382 272 256 24
XmnI GAAnn'nnTTC
Cuts at: 3608 3608
Size: 5479
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3287 276
Size: 53 83 186 936 1753 2468
Fragments arranged by size:
2468 1753 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SanDI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI
BstZ17I Bsu36I DraIII EcoRV FseI FspAI NaeI NgoMIV
NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII
SapI SexAI SfiI SrfI SwaI XcmI